Status
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Gene information
General information
TC031212
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008753,TC008752,TC008744
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9402707..9514206
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031212
      iBeetle screen iB_07344

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031212 RA
        MSAKRKPSA IIQHHKESL STPESSEAN EEDHFAGTG 
        AMKDSDKLK LMLLAWNYH NQAQGNRNG DIPDLSSVP 
        GGEEMAGLW AQYHSALGL AKGKATPPT TRDQSSPVN 
        ADTGSAHDE TSSSGNKED EDDDDDESD DKIDTQTHD 
        PERLKAFNM FVRLFVDEN LDRMVPISK QPKEKIQAI 
        IDSCTRQFP EFAERARKR IRTYLKSCR RNKRARDPN 
        SPWDATRPT PAHLTSVQA EQILATACE NESHNAKRM 
        RLGLEPVSQ PMPILPGAQ PTDTTAPIN RGIDFSNNT 
        PVKIEVDSV STAFPSSTT PSSTPNSVS KLSLTPDLK 
        TSAMSVSNA LGATAMNGI GISSTAGSA PTAMYRPNF 
        SQAFQRAGP YPLFPGANP FGTGTGLLT NGPADLSMK 
        QKPLLNHKL STGEMAAVR QLITGYRES AAFLLRSAD 
        ELEQLLIQQ Q
      • >TC031212 RB
        MSAKRKPSA IIQHHKESL STPESSEAN EEDHFAGTG 
        AMKDSDKLK LMLLAWNYH NQAQGNRNG DIPDLSSVP 
        GGEEMAGLW AQYHSALGL AKGKATPPT TRDQSSPVN 
        ADTGSAHDE TSSSGNKED EDDDDDESD DKIDTQTHD 
        PERLKAFNM FVRLFVDEN LDRMVPISK QPKEKIQAI 
        IDSCTRQFP EFAERARKR IRTYLKSCR RNKRARDPN 
        SPWDATRPT PAHLTSVQA EQILATACE NESHNAKRM 
        RLGLEPVSQ PMPILPGAQ PTDTTAPIN RGIDFSNNT 
        PVKIEVDSV STAFPSSTT PSSTPNSVS KLSLTPDLK 
        TSAMSVSNA LGATAMNGI GISSTAGSA PTAMYRPNF 
        SQAFQRAGP YPLFPGANP FGTGTGLLT NDLSMKQKP 
        LLNHKLSTG EMAAVRQLI TGYRESAAF LLRSADELE 
        QLLIQQQ
      • >TC031212 RC
        MLADRGVRR KMAQEEARP KKAPKRKPP TPAPKSRAE 
        QNNNVKRQR LSVDNKDDM LATYQPWVI QTYGDLAKT 
        KTITLKKYA RILRTLRGE EIVSADNSK FRFWVKAKG 
        FHVGKPAGY EAKPADAIV GRYSVCDAD EGIPAVNEK 
        LAGSAKDPP LYVPNAPFK NSTGEPIYR KVAVVENFF 
        DIIYNVHVE LEGRPGKHA GQKRTYRTI TETYAFLPR 
        EAVTRFLLG CTECQKHPR SPSPASVLP TPSPSPTLP 
        LVHTPAPPL PTPPPAPPA EAPYRVPPA AESLDLSRS 
        NGEVQKPES RTGTPEPKR SSNPLDVCN LTAKDPPKT 
        PPKKRHLNG SEPKQTPPK LWSPVESIE REQQESRKR 
        RVLMAGDID YSLPITTTY LKYMRSLGC TDEDALKFE 
        NKHESLSTP ESSEANEED HFAGTGAMK DSDKLKLML 
        LAWNYHNQA QGNRNGDIP DLSSVPGGE EMAGLWAQY 
        HSALGLAKG KATPPTTRD QSSPVNADT GSAHDETSS 
        SGNKEDEDD DDDESDDKI DTQTHDPER LKAFNMFVR 
        LFVDENLDR MVPISKQPK EKIQAIIDS CTRQFPEFA 
        ERARKRIRT YLKSCRRNK RARDPNSPW DATRPTPAH 
        LTSVQAEQI LATACENES HNAKRMRLG LEPVSQPMP 
        ILPGAQPTD TTAPINRGI DFSNNTPVK IEVDSVSTA 
        FPSSTTPSS TPNSVSKLS LTPDLKTSA MSVSNALGA 
        TAMNGIGIS STAGSAPTA MYRPNFSQA FQRAGPYPL 
        FPGANPFGT GTGLLTNGP ADLSMKQKP LLNHKLSTG 
        EMAAVRQLI TGYRESAAF LLRSADELE QLLIQQQ
      • >TC031212 RD
        MLADRGVRR KMAQEEARP KKAPKRKPP TPAPKSRAE 
        QNNNVKRQR LSVDNKDDM LATYQPWVI QTYGDLAKT 
        KTITLKKYA RILRTLRGE EIVSADNSK FRFWVKAKG 
        FHVGKPAGY EAKPADAIV GRYSVCDAD EGIPAVNEK 
        LAGSAKDPP LYVPNAPFK NSTGEPIYR KVAVVENFF 
        DIIYNVHVE LEGRPGKHA GQKRTYRTI TETYAFLPR 
        EAVTRFLLG CTECQKHPR SPSPASVLP TPSPSPTLP 
        LVHTPAPPL PTPPPAPPA EAPYRVPPA AESLDLSRS 
        NGEVQKPES RTGTPEPKR SSNPLDVCN LTAKDPPKT 
        PPKKRHLNG SEPKQTPPK LWSPVESIE REQQESRKR 
        RVLMAGDID YSLPITTTY LKYMRSLGC TDEDALKFE 
        NKHVSLVIE HFLHEAISH YFY
      CDS sequence
      • >TC031212 RA
        atgtcggcc aaacgtaag cccagtgcc attatccag 
        caccataag gagagtctg tccacgccg gagtcgtcc 
        gaagcgaac gaggaggac cactttgcg ggaaccggg 
        gctatgaaa gacagcgac aagctcaag ctgatgctc 
        ttggcgtgg aactaccac aatcaggcc caagggaac 
        agaaatggg gacatcccg gacctgtcc tcggttccg 
        ggaggcgag gaaatggcc ggcctgtgg gcccagtac 
        cactcggct ctggggcta gcgaaaggc aaggccacg 
        ccgcccacc actcgagat caatctagt ccagtcaat 
        gctgatacg gggtcagcc catgacgaa accagctcc 
        agcggtaac aaagaagac gaagacgat gacgatgat 
        gaaagcgat gacaagatc gacacacaa acgcacgac 
        cctgaacga ctcaaagcc ttcaatatg ttcgtccgt 
        ctgttcgtc gacgaaaac ctggaccgc atggtgccg 
        attagcaaa cagccgaaa gaaaagatc caagcaata 
        atcgattcg tgcacgagg cagtttccg gagttcgcg 
        gaacgagcc cggaagcgg atccgtact tacctcaag 
        tcttgtaga agaaacaaa cgtgccaga gacccgaac 
        tcgccatgg gacgcgacg cgaccgact ccagctcac 
        ttgacatca gtacaagca gaacaaatt ttagcaacg 
        gcctgcgag aacgaaagc cacaatgcg aaaagaatg 
        cgattgggg ctagaaccg gtcagtcag cccatgcca 
        atattaccg ggggcccag cctacggac accacagcc 
        ccgatcaac agaggaata gacttttcg aataacact 
        ccagtgaag atcgaggtg gactcggtc tcgacggcc 
        ttcccgtcg tcgacgacg ccctcctcc acccccaac 
        tcggtctcc aagctgtct ctaacgccc gacttgaag 
        acgtcggcc atgagtgtt tcgaatgct ttgggcgcg 
        acggccatg aacgggatt ggaatctca agtacggcg 
        ggttcggct ccgacggcg atgtacagg cccaacttc 
        tcgcaagcg tttcaaagg gccgggccc taccccctg 
        tttccaggg gccaaccct ttcggcaca ggaacggga 
        ttactaaca aatggtcca gcagacttg agtatgaag 
        cagaagccg ctgttgaat cacaagctg agcactggg 
        gagatggcg gcggtgagg cagctgatt acggggtac 
        cgcgagtcg gcggcgttc ctgctgagg tcggcagac 
        gagttggag cagctcctg attcagcag cagtag
      • >TC031212 RB
        atgtcggcc aaacgtaag cccagtgcc attatccag 
        caccataag gagagtctg tccacgccg gagtcgtcc 
        gaagcgaac gaggaggac cactttgcg ggaaccggg 
        gctatgaaa gacagcgac aagctcaag ctgatgctc 
        ttggcgtgg aactaccac aatcaggcc caagggaac 
        agaaatggg gacatcccg gacctgtcc tcggttccg 
        ggaggcgag gaaatggcc ggcctgtgg gcccagtac 
        cactcggct ctggggcta gcgaaaggc aaggccacg 
        ccgcccacc actcgagat caatctagt ccagtcaat 
        gctgatacg gggtcagcc catgacgaa accagctcc 
        agcggtaac aaagaagac gaagacgat gacgatgat 
        gaaagcgat gacaagatc gacacacaa acgcacgac 
        cctgaacga ctcaaagcc ttcaatatg ttcgtccgt 
        ctgttcgtc gacgaaaac ctggaccgc atggtgccg 
        attagcaaa cagccgaaa gaaaagatc caagcaata 
        atcgattcg tgcacgagg cagtttccg gagttcgcg 
        gaacgagcc cggaagcgg atccgtact tacctcaag 
        tcttgtaga agaaacaaa cgtgccaga gacccgaac 
        tcgccatgg gacgcgacg cgaccgact ccagctcac 
        ttgacatca gtacaagca gaacaaatt ttagcaacg 
        gcctgcgag aacgaaagc cacaatgcg aaaagaatg 
        cgattgggg ctagaaccg gtcagtcag cccatgcca 
        atattaccg ggggcccag cctacggac accacagcc 
        ccgatcaac agaggaata gacttttcg aataacact 
        ccagtgaag atcgaggtg gactcggtc tcgacggcc 
        ttcccgtcg tcgacgacg ccctcctcc acccccaac 
        tcggtctcc aagctgtct ctaacgccc gacttgaag 
        acgtcggcc atgagtgtt tcgaatgct ttgggcgcg 
        acggccatg aacgggatt ggaatctca agtacggcg 
        ggttcggct ccgacggcg atgtacagg cccaacttc 
        tcgcaagcg tttcaaagg gccgggccc taccccctg 
        tttccaggg gccaaccct ttcggcaca ggaacggga 
        ttactaaca aatgacttg agtatgaag cagaagccg 
        ctgttgaat cacaagctg agcactggg gagatggcg 
        gcggtgagg cagctgatt acggggtac cgcgagtcg 
        gcggcgttc ctgctgagg tcggcagac gagttggag 
        cagctcctg attcagcag cagtag
      • >TC031212 RC
        atgctcgcc gaccgagga gtgcgccgc aagatggcc 
        caggaggag gcccgcccc aagaaggcg cccaaacgc 
        aagccgccc acgcccgcg cccaagagc cgcgccgag 
        cagaacaac aacgtcaag aggcagcgc ctctccgtc 
        gacaacaag gacgacatg ctggccacc taccagccc 
        tgggtcatc cagacctac ggcgacctg gccaagaca 
        aagaccatc acgttgaag aagtacgcg cggatcttg 
        cgcaccctc cgcggagag gagatcgtg agcgcggac 
        aactccaag tttcggttc tgggttaag gcgaagggc 
        ttccacgtg ggcaagccg gcgggctac gaggccaaa 
        cccgccgac gcgatcgtg ggacgatac agtgtgtgc 
        gacgccgac gaggggatt cccgcagtt aacgagaag 
        ttggcagga tcggccaag gatccgccg ctctacgtt 
        cccaatgcg cccttcaag aactctacc ggagagcca 
        atctatcgc aaagtcgcc gtcgtggag aacttcttc 
        gacatcatc tacaacgtc cacgtggaa ctggagggc 
        cgtcccggc aagcacgcc ggtcagaag cgaacctac 
        cgtacgata acggagaca tacgcgttc ctgccgcgc 
        gaagccgtc acccgcttc ctcctcggt tgcaccgag 
        tgtcagaaa catccccgg tccccatcg ccggcctcc 
        gttctgccc actccgagt cccagcccc acactgccc 
        ctggtccac acccccgcg cctcccttg ccgacgccg 
        cccccggcg ccgcccgcc gaggcccct taccgggtg 
        cccccggcc gccgaaagc ctcgacctc tcccggagc 
        aacggggag gtgcagaag cccgagtcg cggaccggc 
        acgccggag ccgaagcgc tcgagcaac cccctggac 
        gtctgcaac ctcacggcg aaggacccg ccgaagacg 
        cccccgaag aagcggcat ctcaacggc agcgagccc 
        aagcagacg ccgcccaag ctctggtcg ccggtggag 
        agcatcgag agggagcag caggagtcc aggaagcgg 
        agggtgctc atggcaggg gacatcgat tactcgctg 
        cctatcacc accacgtat ctcaagtat atgcgcagc 
        ttgggatgc acggacgag gacgccctc aagttcgaa 
        aacaaacac gagagtctg tccacgccg gagtcgtcc 
        gaagcgaac gaggaggac cactttgcg ggaaccggg 
        gctatgaaa gacagcgac aagctcaag ctgatgctc 
        ttggcgtgg aactaccac aatcaggcc caagggaac 
        agaaatggg gacatcccg gacctgtcc tcggttccg 
        ggaggcgag gaaatggcc ggcctgtgg gcccagtac 
        cactcggct ctggggcta gcgaaaggc aaggccacg 
        ccgcccacc actcgagat caatctagt ccagtcaat 
        gctgatacg gggtcagcc catgacgaa accagctcc 
        agcggtaac aaagaagac gaagacgat gacgatgat 
        gaaagcgat gacaagatc gacacacaa acgcacgac 
        cctgaacga ctcaaagcc ttcaatatg ttcgtccgt 
        ctgttcgtc gacgaaaac ctggaccgc atggtgccg 
        attagcaaa cagccgaaa gaaaagatc caagcaata 
        atcgattcg tgcacgagg cagtttccg gagttcgcg 
        gaacgagcc cggaagcgg atccgtact tacctcaag 
        tcttgtaga agaaacaaa cgtgccaga gacccgaac 
        tcgccatgg gacgcgacg cgaccgact ccagctcac 
        ttgacatca gtacaagca gaacaaatt ttagcaacg 
        gcctgcgag aacgaaagc cacaatgcg aaaagaatg 
        cgattgggg ctagaaccg gtcagtcag cccatgcca 
        atattaccg ggggcccag cctacggac accacagcc 
        ccgatcaac agaggaata gacttttcg aataacact 
        ccagtgaag atcgaggtg gactcggtc tcgacggcc 
        ttcccgtcg tcgacgacg ccctcctcc acccccaac 
        tcggtctcc aagctgtct ctaacgccc gacttgaag 
        acgtcggcc atgagtgtt tcgaatgct ttgggcgcg 
        acggccatg aacgggatt ggaatctca agtacggcg 
        ggttcggct ccgacggcg atgtacagg cccaacttc 
        tcgcaagcg tttcaaagg gccgggccc taccccctg 
        tttccaggg gccaaccct ttcggcaca ggaacggga 
        ttactaaca aatggtcca gcagacttg agtatgaag 
        cagaagccg ctgttgaat cacaagctg agcactggg 
        gagatggcg gcggtgagg cagctgatt acggggtac 
        cgcgagtcg gcggcgttc ctgctgagg tcggcagac 
        gagttggag cagctcctg attcagcag cagtag
      • >TC031212 RD
        atgctcgcc gaccgagga gtgcgccgc aagatggcc 
        caggaggag gcccgcccc aagaaggcg cccaaacgc 
        aagccgccc acgcccgcg cccaagagc cgcgccgag 
        cagaacaac aacgtcaag aggcagcgc ctctccgtc 
        gacaacaag gacgacatg ctggccacc taccagccc 
        tgggtcatc cagacctac ggcgacctg gccaagaca 
        aagaccatc acgttgaag aagtacgcg cggatcttg 
        cgcaccctc cgcggagag gagatcgtg agcgcggac 
        aactccaag tttcggttc tgggttaag gcgaagggc 
        ttccacgtg ggcaagccg gcgggctac gaggccaaa 
        cccgccgac gcgatcgtg ggacgatac agtgtgtgc 
        gacgccgac gaggggatt cccgcagtt aacgagaag 
        ttggcagga tcggccaag gatccgccg ctctacgtt 
        cccaatgcg cccttcaag aactctacc ggagagcca 
        atctatcgc aaagtcgcc gtcgtggag aacttcttc 
        gacatcatc tacaacgtc cacgtggaa ctggagggc 
        cgtcccggc aagcacgcc ggtcagaag cgaacctac 
        cgtacgata acggagaca tacgcgttc ctgccgcgc 
        gaagccgtc acccgcttc ctcctcggt tgcaccgag 
        tgtcagaaa catccccgg tccccatcg ccggcctcc 
        gttctgccc actccgagt cccagcccc acactgccc 
        ctggtccac acccccgcg cctcccttg ccgacgccg 
        cccccggcg ccgcccgcc gaggcccct taccgggtg 
        cccccggcc gccgaaagc ctcgacctc tcccggagc 
        aacggggag gtgcagaag cccgagtcg cggaccggc 
        acgccggag ccgaagcgc tcgagcaac cccctggac 
        gtctgcaac ctcacggcg aaggacccg ccgaagacg 
        cccccgaag aagcggcat ctcaacggc agcgagccc 
        aagcagacg ccgcccaag ctctggtcg ccggtggag 
        agcatcgag agggagcag caggagtcc aggaagcgg 
        agggtgctc atggcaggg gacatcgat tactcgctg 
        cctatcacc accacgtat ctcaagtat atgcgcagc 
        ttgggatgc acggacgag gacgccctc aagttcgaa 
        aacaaacac gtgagtctt gttattgaa cacttcttg 
        catgaggcc atctcgcac tatttttac tga
      mRNA sequence
      • >TC031212 RA
        acggatgta cgttgcaac aaaaatcga aacaatcgc 
        actgacaaa acaaccgaa attcagatc cggccgact 
        ccggggtga gagtgcgga gtgcgagct tttttccgc 
        gccgccttg atcggcgct ccgaaattc ggcaggccc 
        ggcgtgacg tcatcagcc tgacctatc gcaacatta 
        gaaacagtt agttggtca cggacgctt gcaaggatg 
        gccagattt gtgcgtgtg ttttgaggg ccagggccg 
        gctgcggcg aagatgtcg gccaaacgt aagcccagt 
        gccattatc cagcaccat aaggagagt ctgtccacg 
        ccggagtcg tccgaagcg aacgaggag gaccacttt 
        gcgggaacc ggggctatg aaagacagc gacaagctc 
        aagctgatg ctcttggcg tggaactac cacaatcag 
        gcccaaggg aacagaaat ggggacatc ccggacctg 
        tcctcggtt ccgggaggc gaggaaatg gccggcctg 
        tgggcccag taccactcg gctctgggg ctagcgaaa 
        ggcaaggcc acgccgccc accactcga gatcaatct 
        agtccagtc aatgctgat acggggtca gcccatgac 
        gaaaccagc tccagcggt aacaaagaa gacgaagac 
        gatgacgat gatgaaagc gatgacaag atcgacaca 
        caaacgcac gaccctgaa cgactcaaa gccttcaat 
        atgttcgtc cgtctgttc gtcgacgaa aacctggac 
        cgcatggtg ccgattagc aaacagccg aaagaaaag 
        atccaagca ataatcgat tcgtgcacg aggcagttt 
        ccggagttc gcggaacga gcccggaag cggatccgt 
        acttacctc aagtcttgt agaagaaac aaacgtgcc 
        agagacccg aactcgcca tgggacgcg acgcgaccg 
        actccagct cacttgaca tcagtacaa gcagaacaa 
        attttagca acggcctgc gagaacgaa agccacaat 
        gcgaaaaga atgcgattg gggctagaa ccggtcagt 
        cagcccatg ccaatatta ccgggggcc cagcctacg 
        gacaccaca gccccgatc aacagagga atagacttt 
        tcgaataac actccagtg aagatcgag gtggactcg 
        gtctcgacg gccttcccg tcgtcgacg acgccctcc 
        tccaccccc aactcggtc tccaagctg tctctaacg 
        cccgacttg aagacgtcg gccatgagt gtttcgaat 
        gctttgggc gcgacggcc atgaacggg attggaatc 
        tcaagtacg gcgggttcg gctccgacg gcgatgtac 
        aggcccaac ttctcgcaa gcgtttcaa agggccggg 
        ccctacccc ctgtttcca ggggccaac cctttcggc 
        acaggaacg ggattacta acaaatggt ccagcagac 
        ttgagtatg aagcagaag ccgctgttg aatcacaag 
        ctgagcact ggggagatg gcggcggtg aggcagctg 
        attacgggg taccgcgag tcggcggcg ttcctgctg 
        aggtcggca gacgagttg gagcagctc ctgattcag 
        cagcagtag actgttagt caaatgcca tatttgtgt 
        acattgcaa agatgatag gagagtatt ttatccact 
        aggcacgag tgattttag cactagtac tatgatact 
        gttgattca ctatgcatt cattaactt ggactgttg 
        taaactttg taaatatga tttttactt atggatttt 
        tgctactta cgaagagga ggaagatgc tgaatttat 
        gtacaaacg agtttccta aataagtga aaaatattt 
        atttattag aagttagaa gaactattc tattactac 
        aggggaaat tttattata tttgatata aatgttgag 
        aatttattg tatgaattg actgtacca aagacaatt 
        gtatataat ctttaaaaa gtcgccgat gtttttgta 
        attattgat gataaaatt catttagga atgtattgt 
        ctttcgagt gatgattcc aaattacgg ggtccccac 
        aaaaaaccc ctgacggac tttagtaca cctctaagg 
        gatcctctg caaattttt ataatagcg atattttct 
        attatttga cgattgaca tagaatttt aaagaagta 
        tttctgtga ttgttagta agtgtgtag atatactga 
        aaaatcagg aaacatctt acaaatttt ttgtaatct 
        atgtatctg ttcatgtat atttcagac attccaatg 
        tatttctga atgtatata attaaattg tattttgat 
        ctgaaatca ctgccagat tttgtgtat caataaata 
        atgtccgta tta
      • >TC031212 RB
        acggatgta cgttgcaac aaaaatcga aacaatcgc 
        actgacaaa acaaccgaa attcagatc cggccgact 
        ccggggtga gagtgcgga gtgcgagct tttttccgc 
        gccgccttg atcggcgct ccgaaattc ggcaggccc 
        ggcgtgacg tcatcagcc tgacctatc gcaacatta 
        gaaacagtt agttggtca cggacgctt gcaaggatg 
        gccagattt gtgcgtgtg ttttgaggg ccagggccg 
        gctgcggcg aagatgtcg gccaaacgt aagcccagt 
        gccattatc cagcaccat aaggagagt ctgtccacg 
        ccggagtcg tccgaagcg aacgaggag gaccacttt 
        gcgggaacc ggggctatg aaagacagc gacaagctc 
        aagctgatg ctcttggcg tggaactac cacaatcag 
        gcccaaggg aacagaaat ggggacatc ccggacctg 
        tcctcggtt ccgggaggc gaggaaatg gccggcctg 
        tgggcccag taccactcg gctctgggg ctagcgaaa 
        ggcaaggcc acgccgccc accactcga gatcaatct 
        agtccagtc aatgctgat acggggtca gcccatgac 
        gaaaccagc tccagcggt aacaaagaa gacgaagac 
        gatgacgat gatgaaagc gatgacaag atcgacaca 
        caaacgcac gaccctgaa cgactcaaa gccttcaat 
        atgttcgtc cgtctgttc gtcgacgaa aacctggac 
        cgcatggtg ccgattagc aaacagccg aaagaaaag 
        atccaagca ataatcgat tcgtgcacg aggcagttt 
        ccggagttc gcggaacga gcccggaag cggatccgt 
        acttacctc aagtcttgt agaagaaac aaacgtgcc 
        agagacccg aactcgcca tgggacgcg acgcgaccg 
        actccagct cacttgaca tcagtacaa gcagaacaa 
        attttagca acggcctgc gagaacgaa agccacaat 
        gcgaaaaga atgcgattg gggctagaa ccggtcagt 
        cagcccatg ccaatatta ccgggggcc cagcctacg 
        gacaccaca gccccgatc aacagagga atagacttt 
        tcgaataac actccagtg aagatcgag gtggactcg 
        gtctcgacg gccttcccg tcgtcgacg acgccctcc 
        tccaccccc aactcggtc tccaagctg tctctaacg 
        cccgacttg aagacgtcg gccatgagt gtttcgaat 
        gctttgggc gcgacggcc atgaacggg attggaatc 
        tcaagtacg gcgggttcg gctccgacg gcgatgtac 
        aggcccaac ttctcgcaa gcgtttcaa agggccggg 
        ccctacccc ctgtttcca ggggccaac cctttcggc 
        acaggaacg ggattacta acaaatgac ttgagtatg 
        aagcagaag ccgctgttg aatcacaag ctgagcact 
        ggggagatg gcggcggtg aggcagctg attacgggg 
        taccgcgag tcggcggcg ttcctgctg aggtcggca 
        gacgagttg gagcagctc ctgattcag cagcagtag 
        actgttagt caaatgcca tatttgtgt acattgcaa 
        agatgatag gagagtatt ttatccact aggcacgag 
        tgattttag cactagtac tatgatact gttgattca 
        ctatgcatt cattaactt ggactgttg taaactttg 
        taaatatga tttttactt atggatttt tgctactta 
        cgaagagga ggaagatgc tgaatttat gtacaaacg 
        agtttccta aataagtga aaaatattt atttattag 
        aagttagaa gaactattc tattactac aggggaaat 
        tttattata tttgatata aatgttgag aatttattg 
        tatgaattg actgtacca aagacaatt gtatataat 
        ctttaaaaa gtcgccgat gtttttgta attattgat 
        gataaaatt catttagga atgtattgt ctttcgagt 
        gatgattcc aaattacgg ggtccccac aaaaaaccc 
        ctgacggac tttagtaca cctctaagg gatcctctg 
        caaattttt ataatagcg atattttct attatttga 
        cgattgaca tagaatttt aaagaagta tttctgtga 
        ttgttagta agtgtgtag atatactga aaaatcagg 
        aaacatctt acaaatttt ttgtaatct atgtatctg 
        ttcatgtat atttcagac attccaatg tatttctga 
        atgtatata attaaattg tattttgat ctgaaatca 
        ctgccagat tttgtgtat caataaata atgtccgta 
        tta
      • >TC031212 RC
        agtcgcagc tcactcgat ccgcgcgag cgttcgtaa 
        cttcacaag ttttaacgc gctatgggc gcagccccc 
        gctctctga gctctcagt gaccgaact cccagtgct 
        tggccatgg ccaccgcga tgctcgccg accgaggag 
        tgcgccgca agatggccc aggaggagg cccgcccca 
        agaaggcgc ccaaacgca agccgccca cgcccgcgc 
        ccaagagcc gcgccgagc agaacaaca acgtcaaga 
        ggcagcgcc tctccgtcg acaacaagg acgacatgc 
        tggccacct accagccct gggtcatcc agacctacg 
        gcgacctgg ccaagacaa agaccatca cgttgaaga 
        agtacgcgc ggatcttgc gcaccctcc gcggagagg 
        agatcgtga gcgcggaca actccaagt ttcggttct 
        gggttaagg cgaagggct tccacgtgg gcaagccgg 
        cgggctacg aggccaaac ccgccgacg cgatcgtgg 
        gacgataca gtgtgtgcg acgccgacg aggggattc 
        ccgcagtta acgagaagt tggcaggat cggccaagg 
        atccgccgc tctacgttc ccaatgcgc ccttcaaga 
        actctaccg gagagccaa tctatcgca aagtcgccg 
        tcgtggaga acttcttcg acatcatct acaacgtcc 
        acgtggaac tggagggcc gtcccggca agcacgccg 
        gtcagaagc gaacctacc gtacgataa cggagacat 
        acgcgttcc tgccgcgcg aagccgtca cccgcttcc 
        tcctcggtt gcaccgagt gtcagaaac atccccggt 
        ccccatcgc cggcctccg ttctgccca ctccgagtc 
        ccagcccca cactgcccc tggtccaca cccccgcgc 
        ctcccttgc cgacgccgc ccccggcgc cgcccgccg 
        aggcccctt accgggtgc ccccggccg ccgaaagcc 
        tcgacctct cccggagca acggggagg tgcagaagc 
        ccgagtcgc ggaccggca cgccggagc cgaagcgct 
        cgagcaacc ccctggacg tctgcaacc tcacggcga 
        aggacccgc cgaagacgc ccccgaaga agcggcatc 
        tcaacggca gcgagccca agcagacgc cgcccaagc 
        tctggtcgc cggtggaga gcatcgaga gggagcagc 
        aggagtcca ggaagcgga gggtgctca tggcagggg 
        acatcgatt actcgctgc ctatcacca ccacgtatc 
        tcaagtata tgcgcagct tgggatgca cggacgagg 
        acgccctca agttcgaaa acaaacacg agagtctgt 
        ccacgccgg agtcgtccg aagcgaacg aggaggacc 
        actttgcgg gaaccgggg ctatgaaag acagcgaca 
        agctcaagc tgatgctct tggcgtgga actaccaca 
        atcaggccc aagggaaca gaaatgggg acatcccgg 
        acctgtcct cggttccgg gaggcgagg aaatggccg 
        gcctgtggg cccagtacc actcggctc tggggctag 
        cgaaaggca aggccacgc cgcccacca ctcgagatc 
        aatctagtc cagtcaatg ctgatacgg ggtcagccc 
        atgacgaaa ccagctcca gcggtaaca aagaagacg 
        aagacgatg acgatgatg aaagcgatg acaagatcg 
        acacacaaa cgcacgacc ctgaacgac tcaaagcct 
        tcaatatgt tcgtccgtc tgttcgtcg acgaaaacc 
        tggaccgca tggtgccga ttagcaaac agccgaaag 
        aaaagatcc aagcaataa tcgattcgt gcacgaggc 
        agtttccgg agttcgcgg aacgagccc ggaagcgga 
        tccgtactt acctcaagt cttgtagaa gaaacaaac 
        gtgccagag acccgaact cgccatggg acgcgacgc 
        gaccgactc cagctcact tgacatcag tacaagcag 
        aacaaattt tagcaacgg cctgcgaga acgaaagcc 
        acaatgcga aaagaatgc gattggggc tagaaccgg 
        tcagtcagc ccatgccaa tattaccgg gggcccagc 
        ctacggaca ccacagccc cgatcaaca gaggaatag 
        acttttcga ataacactc cagtgaaga tcgaggtgg 
        actcggtct cgacggcct tcccgtcgt cgacgacgc 
        cctcctcca cccccaact cggtctcca agctgtctc 
        taacgcccg acttgaaga cgtcggcca tgagtgttt 
        cgaatgctt tgggcgcga cggccatga acgggattg 
        gaatctcaa gtacggcgg gttcggctc cgacggcga 
        tgtacaggc ccaacttct cgcaagcgt ttcaaaggg 
        ccgggccct accccctgt ttccagggg ccaaccctt 
        tcggcacag gaacgggat tactaacaa atggtccag 
        cagacttga gtatgaagc agaagccgc tgttgaatc 
        acaagctga gcactgggg agatggcgg cggtgaggc 
        agctgatta cggggtacc gcgagtcgg cggcgttcc 
        tgctgaggt cggcagacg agttggagc agctcctga 
        ttcagcagc agtagactg ttagtcaaa tgccatatt 
        tgtgtacat tgcaaagat gataggaga gtattttat 
        ccactaggc acgagtgat tttagcact agtactatg 
        atactgttg attcactat gcattcatt aacttggac 
        tgttgtaaa ctttgtaaa tatgatttt tacttatgg 
        atttttgct acttacgaa gaggaggaa gatgctgaa 
        tttatgtac aaacgagtt tcctaaata agtgaaaaa 
        tatttattt attagaagt tagaagaac tattctatt 
        actacaggg gaaatttta ttatatttg atataaatg 
        ttgagaatt tattgtatg aattgactg taccaaaga 
        caattgtat ataatcttt aaaaagtcg ccgatgttt 
        ttgtaatta ttgatgata aaattcatt taggaatgt 
        attgtcttt cgagtgatg attccaaat tacggggtc 
        cccacaaaa aacccctga cggacttta gtacacctc 
        taagggatc ctctgcaaa tttttataa tagcgatat 
        tttctatta tttgacgat tgacataga attttaaag 
        aagtatttc tgtgattgt tagtaagtg tgtagatat 
        actgaaaaa tcaggaaac atcttacaa attttttgt 
        aatctatgt atctgttca tgtatattt cagacattc 
        caatgtatt tctgaatgt atataatta aattgtatt 
        ttgatctga aatcactgc cagattttg tgtatcaat 
        aaataatgt ccgtatta
      • >TC031212 RD
        agtcgcagc tcactcgat ccgcgcgag cgttcgtaa 
        cttcacaag ttttaacgc gctatgggc gcagccccc 
        gctctctga gctctcagt gaccgaact cccagtgct 
        tggccatgg ccaccgcga tgctcgccg accgaggag 
        tgcgccgca agatggccc aggaggagg cccgcccca 
        agaaggcgc ccaaacgca agccgccca cgcccgcgc 
        ccaagagcc gcgccgagc agaacaaca acgtcaaga 
        ggcagcgcc tctccgtcg acaacaagg acgacatgc 
        tggccacct accagccct gggtcatcc agacctacg 
        gcgacctgg ccaagacaa agaccatca cgttgaaga 
        agtacgcgc ggatcttgc gcaccctcc gcggagagg 
        agatcgtga gcgcggaca actccaagt ttcggttct 
        gggttaagg cgaagggct tccacgtgg gcaagccgg 
        cgggctacg aggccaaac ccgccgacg cgatcgtgg 
        gacgataca gtgtgtgcg acgccgacg aggggattc 
        ccgcagtta acgagaagt tggcaggat cggccaagg 
        atccgccgc tctacgttc ccaatgcgc ccttcaaga 
        actctaccg gagagccaa tctatcgca aagtcgccg 
        tcgtggaga acttcttcg acatcatct acaacgtcc 
        acgtggaac tggagggcc gtcccggca agcacgccg 
        gtcagaagc gaacctacc gtacgataa cggagacat 
        acgcgttcc tgccgcgcg aagccgtca cccgcttcc 
        tcctcggtt gcaccgagt gtcagaaac atccccggt 
        ccccatcgc cggcctccg ttctgccca ctccgagtc 
        ccagcccca cactgcccc tggtccaca cccccgcgc 
        ctcccttgc cgacgccgc ccccggcgc cgcccgccg 
        aggcccctt accgggtgc ccccggccg ccgaaagcc 
        tcgacctct cccggagca acggggagg tgcagaagc 
        ccgagtcgc ggaccggca cgccggagc cgaagcgct 
        cgagcaacc ccctggacg tctgcaacc tcacggcga 
        aggacccgc cgaagacgc ccccgaaga agcggcatc 
        tcaacggca gcgagccca agcagacgc cgcccaagc 
        tctggtcgc cggtggaga gcatcgaga gggagcagc 
        aggagtcca ggaagcgga gggtgctca tggcagggg 
        acatcgatt actcgctgc ctatcacca ccacgtatc 
        tcaagtata tgcgcagct tgggatgca cggacgagg 
        acgccctca agttcgaaa acaaacacg tgagtcttg 
        ttattgaac acttcttgc atgaggcca tctcgcact 
        atttttact gacgagttt cagttgact aaagttttt 
        aggtttag
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