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Gene information
General information
TC010295 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451049.1: 175483..176414
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010295
      iBeetle screen iB_07357

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      head capsule not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      labrum size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      labium sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010295 RA
        MIMKCMEEF EKHLETARN QSKKAKRTL LSNLEEMEI 
        RNEEIQESR EHFAELRNN GVTLEKFVR FFEEWLKKS 
        RLTGDKIRL RHSSLKVQY HKAATLLAN KKLIGEHVQ 
        EVDFDQLRV ENRHLTEHI ESKNCLLIE MKKMTASGN 
        LKLTQLKKF LQEKMAKKE ALLQSIKTF EDDIMRSDE 
        QLEKLYKEL DQAKKKYDQ CKERSENYL APDVLECIR 
        KKADVDNAK KELKVWERR KMLKELVLS IAITEMKRI 
        FRCTNTHPS WFK
      CDS sequence
      • >TC010295 RA
        atgatcatg aaatgtatg gaagagttt gaaaaacac 
        ctcgaaacg gctaggaat cagtcaaag aaagcgaaa 
        cgtacgctg ctctcaaac ctcgaagag atggaaatt 
        aggaacgag gaaatccaa gaatcgcgg gaacatttc 
        gcagaattg agaaacaac ggtgtcacg cttgaaaaa 
        ttcgtccgg tttttcgag gaatggttg aagaaatct 
        cgcctaact ggggataaa atcagactg agacacagt 
        tcacttaag gtgcaatac cacaaagct gcgactctt 
        ctcgctaat aagaaacta ataggggaa cacgtgcag 
        gaggttgat tttgatcaa ctccgagtc gagaatcgg 
        catttgact gagcatatt gagagcaaa aactgtctt 
        ctaatcgaa atgaagaaa atgacagcc agtggtaat 
        ttaaaactg actcagttg aagaaattc ctacaggag 
        aaaatggcc aagaaggaa gcgctgctt caaagtatt 
        aaaacgttt gaagatgat attatgcgg tcggatgag 
        cagttagag aagctttat aaagagttg gatcaagcc 
        aagaaaaag tacgaccag tgcaaagaa cgatctgaa 
        aattatttg gcgcccgat gttttggag tgtattcga 
        aagaaggca gatgttgat aatgcgaaa aaagagtta 
        aaagtttgg gaaaggcga aagatgctg aaggaacta 
        gttctgagt attgcaata actgaaatg aagaggatt 
        ttcaggtgt acaaataca cacccgtct tggttcaaa 
        tag
      mRNA sequence
      • >TC010295 RA
        atagtagtc actttgagt ccgatcttt actctgata 
        aagaagcac cgaaaataa tcctcgccc aaaaactgg 
        acatgatca tgaaatgta tggaagagt ttgaaaaac 
        acctcgaaa cggctagga atcagtcaa agaaagcga 
        aacgtacgc tgctctcaa acctcgaag agatggaaa 
        ttaggaacg aggaaatcc aagaatcgc gggaacatt 
        tcgcagaat tgagaaaca acggtgtca cgcttgaaa 
        aattcgtcc ggtttttcg aggaatggt tgaagaaat 
        ctcgcctaa ctggggata aaatcagac tgagacaca 
        gttcactta aggtgcaat accacaaag ctgcgactc 
        ttctcgcta ataagaaac taatagggg aacacgtgc 
        aggaggttg attttgatc aactccgag tcgagaatc 
        ggcatttga ctgagcata ttgagagca aaaactgtc 
        ttctaatcg aaatgaaga aaatgacag ccagtggta 
        atttaaaac tgactcagt tgaagaaat tcctacagg 
        agaaaatgg ccaagaagg aagcgctgc ttcaaagta 
        ttaaaacgt ttgaagatg atattatgc ggtcggatg 
        agcagttag agaagcttt ataaagagt tggatcaag 
        ccaagaaaa agtacgacc agtgcaaag aacgatctg 
        aaaattatt tggcgcccg atgttttgg agtgtattc 
        gaaagaagg cagatgttg ataatgcga aaaaagagt 
        taaaagttt gggaaaggc gaaagatgc tgaaggaac 
        tagttctga gtattgcaa taactgaaa tgaagagga 
        ttttcaggt gtacaaata cacacccgt cttggttca 
        aataggctt taactgtgc attatgtta gttttttta 
        atttgtgtt ttaaataaa tgatacagt aaaaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY