General information | |||
TC002657 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 17148676..17156438 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC002657 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07386 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002657 RA
MKLNTTGKV TLECKYKDN VKRLEFVVA DVKSQPILG LAGCISLNL IKRIDQINK NYCTVNDVI DEYRNVFIG VGVLDTAHH IEIDKDIIP VVHPPRRIP LSLQPKLEE TLKELERQG IIEKVEYPT DWVNSLVIV EKKTGQLRL CLDPKDLNK AIKREHYMI PTAQDIIPR LAGKKIFTV IDMSAGFWH VPLDKESSD LCCFNTHVG RYKFLRMPF GIKSAPEVF QKHVIRIFG HISGVSVIF DDLIIVANN EKGHDEILQ RVLNTALEN NIKFNKDKI QYKIKSVRY MGNIITDKG IKPDDDKVC AIREMSEPK CKEEVKRFL GMINFLSSF IPNVAIINA PLRELLKQN VEWHWTHRH TEVFNKLKE LLSKQPTMK YFDPKENIV IQTDASKKG IGCCLLQNG QPVAYASRA LTETEINYA QIEKELLAV VYCLEKFHY YIYGRHVTV QTDHKPLLA ITRKNIGQT TARLQRMLL RTLKYDFHL EFIQGKHMY LADTLSRNF LKDRVKDDE DLESVVHSI SKHIAVTKE KKEKIKEAM NTDKSMLEL LNYCKNGWP KRKTQVPET IKFYWNIRD ELSTDDGLI FKGNRLVIP QTFREDILS QIYEGHQGI EKCKIRARS TVYWPGISK QIENMVMRC WICEKYRHQ NQKESLIPH PVPDCAWKK IAADIMTFR GMDFLIVID YFSNWLEII RLQGKTAAH LTIKFKEIF SRYGIPEEL VSDNMPFDS YAMQNRTLR EKLPIKTEC LRPQIPINI KRDKELAQT KTKKYYDRN ARDLPSLSK NQSVLVKTG RIWKPGKVV DKHEKPRSY IVQTESSFI RRNRKDLRP SMNAPPVFM EEQEELFKP KVNSQIQKR TEIPSSPIR DLTSPTRNT SPTMTTPTM PMTRPKRTI KLPQKYHDF VM
- >TC002657 RA
atgaaacta aatacgact ggaaaagtt acactagag tgtaaatat aaagacaat gtaaagcgt cttgagttt gttgttgca gatgtaaaa tctcaacct attttaggg ttagcaggc tgtatctca ttaaatttg attaaaaga attgaccaa attaacaaa aactactgt actgttaat gatgtaata gatgaatat agaaatgtt tttataggc gtaggtgta ttggacaca gcacatcac atagaaatc gacaaagac attattcct gttgttcat cctcctaga cgcattcct ttatcactc cagcccaaa ctagaagag accttgaaa gaattggaa cgccaagga attattgag aaggtggaa tatccgaca gattgggtt aacagtcta gtgattgtt gagaagaaa acaggacag ctgagactc tgcttggac cctaaggat ttgaacaaa gccataaaa agggagcac tatatgata cccacagcc caggatata attccacgt ttagctggt aagaagatt tttacagta atagatatg agcgctgga ttctggcat gtgccttta gataaggaa agttcagat ttgtgttgt ttcaacacc catgtgggt agatacaaa tttttacgg atgccattc ggaattaaa tcggctcca gaagttttt caaaaacat gtcattcgt atttttggc catatatcg ggagtaagc gttattttt gatgatctc atcatcgtg gcaaataat gaaaagggc cacgatgag attttacaa agagtctta aacacagct ctagaaaac aatataaaa tttaacaag gataaaatt cagtataaa ataaaaagc gtacgatat atgggaaac ataataacg gacaagggt ataaagcca gatgacgat aaagtatgt gcaatacga gaaatgtct gagcccaag tgcaaggaa gaagtaaaa cgttttttg ggaatgata aattttctt tcgtcattt attcctaat gtagcaata attaatgcc ccattaaga gagctactg aagcaaaac gttgagtgg cattggacc cataggcat acagaagtc ttcaataaa ttaaaagaa ttacttagt aaacaaccg accatgaaa tatttcgac ccaaaggaa aatatcgtt atccaaaca gatgcttca aagaaaggg ataggttgt tgtttgtta cagaacgga cagccagtg gcttatgct tcaagagca ttaacggaa acagaaatc aattacgca caaattgag aaggaactg cttgcagta gtgtactgt ttagaaaaa tttcattat tatatttac ggaagacac gtgacggtg caaacagat cacaaacca ctactagca ataactaga aagaatata ggacaaacg acagcaaga ctacaacgt atgttgtta agaacattg aaatatgac tttcatttg gaattcatt caaggaaaa catatgtat ctagctgat actctttct agaaacttt ttgaaggat agggtaaaa gatgatgaa gatttggaa tctgtagta cacagtata tcgaaacat atagctgtg acgaaggag aagaaagaa aaaatcaaa gaagcaatg aatacggac aagtcaatg ctagaactt ctgaactat tgcaaaaat ggttggcct aaaaggaag acacaagtg ccggaaaca ataaaattt tattggaat attagagac gaactttct accgatgac ggtttgatt ttcaaagga aacagatta gtcattccc cagactttt cgtgaagac attctatca caaatatac gaaggacat cagggcatt gaaaaatgt aaaattcgg gcaagatcg actgtgtac tggcctgga atttccaaa caaatagaa aatatggta atgcgttgt tggatctgt gaaaaatat agacatcaa aatcagaag gaatcacta attcctcac ccggttcca gattgcgct tggaagaag attgctgct gatataatg acgtttaga ggaatggat tttctaata gtaatcgac tatttctca aactggttg gaaataatt cgcttgcaa ggaaaaaca gcggcacat ttgaccatc aaatttaaa gaaattttt tcccgatat ggaattcct gaagagctt gtatcagac aacatgcct ttcgatagt tacgccatg caaaacagg accctcaga gaaaaacta ccgattaag accgagtgt ttacgacct caaattcct ataaatatt aaaagggat aaggagctt gcacaaacg aaaacaaaa aaatattat gacagaaat gcaagagat ttacctagt ctaagtaaa aatcaaagt gttctagtc aagacaggc agaatttgg aaacctgga aaagtagta gacaaacat gaaaaaccg aggtcatac atcgttcaa acagaatca agcttcatt cggcgaaat aggaaagac ctaaggcct tctatgaat gccccacca gtattcatg gaagaacaa gaagagctt ttcaaaccc aaggtaaac tctcaaatt caaaagcga acagaaatt cccagttcc ccaattcgt gacctaact agtcctacc agaaatact agcccaact atgacaact cctaccatg cccatgaca agaccaaaa cgcacaatt aaactacca cagaaatat catgatttt gttatgtaa
- >TC002657 RA
atctggatt gttatgaaa tttagaatg cacacattt taggaggga actttgtta acatttggc aatatgaaa ctaaatacg actggaaaa gttacacta gagtgtaaa tataaagac aatgtaaag cgtcttgag tttgttgtt gcagatgta aaatctcaa cctatttta gggttagca ggctgtatc tcattaaat ttgattaaa agaattgac caaattaac aaaaactac tgtactgtt aatgatgta atagatgaa tatagaaat gtttttata ggcgtaggt gtattggac acagcacat cacatagaa atcgacaaa gacattatt cctgttgtt catcctcct agacgcatt cctttatca ctccagccc aaactagaa gagaccttg aaagaattg gaacgccaa ggaattatt gagaaggtg gaatatccg acagattgg gttaacagt ctagtgatt gttgagaag aaaacagga cagctgaga ctctgcttg gaccctaag gatttgaac aaagccata aaaagggag cactatatg atacccaca gcccaggat ataattcca cgtttagct ggtaagaag atttttaca gtaatagat atgagcgct ggattctgg catgtgcct ttagataag gaaagttca gatttgtgt tgtttcaac acccatgtg ggtagatac aaattttta cggatgcca ttcggaatt aaatcggct ccagaagtt tttcaaaaa catgtcatt cgtattttt ggccatata tcgggagta agcgttatt tttgatgat ctcatcatc gtggcaaat aatgaaaag ggccacgat gagatttta caaagagtc ttaaacaca gctctagaa aacaatata aaatttaac aaggataaa attcagtat aaaataaaa agcgtacga tatatggga aacataata acggacaag ggtataaag ccagatgac gataaagta tgtgcaata cgagaaatg tctgagccc aagtgcaag gaagaagta aaacgtttt ttgggaatg ataaatttt ctttcgtca tttattcct aatgtagca ataattaat gccccatta agagagcta ctgaagcaa aacgttgag tggcattgg acccatagg catacagaa gtcttcaat aaattaaaa gaattactt agtaaacaa ccgaccatg aaatatttc gacccaaag gaaaatatc gttatccaa acagatgct tcaaagaaa gggataggt tgttgtttg ttacagaac ggacagcca gtggcttat gcttcaaga gcattaacg gaaacagaa atcaattac gcacaaatt gagaaggaa ctgcttgca gtagtgtac tgtttagaa aaatttcat tattatatt tacggaaga cacgtgacg gtgcaaaca gatcacaaa ccactacta gcaataact agaaagaat ataggacaa acgacagca agactacaa cgtatgttg ttaagaaca ttgaaatat gactttcat ttggaattc attcaagga aaacatatg tatctagct gatactctt tctagaaac tttttgaag gatagggta aaagatgat gaagatttg gaatctgta gtacacagt atatcgaaa catatagct gtgacgaag gagaagaaa gaaaaaatc aaagaagca atgaatacg gacaagtca atgctagaa cttctgaac tattgcaaa aatggttgg cctaaaagg aagacacaa gtgccggaa acaataaaa ttttattgg aatattaga gacgaactt tctaccgat gacggtttg attttcaaa ggaaacaga ttagtcatt ccccagact tttcgtgaa gacattcta tcacaaata tacgaagga catcagggc attgaaaaa tgtaaaatt cgggcaaga tcgactgtg tactggcct ggaatttcc aaacaaata gaaaatatg gtaatgcgt tgttggatc tgtgaaaaa tatagacat caaaatcag aaggaatca ctaattcct cacccggtt ccagattgc gcttggaag aagattgct gctgatata atgacgttt agaggaatg gattttcta atagtaatc gactatttc tcaaactgg ttggaaata attcgcttg caaggaaaa acagcggca catttgacc atcaaattt aaagaaatt ttttcccga tatggaatt cctgaagag cttgtatca gacaacatg cctttcgat agttacgcc atgcaaaac aggaccctc agagaaaaa ctaccgatt aagaccgag tgtttacga cctcaaatt cctataaat attaaaagg gataaggag cttgcacaa acgaaaaca aaaaaatat tatgacaga aatgcaaga gatttacct agtctaagt aaaaatcaa agtgttcta gtcaagaca ggcagaatt tggaaacct ggaaaagta gtagacaaa catgaaaaa ccgaggtca tacatcgtt caaacagaa tcaagcttc attcggcga aataggaaa gacctaagg ccttctatg aatgcccca ccagtattc atggaagaa caagaagag cttttcaaa cccaaggta aactctcaa attcaaaag cgaacagaa attcccagt tccccaatt cgtgaccta actagtcct accagaaat actagccca actatgaca actcctacc atgcccatg acaagacca aaacgcaca attaaacta ccacagaaa tatcatgat tttgttatg taacgtttt cgaatttta taataattt ggttagttt gttttcttt ttaaacgta taaaaaaaa gggggggag atgtagtat atgtatctc gatgttcac atacaagta tggagagta gccgaatca ctcgatgtt gagggcacg cgctacaag aggaatgta acgaggtgt gcaataaaa acatgtttc tcctgtgat agtttcatt atcaaagga taaacccaa aataaagaa cacaacaca aa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
