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Gene information
General information
TC014076 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5389550..5393784
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014076
    iBeetle screen iB_07430

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature fluorescence broader - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Larvae look wildtypish within their eggshells. As soon as they hatch they seem to be dieing
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: flour poorly removed,
    flour could not be removed from the well for reasons unknown. In other (as well as adjacent) wells flour was sufficiently removed

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen posterior shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks - cuticles: flour poorly removed,
    flour could not be removed from the well for reasons unknown. In other (as well as adjacent) wells flour was sufficiently removed

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014076 RA
      MAQHILSAV ESEITSGGK SKGDPTERD LIVTLDDRD 
      LWLRFECLT NEMIVTKNG RRMFPVVKV TAAGLDPKA 
      MYTVLLEFV QIDPHRWKY VNGEWVPGG KAEVPPSNP 
      IYIHPESPN FGAHWMKES ISFAKVKLT NKSNGNGQI 
      MLNSLHKYK PTVHLVRVG TEPRRVISF PFPETQFIA 
      VTAYQNEEV TALKIKYNP FAKAFLDAK ERPDSIYQR 
      EYSPTFTQQ QTQYSQYGS WFLPHQPIY PAVTATVQL 
      STCTRTTLK NNRASPYPM QRPKSASLS PPPHVYNPP 
      DHIQQATPY SAPGPTYST WSTVQTPTT VMSPTINCW 
      SLTSSPPPP YQISAQPTP SQSPNQIYQ PVTNLTNLT 
      YPSYYNQDV SCTHYQNPE YISVVNPES PYPQIGSER 
      ATPVGYHQN EVGGVIDDG GSGQNQPES PESSSSTPR 
      HEWVAQNHL
    CDS sequence
    • >TC014076 RA
      atggcacag cacatcctc tccgccgtc gaatccgaa 
      atcacgtcc ggtggaaaa agcaagggg gatccaact 
      gaacgcgat ttgatcgtc actcttgat gatcgagac 
      ctttggctg cgatttgag tgtttgacg aacgaaatg 
      atcgtcacg aagaacggc cggcgcatg tttcccgtg 
      gtgaaggtc accgccgca ggcctcgac cccaaggcc 
      atgtacacc gtcctcctg gagttcgtc cagatcgac 
      cctcaccgc tggaaatac gtaaacggg gaatgggtt 
      cctggaggc aaagcagaa gttccccct tcaaacccc 
      atctacatc catcccgag tctccaaac ttcggtgcc 
      cactggatg aaagaatcc atctcgttc gcaaaagtc 
      aaacttacg aacaaatcg aacggaaac ggtcaaatt 
      atgctcaac tccctccac aaatacaaa cccactgtt 
      cacctggtc cgagtaggc actgaaccc cgaagagtc 
      atatcgttt ccgtttccg gaaacacaa ttcattgcc 
      gttaccgct taccagaac gaagaagtg accgcgtta 
      aaaattaag tacaatccg ttcgccaaa gccttcctt 
      gatgcgaag gaacgcccg gatagtata taccagcgg 
      gagtactcg cccaccttc acccaacaa caaacccag 
      tactcacaa tatgggtca tggttcttg ccccatcaa 
      cccatctac ccggccgtg accgcaacc gtacaacta 
      tcaacatgc acaaggacc acattgaaa aacaatcga 
      gcatcgcct tatcccatg caacgcccc aagtcggcg 
      tccctctca cccccacca cacgtgtac aaccctccg 
      gatcacatc cagcaagcc acgccttac agtgcacca 
      ggccccacc tactccacc tggtccacg gtccagacc 
      cccaccacc gtaatgagc cccacgatc aactgctgg 
      tccctcact tcaagcccc ccaccaccg tatcaaatc 
      tccgcccaa cccactcca agccaatcc cccaaccaa 
      atctaccaa cccgtcaca aacctaacc aacctaaca 
      tatcccagt tattacaac caggacgtt tcatgcaca 
      cactaccaa aatcccgag tacatctcg gtggtcaac 
      ccggaaagc ccctacccc caaattggg tcagagcga 
      gccacgcct gttggctac caccagaac gaagtgggc 
      ggagttatc gacgacgga gggagtggc caaaaccag 
      ccggagtcg cccgaatcc agctcgagc acaccgcga 
      cacgaatgg gtcgctcaa aatcattta taa
    mRNA sequence
    • >TC014076 RA
      agaggtgac caatagaaa tctgtgggt ggggtctat 
      gggtttcca tcgatggca gcagcagcc cgaaagttt 
      tagtgatgt tggcgataa tatgttaag atctctaaa 
      atgtaagaa gtacttatc gatttggtg tgaaataat 
      gacaaataa tatataata aacgtttaa tttacaaaa 
      tcccgagct gtgtgttta tcaaataag catctagtg 
      tatgattga cattttaaa taactaaat gtatcaatt 
      aattatgtt aattttttt aataaatcg gtgtgaaat 
      ttaattgtg cgacgccaa gtttaacgc catacatga 
      ggaacgcag tttagactt cgcaatggc acagcacat 
      cctctccgc cgtcgaatc cgaaatcac gtccggtgg 
      aaaaagcaa gggggatcc aactgaacg cgatttgat 
      cgtcactct tgatgatcg agacctttg gctgcgatt 
      tgagtgttt gacgaacga aatgatcgt cacgaagaa 
      cggccggcg catgtttcc cgtggtgaa ggtcaccgc 
      cgcaggcct cgaccccaa ggccatgta caccgtcct 
      cctggagtt cgtccagat cgaccctca ccgctggaa 
      atacgtaaa cggggaatg ggttcctgg aggcaaagc 
      agaagttcc cccttcaaa ccccatcta catccatcc 
      cgagtctcc aaacttcgg tgcccactg gatgaaaga 
      atccatctc gttcgcaaa agtcaaact tacgaacaa 
      atcgaacgg aaacggtca aattatgct caactccct 
      ccacaaata caaacccac tgttcacct ggtccgagt 
      aggcactga accccgaag agtcatatc gtttccgtt 
      tccggaaac acaattcat tgccgttac cgcttacca 
      gaacgaaga agtgaccgc gttaaaaat taagtacaa 
      tccgttcgc caaagcctt ccttgatgc gaaggaacg 
      cccggatag tatatacca gcgggagta ctcgcccac 
      cttcaccca acaacaaac ccagtactc acaatatgg 
      gtcatggtt cttgcccca tcaacccat ctacccggc 
      cgtgaccgc aaccgtaca actatcaac atgcacaag 
      gaccacatt gaaaaacaa tcgagcatc gccttatcc 
      catgcaacg ccccaagtc ggcgtccct ctcaccccc 
      accacacgt gtacaaccc tccggatca catccagca 
      agccacgcc ttacagtgc accaggccc cacctactc 
      cacctggtc cacggtcca gacccccac caccgtaat 
      gagccccac gatcaactg ctggtccct cacttcaag 
      ccccccacc accgtatca aatctccgc ccaacccac 
      tccaagcca atcccccaa ccaaatcta ccaacccgt 
      cacaaacct aaccaacct aacatatcc cagttatta 
      caaccagga cgtttcatg cacacacta ccaaaatcc 
      cgagtacat ctcggtggt caacccgga aagccccta 
      cccccaaat tgggtcaga gcgagccac gcctgttgg 
      ctaccacca gaacgaagt gggcggagt tatcgacga 
      cggagggag tggccaaaa ccagccgga gtcgcccga 
      atccagctc gagcacacc gcgacacga atgggtcgc 
      tcaaaatca tttataaac ttgtaaaat tgtaaataa 
      aggagtgaa aaccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY