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Gene information
General information
TC006409 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8498780..8500957
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006409
    iBeetle screen iB_07490

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: >5 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    mandible unilateral not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    mandible shape rounded - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006409 RA
      MPSPQQNES SPEPETSTS NDNKEKGAT VNEIMVIDP 
DTLELDLNH GRISKLENL EPLTQIERL FLRWNLITK 
IENLDTLVT LRELELYDN QIVKMENLG CLVNLEILD 
LSFNRIKEI EGLENLHNL EKLFLSSNK ISKIKNVKH 
LQKLTMLEL GDNKIRDIE NLENMPALT HLYLGKNKI 
SKIKNLDSL TNVTCLSLQ SNRITRIEN LDKLTQLTE 
LYISENGIH KIENLTHNT LLQTLDLAK NKIKLIENI 
EHLNDLEEF WMNDNEVSD WGTVENLSK NKKLATVYL 
ERNPLEKDP MYRRKLKMI APSLTQIDA TLCQL
    CDS sequence
    • >TC006409 RA
      atgccttct ccgcaacaa aacgagagt tcccccgag 
cccgagacg tccacttcg aacgacaat aaggaaaaa 
ggggccact gtcaacgaa ataatggta atagacccg 
gacaccctt gagctggac ctaaaccat ggcaggatc 
agcaagctc gaaaacctt gagcccttg acacaaatc 
gagcgcctc ttcctgagg tggaatttg atcaccaaa 
attgagaat ttggacacg ttagtcacg ctccgcgaa 
ttggaattg tacgataac caaatcgtc aaaatggaa 
aatctggga tgtttggtt aatttagag attctagat 
ttgtcgttt aatcgtatc aaggagatt gagggactg 
gagaatttg cacaattta gaaaaactc ttcctgtcg 
tcgaacaaa atcagcaaa atcaaaaat gtgaagcac 
ttgcagaag ctgacaatg ctggagctg ggggacaac 
aaaattagg gacatagag aacctggaa aacatgcca 
gccctgacc cacctctac ttgggcaaa aataaaata 
tcaaaaata aaaaacttg gacagtctg accaacgtg 
acgtgtctg agcctgcaa agcaaccga ataacgcgt 
atcgagaat ttggataag ctcacacaa ttgaccgaa 
ctgtacatt tccgagaac ggaattcat aaaatagaa 
aatcttacg cataacacg ttgctacag accttggat 
ttggcgaaa aataaaata aagttgatt gaaaacatc 
gagcatttg aacgacttg gaggagttt tggatgaat 
gataatgaa gtttccgat tggggaact gtcgagaat 
ttatcgaag aacaaaaag ctggctaca gtttacttg 
gagcggaat ccgctcgag aaggacccg atgtacagg 
aggaaattg aagatgatc gcgccctct ctaacccag 
atcgacgcg actttgtgt cagttgtaa
    mRNA sequence
    • >TC006409 RA
      ttgcgacca attgcaagt acagtttac tttaattaa 
cgtgtgaat aaggggtta aggggaaga cgatttgat 
ttaataggt tggctgcac gtgttgaag tcaaactca 
aacgtcacg gatgtttgc tgtcacccg cctttaaat 
tagtcactt aattaaatt gcaataaac agaaatagt 
ccagtgtga gtgcgaaaa atgccttct ccgcaacaa 
aacgagagt tcccccgag cccgagacg tccacttcg 
aacgacaat aaggaaaaa ggggccact gtcaacgaa 
ataatggta atagacccg gacaccctt gagctggac 
ctaaaccat ggcaggatc agcaagctc gaaaacctt 
gagcccttg acacaaatc gagcgcctc ttcctgagg 
tggaatttg atcaccaaa attgagaat ttggacacg 
ttagtcacg ctccgcgaa ttggaattg tacgataac 
caaatcgtc aaaatggaa aatctggga tgtttggtt 
aatttagag attctagat ttgtcgttt aatcgtatc 
aaggagatt gagggactg gagaatttg cacaattta 
gaaaaactc ttcctgtcg tcgaacaaa atcagcaaa 
atcaaaaat gtgaagcac ttgcagaag ctgacaatg 
ctggagctg ggggacaac aaaattagg gacatagag 
aacctggaa aacatgcca gccctgacc cacctctac 
ttgggcaaa aataaaata tcaaaaata aaaaacttg 
gacagtctg accaacgtg acgtgtctg agcctgcaa 
agcaaccga ataacgcgt atcgagaat ttggataag 
ctcacacaa ttgaccgaa ctgtacatt tccgagaac 
ggaattcat aaaatagaa aatcttacg cataacacg 
ttgctacag accttggat ttggcgaaa aataaaata 
aagttgatt gaaaacatc gagcatttg aacgacttg 
gaggagttt tggatgaat gataatgaa gtttccgat 
tggggaact gtcgagaat ttatcgaag aacaaaaag 
ctggctaca gtttacttg gagcggaat ccgctcgag 
aaggacccg atgtacagg aggaaattg aagatgatc 
gcgccctct ctaacccag atcgacgcg actttgtgt 
cagttgtaa aatattgtt ttgtaaatt aaggaaacc 
accgttgcc atctgtgca tttatgagt tgattagct 
aatgagtaa cgattaatt ttaggcgtc taattataa 
accaatcac ccaattgtg aaaatcggt tttaatttt 
gataagccc ctgctacgt aaaaataaa ccaaataac 
aataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY