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Gene information
General information
TC006095 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2381718..2391882
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006095
    iBeetle screen iB_07398

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006095 RA
      MSEKTSPLR YYLLNTSFH GFRYISEAG RHWTERVFW 
ALCCLLSWT ATLLLIKSS WEDFQTNAI SFVVDTSYL 
PWDTHFPSI SVCESDNQK RIAEITDRI YGDPHDYNL 
DEIVKELVY FRGLSFYTL QICGPEAQV PSEDCAKGD 
LGHFSQLVR SNCSQIFRK CRWNEEEFD CCAHFGALD 
TEMGVCYAI NSIQEDKTK RLKMVSNIK TGPGKLYLE 
INGFANVYI LGEQEVPSM TTLTTDVIQ VTPHIHYRR 
FLAVKEIDN QPEVKDVSI RQRKCRFSD ESDLNVYRY 
YSYSACCVQ CRKDAQLKI CNCAHHLMP NTPPKNKCN 
ITGLYCLSK HYNELSVLK PYWANRTGL VCDCLPSCT 
EMELAVVRD DKIGIPEDY AIVELALER LPTERYKRN 
VVRGKLDLV VSMGGSTAL FLGASILSF VEVLYYFFV 
RPVSDSLIT KQKMKENQK FRRFKDQSR LPTIS
    CDS sequence
    • >TC006095 RA
      atgagcgaa aaaacatca cccttacga tattacctc 
ctcaatact tcgtttcat ggctttcgg tatatttcg 
gaagctgga aggcactgg actgagcgc gttttttgg 
gccctttgc tgcctcctc agctggacc gccaccctc 
ctcctgatc aaatcctcc tgggaggac ttccaaacc 
aacgccatc agcttcgtg gtggacacc agctacctc 
ccctgggac acccacttc ccgtccatc tccgtgtgc 
gaaagcgac aaccagaaa cgcatcgcc gaaatcacc 
gaccgcatc tacggcgac ccccacgac tacaacctc 
gacgaaatc gtcaaagaa ctagtctac ttccgcggc 
ctctccttc tacaccctg caaatctgc ggccccgag 
gcccaggtc ccgagcgag gactgcgcc aagggcgac 
ctggggcac ttctcccag ctcgtccga agcaactgc 
agccagatt ttccgcaag tgtagatgg aacgaggag 
gagttcgac tgctgtgcg cactttggg gcgctcgac 
accgaaatg ggcgtctgc tacgccatt aattccatc 
caggaggac aagaccaag aggctgaag atggtgtcc 
aacatcaaa acggggccc gggaagttg tacctggag 
atcaacggg ttcgcgaat gtgtacatc ctgggggag 
caggaggtg ccctccatg accacgctg accacggac 
gtgatccag gtcacgcca catatccac tacaggagg 
tttctggcc gtcaaggaa attgataat caaccagaa 
gtgaaagat gtgagcatt cgccaacgc aagtgccgt 
ttttcggac gaatccgat ttaaacgtt tatcgctac 
tatagctat agcgcttgt tgcgtgcag tgtcggaaa 
gacgcacag ctcaaaatt tgcaattgt gcccaccat 
ttaatgccc aacacacca ccaaagaac aaatgtaac 
attacgggc ctgtattgt ttaagcaaa cattacaat 
gaattgtcc gttttgaag ccgtattgg gccaacagg 
accggactt gtgtgcgac tgcctgccc agctgtacc 
gagatggag ttggctgtg gtcagggat gataagatt 
ggcattcct gaagattac gctattgtg gaactggcg 
ttagaacgt ttaccaact gagcgctac aaaaggaac 
gtcgttaga gggaaatta gatctagtc gtgtcaatg 
ggtggcagc actgccctt tttttgggc gccagcatt 
ttaagtttc gtcgaagtg ttgtactac tttttcgtg 
cgccctgtc agcgactcg cttataacc aaacaaaaa 
atgaaagaa aaccaaaaa ttccgccgt ttcaaggac 
caatcaaga ctcccaaca atttcataa
    mRNA sequence
    • >TC006095 RA
      taggacaac aaaaggtgg tgatgataa cggaaatta 
tcgtatttt acttctttt agttggaaa atgggataa 
tttatattg atttagcaa ttacttcaa ataaaagct 
gatacacct attggaaaa attgtagta acgtttatt 
cagtgattt caaaatgag cgaaaaaac atcaccctt 
acgatatta cctcctcaa tacttcgtt tcatggctt 
tcggtatat ttcggaagc tggaaggca ctggactga 
gcgcgtttt ttgggccct ttgctgcct cctcagctg 
gaccgccac cctcctcct gatcaaatc ctcctggga 
ggacttcca aaccaacgc catcagctt cgtggtgga 
caccagcta cctcccctg ggacaccca cttcccgtc 
catctccgt gtgcgaaag cgacaacca gaaacgcat 
cgccgaaat caccgaccg catctacgg cgaccccca 
cgactacaa cctcgacga aatcgtcaa agaactagt 
ctacttccg cggcctctc cttctacac cctgcaaat 
ctgcggccc cgaggccca ggtcccgag cgaggactg 
cgccaaggg cgacctggg gcacttctc ccagctcgt 
ccgaagcaa ctgcagcca gattttccg caagtgtag 
atggaacga ggaggagtt cgactgctg tgcgcactt 
tggggcgct cgacaccga aatgggcgt ctgctacgc 
cattaattc catccagga ggacaagac caagaggct 
gaagatggt gtccaacat caaaacggg gcccgggaa 
gttgtacct ggagatcaa cgggttcgc gaatgtgta 
catcctggg ggagcagga ggtgccctc catgaccac 
gctgaccac ggacgtgat ccaggtcac gccacatat 
ccactacag gaggtttct ggccgtcaa ggaaattga 
taatcaacc agaagtgaa agatgtgag cattcgcca 
acgcaagtg ccgtttttc ggacgaatc cgatttaaa 
cgtttatcg ctactatag ctatagcgc ttgttgcgt 
gcagtgtcg gaaagacgc acagctcaa aatttgcaa 
ttgtgccca ccatttaat gcccaacac accaccaaa 
gaacaaatg taacattac gggcctgta ttgtttaag 
caaacatta caatgaatt gtccgtttt gaagccgta 
ttgggccaa caggaccgg acttgtgtg cgactgcct 
gcccagctg taccgagat ggagttggc tgtggtcag 
ggatgataa gattggcat tcctgaaga ttacgctat 
tgtggaact ggcgttaga acgtttacc aactgagcg 
ctacaaaag gaacgtcgt tagagggaa attagatct 
agtcgtgtc aatgggtgg cagcactgc ccttttttt 
gggcgccag cattttaag tttcgtcga agtgttgta 
ctacttttt cgtgcgccc tgtcagcga ctcgcttat 
aaccaaaca aaaaatgaa agaaaacca aaaattccg 
ccgtttcaa ggaccaatc aagactccc aacaatttc 
ataaacttt tattcaagc atctctcgc aatttctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY