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Gene information
General information
TC003219 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11..3097
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003219
    iBeetle screen iB_07389

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003219 RA
      MWSLLPVLN GWTWQKKLP FPARYPLDV 
      TKSPYYELA YVYQFICIW YITVANLNL 
      DTIIIALMM YTSCQCDLL CDDLKNLTE 
      TRFFDKKLI ECIKHHKAI LVFAEKSNS 
      LFNMIVLGQ IATSTVVLA LTMFQLSMV 
      SPLSSEGLN HLFYIGGII MQILLYCWF 
      GNEVEAKSS NILYAIYES TWSEASKNS 
      KKNLIIFSI RCQRPIKAT AVKLFALSL 
      RTFITIVRS GWSYFAVLY NVGSE
    CDS sequence
    • >TC003219 RA
      atgtggtcg ctacttcca gtcttgaat ggatggact 
      tggcaaaag aaattgcct tttccagct cgttatccg 
      ctcgatgtt acaaaatcg ccgtattat gaactagcc 
      tatgtttac caattcatc tgtatttgg tacataact 
      gttgccaat ttaaattta gatacaatt atcatagcg 
      ttaatgatg tatacatcg tgtcagtgt gaccttttg 
      tgcgacgat ttaaaaaat cttacagag acgcgattt 
      tttgataaa aaactgatt gaatgtatt aagcatcat 
      aaagctatt ttagttttt gcagaaaaa agtaatagt 
      ttatttaat atgattgtc ttgggtcaa attgccaca 
      agcaccgtg gttctggcc ttgacaatg ttccagttg 
      agcatggta agccctttg agcagtgaa ggtttgaat 
      catctcttc tacattgga ggtattata atgcaaatt 
      ctgctttac tgttggttt ggaaatgaa gttgaagca 
      aagagcagc aatatttta tacgcaatt tatgaatcc 
      acttggtct gaagcgtcg aaaaattcc aaaaaaaat 
      ttaataatt ttttcaata agatgtcag cgaccaata 
      aaagctaca gcagttaaa ttatttgca ctttcactg 
      agaactttc ataacgatt gtccgctcc ggatggtcc 
      tattttgca gtgttgtac aacgttgga tctgaataa
    mRNA sequence
    • >TC003219 RA
      tacctttgc ggtagttcg ttttaaaac aaaacagtc 
      aaaaaatga attcattta actggcaag aaagcatta 
      aaacaaatc tgaaagctc tcagacttg tgggacttt 
      ggcccaaaa gtgattttt acaaatttg atttataca 
      cattttgca cttcactga cggtcgtag taatagttt 
      gtggtcaca atttgtcac agatcgtct acattttac 
      aagtatact ccgatttaa aagctctca cagctacaa 
      tatttgtag caagcatca attttctag gagctgtaa 
      aaatgtact ttttcatta agcacatta aaacagtga 
      agatactat tcaaaatgt taaaaacgt atcaattta 
      aaccaaaaa acattcacc aaactcagt taattaaac 
      cgtttttaa atctgtgga aaattttat acgttggtt 
      attcaataa acgtgtatc ttatcgtgg ccatgtggt 
      cgctacttc cagtcttga atggatgga cttggcaaa 
      agaaattgc cttttccag ctcgttatc cgctcgatg 
      ttacaaaat cgccgtatt atgaactag cctatgttt 
      accaattca tctgtattt ggtacataa ctgttgcca 
      atttaaatt tagatacaa ttatcatag cgttaatga 
      tgtatacat cgtgtcagt gtgaccttt tgtgcgacg 
      atttaaaaa atcttacag agacgcgat tttttgata 
      aaaaactga ttgaatgta ttaagcatc ataaagcta 
      ttttagttt ttgcagaaa aaagtaata gtttattta 
      atatgattg tcttgggtc aaattgcca caagcaccg 
      tggttctgg ccttgacaa tgttccagt tgagcatgg 
      taagccctt tgagcagtg aaggtttga atcatctct 
      tctacattg gaggtatta taatgcaaa ttctgcttt 
      actgttggt ttggaaatg aagttgaag caaagagca 
      gcaatattt tatacgcaa tttatgaat ccacttggt 
      ctgaagcgt cgaaaaatt ccaaaaaaa atttaataa 
      ttttttcaa taagatgtc agcgaccaa taaaagcta 
      cagcagtta aattatttg cactttcac tgagaactt 
      tcataacga ttgtccgct ccggatggt cctattttg 
      cagtgttgt acaacgttg gatctgaat aaactcatc 
      ttaaggtag gatattatg tactgaaac aaaacacct 
      acatgctac ttaatctta tctccgaaa actaataaa 
      tcgaaaaca acaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY