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Gene information
General information
TC014646 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12805787..12807891
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014646
    iBeetle screen iB_07431

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 prothorax musculature specific subset oblique thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: or missing
    prothorax musculature specific subset oblique not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014646 RA
      MRIPVTVNP NTLKTYNCY NERRIEKNT LRSDNLSSW 
      DQAIPKTLQ ELCLESIAK NWQEKPILD EIIVTIDRC 
      FLLNILPTD LPLALTVPK IHDDIYWKR CYLQRWPKV 
      IPENVSLDV QINEIVKVS NSRKSSEES ERSRKSSAE 
      AGFKRTWKE CFLEIHICE YLEKLKPEK YDAEKIKEL 
      ADLCTDYVK ILDIRELQC SENSPNRIP LNAITSGLG 
      NLTELSICF KQTYVGEKF TWDTVRTSL QDIKLLAKG 
      LEKCKLTVF RLRNSDIDD EKAVVILKS LLKHELETL 
      DFSHCRIRD YGAVAISKF CLENSVTNL ILTNNRIGA 
      KGIEAISYA ICSPTCNLK SLDLKLNPI SQRGVEILM 
      KPLTLSARK LKILHLNAC GVGPISALG EMIEHNSHL 
      EHLDLSNNP FGEEMGKII MRGLRYNTT LKKLDVRMC 
      KLNSADEFE ILQRINKNR IAMKRPRTA RKPIIIEPQ 
      YKFEISDEL LSEMENVFQ SNKSDETTN ELIE
    CDS sequence
    • >TC014646 RA
      atgcgtatt ccggtcact gttaatcca aacactttg 
      aaaacctac aattgttac aatgaacgc agaattgag 
      aaaaatact ctacggagc gataatctg tcaagctgg 
      gatcaagcc attcccaag accttgcag gaactttgc 
      ttggaatcc atcgcaaaa aactggcaa gaaaaaccg 
      attttggac gagatcatt gtaacaatc gatcgctgc 
      tttctgtta aacatttta ccgaccgat ctccctttg 
      gctctaaca gtgccaaaa atccatgac gatatttac 
      tggaagcga tgttatttg cagcgatgg ccaaaagta 
      attcccgaa aacgtttca ctagacgtc cagataaac 
      gaaatcgtg aaagtgagc aacagcagg aaaagcagt 
      gaagagagt gaaagatcg cgaaaatct agcgctgaa 
      gcaggtttt aagaggact tggaaagag tgtttcctt 
      gaaatacat atttgcgaa tatttggag aaactgaaa 
      cctgaaaaa tacgacgct gaaaaaatc aaagaactg 
      gccgacttg tgcactgac tatgttaaa attttggat 
      atacgggaa ttgcagtgt tcggagaat tccccaaat 
      cgaattcct ctaaacgcg attacttcg gggttgggg 
      aatttaact gaactctcg atttgcttc aaacagacc 
      tacgttgga gaaaaattc acttgggac acagtcagg 
      acctcgcta caggatata aaattgttg gcaaaagga 
      ctagaaaag tgtaaatta acagttttc agactaaga 
      aattctgat attgatgac gaaaaagcc gtggtcatt 
      ttaaagtct cttctgaag catgagttg gaaacgctg 
      gatttttcc cactgtcga atccgggac tacggtgcg 
      gtggctatc agtaaattt tgcttagag aattccgtc 
      actaattta attttgacg aacaatcgt atcggagcc 
      aaaggaatt gaggccatt tcttacgca atttgtagc 
      cccacttgc aatttaaaa tctctcgat ttaaaactg 
      aatccaatt agtcaaaga ggcgtcgaa attttaatg 
      aaaccttta acactaagt gcacggaag ctcaaaatt 
      ttgcatttg aacgcttgc ggagtgggg cctatttct 
      gctttgggc gagatgatt gaacacaat tcgcatttg 
      gagcacttg gatttgagt aataatcct ttcggagag 
      gaaatgggt aagataatt atgaggggt ttgagatac 
      aatacgact ttaaaaaaa ttagatgtg agaatgtgc 
      aagttgaat tcagcggac gaattcgag attcttcaa 
      agaattaat aaaaacagg attgcgatg aaacgaccg 
      agaaccgcc agaaaacct attattatc gaaccgcaa 
      tataaattt gaaatatcg gatgaattg ttatcggag 
      atggaaaat gtatttcag agtaataaa agtgacgag 
      acaacaaat gagcttatt gaataa
    mRNA sequence
    • >TC014646 RA
      tggaaatgg ttggcgtca ttgaagaaa ttgtgagaa 
      acggattgt gtttcccgt aacgaaagg gaacgtttg 
      atcgattga ccaacaatg cgtattccg gtcactgtt 
      aatccaaac actttgaaa acctacaat tgttacaat 
      gaacgcaga attgagaaa aatactcta cggagcgat 
      aatctgtca agctgggat caagccatt cccaagacc 
      ttgcaggaa ctttgcttg gaatccatc gcaaaaaac 
      tggcaagaa aaaccgatt ttggacgag atcattgta 
      acaatcgat cgctgcttt ctgttaaac attttaccg 
      accgatctc cctttggct ctaacagtg ccaaaaatc 
      catgacgat atttactgg aagcgatgt tatttgcag 
      cgatggcca aaagtaatt cccgaaaac gtttcacta 
      gacgtccag ataaacgaa atcgtgaaa gtgagcaac 
      agcaggaaa agcagtgaa gagagtgaa agatcgcga 
      aaatctagc gctgaagca ggttttaag aggacttgg 
      aaagagtgt ttccttgaa atacatatt tgcgaatat 
      ttggagaaa ctgaaacct gaaaaatac gacgctgaa 
      aaaatcaaa gaactggcc gacttgtgc actgactat 
      gttaaaatt ttggatata cgggaattg cagtgttcg 
      gagaattcc ccaaatcga attcctcta aacgcgatt 
      acttcgggg ttggggaat ttaactgaa ctctcgatt 
      tgcttcaaa cagacctac gttggagaa aaattcact 
      tgggacaca gtcaggacc tcgctacag gatataaaa 
      ttgttggca aaaggacta gaaaagtgt aaattaaca 
      gttttcaga ctaagaaat tctgatatt gatgacgaa 
      aaagccgtg gtcatttta aagtctctt ctgaagcat 
      gagttggaa acgctggat ttttcccac tgtcgaatc 
      cgggactac ggtgcggtg gctatcagt aaattttgc 
      ttagagaat tccgtcact aatttaatt ttgacgaac 
      aatcgtatc ggagccaaa ggaattgag gccatttct 
      tacgcaatt tgtagcccc acttgcaat ttaaaatct 
      ctcgattta aaactgaat ccaattagt caaagaggc 
      gtcgaaatt ttaatgaaa cctttaaca ctaagtgca 
      cggaagctc aaaattttg catttgaac gcttgcgga 
      gtggggcct atttctgct ttgggcgag atgattgaa 
      cacaattcg catttggag cacttggat ttgagtaat 
      aatcctttc ggagaggaa atgggtaag ataattatg 
      aggggtttg agatacaat acgacttta aaaaaatta 
      gatgtgaga atgtgcaag ttgaattca gcggacgaa 
      ttcgagatt cttcaaaga attaataaa aacaggatt 
      gcgatgaaa cgaccgaga accgccaga aaacctatt 
      attatcgaa ccgcaatat aaatttgaa atatcggat 
      gaattgtta tcggagatg gaaaatgta tttcagagt 
      aataaaagt gacgagaca acaaatgag cttattgaa 
      taataaatt gaagtcgct ttttgtcaa ttttataaa 
      taaagtatt tcacgtctg tggattttc atttataat 
      aaaaagttc aatggcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY