Status
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Gene information
General information
TC009341 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 584375..590132
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009341
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07411

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009341 RA
      MNTSQINTQ GINDAGSLC QDPATRGFD LQAGQTWIY 
      PTNLPIREY QYTITEKAL HWNTLVSLP TGLGKTFIA 
      AVVMYNFYR WYPHDKVIF MAPTRPLVK QQMDACYNI 
      MAIPQEVTA ELTGTKIQQ SRTEIWQNK RVFFITPQV 
      LQNDLNIIV ELGPKIKCL VFDEAHRAK GNHAYCEVV 
      RKLLPENKL FRVLALSAT PGNSSKDVL DVMRNLLIA 
      HLEFRSEES PDVKPYVFE RALETVVVP LGEKLQQIR 
      DKYIQVLEK YTRTLIQYK VIYGNCANL TKGKIFMVM 
      KEFQAKNSA NRTANYAEI MKCLNICVT LYHAYETLV 
      RCGLRAFLN FYKEHINNP LLQGNVSII QIMDDLQAY 
      LGPDPEVQP LPDGTFAEI PASIKFGHP KFYQLRDIL 
      VAHFTNSDA STRVIVFFE YRESATEAY ALLTRSFPL 
      IRSRVFVGQ RAGVTQKEQ INTVKSFRE GTCNTLLST 
      CIGEEGLDV GEVDLIVCF DIANKSPIR MIQRMGRTG 
      RKKEGRVVV LVTEGREQQ TLKDCLIYK NNLGSFATN 
      SQLLQEGKY TDNPKMIPS YVQPKCQKM CITVKKPVV 
      TKTANLKDM LRNISDDLE IVEIKERIS DKIDNFWNK 
      TREEFFQIE AKPKIDFAK HLEKQRVFQ ANSKSKQTE 
      IFVTLLQFA DSKRFNLPV TQKSIFSDF NQHKYLKQG 
      DIRSMFAKP NVNALSQEP VSQKFSFGG EKDVTQSFP 
      PELFEELST FLSIQDLYR CKTCEFLFE CPTIKYNKT 
      RDTLDLSSF TVPDLSVVE SITIKDLEV FADSLSPKS 
      REKHQELME VDDSFDDID LSELEEMCL EKNVPYETP 
      RNFDSFMDS FRIRDDQQI EKVPEKSYI FEPPETFNK 
      VLNLLEPKE DYNDVLAFF KLSSVAEIF EETGHTLEK 
      CEETPADLD ETVIYTPEK CANTSPDIF EGSPDLFPS 
      DEKQDVSFE APLSPILCS YTRVQELSA KKKLFEEKK 
      PNLANLNQR RKSFFETTN AQVSSPKKN FDIISDICD 
      LSQLLNNRE ENKEKVEST KVDLDISDL CDLSAFGLS 
      RKNVESTLP KEEEKPKLD LSIDDLCDM SQFSVKKAE 
      TQTTSHKNI EEQLSSLDD FCDFSCFLP DSNKETHRK 
      SISDKGIIR PQTQNLNVS TIDLTESPV KEPSNTDNN 
      KSQLSVTQM LSLIAKSDS QKENTTPKT KKSQRLTQS 
      RQKCETPKS TQKKSSRVK KSPKFNISF DGDSEDEFE 
      EKSPIFAKP RTISLKNKT EKPSKTKER RKLVCEFLD 
      EEAVVSGDE QTISDDEDS GEDCFEASF VADETEDLL 
      NDTIMHARY LQSVKSPVR GKFKIPDKP TFNVSNVFS 
      QQVSNEDDT YMNDSFCVS SQEVETCSN DLSELELLE 
      MQLKEEKQN KKRKKSDSK SAAKKRRIV VLDDSD
    CDS sequence
    • >TC009341 RA
      atgaacact tcgcaaata aacacacaa ggaatcaac 
      gatgctgga agcctgtgc caggacccc gccaccaga 
      ggcttcgac ttacaagct ggccagaca tggatttac 
      cccacaaat ctcccaatc cgggagtac caatacact 
      ataaccgaa aaagccctc cactggaat accctcgta 
      agtcttccg acaggtctc ggcaaaacc ttcatcgca 
      gccgtggtc atgtacaac ttctaccga tggtacccc 
      catgacaag gtcattttc atggccccc acacgcccc 
      ctagtcaag caacaaatg gacgcgtgc tacaacatc 
      atggccatc cctcaggag gtcacagcc gaactcact 
      ggcaccaaa atccagcaa tcacgtacc gaaatctgg 
      caaaataaa cgagttttt tttatcaca ccgcaggtt 
      ttgcaaaac gatcttaac ataattgtc gagttgggg 
      ccgaaaatt aagtgtttg gtgtttgat gaggcgcat 
      agagcaaag ggtaatcac gcctattgt gaagttgtg 
      cgtaagtta ttaccggaa aataagtta tttcgggtg 
      ttggcgttg agcgctaca cccgggaat agttcgaag 
      gatgtgctg gatgttatg aggaatttg ttgattgcg 
      catttggaa ttcaggagt gaggaaagt cccgatgtg 
      aagccgtat gtgtttgag agggcgctt gagactgtt 
      gtggtgccg ttgggtgag aagttgcaa caaatcagg 
      gataagtac attcaggtt ttagaaaaa tacaccaga 
      accctaatc cagtataaa gtgatttat ggaaattgt 
      gctaatcta accaaaggt aaaatattc atggtgatg 
      aaggaattc caggcgaaa aattcagcc aatagaact 
      gcaaactat gcagaaatt atgaaatgt ctaaacatt 
      tgtgtcaca ctttatcat gcctatgaa acccttgtt 
      cgttgtggt ttgagggcg tttctgaac ttttacaaa 
      gaacatatt aataatcct ttactacag ggaaatgtg 
      agtataatt cagataatg gacgacctt caagcatac 
      ttaggtcca gaccctgaa gtccaacca ctgcccgat 
      ggtactttt gctgagatt ccagcttca ataaaattt 
      gggcacccg aagttttac caacttcga gacatcctt 
      gttgcacat ttcacaaat tcggacgct tccactcgt 
      gttattgtg ttttttgag tatcgggag agcgccacc 
      gaagcttac gctttactc actaggagt tttcctctg 
      ataaggtcc cgcgttttt gttggtcaa cgtgccgga 
      gtgacacaa aaagagcaa ataaatacg gtcaaaagt 
      ttcagagaa ggcacttgc aataccctc ctgtcgact 
      tgtattggc gaagaaggc cttgatgtg ggcgaagtc 
      gacttgatt gtttgcttc gatatagcg aataaatct 
      ccaattcga atgatacaa agaatgggg cggaccggg 
      cgtaaaaaa gaagggcgg gttgtggtt ctggttacg 
      gaagggagg gaacaacaa acgttgaaa gattgtttg 
      atttataaa aataacttg gggagtttt gcaacgaat 
      tcgcaattg ttgcaagag gggaaatac acagataat 
      cccaagatg ataccttcg tacgtgcaa ccaaagtgc 
      caaaaaatg tgcatcact gttaaaaaa cccgtcgta 
      acaaaaacc gccaatttg aaagatatg ttacgaaac 
      atttccgat gatttagaa attgttgaa attaaggaa 
      cgaatctct gataagatt gataatttc tggaacaaa 
      actagagaa gaattcttc caaatcgag gcaaaaccc 
      aaaattgat tttgccaaa catttggag aaacaaagg 
      gtgttccaa gctaactca aaatccaaa caaacggaa 
      attttcgtt acgttacta cagtttgcc gattctaag 
      cgtttcaac ttaccagtg acacaaaaa tcaatattt 
      agcgatttc aatcaacat aaatatttg aaacaaggt 
      gatatcagg agtatgttt gccaagccc aatgtcaat 
      gctttgagc caagaacct gtaagccag aaattttct 
      tttgggggc gaaaaagac gtgactcag tcgtttccg 
      cctgaatta ttcgaagaa ttgtcaacg tttttatca 
      atacaagac ttgtaccgt tgcaaaacg tgcgaattt 
      ttattcgag tgtcctaca atcaaatat aacaaaact 
      cgtgatact ttggatttg agttctttc acagttcct 
      gatttatca gtagttgaa agtatcacc atcaaagat 
      ttggaggtt tttgctgat tctttgagt ccaaaatcg 
      agagaaaaa caccaagag ttgatggag gttgatgat 
      agtttcgac gatattgat ttaagtgaa ctggaagaa 
      atgtgtctg gaaaaaaat gtaccgtat gaaacccca 
      cgaaacttt gattctttt atggattcg tttcgcata 
      cgcgacgat caacaaatt gagaaagtt cccgaaaaa 
      tcgtacatt tttgaacca cctgaaacg ttcaataaa 
      gttttgaat ttattggaa ccgaaggaa gactataac 
      gacgttttg gcatttttt aaactctca tcagttgct 
      gaaattttc gaagaaact ggacacact cttgaaaag 
      tgtgaagaa acaccggca gatttggat gaaactgtt 
      atttacact cccgaaaag tgtgcaaac acctctcct 
      gatattttc gaagggtca ccagattta ttcccttct 
      gacgaaaaa caagacgtt tcattcgaa gcaccctta 
      tcgccgatt ttatgcagc tacacaagg gtgcaagag 
      ttaagtgcg aaaaagaaa ttgtttgag gaaaaaaaa 
      ccgaattta gcaaacttg aaccaacga cgaaaaagt 
      ttttttgaa accacaaat gctcaagtt tcgtcgccc 
      aaaaagaat tttgatatt attagcgat atttgtgat 
      ttatcgcag cttttgaat aatcgagaa gaaaacaag 
      gaaaaagtt gagtctacg aaagtagat ttggatatt 
      agtgatctt tgcgatttg tctgcgttt ggattgtcg 
      cgaaaaaat gttgaaagc actttgcca aaggaagag 
      gaaaagcca aaactggat ttatctatc gatgatttg 
      tgcgacatg tcgcagttt tcagtcaaa aaagcggaa 
      actcaaact acgagccat aaaaatatt gaagaacaa 
      ctctcgagt cttgatgat ttttgcgat ttttcttgt 
      ttccttcct gattcgaat aaagaaact cacaggaaa 
      tcaatttcg gataagggg ataattaga ccacaaact 
      cagaacttg aatgttagt acgattgat ttgactgag 
      agtcccgtt aaagaacct tctaacaca gataataat 
      aaatctcaa ctttcggta actcagatg ttaagttta 
      atcgcgaaa agcgatagt cagaaagaa aatacgact 
      ccaaaaacg aaaaaatcc cagcgttta acacaatca 
      agacaaaaa tgtgagact cctaaatct acacaaaag 
      aaaagttct cgagtaaag aaaagcccc aagtttaat 
      atttcgttt gatggggac agtgaggac gagttcgag 
      gaaaagagt ccaattttt gcaaaacct agaactatt 
      tcactgaag aacaaaact gagaaaccg tcaaaaacg 
      aaagagaga cgcaaatta gtgtgcgag tttctggat 
      gaagaagcg gtcgtatct ggtgatgaa caaaccatt 
      tcggatgat gaagatagt ggggaagat tgttttgaa 
      gcaagtttt gtagctgat gaaacggag gatttgctt 
      aatgataca ataatgcat gcgagatat ttacaatct 
      gtgaaaagt ccagtgcga gggaagttc aaaattccg 
      gacaaacct acgtttaat gtctcaaat gtgttctct 
      caacaagtt agcaatgaa gatgacacg tatatgaac 
      gattcgttc tgcgtgagc agccaagag gttgaaact 
      tgcagcaac gacttgtcc gagctggaa ctactggaa 
      atgcagctg aaggaggaa aaacaaaac aaaaaacga 
      aaaaaatcg gacagtaaa agcgccgcg aaaaagcga 
      agaatagtt gtgttggac gacagtgat taa
    mRNA sequence
    • >TC009341 RA
      atcgtgatg ttgacaatt caaaaaact aacctcaca 
      ctgcaatat tcaaacttg tttgttctt gttattctg 
      ttcgtgatg taattataa tgaacactt cgcaaataa 
      acacacaag gaatcaacg atgctggaa gcctgtgcc 
      aggaccccg ccaccagag gcttcgact tacaagctg 
      gccagacat ggatttacc ccacaaatc tcccaatcc 
      gggagtacc aatacacta taaccgaaa aagccctcc 
      actggaata ccctcgtaa gtcttccga caggtctcg 
      gcaaaacct tcatcgcag ccgtggtca tgtacaact 
      tctaccgat ggtaccccc atgacaagg tcattttca 
      tggccccca cacgccccc tagtcaagc aacaaatgg 
      acgcgtgct acaacatca tggccatcc ctcaggagg 
      tcacagccg aactcactg gcaccaaaa tccagcaat 
      cacgtaccg aaatctggc aaaataaac gagtttttt 
      ttatcacac cgcaggttt tgcaaaacg atcttaaca 
      taattgtcg agttggggc cgaaaatta agtgtttgg 
      tgtttgatg aggcgcata gagcaaagg gtaatcacg 
      cctattgtg aagttgtgc gtaagttat taccggaaa 
      ataagttat ttcgggtgt tggcgttga gcgctacac 
      ccgggaata gttcgaagg atgtgctgg atgttatga 
      ggaatttgt tgattgcgc atttggaat tcaggagtg 
      aggaaagtc ccgatgtga agccgtatg tgtttgaga 
      gggcgcttg agactgttg tggtgccgt tgggtgaga 
      agttgcaac aaatcaggg ataagtaca ttcaggttt 
      tagaaaaat acaccagaa ccctaatcc agtataaag 
      tgatttatg gaaattgtg ctaatctaa ccaaaggta 
      aaatattca tggtgatga aggaattcc aggcgaaaa 
      attcagcca atagaactg caaactatg cagaaatta 
      tgaaatgtc taaacattt gtgtcacac tttatcatg 
      cctatgaaa cccttgttc gttgtggtt tgagggcgt 
      ttctgaact tttacaaag aacatatta ataatcctt 
      tactacagg gaaatgtga gtataattc agataatgg 
      acgaccttc aagcatact taggtccag accctgaag 
      tccaaccac tgcccgatg gtacttttg ctgagattc 
      cagcttcaa taaaatttg ggcacccga agttttacc 
      aacttcgag acatccttg ttgcacatt tcacaaatt 
      cggacgctt ccactcgtg ttattgtgt tttttgagt 
      atcgggaga gcgccaccg aagcttacg ctttactca 
      ctaggagtt ttcctctga taaggtccc gcgtttttg 
      ttggtcaac gtgccggag tgacacaaa aagagcaaa 
      taaatacgg tcaaaagtt tcagagaag gcacttgca 
      ataccctcc tgtcgactt gtattggcg aagaaggcc 
      ttgatgtgg gcgaagtcg acttgattg tttgcttcg 
      atatagcga ataaatctc caattcgaa tgatacaaa 
      gaatggggc ggaccgggc gtaaaaaag aagggcggg 
      ttgtggttc tggttacgg aagggaggg aacaacaaa 
      cgttgaaag attgtttga tttataaaa ataacttgg 
      ggagttttg caacgaatt cgcaattgt tgcaagagg 
      ggaaataca cagataatc ccaagatga taccttcgt 
      acgtgcaac caaagtgcc aaaaaatgt gcatcactg 
      ttaaaaaac ccgtcgtaa caaaaaccg ccaatttga 
      aagatatgt tacgaaaca tttccgatg atttagaaa 
      ttgttgaaa ttaaggaac gaatctctg ataagattg 
      ataatttct ggaacaaaa ctagagaag aattcttcc 
      aaatcgagg caaaaccca aaattgatt ttgccaaac 
      atttggaga aacaaaggg tgttccaag ctaactcaa 
      aatccaaac aaacggaaa ttttcgtta cgttactac 
      agtttgccg attctaagc gtttcaact taccagtga 
      cacaaaaat caatattta gcgatttca atcaacata 
      aatatttga aacaaggtg atatcagga gtatgtttg 
      ccaagccca atgtcaatg ctttgagcc aagaacctg 
      taagccaga aattttctt ttgggggcg aaaaagacg 
      tgactcagt cgtttccgc ctgaattat tcgaagaat 
      tgtcaacgt ttttatcaa tacaagact tgtaccgtt 
      gcaaaacgt gcgaatttt tattcgagt gtcctacaa 
      tcaaatata acaaaactc gtgatactt tggatttga 
      gttctttca cagttcctg atttatcag tagttgaaa 
      gtatcacca tcaaagatt tggaggttt ttgctgatt 
      ctttgagtc caaaatcga gagaaaaac accaagagt 
      tgatggagg ttgatgata gtttcgacg atattgatt 
      taagtgaac tggaagaaa tgtgtctgg aaaaaaatg 
      taccgtatg aaaccccac gaaactttg attctttta 
      tggattcgt ttcgcatac gcgacgatc aacaaattg 
      agaaagttc ccgaaaaat cgtacattt ttgaaccac 
      ctgaaacgt tcaataaag ttttgaatt tattggaac 
      cgaaggaag actataacg acgttttgg catttttta 
      aactctcat cagttgctg aaattttcg aagaaactg 
      gacacactc ttgaaaagt gtgaagaaa caccggcag 
      atttggatg aaactgtta tttacactc ccgaaaagt 
      gtgcaaaca cctctcctg atattttcg aagggtcac 
      cagatttat tcccttctg acgaaaaac aagacgttt 
      cattcgaag cacccttat cgccgattt tatgcagct 
      acacaaggg tgcaagagt taagtgcga aaaagaaat 
      tgtttgagg aaaaaaaac cgaatttag caaacttga 
      accaacgac gaaaaagtt tttttgaaa ccacaaatg 
      ctcaagttt cgtcgccca aaaagaatt ttgatatta 
      ttagcgata tttgtgatt tatcgcagc ttttgaata 
      atcgagaag aaaacaagg aaaaagttg agtctacga 
      aagtagatt tggatatta gtgatcttt gcgatttgt 
      ctgcgtttg gattgtcgc gaaaaaatg ttgaaagca 
      ctttgccaa aggaagagg aaaagccaa aactggatt 
      tatctatcg atgatttgt gcgacatgt cgcagtttt 
      cagtcaaaa aagcggaaa ctcaaacta cgagccata 
      aaaatattg aagaacaac tctcgagtc ttgatgatt 
      tttgcgatt tttcttgtt tccttcctg attcgaata 
      aagaaactc acaggaaat caatttcgg ataagggga 
      taattagac cacaaactc agaacttga atgttagta 
      cgattgatt tgactgaga gtcccgtta aagaacctt 
      ctaacacag ataataata aatctcaac tttcggtaa 
      ctcagatgt taagtttaa tcgcgaaaa gcgatagtc 
      agaaagaaa atacgactc caaaaacga aaaaatccc 
      agcgtttaa cacaatcaa gacaaaaat gtgagactc 
      ctaaatcta cacaaaaga aaagttctc gagtaaaga 
      aaagcccca agtttaata tttcgtttg atggggaca 
      gtgaggacg agttcgagg aaaagagtc caatttttg 
      caaaaccta gaactattt cactgaaga acaaaactg 
      agaaaccgt caaaaacga aagagagac gcaaattag 
      tgtgcgagt ttctggatg aagaagcgg tcgtatctg 
      gtgatgaac aaaccattt cggatgatg aagatagtg 
      gggaagatt gttttgaag caagttttg tagctgatg 
      aaacggagg atttgctta atgatacaa taatgcatg 
      cgagatatt tacaatctg tgaaaagtc cagtgcgag 
      ggaagttca aaattccgg acaaaccta cgtttaatg 
      tctcaaatg tgttctctc aacaagtta gcaatgaag 
      atgacacgt atatgaacg attcgttct gcgtgagca 
      gccaagagg ttgaaactt gcagcaacg acttgtccg 
      agctggaac tactggaaa tgcagctga aggaggaaa 
      aacaaaaca aaaaacgaa aaaaatcgg acagtaaaa 
      gcgccgcga aaaagcgaa gaatagttg tgttggacg 
      acagtgatt aaataattt tttattaat aatcgttaa 
      atattagtt taatgtcct caaaaaccg catcctctg 
      acttgtttc catcgacga ctctcaaca aattcaatt 
      ctctgtaat aatcaaccg ttggttgag tctccttaa 
      ggtgtgtgg ccacccgtt tttcaataa cttcaatcg 
      tgtcgtcaa aacgcccac ttccagccg cagcccctc 
      tattgaggc acctctcaa tacaaacct ccacaggac 
      aatcaataa ataaaacga acataactt caactttat 
      ccccaattg tcctgctcc aaccttcgc gattttcca 
      aattccgcg ggaatccgt caatgagaa aacggtctt 
      gttgcgact cctccatgt gccgttcca gcaacttgc 
      aagtgattt ctgccggaa ctatcttcc cgtctttta 
      tacaagaat caatcagtt cgccgtgaa ccgaattgc 
      ggacttgtc gctcacgcc tcagcaact caccggccg 
      aaatgtgta caaaaccgt aataactgg ctattttac 
      gacattgtg tccctttac caacccccg gcggcccca 
      aaacaaaca caactttct tgtgcccca ttttcaagc 
      cggtcggta acacccgtc cctaccaaa ccggccaag 
      ccaaaacac ggaaaaaat cgaaaaatc gctttattg 
      gcccgcgtt tgcaccaaa gatt
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