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Gene information
General information
TC030358 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17629951..17631401
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030358
    iBeetle screen iB_07473

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030358 RA
      MAKARYLQD SDDPFIFIR KLFVDYGYS RIINSYNRF 
      IFIFHTCSL LLEGYYIIK NFSIDFFTQ YGGATNLIL 
      YFLVTQFIV IIKQDFVHQ IVEESKSLY WSMDFLDVN 
      KQAQILQEM TKLKRKIYV LWMSTVIFA IMMLPVWGD 
      YSEAHLFPQ IYQTYFGNW SPIFYYFYV STFPFAAYT 
      GLRIAAMAL YFTLIIHFQ IILLNQKIL QIAQERDTS 
      QEEIAKNLR SCVCQHVTL KRFVAKVLK SIENAVPVY 
      FCLAILCLI TVLFVILNN LDTSTSNHL KARFLFAGI 
      YGTVILYTF TEAGQLLSE INDQVFNTL MQCPWYNWN 
      TKNKKTLMI FMLGSLKPV QIVWGGVNV DYKFGGSVI 
      QTCCSYALV LYQMRNAK
    CDS sequence
    • >TC030358 RA
      atggcaaaa gcaagatat ttacaagat tccgacgac 
      cctttcatt tttataagg aaattgttt gtcgattat 
      ggctacagc aggatcatt aacagttac aatagattt 
      attttcatt ttccatact tgtagttta ttgctagaa 
      ggctactac ataatcaaa aatttcagc atagatttt 
      ttcacgcaa tacggagga gcaacgaac ttaatttta 
      tattttcta gtcacacaa tttattgtc attatcaaa 
      caggatttt gttcaccaa atagtggaa gaaagcaaa 
      tcactttac tggtctatg gattttctc gatgtcaac 
      aaacaagcg caaattttg caagaaatg acaaaactt 
      aagcgaaaa atttatgtg ttgtggatg tcgactgtg 
      atttttgct attatgatg ctgccggtg tggggagat 
      tacagtgag gcacattta ttcccacaa atttaccag 
      acctatttc gggaattgg tcgccaatt ttttattat 
      ttttacgta tccactttc ccttttgca gcctataca 
      ggtcttcga atcgctgct atggcatta tactttact 
      ttgataata cacttccaa ataatccta ctaaatcaa 
      aaaatcttg caaattgcg caagaacgt gacacgagt 
      caagaagaa attgctaag aatttgcgt tcatgtgtg 
      tgtcaacac gtcacatta aaaagattt gttgccaaa 
      gttttaaaa tccatagaa aatgcggtt cctgtttat 
      ttctgtttg gcaatattg tgtctgatt acagtatta 
      tttgttatt ctgaataat ttggataca agtacaagc 
      aaccatcta aaagcccgt tttcttttt gctggaatt 
      tatggtact gtcattttg tacacattt acagaagct 
      ggtcaactt cttagcgaa attaatgat caagttttt 
      aatacttta atgcagtgt ccttggtac aactggaat 
      actaaaaat aaaaaaact ctgatgatt tttatgcta 
      ggctcgcta aaaccggtg caaattgtt tggggtggt 
      gtcaacgta gattacaaa tttggaggc tctgttatt 
      cagacttgt tgctcttac gctttggtt ttgtatcaa 
      atgagaaat gcaaagtga
    mRNA sequence
    • >TC030358 RA
      atcgagaag atagctaag tctataatt tgaaatttt 
      cgaaatcaa tggcaaaag caagatatt tacaagatt 
      ccgacgacc ctttcattt ttataagga aattgtttg 
      tcgattatg gctacagca ggatcatta acagttaca 
      atagattta ttttcattt tccatactt gtagtttat 
      tgctagaag gctactaca taatcaaaa atttcagca 
      tagattttt tcacgcaat acggaggag caacgaact 
      taattttat attttctag tcacacaat ttattgtca 
      ttatcaaac aggattttg ttcaccaaa tagtggaag 
      aaagcaaat cactttact ggtctatgg attttctcg 
      atgtcaaca aacaagcgc aaattttgc aagaaatga 
      caaaactta agcgaaaaa tttatgtgt tgtggatgt 
      cgactgtga tttttgcta ttatgatgc tgccggtgt 
      ggggagatt acagtgagg cacatttat tcccacaaa 
      tttaccaga cctatttcg ggaattggt cgccaattt 
      tttattatt tttacgtat ccactttcc cttttgcag 
      cctatacag gtcttcgaa tcgctgcta tggcattat 
      actttactt tgataatac acttccaaa taatcctac 
      taaatcaaa aaatcttgc aaattgcgc aagaacgtg 
      acacgagtc aagaagaaa ttgctaaga atttgcgtt 
      catgtgtgt gtcaacacg tcacattaa aaagatttg 
      ttgccaaag ttttaaaat ccatagaaa atgcggttc 
      ctgtttatt tctgtttgg caatattgt gtctgatta 
      cagtattat ttgttattc tgaataatt tggatacaa 
      gtacaagca accatctaa aagcccgtt ttctttttg 
      ctggaattt atggtactg tcattttgt acacattta 
      cagaagctg gtcaacttc ttagcgaaa ttaatgatc 
      aagttttta atactttaa tgcagtgtc cttggtaca 
      actggaata ctaaaaata aaaaaactc tgatgattt 
      ttatgctag gctcgctaa aaccggtgc aaattgttt 
      ggggtggtg tcaacgtag attacaaat ttggaggct 
      ctgttattc agacttgtt gctcttacg ctttggttt 
      tgtatcaaa tgagaaatg caaagtgaa atgggaatt 
      taagaataa attgcgtgt ttctta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY