| General information | |||
| TC009341 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 584375..590132 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009341 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07411 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC009341 RA
MNTSQINTQ GINDAGSLC QDPATRGFD LQAGQTWIY PTNLPIREY QYTITEKAL HWNTLVSLP TGLGKTFIA AVVMYNFYR WYPHDKVIF MAPTRPLVK QQMDACYNI MAIPQEVTA ELTGTKIQQ SRTEIWQNK RVFFITPQV LQNDLNIIV ELGPKIKCL VFDEAHRAK GNHAYCEVV RKLLPENKL FRVLALSAT PGNSSKDVL DVMRNLLIA HLEFRSEES PDVKPYVFE RALETVVVP LGEKLQQIR DKYIQVLEK YTRTLIQYK VIYGNCANL TKGKIFMVM KEFQAKNSA NRTANYAEI MKCLNICVT LYHAYETLV RCGLRAFLN FYKEHINNP LLQGNVSII QIMDDLQAY LGPDPEVQP LPDGTFAEI PASIKFGHP KFYQLRDIL VAHFTNSDA STRVIVFFE YRESATEAY ALLTRSFPL IRSRVFVGQ RAGVTQKEQ INTVKSFRE GTCNTLLST CIGEEGLDV GEVDLIVCF DIANKSPIR MIQRMGRTG RKKEGRVVV LVTEGREQQ TLKDCLIYK NNLGSFATN SQLLQEGKY TDNPKMIPS YVQPKCQKM CITVKKPVV TKTANLKDM LRNISDDLE IVEIKERIS DKIDNFWNK TREEFFQIE AKPKIDFAK HLEKQRVFQ ANSKSKQTE IFVTLLQFA DSKRFNLPV TQKSIFSDF NQHKYLKQG DIRSMFAKP NVNALSQEP VSQKFSFGG EKDVTQSFP PELFEELST FLSIQDLYR CKTCEFLFE CPTIKYNKT RDTLDLSSF TVPDLSVVE SITIKDLEV FADSLSPKS REKHQELME VDDSFDDID LSELEEMCL EKNVPYETP RNFDSFMDS FRIRDDQQI EKVPEKSYI FEPPETFNK VLNLLEPKE DYNDVLAFF KLSSVAEIF EETGHTLEK CEETPADLD ETVIYTPEK CANTSPDIF EGSPDLFPS DEKQDVSFE APLSPILCS YTRVQELSA KKKLFEEKK PNLANLNQR RKSFFETTN AQVSSPKKN FDIISDICD LSQLLNNRE ENKEKVEST KVDLDISDL CDLSAFGLS RKNVESTLP KEEEKPKLD LSIDDLCDM SQFSVKKAE TQTTSHKNI EEQLSSLDD FCDFSCFLP DSNKETHRK SISDKGIIR PQTQNLNVS TIDLTESPV KEPSNTDNN KSQLSVTQM LSLIAKSDS QKENTTPKT KKSQRLTQS RQKCETPKS TQKKSSRVK KSPKFNISF DGDSEDEFE EKSPIFAKP RTISLKNKT EKPSKTKER RKLVCEFLD EEAVVSGDE QTISDDEDS GEDCFEASF VADETEDLL NDTIMHARY LQSVKSPVR GKFKIPDKP TFNVSNVFS QQVSNEDDT YMNDSFCVS SQEVETCSN DLSELELLE MQLKEEKQN KKRKKSDSK SAAKKRRIV VLDDSD
- >TC009341 RA
atgaacact tcgcaaata aacacacaa ggaatcaac gatgctgga agcctgtgc caggacccc gccaccaga ggcttcgac ttacaagct ggccagaca tggatttac cccacaaat ctcccaatc cgggagtac caatacact ataaccgaa aaagccctc cactggaat accctcgta agtcttccg acaggtctc ggcaaaacc ttcatcgca gccgtggtc atgtacaac ttctaccga tggtacccc catgacaag gtcattttc atggccccc acacgcccc ctagtcaag caacaaatg gacgcgtgc tacaacatc atggccatc cctcaggag gtcacagcc gaactcact ggcaccaaa atccagcaa tcacgtacc gaaatctgg caaaataaa cgagttttt tttatcaca ccgcaggtt ttgcaaaac gatcttaac ataattgtc gagttgggg ccgaaaatt aagtgtttg gtgtttgat gaggcgcat agagcaaag ggtaatcac gcctattgt gaagttgtg cgtaagtta ttaccggaa aataagtta tttcgggtg ttggcgttg agcgctaca cccgggaat agttcgaag gatgtgctg gatgttatg aggaatttg ttgattgcg catttggaa ttcaggagt gaggaaagt cccgatgtg aagccgtat gtgtttgag agggcgctt gagactgtt gtggtgccg ttgggtgag aagttgcaa caaatcagg gataagtac attcaggtt ttagaaaaa tacaccaga accctaatc cagtataaa gtgatttat ggaaattgt gctaatcta accaaaggt aaaatattc atggtgatg aaggaattc caggcgaaa aattcagcc aatagaact gcaaactat gcagaaatt atgaaatgt ctaaacatt tgtgtcaca ctttatcat gcctatgaa acccttgtt cgttgtggt ttgagggcg tttctgaac ttttacaaa gaacatatt aataatcct ttactacag ggaaatgtg agtataatt cagataatg gacgacctt caagcatac ttaggtcca gaccctgaa gtccaacca ctgcccgat ggtactttt gctgagatt ccagcttca ataaaattt gggcacccg aagttttac caacttcga gacatcctt gttgcacat ttcacaaat tcggacgct tccactcgt gttattgtg ttttttgag tatcgggag agcgccacc gaagcttac gctttactc actaggagt tttcctctg ataaggtcc cgcgttttt gttggtcaa cgtgccgga gtgacacaa aaagagcaa ataaatacg gtcaaaagt ttcagagaa ggcacttgc aataccctc ctgtcgact tgtattggc gaagaaggc cttgatgtg ggcgaagtc gacttgatt gtttgcttc gatatagcg aataaatct ccaattcga atgatacaa agaatgggg cggaccggg cgtaaaaaa gaagggcgg gttgtggtt ctggttacg gaagggagg gaacaacaa acgttgaaa gattgtttg atttataaa aataacttg gggagtttt gcaacgaat tcgcaattg ttgcaagag gggaaatac acagataat cccaagatg ataccttcg tacgtgcaa ccaaagtgc caaaaaatg tgcatcact gttaaaaaa cccgtcgta acaaaaacc gccaatttg aaagatatg ttacgaaac atttccgat gatttagaa attgttgaa attaaggaa cgaatctct gataagatt gataatttc tggaacaaa actagagaa gaattcttc caaatcgag gcaaaaccc aaaattgat tttgccaaa catttggag aaacaaagg gtgttccaa gctaactca aaatccaaa caaacggaa attttcgtt acgttacta cagtttgcc gattctaag cgtttcaac ttaccagtg acacaaaaa tcaatattt agcgatttc aatcaacat aaatatttg aaacaaggt gatatcagg agtatgttt gccaagccc aatgtcaat gctttgagc caagaacct gtaagccag aaattttct tttgggggc gaaaaagac gtgactcag tcgtttccg cctgaatta ttcgaagaa ttgtcaacg tttttatca atacaagac ttgtaccgt tgcaaaacg tgcgaattt ttattcgag tgtcctaca atcaaatat aacaaaact cgtgatact ttggatttg agttctttc acagttcct gatttatca gtagttgaa agtatcacc atcaaagat ttggaggtt tttgctgat tctttgagt ccaaaatcg agagaaaaa caccaagag ttgatggag gttgatgat agtttcgac gatattgat ttaagtgaa ctggaagaa atgtgtctg gaaaaaaat gtaccgtat gaaacccca cgaaacttt gattctttt atggattcg tttcgcata cgcgacgat caacaaatt gagaaagtt cccgaaaaa tcgtacatt tttgaacca cctgaaacg ttcaataaa gttttgaat ttattggaa ccgaaggaa gactataac gacgttttg gcatttttt aaactctca tcagttgct gaaattttc gaagaaact ggacacact cttgaaaag tgtgaagaa acaccggca gatttggat gaaactgtt atttacact cccgaaaag tgtgcaaac acctctcct gatattttc gaagggtca ccagattta ttcccttct gacgaaaaa caagacgtt tcattcgaa gcaccctta tcgccgatt ttatgcagc tacacaagg gtgcaagag ttaagtgcg aaaaagaaa ttgtttgag gaaaaaaaa ccgaattta gcaaacttg aaccaacga cgaaaaagt ttttttgaa accacaaat gctcaagtt tcgtcgccc aaaaagaat tttgatatt attagcgat atttgtgat ttatcgcag cttttgaat aatcgagaa gaaaacaag gaaaaagtt gagtctacg aaagtagat ttggatatt agtgatctt tgcgatttg tctgcgttt ggattgtcg cgaaaaaat gttgaaagc actttgcca aaggaagag gaaaagcca aaactggat ttatctatc gatgatttg tgcgacatg tcgcagttt tcagtcaaa aaagcggaa actcaaact acgagccat aaaaatatt gaagaacaa ctctcgagt cttgatgat ttttgcgat ttttcttgt ttccttcct gattcgaat aaagaaact cacaggaaa tcaatttcg gataagggg ataattaga ccacaaact cagaacttg aatgttagt acgattgat ttgactgag agtcccgtt aaagaacct tctaacaca gataataat aaatctcaa ctttcggta actcagatg ttaagttta atcgcgaaa agcgatagt cagaaagaa aatacgact ccaaaaacg aaaaaatcc cagcgttta acacaatca agacaaaaa tgtgagact cctaaatct acacaaaag aaaagttct cgagtaaag aaaagcccc aagtttaat atttcgttt gatggggac agtgaggac gagttcgag gaaaagagt ccaattttt gcaaaacct agaactatt tcactgaag aacaaaact gagaaaccg tcaaaaacg aaagagaga cgcaaatta gtgtgcgag tttctggat gaagaagcg gtcgtatct ggtgatgaa caaaccatt tcggatgat gaagatagt ggggaagat tgttttgaa gcaagtttt gtagctgat gaaacggag gatttgctt aatgataca ataatgcat gcgagatat ttacaatct gtgaaaagt ccagtgcga gggaagttc aaaattccg gacaaacct acgtttaat gtctcaaat gtgttctct caacaagtt agcaatgaa gatgacacg tatatgaac gattcgttc tgcgtgagc agccaagag gttgaaact tgcagcaac gacttgtcc gagctggaa ctactggaa atgcagctg aaggaggaa aaacaaaac aaaaaacga aaaaaatcg gacagtaaa agcgccgcg aaaaagcga agaatagtt gtgttggac gacagtgat taa
- >TC009341 RA
atcgtgatg ttgacaatt caaaaaact aacctcaca ctgcaatat tcaaacttg tttgttctt gttattctg ttcgtgatg taattataa tgaacactt cgcaaataa acacacaag gaatcaacg atgctggaa gcctgtgcc aggaccccg ccaccagag gcttcgact tacaagctg gccagacat ggatttacc ccacaaatc tcccaatcc gggagtacc aatacacta taaccgaaa aagccctcc actggaata ccctcgtaa gtcttccga caggtctcg gcaaaacct tcatcgcag ccgtggtca tgtacaact tctaccgat ggtaccccc atgacaagg tcattttca tggccccca cacgccccc tagtcaagc aacaaatgg acgcgtgct acaacatca tggccatcc ctcaggagg tcacagccg aactcactg gcaccaaaa tccagcaat cacgtaccg aaatctggc aaaataaac gagtttttt ttatcacac cgcaggttt tgcaaaacg atcttaaca taattgtcg agttggggc cgaaaatta agtgtttgg tgtttgatg aggcgcata gagcaaagg gtaatcacg cctattgtg aagttgtgc gtaagttat taccggaaa ataagttat ttcgggtgt tggcgttga gcgctacac ccgggaata gttcgaagg atgtgctgg atgttatga ggaatttgt tgattgcgc atttggaat tcaggagtg aggaaagtc ccgatgtga agccgtatg tgtttgaga gggcgcttg agactgttg tggtgccgt tgggtgaga agttgcaac aaatcaggg ataagtaca ttcaggttt tagaaaaat acaccagaa ccctaatcc agtataaag tgatttatg gaaattgtg ctaatctaa ccaaaggta aaatattca tggtgatga aggaattcc aggcgaaaa attcagcca atagaactg caaactatg cagaaatta tgaaatgtc taaacattt gtgtcacac tttatcatg cctatgaaa cccttgttc gttgtggtt tgagggcgt ttctgaact tttacaaag aacatatta ataatcctt tactacagg gaaatgtga gtataattc agataatgg acgaccttc aagcatact taggtccag accctgaag tccaaccac tgcccgatg gtacttttg ctgagattc cagcttcaa taaaatttg ggcacccga agttttacc aacttcgag acatccttg ttgcacatt tcacaaatt cggacgctt ccactcgtg ttattgtgt tttttgagt atcgggaga gcgccaccg aagcttacg ctttactca ctaggagtt ttcctctga taaggtccc gcgtttttg ttggtcaac gtgccggag tgacacaaa aagagcaaa taaatacgg tcaaaagtt tcagagaag gcacttgca ataccctcc tgtcgactt gtattggcg aagaaggcc ttgatgtgg gcgaagtcg acttgattg tttgcttcg atatagcga ataaatctc caattcgaa tgatacaaa gaatggggc ggaccgggc gtaaaaaag aagggcggg ttgtggttc tggttacgg aagggaggg aacaacaaa cgttgaaag attgtttga tttataaaa ataacttgg ggagttttg caacgaatt cgcaattgt tgcaagagg ggaaataca cagataatc ccaagatga taccttcgt acgtgcaac caaagtgcc aaaaaatgt gcatcactg ttaaaaaac ccgtcgtaa caaaaaccg ccaatttga aagatatgt tacgaaaca tttccgatg atttagaaa ttgttgaaa ttaaggaac gaatctctg ataagattg ataatttct ggaacaaaa ctagagaag aattcttcc aaatcgagg caaaaccca aaattgatt ttgccaaac atttggaga aacaaaggg tgttccaag ctaactcaa aatccaaac aaacggaaa ttttcgtta cgttactac agtttgccg attctaagc gtttcaact taccagtga cacaaaaat caatattta gcgatttca atcaacata aatatttga aacaaggtg atatcagga gtatgtttg ccaagccca atgtcaatg ctttgagcc aagaacctg taagccaga aattttctt ttgggggcg aaaaagacg tgactcagt cgtttccgc ctgaattat tcgaagaat tgtcaacgt ttttatcaa tacaagact tgtaccgtt gcaaaacgt gcgaatttt tattcgagt gtcctacaa tcaaatata acaaaactc gtgatactt tggatttga gttctttca cagttcctg atttatcag tagttgaaa gtatcacca tcaaagatt tggaggttt ttgctgatt ctttgagtc caaaatcga gagaaaaac accaagagt tgatggagg ttgatgata gtttcgacg atattgatt taagtgaac tggaagaaa tgtgtctgg aaaaaaatg taccgtatg aaaccccac gaaactttg attctttta tggattcgt ttcgcatac gcgacgatc aacaaattg agaaagttc ccgaaaaat cgtacattt ttgaaccac ctgaaacgt tcaataaag ttttgaatt tattggaac cgaaggaag actataacg acgttttgg catttttta aactctcat cagttgctg aaattttcg aagaaactg gacacactc ttgaaaagt gtgaagaaa caccggcag atttggatg aaactgtta tttacactc ccgaaaagt gtgcaaaca cctctcctg atattttcg aagggtcac cagatttat tcccttctg acgaaaaac aagacgttt cattcgaag cacccttat cgccgattt tatgcagct acacaaggg tgcaagagt taagtgcga aaaagaaat tgtttgagg aaaaaaaac cgaatttag caaacttga accaacgac gaaaaagtt tttttgaaa ccacaaatg ctcaagttt cgtcgccca aaaagaatt ttgatatta ttagcgata tttgtgatt tatcgcagc ttttgaata atcgagaag aaaacaagg aaaaagttg agtctacga aagtagatt tggatatta gtgatcttt gcgatttgt ctgcgtttg gattgtcgc gaaaaaatg ttgaaagca ctttgccaa aggaagagg aaaagccaa aactggatt tatctatcg atgatttgt gcgacatgt cgcagtttt cagtcaaaa aagcggaaa ctcaaacta cgagccata aaaatattg aagaacaac tctcgagtc ttgatgatt tttgcgatt tttcttgtt tccttcctg attcgaata aagaaactc acaggaaat caatttcgg ataagggga taattagac cacaaactc agaacttga atgttagta cgattgatt tgactgaga gtcccgtta aagaacctt ctaacacag ataataata aatctcaac tttcggtaa ctcagatgt taagtttaa tcgcgaaaa gcgatagtc agaaagaaa atacgactc caaaaacga aaaaatccc agcgtttaa cacaatcaa gacaaaaat gtgagactc ctaaatcta cacaaaaga aaagttctc gagtaaaga aaagcccca agtttaata tttcgtttg atggggaca gtgaggacg agttcgagg aaaagagtc caatttttg caaaaccta gaactattt cactgaaga acaaaactg agaaaccgt caaaaacga aagagagac gcaaattag tgtgcgagt ttctggatg aagaagcgg tcgtatctg gtgatgaac aaaccattt cggatgatg aagatagtg gggaagatt gttttgaag caagttttg tagctgatg aaacggagg atttgctta atgatacaa taatgcatg cgagatatt tacaatctg tgaaaagtc cagtgcgag ggaagttca aaattccgg acaaaccta cgtttaatg tctcaaatg tgttctctc aacaagtta gcaatgaag atgacacgt atatgaacg attcgttct gcgtgagca gccaagagg ttgaaactt gcagcaacg acttgtccg agctggaac tactggaaa tgcagctga aggaggaaa aacaaaaca aaaaacgaa aaaaatcgg acagtaaaa gcgccgcga aaaagcgaa gaatagttg tgttggacg acagtgatt aaataattt tttattaat aatcgttaa atattagtt taatgtcct caaaaaccg catcctctg acttgtttc catcgacga ctctcaaca aattcaatt ctctgtaat aatcaaccg ttggttgag tctccttaa ggtgtgtgg ccacccgtt tttcaataa cttcaatcg tgtcgtcaa aacgcccac ttccagccg cagcccctc tattgaggc acctctcaa tacaaacct ccacaggac aatcaataa ataaaacga acataactt caactttat ccccaattg tcctgctcc aaccttcgc gattttcca aattccgcg ggaatccgt caatgagaa aacggtctt gttgcgact cctccatgt gccgttcca gcaacttgc aagtgattt ctgccggaa ctatcttcc cgtctttta tacaagaat caatcagtt cgccgtgaa ccgaattgc ggacttgtc gctcacgcc tcagcaact caccggccg aaatgtgta caaaaccgt aataactgg ctattttac gacattgtg tccctttac caacccccg gcggcccca aaacaaaca caactttct tgtgcccca ttttcaagc cggtcggta acacccgtc cctaccaaa ccggccaag ccaaaacac ggaaaaaat cgaaaaatc gctttattg gcccgcgtt tgcaccaaa gatt
