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Gene information
General information
TC006412 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8507620..8512064
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006412
    iBeetle screen iB_07492

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006412 RA
      MSSPVQESD CNTSPAPDP GTTHNNDPD VVATDSDSD 
      TDNPEASVA DRPTLTLKM LNLCKEFLI MIIIAVAYA 
      ALTNETPPK VSKSPAAYP FFPPNSKVT DWYRGQITE 
      AIEDARSSD IAFVMFYAP WDAESQTAR KQLEIAAQY 
      MQKEVAFVA VNCWQPGSE CKRQYSKVY KWPVLIAYP 
      THGRGIQYN GPLEAFHII KFLQNVCKP ILRPETVDE 
      LMGKHDTVV LGKINVLPG SYEYAVFYS TALRFLERD 
      PFRDTIFAV LPQKSDFDP SISLHVWNG TIDFKGELT 
      TESLTSWIL KNSHQITSW VSPTGMKSL SLSNFMQPG 
      PTLILFTPR NPLLQQTDH FDMLREAAY DYYNCANNS 
      HLTTRQKLI RESRLRNRK NYVKMTQKC HTRPPVITN 
      VPNIWTNNT DICNCVDKE SLNKFCESS VLAKPVLQQ 
      SVDRCRGFL LTQQYSQVV FESRGLEGG ANITGSACA 
      SNSSLKFVA IDSLRYYQF AERLGVGLG QKTGVVIVN 
      DKMETHYVM SGNVNSDNL RLFISNFTQ KTLPRSLRS 
      KVHVRTKSD HVYPHLNCS RSSQFCVRE LTTGDFLPT 
      VLQPNKVVV VFYYSKQCA FCSGFAYMF LTAARLLSP 
      VKDISFVRI DGDSNLLPW EYTMESFPT ILFFPSVRK 
      SESRAFPRN LPITVPNLL GFILTNIDP ILKLKTMWC 
      ICCQTKFPD EKIKCVTSL RMETLTIIG TTLREWRNS 
      SRKQKVLYK LQVLRQLHL SFAHSPEDV SQINTNLNK 
      LRD
    CDS sequence
    • >TC006412 RA
      atgtcatct ccggtacaa gagtcggac tgtaacacc 
      tcgccagca cctgaccca gggaccact cacaataat 
      gaccccgac gttgtcgcg acagattca gatagcgac 
      accgataat cccgaagcg agcgtggca gaccgtccc 
      acgttaacc ctcaaaatg ttgaacttg tgcaaagaa 
      ttcctgatc atgattatc atcgcggtg gcctacgct 
      gccctaact aacgagacc cctccgaaa gtgagcaaa 
      agtccagct gcgtacccg tttttccct ccaaattcc 
      aaagtgacg gactggtac cgggggcag atcactgag 
      gcgattgaa gatgcgcgc tcgagcgat attgcgttt 
      gtcatgttt tacgccccg tgggatgcc gagagccag 
      acggcgcgc aagcagctg gaaatcgca gcgcagtat 
      atgcagaaa gaggtggct tttgtggcg gttaactgc 
      tggcagcca ggcagcgag tgcaaaagg cagtacagc 
      aaagtgtac aagtggccc gttttgatc gcctatccc 
      acacacggg agagggata caatacaac gggccgctg 
      gaggctttc cacataatt aaatttttg caaaatgtg 
      tgcaagccc attttgcgg cctgagaca gttgacgaa 
      ctcatgggc aaacatgat accgttgta ttgggtaaa 
      ataaatgtt ctgcccggc agctacgaa tatgccgtt 
      ttttactca acagcattg aggttcctc gagagggac 
      cctttcagg gatacaatt tttgcagtc ctacctcaa 
      aaatctgac tttgacccc tccataagc ctccatgtt 
      tggaacgga acaattgat tttaaaggc gaattgacc 
      accgaatcc ctgacatcc tggattttg aaaaattca 
      catcaaatc accagttgg gtttccccc actggcatg 
      aagagcctc agtctctca aatttcatg caacccggc 
      cccaccctt attttattc actcctaga aacccactg 
      ttgcaacaa accgaccac tttgatatg ttgcgcgaa 
      gccgcctac gattattac aattgtgcc aacaattct 
      cacctcaca acacgacaa aaactgata agagaatca 
      cggttacga aaccgtaaa aattatgtt aaaatgacc 
      caaaaatgc cacactcgc ccaccggtg attacgaac 
      gtgcccaac atttggacg aacaatacg gatatttgt 
      aattgtgtg gacaaagag tccttgaat aaattttgc 
      gagagttcg gtcctggcc aagccggta ttacagcaa 
      tctgtagac cgctgtcgc ggtttccta ttgacgcaa 
      cagtacagc caggttgtt tttgagtca cgtggcctc 
      gaaggcggg gctaatatc acaggatcg gcttgtgca 
      agcaattca agtttgaag ttcgtagcg attgatagt 
      ttgaggtat tatcaattt gccgaacgg ttgggggtt 
      ggtttgggg cagaaaaca ggggttgtt atagttaat 
      gataagatg gagactcat tacgtgatg agtggtaac 
      gtaaatagt gataattta cgtttgttt atttctaat 
      tttacacaa aagacttta ccaagatct ctacggtcg 
      aaagtgcac gttaggacg aagtctgat catgtttat 
      cctcactta aattgctcc aggtcgagt cagttttgt 
      gtgagggaa ttaacaact ggcgatttt ttaccaact 
      gtgcttcaa cccaataaa gtcgtcgtt gttttttat 
      tattcgaaa caatgtgct ttttgtagc ggttttgcc 
      tacatgttt ttgacagca gccaggttg ttgagccca 
      gtcaaagac ataagtttt gtgaggata gacggcgat 
      tctaattta ctgccatgg gagtacact atggagagc 
      tttccgacg atattattt ttcccctct gtcagaaaa 
      tcggaatct cgcgccttt cccaggaac ctccccata 
      acggtgcca aacctcctg ggctttatt ctaacaaac 
      atcgacccc atcctcaaa ctcaaaaca atgtggtgt 
      atttgctgc caaaccaag ttccccgat gagaaaata 
      aaatgtgtc acttcactc cgtatggaa acactgacc 
      ataatcggg acaaccttg cgtgagtgg cgcaattcg 
      agccgaaag cagaaggtt ttatacaaa ttgcaagtt 
      ttgaggcaa ctgcatttg tcatttgct cactctccc 
      gaagatgtt tcgcaaatc aataccaat ctgaataaa 
      ttacgggat tga
    mRNA sequence
    • >TC006412 RA
      tgacagctg acgtgtgct gacacgatg tcaaatttc 
      gtaaaattt cgatttttc acctaacca aagagcaaa 
      attctccga atccgcggc aagtttacg tgaaatcga 
      gcaaggttc aatggtatg aggtcaact gggtatgtc 
      atctccggt acaagagtc ggactgtaa cacctcgcc 
      agcacctga cccagggac cactcacaa taatgaccc 
      cgacgttgt cgcgacaga ttcagatag cgacaccga 
      taatcccga agcgagcgt ggcagaccg tcccacgtt 
      aaccctcaa aatgttgaa cttgtgcaa agaattcct 
      gatcatgat tatcatcgc ggtggccta cgctgccct 
      aactaacga gacccctcc gaaagtgag caaaagtcc 
      agctgcgta cccgttttt ccctccaaa ttccaaagt 
      gacggactg gtaccgggg gcagatcac tgaggcgat 
      tgaagatgc gcgctcgag cgatattgc gtttgtcat 
      gttttacgc cccgtggga tgccgagag ccagacggc 
      gcgcaagca gctggaaat cgcagcgca gtatatgca 
      gaaagaggt ggcttttgt ggcggttaa ctgctggca 
      gccaggcag cgagtgcaa aaggcagta cagcaaagt 
      gtacaagtg gcccgtttt gatcgccta tcccacaca 
      cgggagagg gatacaata caacgggcc gctggaggc 
      tttccacat aattaaatt tttgcaaaa tgtgtgcaa 
      gcccatttt gcggcctga gacagttga cgaactcat 
      gggcaaaca tgataccgt tgtattggg taaaataaa 
      tgttctgcc cggcagcta cgaatatgc cgtttttta 
      ctcaacagc attgaggtt cctcgagag ggacccttt 
      cagggatac aatttttgc agtcctacc tcaaaaatc 
      tgactttga cccctccat aagcctcca tgtttggaa 
      cggaacaat tgattttaa aggcgaatt gaccaccga 
      atccctgac atcctggat tttgaaaaa ttcacatca 
      aatcaccag ttgggtttc ccccactgg catgaagag 
      cctcagtct ctcaaattt catgcaacc cggccccac 
      ccttatttt attcactcc tagaaaccc actgttgca 
      acaaaccga ccactttga tatgttgcg cgaagccgc 
      ctacgatta ttacaattg tgccaacaa ttctcacct 
      cacaacacg acaaaaact gataagaga atcacggtt 
      acgaaaccg taaaaatta tgttaaaat gacccaaaa 
      atgccacac tcgcccacc ggtgattac gaacgtgcc 
      caacatttg gacgaacaa tacggatat ttgtaattg 
      tgtggacaa agagtcctt gaataaatt ttgcgagag 
      ttcggtcct ggccaagcc ggtattaca gcaatctgt 
      agaccgctg tcgcggttt cctattgac gcaacagta 
      cagccaggt tgtttttga gtcacgtgg cctcgaagg 
      cggggctaa tatcacagg atcggcttg tgcaagcaa 
      ttcaagttt gaagttcgt agcgattga tagtttgag 
      gtattatca atttgccga acggttggg ggttggttt 
      ggggcagaa aacaggggt tgttatagt taatgataa 
      gatggagac tcattacgt gatgagtgg taacgtaaa 
      tagtgataa tttacgttt gtttatttc taattttac 
      acaaaagac tttaccaag atctctacg gtcgaaagt 
      gcacgttag gacgaagtc tgatcatgt ttatcctca 
      cttaaattg ctccaggtc gagtcagtt ttgtgtgag 
      ggaattaac aactggcga ttttttacc aactgtgct 
      tcaacccaa taaagtcgt cgttgtttt ttattattc 
      gaaacaatg tgctttttg tagcggttt tgcctacat 
      gtttttgac agcagccag gttgttgag cccagtcaa 
      agacataag ttttgtgag gatagacgg cgattctaa 
      tttactgcc atgggagta cactatgga gagctttcc 
      gacgatatt atttttccc ctctgtcag aaaatcgga 
      atctcgcgc ctttcccag gaacctccc cataacggt 
      gccaaacct cctgggctt tattctaac aaacatcga 
      ccccatcct caaactcaa aacaatgtg gtgtatttg 
      ctgccaaac caagttccc cgatgagaa aataaaatg 
      tgtcacttc actccgtat ggaaacact gaccataat 
      cgggacaac cttgcgtga gtggcgcaa ttcgagccg 
      aaagcagaa ggttttata caaattgca agttttgag 
      gcaactgca tttgtcatt tgctcactc tcccgaaga 
      tgtttcgca aatcaatac caatctgaa taaattacg 
      ggattgatt caggccaaa gccaagtgc acttaaaga 
      aacgaaaaa cattccgaa tttacgtat tttgggatg 
      tactcattt aggttttac ccttgtatc tgtgataat 
      aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY