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Gene information
General information
TC002657 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17148676..17156438
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002657
      iBeetle screen iB_07386

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002657 RA
        MKLNTTGKV TLECKYKDN VKRLEFVVA DVKSQPILG 
        LAGCISLNL IKRIDQINK NYCTVNDVI DEYRNVFIG 
        VGVLDTAHH IEIDKDIIP VVHPPRRIP LSLQPKLEE 
        TLKELERQG IIEKVEYPT DWVNSLVIV EKKTGQLRL 
        CLDPKDLNK AIKREHYMI PTAQDIIPR LAGKKIFTV 
        IDMSAGFWH VPLDKESSD LCCFNTHVG RYKFLRMPF 
        GIKSAPEVF QKHVIRIFG HISGVSVIF DDLIIVANN 
        EKGHDEILQ RVLNTALEN NIKFNKDKI QYKIKSVRY 
        MGNIITDKG IKPDDDKVC AIREMSEPK CKEEVKRFL 
        GMINFLSSF IPNVAIINA PLRELLKQN VEWHWTHRH 
        TEVFNKLKE LLSKQPTMK YFDPKENIV IQTDASKKG 
        IGCCLLQNG QPVAYASRA LTETEINYA QIEKELLAV 
        VYCLEKFHY YIYGRHVTV QTDHKPLLA ITRKNIGQT 
        TARLQRMLL RTLKYDFHL EFIQGKHMY LADTLSRNF 
        LKDRVKDDE DLESVVHSI SKHIAVTKE KKEKIKEAM 
        NTDKSMLEL LNYCKNGWP KRKTQVPET IKFYWNIRD 
        ELSTDDGLI FKGNRLVIP QTFREDILS QIYEGHQGI 
        EKCKIRARS TVYWPGISK QIENMVMRC WICEKYRHQ 
        NQKESLIPH PVPDCAWKK IAADIMTFR GMDFLIVID 
        YFSNWLEII RLQGKTAAH LTIKFKEIF SRYGIPEEL 
        VSDNMPFDS YAMQNRTLR EKLPIKTEC LRPQIPINI 
        KRDKELAQT KTKKYYDRN ARDLPSLSK NQSVLVKTG 
        RIWKPGKVV DKHEKPRSY IVQTESSFI RRNRKDLRP 
        SMNAPPVFM EEQEELFKP KVNSQIQKR TEIPSSPIR 
        DLTSPTRNT SPTMTTPTM PMTRPKRTI KLPQKYHDF 
        VM
      CDS sequence
      • >TC002657 RA
        atgaaacta aatacgact ggaaaagtt acactagag 
        tgtaaatat aaagacaat gtaaagcgt cttgagttt 
        gttgttgca gatgtaaaa tctcaacct attttaggg 
        ttagcaggc tgtatctca ttaaatttg attaaaaga 
        attgaccaa attaacaaa aactactgt actgttaat 
        gatgtaata gatgaatat agaaatgtt tttataggc 
        gtaggtgta ttggacaca gcacatcac atagaaatc 
        gacaaagac attattcct gttgttcat cctcctaga 
        cgcattcct ttatcactc cagcccaaa ctagaagag 
        accttgaaa gaattggaa cgccaagga attattgag 
        aaggtggaa tatccgaca gattgggtt aacagtcta 
        gtgattgtt gagaagaaa acaggacag ctgagactc 
        tgcttggac cctaaggat ttgaacaaa gccataaaa 
        agggagcac tatatgata cccacagcc caggatata 
        attccacgt ttagctggt aagaagatt tttacagta 
        atagatatg agcgctgga ttctggcat gtgccttta 
        gataaggaa agttcagat ttgtgttgt ttcaacacc 
        catgtgggt agatacaaa tttttacgg atgccattc 
        ggaattaaa tcggctcca gaagttttt caaaaacat 
        gtcattcgt atttttggc catatatcg ggagtaagc 
        gttattttt gatgatctc atcatcgtg gcaaataat 
        gaaaagggc cacgatgag attttacaa agagtctta 
        aacacagct ctagaaaac aatataaaa tttaacaag 
        gataaaatt cagtataaa ataaaaagc gtacgatat 
        atgggaaac ataataacg gacaagggt ataaagcca 
        gatgacgat aaagtatgt gcaatacga gaaatgtct 
        gagcccaag tgcaaggaa gaagtaaaa cgttttttg 
        ggaatgata aattttctt tcgtcattt attcctaat 
        gtagcaata attaatgcc ccattaaga gagctactg 
        aagcaaaac gttgagtgg cattggacc cataggcat 
        acagaagtc ttcaataaa ttaaaagaa ttacttagt 
        aaacaaccg accatgaaa tatttcgac ccaaaggaa 
        aatatcgtt atccaaaca gatgcttca aagaaaggg 
        ataggttgt tgtttgtta cagaacgga cagccagtg 
        gcttatgct tcaagagca ttaacggaa acagaaatc 
        aattacgca caaattgag aaggaactg cttgcagta 
        gtgtactgt ttagaaaaa tttcattat tatatttac 
        ggaagacac gtgacggtg caaacagat cacaaacca 
        ctactagca ataactaga aagaatata ggacaaacg 
        acagcaaga ctacaacgt atgttgtta agaacattg 
        aaatatgac tttcatttg gaattcatt caaggaaaa 
        catatgtat ctagctgat actctttct agaaacttt 
        ttgaaggat agggtaaaa gatgatgaa gatttggaa 
        tctgtagta cacagtata tcgaaacat atagctgtg 
        acgaaggag aagaaagaa aaaatcaaa gaagcaatg 
        aatacggac aagtcaatg ctagaactt ctgaactat 
        tgcaaaaat ggttggcct aaaaggaag acacaagtg 
        ccggaaaca ataaaattt tattggaat attagagac 
        gaactttct accgatgac ggtttgatt ttcaaagga 
        aacagatta gtcattccc cagactttt cgtgaagac 
        attctatca caaatatac gaaggacat cagggcatt 
        gaaaaatgt aaaattcgg gcaagatcg actgtgtac 
        tggcctgga atttccaaa caaatagaa aatatggta 
        atgcgttgt tggatctgt gaaaaatat agacatcaa 
        aatcagaag gaatcacta attcctcac ccggttcca 
        gattgcgct tggaagaag attgctgct gatataatg 
        acgtttaga ggaatggat tttctaata gtaatcgac 
        tatttctca aactggttg gaaataatt cgcttgcaa 
        ggaaaaaca gcggcacat ttgaccatc aaatttaaa 
        gaaattttt tcccgatat ggaattcct gaagagctt 
        gtatcagac aacatgcct ttcgatagt tacgccatg 
        caaaacagg accctcaga gaaaaacta ccgattaag 
        accgagtgt ttacgacct caaattcct ataaatatt 
        aaaagggat aaggagctt gcacaaacg aaaacaaaa 
        aaatattat gacagaaat gcaagagat ttacctagt 
        ctaagtaaa aatcaaagt gttctagtc aagacaggc 
        agaatttgg aaacctgga aaagtagta gacaaacat 
        gaaaaaccg aggtcatac atcgttcaa acagaatca 
        agcttcatt cggcgaaat aggaaagac ctaaggcct 
        tctatgaat gccccacca gtattcatg gaagaacaa 
        gaagagctt ttcaaaccc aaggtaaac tctcaaatt 
        caaaagcga acagaaatt cccagttcc ccaattcgt 
        gacctaact agtcctacc agaaatact agcccaact 
        atgacaact cctaccatg cccatgaca agaccaaaa 
        cgcacaatt aaactacca cagaaatat catgatttt 
        gttatgtaa
      mRNA sequence
      • >TC002657 RA
        atctggatt gttatgaaa tttagaatg cacacattt 
        taggaggga actttgtta acatttggc aatatgaaa 
        ctaaatacg actggaaaa gttacacta gagtgtaaa 
        tataaagac aatgtaaag cgtcttgag tttgttgtt 
        gcagatgta aaatctcaa cctatttta gggttagca 
        ggctgtatc tcattaaat ttgattaaa agaattgac 
        caaattaac aaaaactac tgtactgtt aatgatgta 
        atagatgaa tatagaaat gtttttata ggcgtaggt 
        gtattggac acagcacat cacatagaa atcgacaaa 
        gacattatt cctgttgtt catcctcct agacgcatt 
        cctttatca ctccagccc aaactagaa gagaccttg 
        aaagaattg gaacgccaa ggaattatt gagaaggtg 
        gaatatccg acagattgg gttaacagt ctagtgatt 
        gttgagaag aaaacagga cagctgaga ctctgcttg 
        gaccctaag gatttgaac aaagccata aaaagggag 
        cactatatg atacccaca gcccaggat ataattcca 
        cgtttagct ggtaagaag atttttaca gtaatagat 
        atgagcgct ggattctgg catgtgcct ttagataag 
        gaaagttca gatttgtgt tgtttcaac acccatgtg 
        ggtagatac aaattttta cggatgcca ttcggaatt 
        aaatcggct ccagaagtt tttcaaaaa catgtcatt 
        cgtattttt ggccatata tcgggagta agcgttatt 
        tttgatgat ctcatcatc gtggcaaat aatgaaaag 
        ggccacgat gagatttta caaagagtc ttaaacaca 
        gctctagaa aacaatata aaatttaac aaggataaa 
        attcagtat aaaataaaa agcgtacga tatatggga 
        aacataata acggacaag ggtataaag ccagatgac 
        gataaagta tgtgcaata cgagaaatg tctgagccc 
        aagtgcaag gaagaagta aaacgtttt ttgggaatg 
        ataaatttt ctttcgtca tttattcct aatgtagca 
        ataattaat gccccatta agagagcta ctgaagcaa 
        aacgttgag tggcattgg acccatagg catacagaa 
        gtcttcaat aaattaaaa gaattactt agtaaacaa 
        ccgaccatg aaatatttc gacccaaag gaaaatatc 
        gttatccaa acagatgct tcaaagaaa gggataggt 
        tgttgtttg ttacagaac ggacagcca gtggcttat 
        gcttcaaga gcattaacg gaaacagaa atcaattac 
        gcacaaatt gagaaggaa ctgcttgca gtagtgtac 
        tgtttagaa aaatttcat tattatatt tacggaaga 
        cacgtgacg gtgcaaaca gatcacaaa ccactacta 
        gcaataact agaaagaat ataggacaa acgacagca 
        agactacaa cgtatgttg ttaagaaca ttgaaatat 
        gactttcat ttggaattc attcaagga aaacatatg 
        tatctagct gatactctt tctagaaac tttttgaag 
        gatagggta aaagatgat gaagatttg gaatctgta 
        gtacacagt atatcgaaa catatagct gtgacgaag 
        gagaagaaa gaaaaaatc aaagaagca atgaatacg 
        gacaagtca atgctagaa cttctgaac tattgcaaa 
        aatggttgg cctaaaagg aagacacaa gtgccggaa 
        acaataaaa ttttattgg aatattaga gacgaactt 
        tctaccgat gacggtttg attttcaaa ggaaacaga 
        ttagtcatt ccccagact tttcgtgaa gacattcta 
        tcacaaata tacgaagga catcagggc attgaaaaa 
        tgtaaaatt cgggcaaga tcgactgtg tactggcct 
        ggaatttcc aaacaaata gaaaatatg gtaatgcgt 
        tgttggatc tgtgaaaaa tatagacat caaaatcag 
        aaggaatca ctaattcct cacccggtt ccagattgc 
        gcttggaag aagattgct gctgatata atgacgttt 
        agaggaatg gattttcta atagtaatc gactatttc 
        tcaaactgg ttggaaata attcgcttg caaggaaaa 
        acagcggca catttgacc atcaaattt aaagaaatt 
        ttttcccga tatggaatt cctgaagag cttgtatca 
        gacaacatg cctttcgat agttacgcc atgcaaaac 
        aggaccctc agagaaaaa ctaccgatt aagaccgag 
        tgtttacga cctcaaatt cctataaat attaaaagg 
        gataaggag cttgcacaa acgaaaaca aaaaaatat 
        tatgacaga aatgcaaga gatttacct agtctaagt 
        aaaaatcaa agtgttcta gtcaagaca ggcagaatt 
        tggaaacct ggaaaagta gtagacaaa catgaaaaa 
        ccgaggtca tacatcgtt caaacagaa tcaagcttc 
        attcggcga aataggaaa gacctaagg ccttctatg 
        aatgcccca ccagtattc atggaagaa caagaagag 
        cttttcaaa cccaaggta aactctcaa attcaaaag 
        cgaacagaa attcccagt tccccaatt cgtgaccta 
        actagtcct accagaaat actagccca actatgaca 
        actcctacc atgcccatg acaagacca aaacgcaca 
        attaaacta ccacagaaa tatcatgat tttgttatg 
        taacgtttt cgaatttta taataattt ggttagttt 
        gttttcttt ttaaacgta taaaaaaaa gggggggag 
        atgtagtat atgtatctc gatgttcac atacaagta 
        tggagagta gccgaatca ctcgatgtt gagggcacg 
        cgctacaag aggaatgta acgaggtgt gcaataaaa 
        acatgtttc tcctgtgat agtttcatt atcaaagga 
        taaacccaa aataaagaa cacaacaca aa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY