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Gene information
General information
TC030240 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14251813..14265361
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030240
    iBeetle screen iB_07457

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030240 RA
      MTRAMVRPE LDLNSLKCI TKVLTILGL LSCSFPKKK 
      LVYCIIFGA IATLTCIEG LRDCPRKYS TPLAKITTI 
      LQRYCSVLL VFLTYFFNI LFRKKLLNA VKILSKIDE 
      TLNSKPLKP VKIRTKVYL VYCSSALVL AAMTTFHLI 
      YEAHGFSYK CGIQYHICN TVISATVCF MMLLMLEIW 
      RRFTILNNY LTIVLGETW TSLSVYHLV EISEIHFDL 
      CQAADDLNR YFEVQVLTI FGVSFYFFI STFFYFFTT 
      GNIIATYDK QFYIHNALI VRALFLFFQ LWATVYTSS 
      QVTRESEKT AEIVHTTKR NTLNSSLRV WVELFSHQT 
      IFKRVNFTV LGLFPIEWN VIFGIVCAT TNYLIILIQ 
      FNDHILKLS
    CDS sequence
    • >TC030240 RA
      atgactaga gctatggtt cggccagag cttgactta 
      aattctctg aaatgcatc acaaaagta ttgacaatc 
      ttgggatta ctctcgtgt tcctttccc aagaaaaag 
      ctagtctat tgcattatt tttggcgca attgccact 
      ctaacatgt attgaaggg ttgcgagac tgcccccga 
      aaatactca acgcccttg gcaaaaatt acaacgatt 
      ttgcaaaga tactgctca gttttgttg gttttccta 
      acttacttt ttcaatatt ttgttccga aaaaaactt 
      ctcaacgcg gtaaaaatt ctatcaaaa atcgacgaa 
      acactgaac tccaagcct ctaaagcca gtaaaaatc 
      agaaccaaa gtgtatctt gtgtactgt tcaagtgcg 
      ctcgttcta gcggctatg actacgttt catttaatt 
      tacgaagct cacggtttc agttacaaa tgtgggata 
      cagtaccac atttgcaac actgtgatt agtgcaacc 
      gtgtgtttc atgatgctt ttgatgttg gaaatttgg 
      aggagattt acaattttg aataattac ttaacaatt 
      gttttggga gaaacctgg accagttta tcggtttat 
      cacctggtg gaaataagc gaaattcat tttgatttg 
      tgtcaagct gctgatgac ctaaaccgg tattttgag 
      gtccaggtt ttaacaatc tttggagtg tctttctat 
      ttcttcatt tcgactttt ttctatttt ttcaccacg 
      ggaaatata attgccacg tacgataaa cagttttac 
      atccacaat gctctaata gtcagagct ttatttctc 
      tttttccaa ctgtgggcc actgtttac acatcgagt 
      caagtcacg agagagtcg gaaaaaacg gccgaaatt 
      gtgcacact actaaaaga aacactttg aattcaagc 
      ttacgtgtt tgggtcgag ttgttttca catcaaacg 
      atattcaaa cgtgttaat tttacggtt ctgggcttg 
      tttccaatc gaatggaat gtaattttc gggatcgtg 
      tgtgcaact actaattat ttaattatc ttgatacaa 
      ttcaacgac cacatttta aagctttcg taa
    mRNA sequence
    • >TC030240 RA
      ggctttgac caaaataat tttctcaaa aaaaggaca 
      aaaaatttg tcactcgtt tttctctaa acctgttag 
      tttcggaga aaaatgcgc accctgtac acaaagttg 
      gattacatt ctaaaccac ttaatttca cactgctgt 
      cttacatta aatgttagt ttaatcgaa aaaaatctt 
      atttatatg tcgtaattg acgggttgt tgaatgttt 
      tgttaagac aagtgagga cgttaaaat aaagattta 
      atggtgtgt tatcgactt tggcagttg tccattatc 
      aatgagttt ctttcacat tccaagaga ttttataac 
      gcaaagctc agttttgta taaagggat agcgaattg 
      ttgaagttt tgttgtcta tttttatct agtcctatc 
      caaaagttt atttgttta gttattgtc gactcgatg 
      aattcaatg tatattgta aatagtgta aggatcaaa 
      aattgtgtt ttgtaatac aagtattta taattaatt 
      tataatatt tacaattat aatcgatta gagtagtcc 
      gtagcatgc cagcatgca cgatagagc agacactgt 
      gatgacaga ctgaacagc tttgaacca attttaata 
      actacctag actattcat tgattgaca atgtccaaa 
      gtattcaac caactaacc ttcggcttg aaatagaca 
      attcctcta ccgcttctc ccacagaaa gttgttcaa 
      attctgatt ttctgcccc agcatggaa ttttatgtg 
      acacgtttt aaactaact tgtaagatt gcaccgttt 
      tcctctacc actttgtaa atatagcct aattgtcgt 
      gtttctaaa ttctaaaaa attccatgc tcaaaacgt 
      attccatat aaaatgagc ggtttaatt tatatggaa 
      tacgagaat ttgttcttc aaattgcca caatgcttt 
      aagtgacct catctatca gtataatca gatttgcca 
      ttctgtacc gttagagcc aatattgtt agaactttg 
      gcttaattg cctttacta taatgcacc acgatcatt 
      taatctcgt tatgaacat gaagaaacc ctcaaatat 
      tttgtagct atatcgact cgacttact gtacatatt 
      tcctataat gtacatgca tcgaatttt aaattaaaa 
      cacaatgta tgttctaat tcgttacat tatacaata 
      aatattttc atctgcaac ggcgccttt catttccaa 
      acaactaac caattaact tacaacaac aaacaagcc 
      attcaatta tactccaca aaatggtgt ggtcaccca 
      aagatgtta agtacagtt gttatgact agagctatg 
      gttcggcca gagcttgac ttaaattct ctgaaatgc 
      atcacaaaa gtattgaca atcttggga ttactctcg 
      tgttccttt cccaagaaa aagctagtc tattgcatt 
      atttttggc gcaattgcc actctaaca tgtattgaa 
      gggttgcga gactgcccc cgaaaatac tcaacgccc 
      ttggcaaaa attacaacg attttgcaa agatactgc 
      tcagttttg ttggttttc ctaacttac tttttcaat 
      attttgttc cgaaaaaaa cttctcaac gcggtaaaa 
      attctatca aaaatcgac gaaacactg aactccaag 
      cctctaaag ccagtaaaa atcagaacc aaagtgtat 
      cttgtgtac tgttcaagt gcgctcgtt ctagcggct 
      atgactacg tttcattta atttacgaa gctcacggt 
      ttcagttac aaatgtggg atacagtac cacatttgc 
      aacactgtg attagtgca accgtgtgt ttcatgatg 
      cttttgatg ttggaaatt tggaggaga tttacaatt 
      ttgaataat tacttaaca attgttttg ggagaaacc 
      tggaccagt ttatcggtt tatcacctg gtggaaata 
      agcgaaatt cattttgat ttgtgtcaa gctgctgat 
      gacctaaac cggtatttt gaggtccag gttttaaca 
      atctttgga gtgtctttc tatttcttc atttcgact 
      tttttctat tttttcacc acgggaaat ataattgcc 
      acgtacgat aaacagttt tacatccac aatgctcta 
      atagtcaga gctttattt ctctttttc caactgtgg 
      gccactgtt tacacatcg agtcaagtc acgagagag 
      tcggaaaaa acggccgaa attgtgcac actactaaa 
      agaaacact ttgaattca agcttacgt gtttgggtc 
      gagttgttt tcacatcaa acgatattc aaacgtgtt 
      aattttacg gttctgggc ttgtttcca atcgaatgg 
      aatgtaatt ttcgggatc gtgtgtgca actactaat 
      tatttaatt atcttgata caattcaac gaccacatt 
      ttaaagctt tcgtaagta atcaatgac ggcggaaag 
      gactaaaca cgatgcata att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY