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Gene information
General information
TC006448 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9764582..9766171
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006448
    iBeetle screen iB_07399

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna unilateral not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006448 RA
      MLLKWSSVI EFNLFLLKW IGLWPGEDY QLNMYSFYG 
      FSVIILILC GHTLSTGLT LILDSGDID TFTETMFIL 
      NIEFMTAWK ALNFALNRK KFMQLLDAI DKTTFQPRN 
      GKQVTLVLR NIDGWKVMF KMFGISLGL SFIFTGLLP 
      IFSKTYKDR KLPMEAWYP FDSTKSPFY QLCYVYQMA 
      AVAVAVMVI LNVDTLVAA MNICIGLQC DLLCDNLRN 
      LHTNTSKMQ VFIYCWFGN EVIVKSSKI PYALFESDW 
      TQDSLEMKK NMIVFILRT QKTLKITVC HVFDLSLPT 
      FLTILKTGW SYFAFMNRV TSPH
    CDS sequence
    • >TC006448 RA
      atgctcttg aagtggtct tcggttatt gaatttaat 
      ctttttctt ctaaaatgg ataggactg tggcctgga 
      gaggactat caattaaat atgtattct ttctacggc 
      ttttctgtt attatattg atactttgt ggccacacc 
      ttatctaca ggcttgact ttaattttg gactcaggc 
      gacattgac acttttaca gaaacaatg tttatacta 
      aatattgaa tttatgaca gcgtggaaa gcgttaaat 
      tttgcatta aatcgaaaa aagttcatg cagttactt 
      gatgcaatt gataagaca acgtttcaa cccagaaac 
      ggcaaacaa gtaactttg gttttacga aatattgat 
      ggatggaaa gtaatgttc aagatgttt ggaatatct 
      cttggattg tctttcatt tttacgggt ttattaccg 
      atttttagt aaaacttac aaagaccgg aaattacca 
      atggaagcc tggtatcct tttgattca acaaagagt 
      ccattttac caattatgc tacgtgtat caaatggca 
      gctgttgca gttgcagtt atggttatt cttaatgtg 
      gatacgcta gttgctgcc atgaatatt tgcattggg 
      ctccagtgt gatttgttg tgtgataac ttgagaaac 
      cttcacact aacacttcg aaaatgcaa gttttcatc 
      tattgttgg tttggaaac gaagttata gttaagagt 
      agtaaaatt ccgtacgct ttatttgaa tctgactgg 
      actcaagat tcattggag atgaaaaag aatatgatt 
      gtgtttatt ttacgaaca caaaaaaca ttaaaaatc 
      accgtttgt cacgtgttt gatctttca ttaccaaca 
      ttcttaact attttgaaa actggatgg tcctacttt 
      gcttttatg aaccgggtc acgtcaccg cattag
    mRNA sequence
    • >TC006448 RA
      ctgaaagtg taaaaagct tggacatga gctttgagc 
      tttaagttg ctctacagt tttgtgtgc aaaatgctc 
      ttgaagtgg tcttcggtt attgaattt aatcttttt 
      cttctaaaa tggatagga ctgtggcct ggagaggac 
      tatcaatta aatatgtat tctttctac ggcttttct 
      gttattata ttgatactt tgtggccac accttatct 
      acaggcttg actttaatt ttggactca ggcgacatt 
      gacactttt acagaaaca atgtttata ctaaatatt 
      gaatttatg acagcgtgg aaagcgtta aattttgca 
      ttaaatcga aaaaagttc atgcagtta cttgatgca 
      attgataag acaacgttt caacccaga aacggcaaa 
      caagtaact ttggtttta cgaaatatt gatggatgg 
      aaagtaatg ttcaagatg tttggaata tctcttgga 
      ttgtctttc atttttacg ggtttatta ccgattttt 
      agtaaaact tacaaagac cggaaatta ccaatggaa 
      gcctggtat ccttttgat tcaacaaag agtccattt 
      taccaatta tgctacgtg tatcaaatg gcagctgtt 
      gcagttgca gttatggtt attcttaat gtggatacg 
      ctagttgct gccatgaat atttgcatt gggctccag 
      tgtgatttg ttgtgtgat aacttgaga aaccttcac 
      actaacact tcgaaaatg caagttttc atctattgt 
      tggtttgga aacgaagtt atagttaag agtagtaaa 
      attccgtac gctttattt gaatctgac tggactcaa 
      gattcattg gagatgaaa aagaatatg attgtgttt 
      attttacga acacaaaaa acattaaaa atcaccgtt 
      tgtcacgtg tttgatctt tcattacca acattctta 
      actattttg aaaactgga tggtcctac tttgctttt 
      atgaaccgg gtcacgtca ccgcattag aaaaataaa 
      aaaataata atgcttatc taaaattat tctaaaatg 
      ttataacaa ttttttttc ttatttaaa ggacgtcca 
      agaagggtt aatattgat gattttgag gtcaaacat 
      taaaaagtc tttaaatag caaacataa aaaatgtat 
      tttctgttt caaatgttc aaattaata aacatttca 
      actgcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY