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Gene information
General information
TC030219 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 16132348..16133631
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC030219
      iBeetle screen iB_07453

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC030219 RA
        MTLLPTTPH YLKFFYQLT GFVQFSYNE SLESLLSRL 
        WCLPFYLYS LYLCFYFNS SDAYSGTGI FFYISIFSN 
        SVNVFSIFT TFWICYTRS TSLRQILSQ ISQIQVDVK 
        QTSENNWHT FCIIGLLMA NIATLLVVN TPKNFITYL 
        YMLPLVINC AINLLIRDV LNLVFCKFE SVNYQLGRQ 
        VTSKNLKEI FPSTKINRI EVLQDIEVD NDMKLVENL 
        SHIHYNLVK LSFMVCENF EITIIANLI LWFEKIIET 
        VYYAVYISI NETINNLVV DYVINLLFV AYSFYWLFT 
        LIGHFCRVE REANKTATI VHDIWNKVA VERKFDKRI 
        RHLQLVSVK LLNTKLKFT ARNFFTLDW TCCHMVKGK 
        I
      CDS sequence
      • >TC030219 RA
        atgactttg ctccctact acaccacat tacttgaaa 
        tttttttat caattaacc ggattcgtc caattttct 
        tataacgag tcactagaa tctctcctc tcacgtttg 
        tggtgtttg cccttctat ttgtattct ctttacctc 
        tgtttctac ttcaattcc tctgacgct tattccggg 
        actggaatt ttcttctac atcagtatt ttttccaac 
        agcgttaac gttttttca attttcaca actttctgg 
        atttgttac acgagaagt acaagtttg agacaaatc 
        ttgtctcag ataagtcaa atccaagtt gatgttaag 
        caaacgtcc gaaaataat tggcacacg ttttgtatt 
        attggttta ttaatggca aatattgcc actcttctt 
        gtcgttaat acaccgaag aatttcata acttatctt 
        tacatgttg ccgcttgtg ataaattgt gcaattaat 
        cttctgata agggatgtt ttaaatctg gttttttgc 
        aagtttgag tcagtcaat taccaactg ggaagacag 
        gtgacctca aaaaatttg aaagagatc tttccttca 
        accaaaata aaccggatt gaggttctt caagacatt 
        gaagttgac aatgatatg aaactcgtt gaaaacctt 
        tcgcacatt cactacaat ttagtgaaa ctgtcgttc 
        atggtttgt gaaaatttt gagattaca ataatagcg 
        aatttaatt ctatggttt gagaaaata atagaaacc 
        gtttattac gctgtttat atcagtatc aacgagaca 
        ataaacaat ctagttgtt gattatgtg atcaatttg 
        ttatttgtg gcttactca ttctattgg ctttttaca 
        ctcattgga cacttttgc cgcgtcgaa cgtgaagct 
        aacaaaact gcaaccatt gtgcatgat atttggaac 
        aaagttgcc gtcgaaagg aaatttgat aagagaata 
        agacattta cagttagtt tctgttaaa ttacttaat 
        acgaagcta aagtttact gcaagaaat ttctttact 
        ttggactgg acttgctgt cacatggta aaaggtaaa 
        atttaa
      mRNA sequence
      • >TC030219 RA
        gacgagtca tgtttattg tatctatta gttaatgga 
        aatatgtaa ggatcgatg caaacactc aattcaccc 
        taatttaga cgttgccat gactttgct ccctactac 
        accacatta cttgaaatt tttttatca attaaccgg 
        attcgtcca attttctta taacgagtc actagaatc 
        tctcctctc acgtttgtg gtgtttgcc cttctattt 
        gtattctct ttacctctg tttctactt caattcctc 
        tgacgctta ttccgggac tggaatttt cttctacat 
        cagtatttt ttccaacag cgttaacgt tttttcaat 
        tttcacaac tttctggat ttgttacac gagaagtac 
        aagtttgag acaaatctt gtctcagat aagtcaaat 
        ccaagttga tgttaagca aacgtccga aaataattg 
        gcacacgtt ttgtattat tggtttatt aatggcaaa 
        tattgccac tcttcttgt cgttaatac accgaagaa 
        tttcataac ttatcttta catgttgcc gcttgtgat 
        aaattgtgc aattaatct tctgataag ggatgtttt 
        aaatctggt tttttgcaa gtttgagtc agtcaatta 
        ccaactggg aagacaggt gacctcaaa aaatttgaa 
        agagatctt tccttcaac caaaataaa ccggattga 
        ggttcttca agacattga agttgacaa tgatatgaa 
        actcgttga aaacctttc gcacattca ctacaattt 
        agtgaaact gtcgttcat ggtttgtga aaattttga 
        gattacaat aatagcgaa tttaattct atggtttga 
        gaaaataat agaaaccgt ttattacgc tgtttatat 
        cagtatcaa cgagacaat aaacaatct agttgttga 
        ttatgtgat caatttgtt atttgtggc ttactcatt 
        ctattggct ttttacact cattggaca cttttgccg 
        cgtcgaacg tgaagctaa caaaactgc aaccattgt 
        gcatgatat ttggaacaa agttgccgt cgaaaggaa 
        atttgataa gagaataag acatttaca gttagtttc 
        tgttaaatt acttaatac gaagctaaa gtttactgc 
        aagaaattt ctttacttt ggactggac ttgctgtca 
        catggtaaa aggtaaaat ttaataatt tttttctgc 
        acactcttg tattttagg tcattgctg cggttacaa 
        cgtatgtta tcattctgg tacagttct gtagtcatg 
        actgaataa atttagttg ggaaat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY