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Gene information
General information
TC000766 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5731601..5732708
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000766
    iBeetle screen iB_07376

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 1
    mature egg not present - Number of animals showing the phenotype: 3

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    labium potentially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs partially potentiallyfused - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000766 RA
      MLKILTLPC FLFIIRSSL ASNLEHDGW 
      ANIKLEHCL VPSASPVFT ERGNITIQS 
      LRLGQAIVK QNPLTEQEK NQLRDLAAK 
      NQFYQIRST VVASDGAEN TFLSTIKAC 
      MLAESELDD KLSVSLDYT GRVIGVTLL 
      IASSSTCEG AFVPLSKLK QFTTHVYVR 
      HSDVGPIPN TQSYIEKLE REKEARERG 
      EVKDNRSIL AKYWMYIVP VVILLMVSS 
      MANPEAAAG NGGGAGR
    CDS sequence
    • >TC000766 RA
      atgttaaaa attctcaca ttgccgtgt tttttattt 
      attatcaga tctagtctt gctagcaat ttggagcac 
      gatggttgg gccaatatt aaattagag cattgtcta 
      gtcccttca gcaagccct gtttttacc gaaagggga 
      aacatcact attcaaagt ttgaggtta gggcaagca 
      atcgtcaaa cagaacccc ttgactgaa caagaaaaa 
      aatcagtta agagatttg gctgctaaa aatcagttt 
      taccaaatc aggtcaaca gtggtcgca agcgatggt 
      gcagaaaac acattttta tccaccatt aaagcttgt 
      atgctggct gagtctgaa ctggatgat aagctgtcg 
      gtgtcttta gattatacg ggaagggta attggtgtc 
      actcttctt attgcatca agttcaaca tgtgaaggg 
      gcttttgtg cctttaagt aaattgaaa cagtttacc 
      actcatgtt tatgtaaga cacagtgat gttggacct 
      atacctaat acacaatcg tacattgaa aaacttgaa 
      cgagaaaaa gaggcacgg gaaagaggc gaagtcaaa 
      gacaataga tcaatttta gcaaaatac tggatgtat 
      atcgtgcct gttgttatt ttgcttatg gtttcatca 
      atggcaaac cctgaagct gctgcaggg aacgggggc 
      ggagcaggc agataa
    mRNA sequence
    • >TC000766 RA
      caatttatt gttaataac aataacata attttgatg 
      tacaattaa ttctcgtgc agcggattt tgagtgtca 
      atcacaggt gtcaatttg acgtttcga aaattttga 
      taaatcaat tttggatta ttcgatttt gattttgag 
      tttatttgt gattgtgtt acaaagtgt taacatgtt 
      aaaaattct cacattgcc gtgtttttt atttattat 
      cagatctag tcttgctag caatttgga gcacgatgg 
      ttgggccaa tattaaatt agagcattg tctagtccc 
      ttcagcaag ccctgtttt taccgaaag gggaaacat 
      cactattca aagtttgag gttagggca agcaatcgt 
      caaacagaa ccccttgac tgaacaaga aaaaaatca 
      gttaagaga tttggctgc taaaaatca gttttacca 
      aatcaggtc aacagtggt cgcaagcga tggtgcaga 
      aaacacatt tttatccac cattaaagc ttgtatgct 
      ggctgagtc tgaactgga tgataagct gtcggtgtc 
      tttagatta tacgggaag ggtaattgg tgtcactct 
      tcttattgc atcaagttc aacatgtga aggggcttt 
      tgtgccttt aagtaaatt gaaacagtt taccactca 
      tgtttatgt aagacacag tgatgttgg acctatacc 
      taatacaca atcgtacat tgaaaaact tgaacgaga 
      aaaagaggc acgggaaag aggcgaagt caaagacaa 
      tagatcaat tttagcaaa atactggat gtatatcgt 
      gcctgttgt tattttgct tatggtttc atcaatggc 
      aaaccctga agctgctgc agggaacgg gggcggagc 
      aggcagata aaaaattgt tacgtaaat ttatttatt 
      tactaatag a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY