Status
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Gene information
General information
TC031444
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030364,TC011015,TC011017,TC030363
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6616079..6631646
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031444
    iBeetle screen iB_07474

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031444 RA
      MSELEKQLP YYFLMQFCI NFFYSKTVK VVTSSCIII 
      QSLSLLLQV YFIITNFSK ELILKYGCE MSLATYLLT 
      SLLVDVVVE NTTKQLISE GHTSFWSID SCGHDVKNH 
      IIANSARLS VVIYFILAW FAVLGISVL PVWGDQSEW 
      ILFVQIFNT WKKILCYVY LSTLAVMVF LSIRLPAML 
      LYGILQIHV QIILINQRI IQIGRENTN DIRMMNQMS 
      YQNRIYKEL GFCVSQHAR IKSLSTLLE IFYICQNFS 
      KELVFQYGC ITSLATYVI TSMTTGFII ENDAKNLIR 
      ETVTAFWPI DFCGPQVEQ LIFKRVARI NTFNFFLLA 
      WFAIFGIIM FPVWGDESE WMLCVIAFK KYFPKWWRV 
      PYYVFFATY PMVAYSAIR IPAMLLYGI LQINMQFFL 
      ISQKIIQIS QKPQNKTHQ PGFYQKTVY KKLCQCISQ 
      HAEIKRWLQ RFLKMVKSV MPVFIFVGG LCFMSILFF 
      VVYTFQSTS NILKVRLGV ILMICNLIL VTFAQAGQT 
      VIDEITISM VVELLLEKD TNNLVCEAR SLFWTIDSC 
      GVQAQQIIH KRAVVMNAT FGFILMWVA TLGVIMFPI 
      WGDQSEWVL CVKIFENYF ENWSQMANF VFFSTFPMV 
      AYSTIRLPA MLLYGILQT HMQIFLINQ KITEISRSK 
      DQEKIYKEL CLCVSHHVE IKRWLQRFL KMVQLTMLM 
      LIPLGVLSC VCVLFFVIY VRQVLEGFP YYYLLQLCL 
      DVGYSKMMK IANIFCIII NLLNVLAQI GYIKQNFGK 
      ELLLRYACG IQLTIYTIV TMLFEFLVE QNVKKLMDE 
      ALSEMWPID FCGLEIKKL ILKRSTVMN SIFYFMFAW 
      FAILAIVML PMWGDQSEW LLYDRICKE FFATWWKIP 
      YYFYFTTFP VVAFSGIRL PGLLLYTIL QTHMQIILI 
      NQKLVQISG GLDGINDVR MIDQKNYQK RIYKGLRLC 
      VAHHVAIKR WLQKPVKIV QSLMPIYII MGSTIFISL 
      LFATVYSFR DSSNILKVR MSVVLMICC LILCMGAEA 
      GQALSNETS RVFDTLVNC PWHLWDQKN KKALTIFLP 
      NTLQPVTIT LAGITLNYS FAVGLLKSS ASYALVLYN 
      MRN
    CDS sequence
    • >TC031444 RA
      atgtccgaa ctcgagaaa caactcccg tactatttt 
      ctaatgcaa ttctgtata aacttcttc tactcaaaa 
      accgtaaaa gtggtcaca tccagctgt atcataatc 
      cagtcacta agtttactc ctacaagtg tacttcatc 
      atcaccaac tttagcaaa gaactaatt ctaaaatat 
      ggctgtgaa atgtcgctt gcaacttat ctactaaca 
      tcactactg gtggacgtt gtggtcgaa aacaccaca 
      aaacaatta ataagtgag ggacacact tcgttctgg 
      tccattgat tcctgcggt catgacgtg aaaaaccac 
      attatcgca aactcggca cgcctcagt gttgtcatt 
      tacttcatt ttagcgtgg tttgctgtt ttggggatt 
      tcggtgctt ccagtgtgg ggcgatcag agcgaatgg 
      attttgttc gtgcaaatt ttcaacact tggaaaaaa 
      attttatgt tacgtttac ttatcaact cttgcagtt 
      atggttttt ttgagtatt cggctcccg gccatgctg 
      ttgtacggt attttgcaa attcacgtc caaattatt 
      ttaatcaat cagaggatc attcaaatt gggcgagag 
      aacacaaat gatattaga atgatgaac caaatgagt 
      taccagaac aggatttac aaagagttg ggtttttgt 
      gtttcacaa cacgctaga attaaaagc ttgagcacc 
      ttactggaa attttctac atttgccaa aatttcagc 
      aaggaactt gtgttccaa tacgggtgc ataacctca 
      ctagcaact tacgtgata acgtcaatg accaccggg 
      tttataatc gaaaacgac gccaaaaac ttaatccgt 
      gaaactgtg accgcattc tggcccata gatttctgc 
      ggcccacaa gtcgagcaa ttaattttc aaacgcgtg 
      gcacgaatt aacactttt aattttttt ctactagcc 
      tggtttgca atttttgga attatcatg tttccggtt 
      tggggcgac gaaagcgag tggatgttg tgtgtcatt 
      gcttttaaa aaatacttc ccgaaatgg tggagagtt 
      ccatactat gtttttttt gcaacttat ccaatggtc 
      gcttattcg gccatccgg atacccgct atgctcttg 
      tacggaatt ttgcaaatc aacatgcaa tttttccta 
      atcagtcag aaaatcatc caaatttca caaaaaccc 
      caaaataaa acacaccag cccgggttt tatcaaaaa 
      actgtttac aagaaactc tgtcagtgt atttcccaa 
      cacgctgaa attaaacga tggcttcag aggtttctg 
      aaaatggtg aaatcggtg atgccggtg tttattttc 
      gtcgggggt ttgtgtttc atgagtatt ttatttttc 
      gtggtttat acgtttcaa agtacaagt aacatcctt 
      aaagtacgt ttgggggtt attttaatg atctgtaac 
      ctgattttg gttactttt gctcaagct ggacaaaca 
      gtcattgat gagataacg atatcgatg gtcgttgag 
      ttgttgctc gaaaaagac acaaataat ctagtatgc 
      gaggcacgt tccctattc tggacgatt gactcctgt 
      ggcgtccaa gcccagcaa ataattcac aaacgtgca 
      gttgtaatg aatgccact tttggattt attttaatg 
      tgggttgcc actttgggc gttataatg ttcccgatt 
      tggggcgac cagagcgaa tgggtttta tgtgtgaaa 
      atttttgaa aactatttc gaaaactgg agccaaatg 
      gcgaatttt gtctttttt tccacgttt ccgatggtc 
      gcttattcg accattcgg ctccctgcc atgctgttg 
      tacgggatt ttacaaaca cacatgcag atttttctg 
      atcaatcag aaaatcacc gaaatttcg cggtcaaaa 
      gaccaggaa aaaatctac aaagagttg tgtctttgt 
      gtctctcat cacgttgaa atcaagagg tggcttcag 
      aggtttctc aaaatggtg cagttgacg atgctgatg 
      ctaattcct ttgggagtt ttaagttgc gtttgtgtc 
      ctatttttt gttatctac gtaagacaa gtgttggaa 
      ggcttcccc tattattac ctcttacaa ctgtgtctg 
      gacgtcggc tattccaaa atgatgaaa attgcaaac 
      attttttgt ataataatt aatttattg aatgtctta 
      gcgcaaata ggttacatc aagcagaac tttggcaag 
      gagctgctc ctgaggtac gcatgcggg atacaatta 
      acaatttac acaatcgta acaatgttg tttgaattt 
      ttggtcgaa caaaacgta aaaaaacta atggacgag 
      gcactttcc gaaatgtgg ccaattgat ttctgtggt 
      ttggaaatc aaaaaactg atcctcaaa cgctccact 
      gttatgaac tcaattttt tacttcatg tttgcttgg 
      tttgccatt ctggcaatt gtgatgctc ccaatgtgg 
      ggcgatcag agtgaatgg ttgttgtac gaccgaatt 
      tgtaaagaa tttttcgca acgtggtgg aaaatcccc 
      tattatttt tacttcacc actttccca gtggttgct 
      ttttcgggc atccgacta ccagggctg ttattgtac 
      actatttta caaacacat atgcaaatt attttaatt 
      aaccaaaaa ctggtccag atttctggc ggtttggac 
      ggtattaac gatgttaga atgattgac caaaaaaac 
      taccagaaa agaatttac aaagggttg cggctgtgt 
      gttgcacac catgtggct attaaaagg tggctccaa 
      aaaccagtt aaaatcgta cagtcacta atgccgatt 
      tatattatc atggggtca acaattttt atatcattg 
      ttatttgca acagtctat agttttcgc gattctagc 
      aacattctt aaagttcgt atgagtgtt gttttaatg 
      atttgttgc cttattttg tgcatgggt gctgaagct 
      gggcaagca ctcagcaat gaaactagt cgtgttttt 
      gatacgtta gtaaactgt ccgtggcat ctgtgggac 
      caaaaaaat aaaaaagcc ctaacaatt tttctgccg 
      aatacttta caacctgtt acaatcaca ttggccggt 
      ataacgcta aactatagt ttcgcagtt ggattgctg 
      aaaagcagt gcctcatat gccttagtg ctttacaat 
      atgagaaac taa
    mRNA sequence
    • >TC031444 RA
      tgtcagtct gttttaagc aacgcccga aataaacat 
      gtccgaact cgagaaaca actcccgta ctattttct 
      aatgcaatt ctgtataaa cttcttcta ctcaaaaac 
      cgtaaaagt ggtcacatc cagctgtat cataatcca 
      gtcactaag tttactcct acaagtgta cttcatcat 
      caccaactt tagcaaaga actaattct aaaatatgg 
      ctgtgaaat gtcgcttgc aacttatct actaacatc 
      actactggt ggacgttgt ggtcgaaaa caccacaaa 
      acaattaat aagtgaggg acacacttc gttctggtc 
      cattgattc ctgcggtca tgacgtgaa aaaccacat 
      tatcgcaaa ctcggcacg cctcagtgt tgtcattta 
      cttcatttt agcgtggtt tgctgtttt ggggatttc 
      ggtgcttcc agtgtgggg cgatcagag cgaatggat 
      tttgttcgt gcaaatttt caacacttg gaaaaaaat 
      tttatgtta cgtttactt atcaactct tgcagttat 
      ggttttttt gagtattcg gctcccggc catgctgtt 
      gtacggtat tttgcaaat tcacgtcca aattatttt 
      aatcaatca gaggatcat tcaaattgg gcgagagaa 
      cacaaatga tattagaat gatgaacca aatgagtta 
      ccagaacag gatttacaa agagttggg tttttgtgt 
      ttcacaaca cgctagaat taaaagctt gagcacctt 
      actggaaat tttctacat ttgccaaaa tttcagcaa 
      ggaacttgt gttccaata cgggtgcat aacctcact 
      agcaactta cgtgataac gtcaatgac caccgggtt 
      tataatcga aaacgacgc caaaaactt aatccgtga 
      aactgtgac cgcattctg gcccataga tttctgcgg 
      cccacaagt cgagcaatt aattttcaa acgcgtggc 
      acgaattaa cacttttaa tttttttct actagcctg 
      gtttgcaat ttttggaat tatcatgtt tccggtttg 
      gggcgacga aagcgagtg gatgttgtg tgtcattgc 
      ttttaaaaa atacttccc gaaatggtg gagagttcc 
      atactatgt tttttttgc aacttatcc aatggtcgc 
      ttattcggc catccggat acccgctat gctcttgta 
      cggaatttt gcaaatcaa catgcaatt tttcctaat 
      cagtcagaa aatcatcca aatttcaca aaaacccca 
      aaataaaac acaccagcc cgggtttta tcaaaaaac 
      tgtttacaa gaaactctg tcagtgtat ttcccaaca 
      cgctgaaat taaacgatg gcttcagag gtttctgaa 
      aatggtgaa atcggtgat gccggtgtt tattttcgt 
      cgggggttt gtgtttcat gagtatttt atttttcgt 
      ggtttatac gtttcaaag tacaagtaa catccttaa 
      agtacgttt gggggttat tttaatgat ctgtaacct 
      gattttggt tacttttgc tcaagctgg acaaacagt 
      cattgatga gataacgat atcgatggt cgttgagtt 
      gttgctcga aaaagacac aaataatct agtatgcga 
      ggcacgttc cctattctg gacgattga ctcctgtgg 
      cgtccaagc ccagcaaat aattcacaa acgtgcagt 
      tgtaatgaa tgccacttt tggatttat tttaatgtg 
      ggttgccac tttgggcgt tataatgtt cccgatttg 
      gggcgacca gagcgaatg ggttttatg tgtgaaaat 
      ttttgaaaa ctatttcga aaactggag ccaaatggc 
      gaattttgt ctttttttc cacgtttcc gatggtcgc 
      ttattcgac cattcggct ccctgccat gctgttgta 
      cgggatttt acaaacaca catgcagat ttttctgat 
      caatcagaa aatcaccga aatttcgcg gtcaaaaga 
      ccaggaaaa aatctacaa agagttgtg tctttgtgt 
      ctctcatca cgttgaaat caagaggtg gcttcagag 
      gtttctcaa aatggtgca gttgacgat gctgatgct 
      aattccttt gggagtttt aagttgcgt ttgtgtcct 
      attttttgt tatctacgt aagacaagt gttggaagg 
      cttccccta ttattacct cttacaact gtgtctgga 
      cgtcggcta ttccaaaat gatgaaaat tgcaaacat 
      tttttgtat aataattaa tttattgaa tgtcttagc 
      gcaaatagg ttacatcaa gcagaactt tggcaagga 
      gctgctcct gaggtacgc atgcgggat acaattaac 
      aatttacac aatcgtaac aatgttgtt tgaattttt 
      ggtcgaaca aaacgtaaa aaaactaat ggacgaggc 
      actttccga aatgtggcc aattgattt ctgtggttt 
      ggaaatcaa aaaactgat cctcaaacg ctccactgt 
      tatgaactc aatttttta cttcatgtt tgcttggtt 
      tgccattct ggcaattgt gatgctccc aatgtgggg 
      cgatcagag tgaatggtt gttgtacga ccgaatttg 
      taaagaatt tttcgcaac gtggtggaa aatccccta 
      ttattttta cttcaccac tttcccagt ggttgcttt 
      ttcgggcat ccgactacc agggctgtt attgtacac 
      tattttaca aacacatat gcaaattat tttaattaa 
      ccaaaaact ggtccagat ttctggcgg tttggacgg 
      tattaacga tgttagaat gattgacca aaaaaacta 
      ccagaaaag aatttacaa agggttgcg gctgtgtgt 
      tgcacacca tgtggctat taaaaggtg gctccaaaa 
      accagttaa aatcgtaca gtcactaat gccgattta 
      tattatcat ggggtcaac aatttttat atcattgtt 
      atttgcaac agtctatag ttttcgcga ttctagcaa 
      cattcttaa agttcgtat gagtgttgt tttaatgat 
      ttgttgcct tattttgtg catgggtgc tgaagctgg 
      gcaagcact cagcaatga aactagtcg tgtttttga 
      tacgttagt aaactgtcc gtggcatct gtgggacca 
      aaaaaataa aaaagccct aacaatttt tctgccgaa 
      tactttaca acctgttac aatcacatt ggccggtat 
      aacgctaaa ctatagttt cgcagttgg attgctgaa 
      aagcagtgc ctcatatgc cttagtgct ttacaatat 
      gagaaacta atatcgatg taataaaat gtgcactaa 
      ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY