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Gene information
General information
TC001380 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15228471..15229312
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001380
    iBeetle screen iB_07381

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001380 RA
      MAPYKIVMV RHGESEWNQ KNLFCGWYD AKLSEKGKQ 
      EAANAGKTL KDAGYKFDQ AYTSVLSRA QDTLQIILK 
      TLNQCDIPI QTTWRLNER HYGALTGLN KTETAAKYG 
      EQQVAIWRR SFDVPPPPM ENDHPHYKD IVCDPRYKN 
      GPPPEQFPK FESLKLTIE RTLPFWNET IVPQIKAGK 
      RILIAAHGN SLRGIVKHL DQLNDEQIM GLNLPTGIP 
      FVYMLQENM KPAQGGSLQ FLGDPETVK KAMEAVANQ 
      GKAK
    CDS sequence
    • >TC001380 RA
      atggcaccg tataaaatc gtaatggtc cgtcacggc 
      gaatcagaa tggaaccaa aaaaacctt ttttgtggt 
      tggtacgac gcaaaattg agcgaaaaa ggtaaacaa 
      gaagcggca aacgccggc aaaaccctc aaagacgcc 
      ggctataaa ttcgaccaa gcttacact tcggtccta 
      agtcgagca caagacact cttcagatt atcttaaaa 
      acgctaaat caatgcgat attcccatt caaacgaca 
      tggcgtctc aatgaacgt cattacggc gcattaacc 
      ggccttaac aaaactgaa actgccgcc aaatacggc 
      gaacaacaa gtggccatt tggaggcgg agttttgac 
      gtaccgccg ccccccatg gaaaatgac catccacac 
      tataaagac atcgtatgc gatccgaga tacaaaaac 
      ggaccgcct cctgaacaa ttccccaaa tttgaatcg 
      ctcaaattg acaattgaa cgaactttg cccttttgg 
      aacgaaact attgtacca caaattaaa gccgggaaa 
      agaattctg attgcagcg cacggaaat agtctaaga 
      ggaatcgtc aaacattta gatcaactt aatgatgaa 
      caaataatg ggacttaat ctaccgact ggaattcca 
      ttcgtttat atgctgcaa gagaacatg aaaccagca 
      caaggaggc agtttgcaa tttttaggc gatccagaa 
      acggtcaag aaggccatg gaagccgta gccaaccaa 
      ggaaaagcc aaataa
    mRNA sequence
    • >TC001380 RA
      aattacagt acaatggca ccgtataaa atcgtaatg 
      gtccgtcac ggcgaatca gaatggaac caaaaaaac 
      cttttttgt ggttggtac gacgcaaaa ttgagcgaa 
      aaaggtaaa caagaagcg gcaaacgcc ggcaaaacc 
      ctcaaagac gccggctat aaattcgac caagcttac 
      acttcggtc ctaagtcga gcacaagac actcttcag 
      attatctta aaaacgcta aatcaatgc gatattccc 
      attcaaacg acatggcgt ctcaatgaa cgtcattac 
      ggcgcatta accggcctt aacaaaact gaaactgcc 
      gccaaatac ggcgaacaa caagtggcc atttggagg 
      cggagtttt gacgtaccg ccgcccccc atggaaaat 
      gaccatcca cactataaa gacatcgta tgcgatccg 
      agatacaaa aacggaccg cctcctgaa caattcccc 
      aaatttgaa tcgctcaaa ttgacaatt gaacgaact 
      ttgcccttt tggaacgaa actattgta ccacaaatt 
      aaagccggg aaaagaatt ctgattgca gcgcacgga 
      aatagtcta agaggaatc gtcaaacat ttagatcaa 
      cttaatgat gaacaaata atgggactt aatctaccg 
      actggaatt ccattcgtt tatatgctg caagagaac 
      atgaaacca gcacaagga ggcagtttg caattttta 
      ggcgatcca gaaacggtc aagaaggcc atggaagcc 
      gtagccaac caaggaaaa gccaaataa ttatataca 
      atgttattt tctataaac aacgatttg cttaataat 
      tattatcat gtttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY