Status
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Gene information
General information
TC002657 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17148676..17156438
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC002657
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_07386

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002657 RA
        MKLNTTGKV TLECKYKDN VKRLEFVVA DVKSQPILG 
LAGCISLNL IKRIDQINK NYCTVNDVI DEYRNVFIG 
VGVLDTAHH IEIDKDIIP VVHPPRRIP LSLQPKLEE 
TLKELERQG IIEKVEYPT DWVNSLVIV EKKTGQLRL 
CLDPKDLNK AIKREHYMI PTAQDIIPR LAGKKIFTV 
IDMSAGFWH VPLDKESSD LCCFNTHVG RYKFLRMPF 
GIKSAPEVF QKHVIRIFG HISGVSVIF DDLIIVANN 
EKGHDEILQ RVLNTALEN NIKFNKDKI QYKIKSVRY 
MGNIITDKG IKPDDDKVC AIREMSEPK CKEEVKRFL 
GMINFLSSF IPNVAIINA PLRELLKQN VEWHWTHRH 
TEVFNKLKE LLSKQPTMK YFDPKENIV IQTDASKKG 
IGCCLLQNG QPVAYASRA LTETEINYA QIEKELLAV 
VYCLEKFHY YIYGRHVTV QTDHKPLLA ITRKNIGQT 
TARLQRMLL RTLKYDFHL EFIQGKHMY LADTLSRNF 
LKDRVKDDE DLESVVHSI SKHIAVTKE KKEKIKEAM 
NTDKSMLEL LNYCKNGWP KRKTQVPET IKFYWNIRD 
ELSTDDGLI FKGNRLVIP QTFREDILS QIYEGHQGI 
EKCKIRARS TVYWPGISK QIENMVMRC WICEKYRHQ 
NQKESLIPH PVPDCAWKK IAADIMTFR GMDFLIVID 
YFSNWLEII RLQGKTAAH LTIKFKEIF SRYGIPEEL 
VSDNMPFDS YAMQNRTLR EKLPIKTEC LRPQIPINI 
KRDKELAQT KTKKYYDRN ARDLPSLSK NQSVLVKTG 
RIWKPGKVV DKHEKPRSY IVQTESSFI RRNRKDLRP 
SMNAPPVFM EEQEELFKP KVNSQIQKR TEIPSSPIR 
DLTSPTRNT SPTMTTPTM PMTRPKRTI KLPQKYHDF 
VM
      CDS sequence
      • >TC002657 RA
        atgaaacta aatacgact ggaaaagtt acactagag 
tgtaaatat aaagacaat gtaaagcgt cttgagttt 
gttgttgca gatgtaaaa tctcaacct attttaggg 
ttagcaggc tgtatctca ttaaatttg attaaaaga 
attgaccaa attaacaaa aactactgt actgttaat 
gatgtaata gatgaatat agaaatgtt tttataggc 
gtaggtgta ttggacaca gcacatcac atagaaatc 
gacaaagac attattcct gttgttcat cctcctaga 
cgcattcct ttatcactc cagcccaaa ctagaagag 
accttgaaa gaattggaa cgccaagga attattgag 
aaggtggaa tatccgaca gattgggtt aacagtcta 
gtgattgtt gagaagaaa acaggacag ctgagactc 
tgcttggac cctaaggat ttgaacaaa gccataaaa 
agggagcac tatatgata cccacagcc caggatata 
attccacgt ttagctggt aagaagatt tttacagta 
atagatatg agcgctgga ttctggcat gtgccttta 
gataaggaa agttcagat ttgtgttgt ttcaacacc 
catgtgggt agatacaaa tttttacgg atgccattc 
ggaattaaa tcggctcca gaagttttt caaaaacat 
gtcattcgt atttttggc catatatcg ggagtaagc 
gttattttt gatgatctc atcatcgtg gcaaataat 
gaaaagggc cacgatgag attttacaa agagtctta 
aacacagct ctagaaaac aatataaaa tttaacaag 
gataaaatt cagtataaa ataaaaagc gtacgatat 
atgggaaac ataataacg gacaagggt ataaagcca 
gatgacgat aaagtatgt gcaatacga gaaatgtct 
gagcccaag tgcaaggaa gaagtaaaa cgttttttg 
ggaatgata aattttctt tcgtcattt attcctaat 
gtagcaata attaatgcc ccattaaga gagctactg 
aagcaaaac gttgagtgg cattggacc cataggcat 
acagaagtc ttcaataaa ttaaaagaa ttacttagt 
aaacaaccg accatgaaa tatttcgac ccaaaggaa 
aatatcgtt atccaaaca gatgcttca aagaaaggg 
ataggttgt tgtttgtta cagaacgga cagccagtg 
gcttatgct tcaagagca ttaacggaa acagaaatc 
aattacgca caaattgag aaggaactg cttgcagta 
gtgtactgt ttagaaaaa tttcattat tatatttac 
ggaagacac gtgacggtg caaacagat cacaaacca 
ctactagca ataactaga aagaatata ggacaaacg 
acagcaaga ctacaacgt atgttgtta agaacattg 
aaatatgac tttcatttg gaattcatt caaggaaaa 
catatgtat ctagctgat actctttct agaaacttt 
ttgaaggat agggtaaaa gatgatgaa gatttggaa 
tctgtagta cacagtata tcgaaacat atagctgtg 
acgaaggag aagaaagaa aaaatcaaa gaagcaatg 
aatacggac aagtcaatg ctagaactt ctgaactat 
tgcaaaaat ggttggcct aaaaggaag acacaagtg 
ccggaaaca ataaaattt tattggaat attagagac 
gaactttct accgatgac ggtttgatt ttcaaagga 
aacagatta gtcattccc cagactttt cgtgaagac 
attctatca caaatatac gaaggacat cagggcatt 
gaaaaatgt aaaattcgg gcaagatcg actgtgtac 
tggcctgga atttccaaa caaatagaa aatatggta 
atgcgttgt tggatctgt gaaaaatat agacatcaa 
aatcagaag gaatcacta attcctcac ccggttcca 
gattgcgct tggaagaag attgctgct gatataatg 
acgtttaga ggaatggat tttctaata gtaatcgac 
tatttctca aactggttg gaaataatt cgcttgcaa 
ggaaaaaca gcggcacat ttgaccatc aaatttaaa 
gaaattttt tcccgatat ggaattcct gaagagctt 
gtatcagac aacatgcct ttcgatagt tacgccatg 
caaaacagg accctcaga gaaaaacta ccgattaag 
accgagtgt ttacgacct caaattcct ataaatatt 
aaaagggat aaggagctt gcacaaacg aaaacaaaa 
aaatattat gacagaaat gcaagagat ttacctagt 
ctaagtaaa aatcaaagt gttctagtc aagacaggc 
agaatttgg aaacctgga aaagtagta gacaaacat 
gaaaaaccg aggtcatac atcgttcaa acagaatca 
agcttcatt cggcgaaat aggaaagac ctaaggcct 
tctatgaat gccccacca gtattcatg gaagaacaa 
gaagagctt ttcaaaccc aaggtaaac tctcaaatt 
caaaagcga acagaaatt cccagttcc ccaattcgt 
gacctaact agtcctacc agaaatact agcccaact 
atgacaact cctaccatg cccatgaca agaccaaaa 
cgcacaatt aaactacca cagaaatat catgatttt 
gttatgtaa
      mRNA sequence
      • >TC002657 RA
        atctggatt gttatgaaa tttagaatg cacacattt 
taggaggga actttgtta acatttggc aatatgaaa 
ctaaatacg actggaaaa gttacacta gagtgtaaa 
tataaagac aatgtaaag cgtcttgag tttgttgtt 
gcagatgta aaatctcaa cctatttta gggttagca 
ggctgtatc tcattaaat ttgattaaa agaattgac 
caaattaac aaaaactac tgtactgtt aatgatgta 
atagatgaa tatagaaat gtttttata ggcgtaggt 
gtattggac acagcacat cacatagaa atcgacaaa 
gacattatt cctgttgtt catcctcct agacgcatt 
cctttatca ctccagccc aaactagaa gagaccttg 
aaagaattg gaacgccaa ggaattatt gagaaggtg 
gaatatccg acagattgg gttaacagt ctagtgatt 
gttgagaag aaaacagga cagctgaga ctctgcttg 
gaccctaag gatttgaac aaagccata aaaagggag 
cactatatg atacccaca gcccaggat ataattcca 
cgtttagct ggtaagaag atttttaca gtaatagat 
atgagcgct ggattctgg catgtgcct ttagataag 
gaaagttca gatttgtgt tgtttcaac acccatgtg 
ggtagatac aaattttta cggatgcca ttcggaatt 
aaatcggct ccagaagtt tttcaaaaa catgtcatt 
cgtattttt ggccatata tcgggagta agcgttatt 
tttgatgat ctcatcatc gtggcaaat aatgaaaag 
ggccacgat gagatttta caaagagtc ttaaacaca 
gctctagaa aacaatata aaatttaac aaggataaa 
attcagtat aaaataaaa agcgtacga tatatggga 
aacataata acggacaag ggtataaag ccagatgac 
gataaagta tgtgcaata cgagaaatg tctgagccc 
aagtgcaag gaagaagta aaacgtttt ttgggaatg 
ataaatttt ctttcgtca tttattcct aatgtagca 
ataattaat gccccatta agagagcta ctgaagcaa 
aacgttgag tggcattgg acccatagg catacagaa 
gtcttcaat aaattaaaa gaattactt agtaaacaa 
ccgaccatg aaatatttc gacccaaag gaaaatatc 
gttatccaa acagatgct tcaaagaaa gggataggt 
tgttgtttg ttacagaac ggacagcca gtggcttat 
gcttcaaga gcattaacg gaaacagaa atcaattac 
gcacaaatt gagaaggaa ctgcttgca gtagtgtac 
tgtttagaa aaatttcat tattatatt tacggaaga 
cacgtgacg gtgcaaaca gatcacaaa ccactacta 
gcaataact agaaagaat ataggacaa acgacagca 
agactacaa cgtatgttg ttaagaaca ttgaaatat 
gactttcat ttggaattc attcaagga aaacatatg 
tatctagct gatactctt tctagaaac tttttgaag 
gatagggta aaagatgat gaagatttg gaatctgta 
gtacacagt atatcgaaa catatagct gtgacgaag 
gagaagaaa gaaaaaatc aaagaagca atgaatacg 
gacaagtca atgctagaa cttctgaac tattgcaaa 
aatggttgg cctaaaagg aagacacaa gtgccggaa 
acaataaaa ttttattgg aatattaga gacgaactt 
tctaccgat gacggtttg attttcaaa ggaaacaga 
ttagtcatt ccccagact tttcgtgaa gacattcta 
tcacaaata tacgaagga catcagggc attgaaaaa 
tgtaaaatt cgggcaaga tcgactgtg tactggcct 
ggaatttcc aaacaaata gaaaatatg gtaatgcgt 
tgttggatc tgtgaaaaa tatagacat caaaatcag 
aaggaatca ctaattcct cacccggtt ccagattgc 
gcttggaag aagattgct gctgatata atgacgttt 
agaggaatg gattttcta atagtaatc gactatttc 
tcaaactgg ttggaaata attcgcttg caaggaaaa 
acagcggca catttgacc atcaaattt aaagaaatt 
ttttcccga tatggaatt cctgaagag cttgtatca 
gacaacatg cctttcgat agttacgcc atgcaaaac 
aggaccctc agagaaaaa ctaccgatt aagaccgag 
tgtttacga cctcaaatt cctataaat attaaaagg 
gataaggag cttgcacaa acgaaaaca aaaaaatat 
tatgacaga aatgcaaga gatttacct agtctaagt 
aaaaatcaa agtgttcta gtcaagaca ggcagaatt 
tggaaacct ggaaaagta gtagacaaa catgaaaaa 
ccgaggtca tacatcgtt caaacagaa tcaagcttc 
attcggcga aataggaaa gacctaagg ccttctatg 
aatgcccca ccagtattc atggaagaa caagaagag 
cttttcaaa cccaaggta aactctcaa attcaaaag 
cgaacagaa attcccagt tccccaatt cgtgaccta 
actagtcct accagaaat actagccca actatgaca 
actcctacc atgcccatg acaagacca aaacgcaca 
attaaacta ccacagaaa tatcatgat tttgttatg 
taacgtttt cgaatttta taataattt ggttagttt 
gttttcttt ttaaacgta taaaaaaaa gggggggag 
atgtagtat atgtatctc gatgttcac atacaagta 
tggagagta gccgaatca ctcgatgtt gagggcacg 
cgctacaag aggaatgta acgaggtgt gcaataaaa 
acatgtttc tcctgtgat agtttcatt atcaaagga 
taaacccaa aataaagaa cacaacaca aa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY