Status
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Gene information
General information
TC034052
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005777 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7851008..7856835
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034052
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07480

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    previtellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 2

    previtellogenic egg chamber potentially number increased - Number of animals showing the phenotype: 2
    egg chamber stage sequence irregular - Number of animals showing the phenotype: 2

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 2

    tropharium structure irregular - Number of animals showing the phenotype: 2
    mature egg not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034052 RA
      MELRVGNKY RLGRKIGSG SFGDIYLGT NISTGEEVA 
IKLECIKTR HPQLHIESK FYKMMQGGV GIPQMKWCG 
SEGDYNVMV MELLGPSLE DLFNFCSRR FSLKTVLLL 
ADQLISRTD FIHSRNFIH RDIKPDNFL MGLGKKGNL 
VYIIDFGLA KKYRDGRTH QHIPYRENK NLTGTARYA 
SINTHLGIE QSRRDDLES LGYVLMYFN KGSLPWQGL 
KAATKRQKY ERISEKKMS TSIEELCKG FPMEFPTYL 
NYCRQLRFE ERPDYSYLR QLFRSLFHR QGFTYDYVF 
DWNMLKFGG SRGSHCEEN TGRSGTRYG SHAATPGDS 
TPTAPQSRT RRPHDRMDI VPANSAAPN PLEDVISSN 
SPRLYDSHE RRVSMRLRQ GAPNASTSD LSNSKQANA 
SGVAMAPPN QTAGQAGPP RRGSASKDP PRLKK
    CDS sequence
    • >TC034052 RA
      atggagctt agagtgggc aataagtac agactaggg 
cgcaagata ggctccggg tccttcggg gacatatat 
ttaggtacc aatatttcc acgggggag gaggtcgca 
attaagcta gaatgcatc aaaacgagg cacccgcag 
ctccacata gagtcgaaa ttttacaaa atgatgcag 
gggggagtg gggatcccc caaatgaaa tggtgcggc 
tcggaaggt gactacaac gtcatggtg atggagctc 
ctcggacca tccttagaa gacttattt aatttttgt 
tcacgtcgt ttctccctg aaaacagtt ctcctccta 
gccgaccaa ttaatatca cgtactgac ttcatccac 
tcgcggaac ttcatccac cgcgacatc aaacccgac 
aacttcctc atgggtttg ggcaaaaag ggtaactta 
gtttatata atcgacttt gggctagcg aaaaaatac 
agggacgga cggacccac cagcacata ccctaccga 
gaaaacaaa aatctaaca ggtacggcg cgatatgcg 
agcataaac acgcattta gggatagaa cagtcacga 
agggacgat ttggagtcg ttaggttac gttttaatg 
tatttcaat aagggctcg ctgccgtgg cagggcttg 
aaagcggcc acaaagagg caaaagtac gagaggatc 
tcggagaag aagatgtcc acgtccata gaagaattg 
tgcaaaggc tttccgatg gagttcccc acctacttg 
aactactgc agacagttg aggtttgag gaacggccc 
gattatagc tacttgaga cagctcttc cgttcactt 
ttccatcgc caaggtttc acctacgat tacgtattt 
gactggaat atgctcaaa tttgggggc tcgcggggc 
agccactgc gaggagaac accggccgt tcgggcacc 
cgttacggt agccacgcg gctaccccc ggggactcc 
actcccaca gccccccag agccgcacc cggcgcccc 
cacgaccgc atggacata gtaccggcc aacagcgcc 
gcccccaac cctctcgaa gacgtcatc agtagcaac 
tcgccccgt ttgtatgac agccacgaa cggcgcgtg 
tcaatgcga ctccgtcag ggggccccc aatgcctcc 
acctcagat ttgagcaat tcgaagcaa gcaaacgcc 
tccggagta gcaatggcg ccccccaac cagacggcg 
ggacaagca ggcccgcct agaagagga agcgcctcc 
aaagaccca ccaagactt aaaaagtga
    mRNA sequence
    • >TC034052 RA
      gagcccagt ggcgcaagc tggccgtct atttctcag 
ttgtttcgt gtggtgtgt caccaaaag gccataatg 
aaacgcgaa attgcaaaa ttaatcgtt cttgttttg 
cgtttttga cgaatctgt gataattgt gaaattcta 
aacacccca accgctagc tgtaaattc cctaaaaat 
aaacaaacg aatttctaa gccttctag ttacgtcaa 
atttcctat gatagttgg ctaatatct atggctaat 
atggccgcc tgaagtacg acagcattt catgtcttt 
tgttgtgtg aagtgaggt ggaatggag cttagagtg 
ggcaataag tacagacta gggcgcaag ataggctcc 
gggtccttc ggggacata tatttaggt accaatatt 
tccacgggg gaggaggtc gcaattaag ctagaatgc 
atcaaaacg aggcacccg cagctccac atagagtcg 
aaattttac aaaatgatg cagggggga gtggggatc 
ccccaaatg aaatggtgc ggctcggaa ggtgactac 
aacgtcatg gtgatggag ctcctcgga ccatcctta 
gaagactta tttaatttt tgttcacgt cgtttctcc 
ctgaaaaca gttctcctc ctagccgac caattaata 
tcacgtact gacttcatc cactcgcgg aacttcatc 
caccgcgac atcaaaccc gacaacttc ctcatgggt 
ttgggcaaa aagggtaac ttagtttat ataatcgac 
tttgggcta gcgaaaaaa tacagggac ggacggacc 
caccagcac ataccctac cgagaaaac aaaaatcta 
acaggtacg gcgcgatat gcgagcata aacacgcat 
ttagggata gaacagtca cgaagggac gatttggag 
tcgttaggt tacgtttta atgtatttc aataagggc 
tcgctgccg tggcagggc ttgaaagcg gccacaaag 
aggcaaaag tacgagagg atctcggag aagaagatg 
tccacgtcc atagaagaa ttgtgcaaa ggctttccg 
atggagttc cccacctac ttgaactac tgcagacag 
ttgaggttt gaggaacgg cccgattat agctacttg 
agacagctc ttccgttca cttttccat cgccaaggt 
ttcacctac gattacgta tttgactgg aatatgctc 
aaatttggg ggctcgcgg ggcagccac tgcgaggag 
aacaccggc cgttcgggc acccgttac ggtagccac 
gcggctacc cccggggac tccactccc acagccccc 
cagagccgc acccggcgc ccccacgac cgcatggac 
atagtaccg gccaacagc gccgccccc aaccctctc 
gaagacgtc atcagtagc aactcgccc cgtttgtat 
gacagccac gaacggcgc gtgtcaatg cgactccgt 
cagggggcc cccaatgcc tccacctca gatttgagc 
aattcgaag caagcaaac gcctccgga gtagcaatg 
gcgcccccc aaccagacg gcgggacaa gcaggcccg 
cctagaaga ggaagcgcc tccaaagac ccaccaaga 
cttaaaaag tgaaacagt ggtgaagga gaagaggtt 
tgagatgtg tgattttgt taagtttcg taactttga 
tttcgtatg tgcaatgga attaatttc aactcgttt 
tgtgataat tttttacac tttttgtcg tttgttttg 
agatgagag ttgagggaa ttgaagtta cggttggtt 
ttttcaatt agaagaaga gacaaatta actgaaaca 
aattgaaac gaaatctgt tttacctgc gggactttt 
gtacatatc tagactgat caactattg tgtattttg 
ttgtataat agcataagt gtttaggtt tcttttgtg 
accagtttg tataagagg aaacaatta attaattag 
ttggtgctt tggcattga ctcaatttt gtaacaaaa 
acattatca caagatatt tgttgatta gttgtgatt 
gtttttcgt gacagtcgt tgtctcatt gtgttctaa 
ttgactgca tttcaatgt tttgttcct atggaaaaa 
ttaagttta taatttata caaatattg taaaataat 
cacaaaaaa actagtaat ttaatgtaa ttttgatac 
aattaaaat taaccgcaa tttaggttt ttctaattc 
atttctaat attgtatgc tagatttac gcgattatt 
ctgtaaata agagatggt cttaagtgt tgtacatta 
taaatgaaa aataacctc agaatagtg cattatttt 
gtaaatttt gctggtaaa gaatctttt taagtactt 
agacttgaa aattgaacc acagttgtg tatttactg 
ttagcatta ccacagcat aatgttaca tgttttttt 
gttttcaag tgtcaaaaa acaaaaatc attattttt 
gttttattt gctgcacta tgtatgtta attacgaaa 
taaaagtca ctaaagttg
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