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Gene information
General information
TC015409 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 511641..515027
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015409
    iBeetle screen iB_07435

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015409 RA
      MTPHALISS DFHDVIKPL INLTKIFGF LPVCSSQKG 
      QKTCLNLSI NYCICGYIF SAILFSLSI LGLTEDLNS 
      APIRMKNPI VKYIILIDL GEVAVTVLA GILTTPFKL 
      PYFWKMLTG FGKIEQVLP PHHAPEEKK RSFWAVTIF 
      LVLAASVIF FDVVVWGLS ASDLGVFFK RFLPVYVSY 
      LLIFVQQIP FCFFVRVIR LKIEDLNGA FRAGLERVQ 
      NGENQWISV MKVNKQPKV IQGKSALSY ERLNEFMRL 
      VEEIEDVIE AINGFYGVH TLLILFSCL LHLVVTPYY 
      LLIEVCNAG YLPFILLQV AWIVLHLIR LMVIVETCQ 
      RCIDEYQVT TNLVLKLLT SPLLPDIKE QVEILAVQV 
      SNKKIKFSS FGMLKISRS LLTSFGGAL TTFLVILFQ 
      YGGIHGNVY
    CDS sequence
    • >TC015409 RA
      atgacacct cacgcttta atttcctcc gatttccac 
      gacgttatc aaaccctta atcaattta acaaaaata 
      ttcggtttc ctcccagtc tgctcgagc caaaagggc 
      caaaaaacc tgcctcaac ctctcgatt aactactgc 
      atttgtggc tacattttt agcgcaatt ctgttctcc 
      ttatccatc cttggatta accgaagac ttgaactcc 
      gccccaatc cgaatgaaa aaccccatc gtgaaatac 
      ataatcctc atcgacctt ggcgaggtc gcggtcaca 
      gttttagcc ggaattcta acaacccct ttcaaactc 
      ccctacttt tggaaaatg ttgaccgga ttcggaaaa 
      atcgagcaa gtgctgccg ccccaccac gccccagag 
      gagaaaaag cgaagtttt tgggctgtc acgattttt 
      ctggttttg gccgcaagt gtgattttt ttcgatgtg 
      gtggtgtgg ggcttgagt gcgtccgat ttgggcgtt 
      tttttcaaa cgctttttg cccgtgtat gtgtcgtat 
      ttgctcatt tttgtgcaa caaattccg ttttgtttt 
      ttcgtgcgt gttattcgc ctcaaaatt gaggatttg 
      aatggggcg ttcagggcc ggactggag cgggtacaa 
      aatggggaa aatcagtgg atttcggtc atgaaggtt 
      aacaagcaa cccaaggtt atacagggc aaaagtgcg 
      ttgagttac gaacgcttg aacgagttt atgaggctg 
      gttgaggag attgaggac gtgattgag gcaattaat 
      gggttttat ggcgttcac actttgttg attttattc 
      agctgtctt ttgcattta gtggtcact ccgtattac 
      ctgttaatt gaggtgtgt aacgctggg tatctgcct 
      tttattctg cttcaagtt gcttggatt gtgctgcat 
      ttgattcgt ttgatggta attgtggaa acttgccaa 
      cggtgtata gacgaatat caagtcacc acaaatctg 
      gttttgaaa ttattgaca agtcctcta cttccggac 
      attaaggaa caagtcgaa attttggct gtgcaagtt 
      tcgaacaaa aaaattaag ttttcatcg tttggtatg 
      ttgaagatt agtcggtct ctgttaact tcttttgga 
      ggtgccctg acgacgttt ttagtcatt ttgtttcag 
      tatggtggt attcatggg aatgtttac taa
    mRNA sequence
    • >TC015409 RA
      atttatcaa aaaataatt acatcaagt gtccagtcg 
      acctccagc tatgacacc tcacgcttt aatttcctc 
      cgatttcca cgacgttat caaaccctt aatcaattt 
      aacaaaaat attcggttt cctcccagt ctgctcgag 
      ccaaaaggg ccaaaaaac ctgcctcaa cctctcgat 
      taactactg catttgtgg ctacatttt tagcgcaat 
      tctgttctc cttatccat ccttggatt aaccgaaga 
      cttgaactc cgccccaat ccgaatgaa aaaccccat 
      cgtgaaata cataatcct catcgacct tggcgaggt 
      cgcggtcac agttttagc cggaattct aacaacccc 
      tttcaaact cccctactt ttggaaaat gttgaccgg 
      attcggaaa aatcgagca agtgctgcc gccccacca 
      cgccccaga ggagaaaaa gcgaagttt ttgggctgt 
      cacgatttt tctggtttt ggccgcaag tgtgatttt 
      tttcgatgt ggtggtgtg gggcttgag tgcgtccga 
      tttgggcgt ttttttcaa acgcttttt gcccgtgta 
      tgtgtcgta tttgctcat ttttgtgca acaaattcc 
      gttttgttt tttcgtgcg tgttattcg cctcaaaat 
      tgaggattt gaatggggc gttcagggc cggactgga 
      gcgggtaca aaatgggga aaatcagtg gatttcggt 
      catgaaggt taacaagca acccaaggt tatacaggg 
      caaaagtgc gttgagtta cgaacgctt gaacgagtt 
      tatgaggct ggttgagga gattgagga cgtgattga 
      ggcaattaa tgggtttta tggcgttca cactttgtt 
      gattttatt cagctgtct tttgcattt agtggtcac 
      tccgtatta cctgttaat tgaggtgtg taacgctgg 
      gtatctgcc ttttattct gcttcaagt tgcttggat 
      tgtgctgca tttgattcg tttgatggt aattgtgga 
      aacttgcca acggtgtat agacgaata tcaagtcac 
      cacaaatct ggttttgaa attattgac aagtcctct 
      acttccgga cattaagga acaagtcga aattttggc 
      tgtgcaagt ttcgaacaa aaaaattaa gttttcatc 
      gtttggtat gttgaagat tagtcggtc tctgttaac 
      ttcttttgg aggtgccct gacgacgtt tttagtcat 
      tttgtttca gtatggtgg tattcatgg gaatgttta 
      ctaaatagt gctaccaaa caccaaata ataaataat 
      ttacatgat g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY