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Gene information
General information
TC004958 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5156574..5259559
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004958
    iBeetle screen iB_07396

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004958 RA
      MARKAPFWR GFGLLLVPI TSLLVTSLQ AFQEDWSTA 
      RISDEPEFL APIHNLTTP VGREATLSC TVSNLGKYK 
      VAWVRAEDQ TILTLHTKV VTHNSRISV THDNLKTWH 
      LRIRQLKES DRGCYMCQI NTSVMKIQQ GCLDVYVPP 
      DIINDDTSG DLSVSEGEN ATLWCRATG HPTPRIAWK 
      REDSKPIIL RKGPRQEER VETYNGTNL HFWRLDRKQ 
      MGAYLCIAS NDVPPAVSK RIALNVNFA PSIKVPNQL 
      LGAPLSTDV HLECYVEAF PNTINYWVK NRGEMLLNG 
      SKYTISETR RGYKVSMQL VIHNFTVPD IGTYNCVST 
      NSLGRAEGT LRLYGQ
    • >TC004958 RB
      MFISEDWST ARISDEPEF LAPIHNLTT PVGREATLS 
      CTVSNLGKY KVAWVRAED QTILTLHTK VVTHNSRIS 
      VTHDNLKTW HLRIRQLKE SDRGCYMCQ INTSVMKIQ 
      QGCLDVYVP PDIINDDTS GDLSVSEGE NATLWCRAT 
      GHPTPRIAW KREDSKPII LRKGPRQEE RVETYNGTN 
      LHFWRLDRK QMGAYLCIA SNDVPPAVS KRIALNVNF 
      APSIKVPNQ LLGAPLSTD VHLECYVEA FPNTINYWV 
      KNRGEMLLN GSKYTISET RRGYKVSMQ LVIHNFTVP 
      DIGTYNCVS TNSLGRAEG TLRLYEIKV GPGLPHDNH 
      VSIVGGLAE PARGHRSRG NDAFSSAVL TRPLLVALT 
      ATSLLSSMS LR
    • >TC004958 RC
      MFISEDWST ARISDEPEF LAPIHNLTT PVGREATLS 
      CTVSNLGKY KVAWVRAED QTILTLHTK VVTHNSRIS 
      VTHDNLKTW HLRIRQLKE SDRGCYMCQ INTSVMKIQ 
      QGCLDVYVP PDIINDDTS GDLSVSEGE NATLWCRAT 
      GHPTPRIAW KREDSKPII LRKGPRQEE RVETYNGTN 
      LHFWRLDRK QMGAYLCIA SNDVPPAVS KRIALNVNF 
      APSIKVPNQ LLGAPLSTD VHLECYVEA FPNTINYWV 
      KNRGEMLLN GSKYTISET RRGYKVSMQ LVIHNFTVP 
      DIGTYNCVS TNSLGRAEG TLRLYGQ
    • >TC004958 RD
      MTQFCFAEI KVGPGLPHD NHVSIVGGL AEPARGHRS 
      RGNDAFSSA VLTRPLLVA LTATSLLSS MSLR
    CDS sequence
    • >TC004958 RA
      atggcgaga aaagctcct ttctggaga gggttcgga 
      ctccttctt gttcctata acatcgctc cttgttacg 
      tcccttcag gcctttcaa gaggactgg tcgactgct 
      cggatatca gatgaaccc gagttttta gctcctata 
      cataattta acaactccg gtgggaaga gaggctaca 
      ctttcttgc acagtcagc aacctcgga aaatacaag 
      gtggcttgg gtccgtgct gaggaccaa acaatttta 
      actcttcac acgaaggtt gtgactcac aactcccga 
      atcagtgtg acccatgac aatctaaag acgtggcat 
      ttgaggatt cgccaattg aaagaatcg gatcgtggg 
      tgttacatg tgtcagatc aacaccagc gtcatgaaa 
      attcaacag gggtgtctg gacgtttat gttcctccc 
      gacataatc aatgacgac acgagtggg gacttgtct 
      gtgtctgaa ggtgagaat gcaaccctt tggtgtcga 
      gcaactggt catccaaca cctcgaatc gcttggaaa 
      cgagaagac tccaaaccc attatcctc cgtaaaggc 
      ccgagacag gaagaaaga gtggagact tacaatggg 
      acgaatcta catttttgg aggctggat cggaaacaa 
      atgggtgct tatctgtgt atcgcaagc aacgatgtg 
      ccaccagca gtcagtaaa agaatcgca ctcaacgta 
      aattttgcg ccctccatt aaagtgcca aatcagctc 
      ctcggcgca cctcttagc acagacgtc cacctcgaa 
      tgttacgtc gaagctttc cctaacaca atcaactac 
      tgggtaaaa aaccgggga gagatgttg ctgaacggg 
      agcaaatac acgatatcc gagacccgc cgcggctac 
      aaggtctcc atgcagctc gtcatccac aattttaca 
      gtacctgac ataggcacc tacaattgt gtttcaacg 
      aactctctg ggacgagcc gaaggcacc ctccgacta 
      tacggtcag taa
    • >TC004958 RB
      atgtttatt tcagaggac tggtcgact gctcggata 
      tcagatgaa cccgagttt ttagctcct atacataat 
      ttaacaact ccggtggga agagaggct acactttct 
      tgcacagtc agcaacctc ggaaaatac aaggtggct 
      tgggtccgt gctgaggac caaacaatt ttaactctt 
      cacacgaag gttgtgact cacaactcc cgaatcagt 
      gtgacccat gacaatcta aagacgtgg catttgagg 
      attcgccaa ttgaaagaa tcggatcgt gggtgttac 
      atgtgtcag atcaacacc agcgtcatg aaaattcaa 
      caggggtgt ctggacgtt tatgttcct cccgacata 
      atcaatgac gacacgagt ggggacttg tctgtgtct 
      gaaggtgag aatgcaacc ctttggtgt cgagcaact 
      ggtcatcca acacctcga atcgcttgg aaacgagaa 
      gactccaaa cccattatc ctccgtaaa ggcccgaga 
      caggaagaa agagtggag acttacaat gggacgaat 
      ctacatttt tggaggctg gatcggaaa caaatgggt 
      gcttatctg tgtatcgca agcaacgat gtgccacca 
      gcagtcagt aaaagaatc gcactcaac gtaaatttt 
      gcgccctcc attaaagtg ccaaatcag ctcctcggc 
      gcacctctt agcacagac gtccacctc gaatgttac 
      gtcgaagct ttccctaac acaatcaac tactgggta 
      aaaaaccgg ggagagatg ttgctgaac gggagcaaa 
      tacacgata tccgagacc cgccgcggc tacaaggtc 
      tccatgcag ctcgtcatc cacaatttt acagtacct 
      gacataggc acctacaat tgtgtttca acgaactct 
      ctgggacga gccgaaggc accctccga ctatacgaa 
      attaaggtg ggtccaggc ttgccacac gacaaccac 
      gtgagcata gttggtggt ttggccgaa ccggctcga 
      ggtcaccgt tcaaggggc aacgatgcc ttctcctca 
      gccgttctg acacgtcca ctcctcgta gccctaacg 
      gccacttca ctcctaagt tcgatgtct ttgcggtga
    • >TC004958 RC
      atgtttatt tcagaggac tggtcgact gctcggata 
      tcagatgaa cccgagttt ttagctcct atacataat 
      ttaacaact ccggtggga agagaggct acactttct 
      tgcacagtc agcaacctc ggaaaatac aaggtggct 
      tgggtccgt gctgaggac caaacaatt ttaactctt 
      cacacgaag gttgtgact cacaactcc cgaatcagt 
      gtgacccat gacaatcta aagacgtgg catttgagg 
      attcgccaa ttgaaagaa tcggatcgt gggtgttac 
      atgtgtcag atcaacacc agcgtcatg aaaattcaa 
      caggggtgt ctggacgtt tatgttcct cccgacata 
      atcaatgac gacacgagt ggggacttg tctgtgtct 
      gaaggtgag aatgcaacc ctttggtgt cgagcaact 
      ggtcatcca acacctcga atcgcttgg aaacgagaa 
      gactccaaa cccattatc ctccgtaaa ggcccgaga 
      caggaagaa agagtggag acttacaat gggacgaat 
      ctacatttt tggaggctg gatcggaaa caaatgggt 
      gcttatctg tgtatcgca agcaacgat gtgccacca 
      gcagtcagt aaaagaatc gcactcaac gtaaatttt 
      gcgccctcc attaaagtg ccaaatcag ctcctcggc 
      gcacctctt agcacagac gtccacctc gaatgttac 
      gtcgaagct ttccctaac acaatcaac tactgggta 
      aaaaaccgg ggagagatg ttgctgaac gggagcaaa 
      tacacgata tccgagacc cgccgcggc tacaaggtc 
      tccatgcag ctcgtcatc cacaatttt acagtacct 
      gacataggc acctacaat tgtgtttca acgaactct 
      ctgggacga gccgaaggc accctccga ctatacggt 
      cagtaa
    • >TC004958 RD
      atgacacaa ttttgtttt gcagaaatt aaggtgggt 
      ccaggcttg ccacacgac aaccacgtg agcatagtt 
      ggtggtttg gccgaaccg gctcgaggt caccgttca 
      aggggcaac gatgccttc tcctcagcc gttctgaca 
      cgtccactc ctcgtagcc ctaacggcc acttcactc 
      ctaagttcg atgtctttg cggtga
    mRNA sequence
    • >TC004958 RA
      gagtttcag tgcaattaa ctattttat cgcagcggc 
      cattccgag tgattcacc ggttgctga aagtcccgg 
      attatcttc gacaaaaac gttataatt ttgttccgg 
      gtaacagtt ttcatttga gcggataaa tcagaaacc 
      acttgttac tgctgattt atgtcgagg actggaact 
      gcaaacaaa gtagttgtg gggaaagat tccgatcga 
      tcgttgata tctaccgga gtgcatgtt tttatgtct 
      cgtgacata ttacgagtg tttcggatt atttagaat 
      gtaaggacg ggaagaagt gctcgaacg atggcgaga 
      aaagctcct ttctggaga gggttcgga ctccttctt 
      gttcctata acatcgctc cttgttacg tcccttcag 
      gcctttcaa gaggactgg tcgactgct cggatatca 
      gatgaaccc gagttttta gctcctata cataattta 
      acaactccg gtgggaaga gaggctaca ctttcttgc 
      acagtcagc aacctcgga aaatacaag gtggcttgg 
      gtccgtgct gaggaccaa acaatttta actcttcac 
      acgaaggtt gtgactcac aactcccga atcagtgtg 
      acccatgac aatctaaag acgtggcat ttgaggatt 
      cgccaattg aaagaatcg gatcgtggg tgttacatg 
      tgtcagatc aacaccagc gtcatgaaa attcaacag 
      gggtgtctg gacgtttat gttcctccc gacataatc 
      aatgacgac acgagtggg gacttgtct gtgtctgaa 
      ggtgagaat gcaaccctt tggtgtcga gcaactggt 
      catccaaca cctcgaatc gcttggaaa cgagaagac 
      tccaaaccc attatcctc cgtaaaggc ccgagacag 
      gaagaaaga gtggagact tacaatggg acgaatcta 
      catttttgg aggctggat cggaaacaa atgggtgct 
      tatctgtgt atcgcaagc aacgatgtg ccaccagca 
      gtcagtaaa agaatcgca ctcaacgta aattttgcg 
      ccctccatt aaagtgcca aatcagctc ctcggcgca 
      cctcttagc acagacgtc cacctcgaa tgttacgtc 
      gaagctttc cctaacaca atcaactac tgggtaaaa 
      aaccgggga gagatgttg ctgaacggg agcaaatac 
      acgatatcc gagacccgc cgcggctac aaggtctcc 
      atgcagctc gtcatccac aattttaca gtacctgac 
      ataggcacc tacaattgt gtttcaacg aactctctg 
      ggacgagcc gaaggcacc ctccgacta tacggtcag 
      taaaaagca atcttatcg actgtgtac ctccattcc 
      gttcccggc tttgttcac tcacttgtc ctcattgaa
    • >TC004958 RB
      cttttttat ttttttata ttagttttt atatattta 
      ttcttcaac actgtcaaa atttacggt ttttctcct 
      gtgtaagga agttttgac aactgtttg gcacttctc 
      agtacgaaa ccgcaaatc tgaattctc gtcgatgca 
      agtgttaat gtaatttat tctatgttt atttcagag 
      gactggtcg actgctcgg atatcagat gaacccgag 
      tttttagct cctatacat aatttaaca actccggtg 
      ggaagagag gctacactt tcttgcaca gtcagcaac 
      ctcggaaaa tacaaggtg gcttgggtc cgtgctgag 
      gaccaaaca attttaact cttcacacg aaggttgtg 
      actcacaac tcccgaatc agtgtgacc catgacaat 
      ctaaagacg tggcatttg aggattcgc caattgaaa 
      gaatcggat cgtgggtgt tacatgtgt cagatcaac 
      accagcgtc atgaaaatt caacagggg tgtctggac 
      gtttatgtt cctcccgac ataatcaat gacgacacg 
      agtggggac ttgtctgtg tctgaaggt gagaatgca 
      accctttgg tgtcgagca actggtcat ccaacacct 
      cgaatcgct tggaaacga gaagactcc aaacccatt 
      atcctccgt aaaggcccg agacaggaa gaaagagtg 
      gagacttac aatgggacg aatctacat ttttggagg 
      ctggatcgg aaacaaatg ggtgcttat ctgtgtatc 
      gcaagcaac gatgtgcca ccagcagtc agtaaaaga 
      atcgcactc aacgtaaat tttgcgccc tccattaaa 
      gtgccaaat cagctcctc ggcgcacct cttagcaca 
      gacgtccac ctcgaatgt tacgtcgaa gctttccct 
      aacacaatc aactactgg gtaaaaaac cggggagag 
      atgttgctg aacgggagc aaatacacg atatccgag 
      acccgccgc ggctacaag gtctccatg cagctcgtc 
      atccacaat tttacagta cctgacata ggcacctac 
      aattgtgtt tcaacgaac tctctggga cgagccgaa 
      ggcaccctc cgactatac gaaattaag gtgggtcca 
      ggcttgcca cacgacaac cacgtgagc atagttggt 
      ggtttggcc gaaccggct cgaggtcac cgttcaagg 
      ggcaacgat gccttctcc tcagccgtt ctgacacgt 
      ccactcctc gtagcccta acggccact tcactccta 
      agttcgatg tctttgcgg tgacgcacc taaattcag 
      tgttttagt gcatcgccg taacatttc atccatcca 
      tcattcgaa aaacttgcc acgaaagaa cctttcagg 
      ctcctaata cgaatacgg atcaatcaa accgcgaac 
      tctttcctt ccgacgtta ttgcagcgt gaacgcttt 
      tgttttgtt gtgcaacat tttgagaca aaaaataca 
      acgaattca gccaaaaat cgagtgcga gaggctatg 
      tttgccgat accatcatg tttaattaa tcagtggtg 
      atcaaaaga tgtttttgt tgttagtta cgtttaccc 
      tccacttcg gacgcaaga aacgtgaat gttaccaac 
      tagccaact aattaatta ccttgctaa caattaaat 
      aaccatgtt ccatcactt acaacacgg tttttcaac 
      ccaatcaag tgatggagt ggcgcagtg aatgtctgt 
      agtttagtt tgatttttg tcttgaaat gttacgttt 
      taaagaagg caaaacaca gttttgtgc ttctttaaa 
      caatacttg agtcaattt ataaaatta gaactcaca 
      gtcccattt tgaaagtac tattttttt aaatcaact 
      gtaacatat tgtaaatac gatcggtgt aggtttatt 
      atttcctaa agttttgta aaatactgt acataagta 
      atactcaag gtgatagaa acttatttg atatgaaaa 
      ctgtaatta gttttgtgt ataattcat aactcgcat 
      ttgtaaaat cattagatt cctaaaaat cgaaattag 
      tattcatag gtattatgt attcgaatg gtgccaaat 
      caaaataat atagaataa atgtacgca aatata
    • >TC004958 RC
      cttttttat ttttttata ttagttttt atatattta 
      ttcttcaac actgtcaaa atttacggt ttttctcct 
      gtgtaagga agttttgac aactgtttg gcacttctc 
      agtacgaaa ccgcaaatc tgaattctc gtcgatgca 
      agtgttaat gtaatttat tctatgttt atttcagag 
      gactggtcg actgctcgg atatcagat gaacccgag 
      tttttagct cctatacat aatttaaca actccggtg 
      ggaagagag gctacactt tcttgcaca gtcagcaac 
      ctcggaaaa tacaaggtg gcttgggtc cgtgctgag 
      gaccaaaca attttaact cttcacacg aaggttgtg 
      actcacaac tcccgaatc agtgtgacc catgacaat 
      ctaaagacg tggcatttg aggattcgc caattgaaa 
      gaatcggat cgtgggtgt tacatgtgt cagatcaac 
      accagcgtc atgaaaatt caacagggg tgtctggac 
      gtttatgtt cctcccgac ataatcaat gacgacacg 
      agtggggac ttgtctgtg tctgaaggt gagaatgca 
      accctttgg tgtcgagca actggtcat ccaacacct 
      cgaatcgct tggaaacga gaagactcc aaacccatt 
      atcctccgt aaaggcccg agacaggaa gaaagagtg 
      gagacttac aatgggacg aatctacat ttttggagg 
      ctggatcgg aaacaaatg ggtgcttat ctgtgtatc 
      gcaagcaac gatgtgcca ccagcagtc agtaaaaga 
      atcgcactc aacgtaaat tttgcgccc tccattaaa 
      gtgccaaat cagctcctc ggcgcacct cttagcaca 
      gacgtccac ctcgaatgt tacgtcgaa gctttccct 
      aacacaatc aactactgg gtaaaaaac cggggagag 
      atgttgctg aacgggagc aaatacacg atatccgag 
      acccgccgc ggctacaag gtctccatg cagctcgtc 
      atccacaat tttacagta cctgacata ggcacctac 
      aattgtgtt tcaacgaac tctctggga cgagccgaa 
      ggcaccctc cgactatac ggtcagtaa aaagcaatc 
      ttatcgact gtgtacctc cattccgtt cccggcttt 
      gttcactca cttgtcctc attgaa
    • >TC004958 RD
      aattgccaa aatgacaca attttgttt tgcagaaat 
      taaggtggg tccaggctt gccacacga caaccacgt 
      gagcatagt tggtggttt ggccgaacc ggctcgagg 
      tcaccgttc aaggggcaa cgatgcctt ctcctcagc 
      cgttctgac acgtccact cctcgtagc cctaacggc 
      cacttcact cctaagttc gatgtcttt gcggtgacg 
      cacctaaat tcagtgttt tagtgcatc gccgtaaca 
      tttcatcca tccatcatt cgaaaaact tgccacgaa 
      agaaccttt caggctcct aatacgaat acggatcaa 
      tcaaaccgc gaactcttt ccttccgac gttattgca 
      gcgtgaacg cttttgttt tgttgtgca acattttga 
      gacaaaaaa tacaacgaa ttcagccaa aaatcgagt 
      gcgagaggc tatgtttgc cgataccat catgtttaa 
      ttaatcagt ggtgatcaa aagatgttt ttgttgtta 
      gttacgttt accctccac ttcggacgc aagaaacgt 
      gaatgttac caactagcc aactaatta attaccttg 
      ctaacaatt aaataacca tgttccatc acttacaac 
      acggttttt caacccaat caagtgatg gagtggcgc 
      agtgaatgt ctgtagttt agtttgatt tttgtcttg 
      aaatgttac gttttaaag aaggcaaaa cacagtttt 
      gtgcttctt taaacaata cttgagtca atttataaa 
      attagaact cacagtccc attttgaaa gtactattt 
      ttttaaatc aactgtaac atattgtaa atacgatcg 
      gtgtaggtt tattatttc ctaaagttt tgtaaaata 
      ctgtacata agtaatact caaggtgat agaaactta 
      tttgatatg aaaactgta attagtttt gtgtataat 
      tcataactc gcatttgta aaatcatta gattcctaa 
      aaatcgaaa ttagtattc ataggtatt atgtattcg 
      aatggtgcc aaatcaaaa taatataga ataaatgta 
      cgcaaatat a
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