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Gene information
General information
TC003780 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17658191..17659678
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003780
    iBeetle screen iB_07393

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003780 RA
      MKAQDSDNP YIVLRRVFV DFAFTSHMI IYTKITFVF 
      HFLTLLLET YYMITNFNV ELFSRYGCM MCLMTYIVL 
      AKLLEILFA RHIKFLEEE RLSHFWKLE ESSEETQKV 
      VNAESSKIR KKTFFVLSW FVALGFVLF PIFGDLNDL 
      FMFGRVYRN YFGSWAIIP FCIYVSTFP SIAYNSICL 
      PAVVSYFIF HLNLQISLI NDKLGKISE KSRQSEIYQ 
      KLCSCVAHH VRLRRWTNI FQNELESAL PFYLFLGAI 
      NSIAVSFFI LYNLQNMTL IFEIRLVVI SVCNVLILW 
      IFAEAGQEF SDNSDSIFD AVVACPWYS WNAQNRKIM 
      LIFMLNCLK PMTFSWGGV KLDYQFTVT IVKMSYSYA 
      LVLYNWRYE K
    CDS sequence
    • >TC003780 RA
      atgaaagct caagactct gacaaccct tatatcgtt 
      ctgagacgc gtttttgtc gattttgct ttcactagc 
      cacatgata atctacact aaaatcact ttcgtgttt 
      cacttcttg actttactc ctcgaaact tattacatg 
      attacaaac tttaatgtg gaactcttt tcacgttat 
      ggttgcatg atgtgcctc atgacttat atcgttttg 
      gctaaacta ttggaaatt ttatttgcc agacatatc 
      aaatttttg gaggaagag cgtctttct catttctgg 
      aaacttgaa gaaagtagt gaagaaaca caaaaagtg 
      gtgaacgcg gaatcttca aaaattagg aaaaaaacg 
      ttttttgtt ctatcgtgg tttgtggcg ttagggttt 
      gttttgttc ccaattttt ggggatttg aacgatttg 
      ttcatgttt ggtcgagtg tataggaat tattttgga 
      agttgggcc attattccc ttctgtatt tacgtttcc 
      acgtttcct tcgatcgct tacaactct atttgttta 
      ccagcagtt gtatcatat tttatattt cacctaaac 
      ttacagata agcttaata aatgacaaa cttggcaaa 
      atttcagaa aaatcccgc cagagcgag atttaccaa 
      aaattatgc tcatgtgtt gctcaccac gtgaggcta 
      agaagatgg acaaatata tttcaaaat gagttggaa 
      tcagctctg cctttttac ctcttcttg ggtgctatt 
      aattctatt gctgtctca tttttcatt ctttataat 
      cttcaaaac atgactcta atttttgaa atacgtttg 
      gtcgtaatt tcagtctgt aatgttctg attctttgg 
      atttttgct gaagctgga caagaattt agtgataat 
      tctgactca atttttgat gctgtagta gcatgtcct 
      tggtacagt tggaacgct caaaatcgg aaaattatg 
      ttgatattt atgctgaat tgtttaaaa ccaatgact 
      ttttcgtgg ggcggcgtt aaactcgac tatcaattt 
      actgtaact atcgtcaaa atgtcttac tcttacgct 
      ttggttttg tataactgg agatatgaa aagtag
    mRNA sequence
    • >TC003780 RA
      ttttactac tgaaacacc tcacaatga aagctcaag 
      actctgaca acccttata tcgttctga gacgcgttt 
      ttgtcgatt ttgctttca ctagccaca tgataatct 
      acactaaaa tcactttcg tgtttcact tcttgactt 
      tactcctcg aaacttatt acatgatta caaacttta 
      atgtggaac tcttttcac gttatggtt gcatgatgt 
      gcctcatga cttatatcg ttttggcta aactattgg 
      aaattttat ttgccagac atatcaaat ttttggagg 
      aagagcgtc tttctcatt tctggaaac ttgaagaaa 
      gtagtgaag aaacacaaa aagtggtga acgcggaat 
      cttcaaaaa ttaggaaaa aaacgtttt ttgttctat 
      cgtggtttg tggcgttag ggtttgttt tgttcccaa 
      tttttgggg atttgaacg atttgttca tgtttggtc 
      gagtgtata ggaattatt ttggaagtt gggccatta 
      ttcccttct gtatttacg tttccacgt ttccttcga 
      tcgcttaca actctattt gtttaccag cagttgtat 
      catatttta tatttcacc taaacttac agataagct 
      taataaatg acaaacttg gcaaaattt cagaaaaat 
      cccgccaga gcgagattt accaaaaat tatgctcat 
      gtgttgctc accacgtga ggctaagaa gatggacaa 
      atatatttc aaaatgagt tggaatcag ctctgcctt 
      tttacctct tcttgggtg ctattaatt ctattgctg 
      tctcatttt tcattcttt ataatcttc aaaacatga 
      ctctaattt ttgaaatac gtttggtcg taatttcag 
      tctgtaatg ttctgattc tttggattt ttgctgaag 
      ctggacaag aatttagtg ataattctg actcaattt 
      ttgatgctg tagtagcat gtccttggt acagttgga 
      acgctcaaa atcggaaaa ttatgttga tatttatgc 
      tgaattgtt taaaaccaa tgacttttt cgtggggcg 
      gcgttaaac tcgactatc aatttactg taactatcg 
      tcaaaatgt cttactctt acgctttgg ttttgtata 
      actggagat atgaaaagt agcaagtta actgctttg 
      taaccatca ctattttga aaaacatac agtttaacg 
      aaatttgtt gaaaaattt taatttatt attttttta 
      gagaattta ataaaatat tttagtggg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY