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Gene information
General information
TC008438 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10270067..10295497
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008438
    iBeetle screen iB_07406

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008438 RA
      MDWAENSTL WNISQTLLN EVTTPETAT KTGSVPQWY 
      ETGRLTIPL YAVIFMLAV IGNTLVILT LVKNQRMRT 
      ITNLFLLNL AVSDLLLGV LCIPFTLIG TLLRHFVFG 
      EVMCKLLPF LQACSVSVG VWTLVAISV ERYYAICHP 
      LRSLRWQTI SHAYKLIVG IWIGSLICM APIALLSQL 
      KPTKQGNYK CREDWPSLD YEKAYNLFL DVVLLVIPL 
      LVLGVTYSL ITRTLCKGM KTERALRDH TVNGAVDVY 
      INLHGSSTT SRWSKRHNP NWRQLRHNW SQESSSPGT 
      GSQKYTPGL RRTNAERSL LNKKRVIKM LFAVVLEFF 
      ICWTPLYVI NTIVLFDSS VIYNNIGYK AITFFQLLA 
      YCSSCCNPI TYCFMNCGF RKSFLNLFK CLKKSRNFG 
      VTGSEINME TKWTNRCSE NADFCR
    CDS sequence
    • >TC008438 RA
      atggactgg gctgaaaac tccaccctc tggaacatt 
      tcacaaact ttattgaac gaagtgacg acaccggaa 
      accgcaacg aaaaccgga agtgtgcca caatggtac 
      gaaacgggc agacttacc atcccccta tacgccgtt 
      atctttatg ttggcagtc atcggaaac accctcgtt 
      atactcacc ttggtgaag aaccaaagg atgagaacg 
      ataacaaac ttgttcctc ctcaacttg gccgtttcg 
      gatcttctc ctaggcgtc ctttgcata cctttcacg 
      ttaataggg accctattg agacacttc gttttcggc 
      gaggtcatg tgcaagctt ctgcccttc ctccaagct 
      tgctccgtc agcgtcggt gtgtggact ctggtggcc 
      atttccgtc gagaggtac tacgcgatt tgtcaccct 
      ttgagatct ctccgatgg caaactatc agccacgct 
      tataagctc atagtcggg atatggatc ggaagtctc 
      atttgcatg gcacccatc gcccttttg agtcaactc 
      aagcccacc aagcaagga aattacaaa tgtcgcgaa 
      gattggccg tcactcgac tacgaaaaa gcctacaat 
      ttattctta gacgttgtc ctcctagtc atcccgcta 
      ctggtttta ggagtcact tactctctc ataacaagg 
      acactttgc aagggaatg aagacggaa agagcattg 
      cgcgaccac acagtgaac ggcgctgtg gacgtgtac 
      atcaacctc cacggctcg tccaccacc agccgttgg 
      agcaaacgc cacaacccc aattggcgc caactgcgc 
      cacaactgg tcgcaagaa tcctcaagt ccgggcacc 
      ggatcgcaa aaatacact ccgggactc cggcgaaca 
      aacgccgaa cgcagtctt ctcaacaag aagcgcgtc 
      ataaaaatg ctcttcgcc gtagttttg gagtttttc 
      atttgttgg acaccactc tacgtcatt aatacaatc 
      gtcttgttt gactcttcc gttatttac aacaacata 
      ggatataag gctataact ttctttcag ttgttggcg 
      tactgttcg agttgctgc aatcccata acttattgt 
      ttcatgaat tgtggattt cgaaagtcg tttttgaat 
      ctcttcaag tgtttgaaa aagtcgagg aacttcgga 
      gtgacgggc agcgagatt aacatggag acgaaatgg 
      accaatcgg tgctcggaa aatgccgac ttttgtaga 
      taa
    mRNA sequence
    • >TC008438 RA
      aaaatcaac aaaatcaac aaatcaaca ggtggcttt 
      gtcgcctga aaaattaat ttttcagca attaaggac 
      gaaattaat ttttcttat tgcaagtgt tggagcttt 
      cgggattat taaaatttc agaggcaag cttaaggta 
      gtcttaacc tatggcaat aatcggtgc aattgatgc 
      aggatatgg atcgtaggt caggataaa tcgtcttgt 
      gaatgaaag cgctggcgt cgctcggtc tgctcttga 
      aagctcaag cgtttcagt attcgttac gttttcaga 
      cggctatct tgaaactgt tgcttggat ttgatcgcg 
      cgcgctttt atgaccgtt taaaagtta cccaataaa 
      agaaaaaac ggcgtttcc aactaaaac gaccccaga 
      caccttcga ggtgatcga gaatgtaac aatggactg 
      ggctgaaaa ctccaccct ctggaacat ttcacaaac 
      tttattgaa cgaagtgac gacaccgga aaccgcaac 
      gaaaaccgg aagtgtgcc acaatggta cgaaacggg 
      cagacttac catccccct atacgccgt tatctttat 
      gttggcagt catcggaaa caccctcgt tatactcac 
      cttggtgaa gaaccaaag gatgagaac gataacaaa 
      cttgttcct cctcaactt ggccgtttc ggatcttct 
      cctaggcgt cctttgcat acctttcac gttaatagg 
      gaccctatt gagacactt cgttttcgg cgaggtcat 
      gtgcaagct tctgccctt cctccaagc ttgctccgt 
      cagcgtcgg tgtgtggac tctggtggc catttccgt 
      cgagaggta ctacgcgat ttgtcaccc tttgagatc 
      tctccgatg gcaaactat cagccacgc ttataagct 
      catagtcgg gatatggat cggaagtct catttgcat 
      ggcacccat cgccctttt gagtcaact caagcccac 
      caagcaagg aaattacaa atgtcgcga agattggcc 
      gtcactcga ctacgaaaa agcctacaa tttattctt 
      agacgttgt cctcctagt catcccgct actggtttt 
      aggagtcac ttactctct cataacaag gacactttg 
      caagggaat gaagacgga aagagcatt gcgcgacca 
      cacagtgaa cggcgctgt ggacgtgta catcaacct 
      ccacggctc gtccaccac cagccgttg gagcaaacg 
      ccacaaccc caattggcg ccaactgcg ccacaactg 
      gtcgcaaga atcctcaag tccgggcac cggatcgca 
      aaaatacac tccgggact ccggcgaac aaacgccga 
      acgcagtct tctcaacaa gaagcgcgt cataaaaat 
      gctcttcgc cgtagtttt ggagttttt catttgttg 
      gacaccact ctacgtcat taatacaat cgtcttgtt 
      tgactcttc cgttattta caacaacat aggatataa 
      ggctataac tttctttca gttgttggc gtactgttc 
      gagttgctg caatcccat aacttattg tttcatgaa 
      ttgtggatt tcgaaagtc gtttttgaa tctcttcaa 
      gtgtttgaa aaagtcgag gaacttcgg agtgacggg 
      cagcgagat taacatgga gacgaaatg gaccaatcg 
      gtgctcgga aaatgccga cttttgtag ataatttgt 
      gtgattatt ttgagcaga ataaacgga aattaaaaa 
      c
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