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Gene information
General information
TC030453 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 735671..738195
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030453
    iBeetle screen iB_07476

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030453 RA
      MNNQKIQIS NMTRKVLRY SLLWPKTNE ELNPGIEYQ 
      FSVLGFFLV TGVLVLCIT IRFFITIKA VHEVDAEVL 
      AILIASYGS YYMICAHLK NQHKVALLM RDLSVFNNF 
      GKPPNFDKR NNQLNFVAK LLALYSFLA TIFYNGEQL 
      INKTECKRI NKEKGLSDH YCGLLAPCW LPFEIDYFP 
      VFHLILIYA FTSGYLLIK MAIHISYNA FEIVSNIVL 
      RIEHLKAMI LETFENRNK QVCHKKFLQ CILYHIEIL 
      DFAARLDDS FFNSMFGHL ALTGGICAC LEKQIVSGV 
      NVVAGTLHF IGWILALFI GCVAGQYLI NASEILPSA 
      IWTAKWYDA DLELKKKVL FMLARSQKS LFIRAGPFG 
      ILCYPLFVT VLKTSYSIL CMLTS
    CDS sequence
    • >TC030453 RA
      atgaacaat caaaaaatc caaatttct aacatgacc 
      cgaaaagtt ttaagatac agtcttcta tggcctaag 
      acaaacgaa gaattaaat ccaggaatt gaataccaa 
      ttctctgtc cttggattt tttctagta actggtgtg 
      ttagtttta tgtataaca attcgtttt tttataaca 
      attaaagct gttcatgaa gtggatgcg gaagttctt 
      gcgattctc attgctagt tatggctcc tattacatg 
      atatgtgct catttgaaa aatcaacat aaagtggca 
      ctcttgatg cgagacctc tcagttttc aacaatttt 
      ggaaaacct ccaaatttt gataaacgt aacaaccaa 
      ctgaatttt gtagcaaaa cttttggcc ttgtattca 
      tttcttgcc acaattttc tacaacggt gaacaactt 
      ataaacaag acggaatgt aagagaatt aacaaggag 
      aaaggatta agtgatcat tattgtggt ttacttgcc 
      ccatgttgg ttaccattt gagattgat tatttccca 
      gtgtttcat ttgatttta atttatgct tttaccagt 
      ggttatcta ttaattaag atggcaata cacatttct 
      tacaacgct tttgaaatt gtaagcaac attgtgttg 
      agaattgaa cacttaaaa gccatgatt ttggaaact 
      ttcgaaaat cgaaacaaa caagtttgc cacaaaaaa 
      ttcctacaa tgtatattg tatcacatc gaaattttg 
      gattttgca gcaagactt gatgacagt ttttttaat 
      tcaatgttt ggtcacttg gctttgact ggaggtatt 
      tgcgcttgt ttggagaaa caaattgtt tcgggagtt 
      aatgttgtt gcaggcact ttgcatttt attggctgg 
      attcttgcc ttatttatc ggttgtgtt gctggacag 
      tatcttatt aatgcaagc gaaatttta ccaagtgct 
      atttggacc gcaaaatgg tatgatgca gacttggag 
      ttgaaaaaa aaagtgctt tttatgctt gcaagatcg 
      caaaaatcg ctttttatc agagctgga ccttttgga 
      attctttgt tatccactt tttgttacg gttttaaaa 
      acttcatac tctatttta tgtatgcta acgtcttaa
    mRNA sequence
    • >TC030453 RA
      aatcaaaaa ccaatatca cctttatca ccatttaaa 
      ctttaattt caactttga aagcgttgt taaaattta 
      ctgtgactt aaacagtaa taactaatt atgctttat 
      caaaagtga taaagtgta atatttgag taaaacaca 
      tcatacaat gaacaatca aaaaatcca aatttctaa 
      catgacccg aaaagtttt aagatacag tcttctatg 
      gcctaagac aaacgaaga attaaatcc aggaattga 
      ataccaatt ctctgtcct tggattttt tctagtaac 
      tggtgtgtt agttttatg tataacaat tcgtttttt 
      tataacaat taaagctgt tcatgaagt ggatgcgga 
      agttcttgc gattctcat tgctagtta tggctccta 
      ttacatgat atgtgctca tttgaaaaa tcaacataa 
      agtggcact cttgatgcg agacctctc agttttcaa 
      caattttgg aaaacctcc aaattttga taaacgtaa 
      caaccaact gaattttgt agcaaaact tttggcctt 
      gtattcatt tcttgccac aattttcta caacggtga 
      acaacttat aaacaagac ggaatgtaa gagaattaa 
      caaggagaa aggattaag tgatcatta ttgtggttt 
      acttgcccc atgttggtt accatttga gattgatta 
      tttcccagt gtttcattt gattttaat ttatgcttt 
      taccagtgg ttatctatt aattaagat ggcaataca 
      catttctta caacgcttt tgaaattgt aagcaacat 
      tgtgttgag aattgaaca cttaaaagc catgatttt 
      ggaaacttt cgaaaatcg aaacaaaca agtttgcca 
      caaaaaatt cctacaatg tatattgta tcacatcga 
      aattttgga ttttgcagc aagacttga tgacagttt 
      ttttaattc aatgtttgg tcacttggc tttgactgg 
      aggtatttg cgcttgttt ggagaaaca aattgtttc 
      gggagttaa tgttgttgc aggcacttt gcattttat 
      tggctggat tcttgcctt atttatcgg ttgtgttgc 
      tggacagta tcttattaa tgcaagcga aattttacc 
      aagtgctat ttggaccgc aaaatggta tgatgcaga 
      cttggagtt gaaaaaaaa agtgctttt tatgcttgc 
      aagatcgca aaaatcgct ttttatcag agctggacc 
      ttttggaat tctttgtta tccactttt tgttacggt 
      tttaaaaac ttcatactc tattttatg tatgctaac 
      gtcttaaaa ttaattcgt aattagcac ttttaaact 
      ctataatat aatgctaat taagtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY