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Gene information
General information
TC030128 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5148213..5151715
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030128
    iBeetle screen iB_07444

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030128 RA
      MTSSKPLLI NPNLFKTLR PLIKLSRIF GLCPITYKN 
      SDKCLNLRI KWSWSLYIL QLIAVTVIT SWAIWGFIR 
      DMQIAEFLN LGFTSAIDL YIAIFDVNE IIITGFIFV 
      AIIPSTFKH LPEIIDNFN KIDDTNPQL PTNKIHRKI 
      LFLLSFVLV YMTVAYTCD LIMWSQDLL VGFIHDLPY 
      YILYSVVVI HEVQYWFLV TLIRLRMSA MNGLVAETL 
      NRNTDISSQ EVCLLVETH SNILEAVST VNRCFSGPT 
      AIILLSCYL HLVVCPYIL YVVASSDNG LFCNTVYFL 
      WISVHFSRL LVIIEVCNN CENECHKTR NLIFKLLAN 
      TSKKGDVRR ELKLFAYKN VGKKVRFSS YAVPKINRK 
      LLISIATSI SSYWMILYQ FGASTTGL
    CDS sequence
    • >TC030128 RA
      atgaccagt agtaaacca ttgttaata aaccccaat 
      ctgtttaaa acgttgcga cctctaatc aaactatcg 
      cgtattttc ggcctgtgc cccataacg tacaaaaac 
      tccgacaaa tgcctcaat ttacgaata aaatggtcg 
      tggagtttg tacatcctg caactaatt gcagtcact 
      gtgataaca agttgggcc atttggggc ttcatcaga 
      gacatgcaa atcgccgaa ttcctaaat ctgggattc 
      acaagcgcg attgattta tacattgcg attttcgac 
      gtcaacgaa atcattatc accggattt attttcgtg 
      gcaatcatt ccatcaacc ttcaaacac ttgccagaa 
      atcatcgac aatttcaac aaaatcgac gacacgaat 
      ccgcaactc ccaacgaac aaaattcac cgcaaaata 
      ttgtttctt ttaagtttt gtgctcgtt tatatgacg 
      gtggcctac acttgcgat ttaattatg tggagccag 
      gatttattg gtcggattc attcacgat cttccctat 
      tatattttg tacagtgtt gtggtaatt cacgaggtg 
      cagtattgg tttttggtg actttaatt cgactcaga 
      atgagcgca atgaatgga cttgttgcg gaaactttg 
      aacagaaac acagatata agctcacaa gaagtgtgt 
      ttgttggta gaaacgcac tcaaacatc ctagaggcc 
      gttagcaca gttaatagg tgctttagt ggccccact 
      gcgataatt cttttgagc tgttacttg cacctggtg 
      gtgtgccct tacattctt tacgtggtg gcaagtagc 
      gataatggc ctattttgc aacacggtc tattttttg 
      tggatttcg gtccacttt tcgcgccta ttggtaata 
      atagaagtt tgcaacaat tgtgagaat gagtgtcac 
      aaaactcgc aatttaatt tttaaattg ttggctaac 
      actagtaag aaaggggac gttcgtaga gaattgaaa 
      ctgtttgca tataaaaac gtggggaaa aaagtgaga 
      ttttcatcg tatgctgtt cctaaaatt aatcggaaa 
      ctactcatt tcgattgcc acctcaatt agttcctat 
      tggatgatt ttgtaccaa tttggggct agtacaact 
      ggattgtaa
    mRNA sequence
    • >TC030128 RA
      tcatccaaa aacaatcat gaccagtag taaaccatt 
      gttaataaa ccccaatct gtttaaaac gttgcgacc 
      tctaatcaa actatcgcg tattttcgg cctgtgccc 
      cataacgta caaaaactc cgacaaatg cctcaattt 
      acgaataaa atggtcgtg gagtttgta catcctgca 
      actaattgc agtcactgt gataacaag ttgggccat 
      ttggggctt catcagaga catgcaaat cgccgaatt 
      cctaaatct gggattcac aagcgcgat tgatttata 
      cattgcgat tttcgacgt caacgaaat cattatcac 
      cggatttat tttcgtggc aatcattcc atcaacctt 
      caaacactt gccagaaat catcgacaa tttcaacaa 
      aatcgacga cacgaatcc gcaactccc aacgaacaa 
      aattcaccg caaaatatt gtttctttt aagttttgt 
      gctcgttta tatgacggt ggcctacac ttgcgattt 
      aattatgtg gagccagga tttattggt cggattcat 
      tcacgatct tccctatta tattttgta cagtgttgt 
      ggtaattca cgaggtgca gtattggtt tttggtgac 
      tttaattcg actcagaat gagcgcaat gaatggact 
      tgttgcgga aactttgaa cagaaacac agatataag 
      ctcacaaga agtgtgttt gttggtaga aacgcactc 
      aaacatcct agaggccgt tagcacagt taataggtg 
      ctttagtgg ccccactgc gataattct tttgagctg 
      ttacttgca cctggtggt gtgccctta cattcttta 
      cgtggtggc aagtagcga taatggcct attttgcaa 
      cacggtcta ttttttgtg gatttcggt ccacttttc 
      gcgcctatt ggtaataat agaagtttg caacaattg 
      tgagaatga gtgtcacaa aactcgcaa tttaatttt 
      taaattgtt ggctaacac tagtaagaa aggggacgt 
      tcgtagaga attgaaact gtttgcata taaaaacgt 
      ggggaaaaa agtgagatt ttcatcgta tgctgttcc 
      taaaattaa tcggaaact actcatttc gattgccac 
      ctcaattag ttcctattg gatgatttt gtaccaatt 
      tggggctag tacaactgg attgtaaat tattaaaaa 
      tactgatat ctaagtgtt aggataaga aaggaagta 
      caaataaaa cattcccgt taat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY