Status
Please wait ...
Gene information
General information
TC033794
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010006
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10435154..10442737
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033794
    iBeetle screen iB_07343

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033794 RA
      MDRQQLQKF VQYLISEHH TEVLPTAQK LADEILQQR 
      SEINQIPGA PDPTAGASA DDEHSWHLD EEQVCEQVK 
      SYLSQGGYY NANKQLNSL FSKVREMLR AQDSNGARM 
      LTLITEQFL SDPRLQMWR NQGTPMTDK CRQLWDQLG 
      SLWVCIVLN PNCTQHEKQ QWKVLLEKW TLIDVCPPE 
      DPDFRLQDH SHRDSYISR DRDRDRDRY YHERDRDRY 
      ADRERDRRD RRDRDRERE RDRNRAAYD SSDSSEDDD 
      GDSSYSPRN SKRLRLSGS HSKSSVPTL PRTIFHRAL 
      DAVGMSWDN MHLKNILSS DTYCSHVPD TTSSGSFNS 
      QGQPLWHEP IPLCAARVD SLRSHGHME AALRLAVSV 
      VRTMKQQQL VAQRRWHES QQASTSSSS NSKCQTSCK 
      TTPCTSRCT GQCNSKSCS TTSNTAPAN TDCWVGHPL 
      DPIGTLFDT LADSSIIPD DQRPRTPSY LDIVGMEEQ 
      HNIRPRYHH VPVVGSRDR SETYLTLAF EVALIGLGQ 
      QRIMPVGLY AQEKACKQE DRLIAKLQE IELDNSLIA 
      VLRRQAIIL LEGGPTSGL GIGIHPESI PMHTFARFL 
      FLSLLSCYP DLAYEVGLR AMRLPLLEE HEDSDDPLG 
      GNSSSLMMR GSPRWFTLG HIETQQCAL SSTMLSAAK 
      GEVMRLRTV LESSQRHIH SSSHLFKLA QDAFRFATP 
      ENGPRHPTL LSVAFELGL QVSNSWKVT EVVVIFLCQ 
      VMRMTLSSL NWRRREMVR WLVTCATEV GVDALISIM 
      QNWYQFFTA TEATGPVAT TIMSHSTIM RLNLSFSQQ 
      EELSSCART LALQCATKD PPNCALNAL TLCENEALA 
      FETAYQIVV DAASHIMTS SQLFTIARY MEHRGYPTR 
      AYKLAMLAM KNVHLAYNQ DTHPAINDI HWACALSHS 
      LGKTELTNM IPLLVKNVQ CATVLSDIL RRCSMTAPG 
      ISCSHAPPL ETGKHHSHP HRSRSCAPG IKPLSYDRP 
      PLRQLLEAA VAAYINTTH SRLTHISPR HYGDFIDFL 
      TKARDTFVL AHDGHAQFT QLIENMKVA YKGKKKLMF 
      LVKERFG
    CDS sequence
    • >TC033794 RA
      atggatcgt caacagctt cagaaattt gtacagtat 
      ttgatatcg gagcaccat acggaagtg ctgccaacc 
      gcccaaaaa ctagccgat gaaatttta cagcagcgc 
      tccgaaatt aatcagatc cctggcgcc cccgatccg 
      accgccggc gcgtcagcg gatgatgaa cactcttgg 
      catttggac gaagaacaa gtatgcgaa caagtcaag 
      tcctatctg tcacaaggc ggttattac aacgccaac 
      aaacaactc aactcgtta ttttcaaag gtgcgtgaa 
      atgctacgc gctcaagat tccaacggg gcgagaatg 
      ttgacgttg attactgaa cagttcctc tccgatccc 
      cggttgcaa atgtggcgt aaccaaggt acaccaatg 
      actgataaa tgtagacaa ttgtgggat caactgggt 
      tccttatgg gtatgcatc gttcttaat ccgaattgt 
      acccaacac gagaaacag caatggaaa gtactgttg 
      gagaagtgg acgttgatt gacgtctgt ccgcccgaa 
      gatcccgat tttaggtta caggatcac tcgcatcgg 
      gattcgtat atcagtagg gacagggat agggataga 
      gatcggtac tatcacgag agggatagg gaccggtac 
      gccgatagg gaacgggat aggagggat cgcagggat 
      cgggacagg gagagggaa agagacaga aatcgagcc 
      gcttacgat tcgtcagat agctcggaa gacgacgat 
      ggcgacagt tcgtacagt cctagaaat agtaaacga 
      ttacgatta tcagggagt cattctaag tcaagcgta 
      ccaacgtta ccaaggaca atttttcac agggcgtta 
      gatgcggtg ggcatgtct tgggataac atgcactta 
      aaaaacatt ttatctagt gatacatac tgttcacat 
      gttcctgac acaactagt tccggcagt tttaattca 
      caaggccag cctttatgg cacgaaccg ataccatta 
      tgtgcggct cgagtcgac tccttgcgt tcccacggt 
      cacatggag gccgcttta cgactggcg gtttctgta 
      gtacgtacg atgaagcaa cagcaattg gttgcgcaa 
      cgtcgttgg cacgagagt cagcaagcc agcacgagt 
      agcagtagc aatagcaaa tgccaaacg agttgcaaa 
      acgacgccg tgtacgtcc cgatgcacg gggcagtgt 
      aatagcaag tcgtgttcg accacctcg aacacagct 
      cctgccaac acggattgt tgggtggga catcccttg 
      gaccccatc ggtactctt ttcgatacg cttgccgat 
      tcttcgatc attccagac gaccaaagg cctcgtact 
      ccgagttat ctcgatatc gtagggatg gaagaacag 
      cacaacatc aggccgagg tatcaccat gtgcctgtg 
      gtggggtcc cgggaccgg tccgaaacc tacctaact 
      ttggcgttc gaagtggct ttgatcggt ctagggcaa 
      cagaggata atgccagtg gggttgtac gcacaggag 
      aaggcgtgc aaacaggag gaccggctc atagccaag 
      ctgcaggaa atcgagttg gataacagt ctcatagcc 
      gtgcttcga cgccaagcc attattctc cttgaagga 
      ggcccaact agtggccta gggatcggt attcaccct 
      gagagtatt ccaatgcac accttcgct cggtttctt 
      tttctgtcg cttctgagc tgttacccg gacctggcc 
      tacgaagtc ggtttgcga gcgatgcga ctccccctg 
      ctggaggag cacgaggat tcggatgac cctcttggg 
      ggcaatagc agttcgttg atgatgagg ggctctcca 
      aggtggttc actttgggt cacattgaa acgcagcaa 
      tgcgctctc agttcgacg atgctcagt gcggccaag 
      ggcgaagtt atgagactg cgaacggtg ctggaatcg 
      tcccagagg catatccac agttcttcc catttgttt 
      aaacttgct caagacgcg tttcggttt gcgacgcct 
      gagaatgga cccagacat cccaccttg ctgagtgtg 
      gcctttgag ttgggcctg caggtaagt aattcttgg 
      aaggtgaca gaagttgta gtaattttt ctgtgtcag 
      gttatgagg atgactttg tcgtctcta aactggcgg 
      cgacgtgag atggtgaga tggctggtg acttgtgcg 
      acggaagtg ggagtcgac gcgcttatt tcaattatg 
      cagaattgg tatcagttt tttacagcg actgaagca 
      accggccct gttgctacc acaataatg tcacatagt 
      accattatg cgtcttaat cttagtttt agtcaacaa 
      gaagaattg tccagttgt gcgagaacg ttagctctg 
      caatgtgca actaaggac cctccaaat tgtgccttg 
      aacgcgttg acgttgtgc gagaacgag gctctggcc 
      tttgagacc gcttaccag attgtcgtg gatgcagcc 
      tcacacatc atgacatct tcgcaactc tttaccatt 
      gccagatac atggaacat aggggctat ccaacccgg 
      gcctacaag ctagccatg ttggctatg aaaaatgtt 
      catctcgct tacaatcag gatacccat ccagcaata 
      aacgacatc cactgggcg tgcgccttg agccattcg 
      ttgggcaaa accgaactg acaaacatg atccccctc 
      ctcgttaaa aacgttcag tgcgccacc gtgttgtcg 
      gacattctc cgtcggtgc agcatgacc gctccgggc 
      atctcctgc tcccatgcg cccccttta gaaaccgga 
      aaacaccat tcgcatccg caccggagt cgaagctgc 
      gctcccgga atcaagcca ctgtcgtac gacagaccc 
      cccttgcgg cagctgctg gaggccgcc gtggccgcc 
      tacatcaac accacgcat tcacggcta acacatatc 
      tcgccgagg cattacggc gatttcata gacttttta 
      acaaaggcc agagacacg ttcgtgttg gcgcacgat 
      ggccacgcg cagtttacg caattaatc gagaacatg 
      aaggtggcg tacaaaggc aagaagaaa ctcatgttt 
      ctggtaaaa gagaggttc ggctga
    mRNA sequence
    • >TC033794 RA
      ttgtttcag aaactcttt tgcaaatgg atcgtcaac 
      agcttcaga aatttgtac agtatttga tatcggagc 
      accatacgg aagtgctgc caaccgccc aaaaactag 
      ccgatgaaa ttttacagc agcgctccg aaattaatc 
      agatccctg gcgcccccg atccgaccg ccggcgcgt 
      cagcggatg atgaacact cttggcatt tggacgaag 
      aacaagtat gcgaacaag tcaagtcct atctgtcac 
      aaggcggtt attacaacg ccaacaaac aactcaact 
      cgttatttt caaaggtgc gtgaaatgc tacgcgctc 
      aagattcca acggggcga gaatgttga cgttgatta 
      ctgaacagt tcctctccg atccccggt tgcaaatgt 
      ggcgtaacc aaggtacac caatgactg ataaatgta 
      gacaattgt gggatcaac tgggttcct tatgggtat 
      gcatcgttc ttaatccga attgtaccc aacacgaga 
      aacagcaat ggaaagtac tgttggaga agtggacgt 
      tgattgacg tctgtccgc ccgaagatc ccgatttta 
      ggttacagg atcactcgc atcgggatt cgtatatca 
      gtagggaca gggataggg atagagatc ggtactatc 
      acgagaggg atagggacc ggtacgccg atagggaac 
      gggatagga gggatcgca gggatcggg acagggaga 
      gggaaagag acagaaatc gagccgctt acgattcgt 
      cagatagct cggaagacg acgatggcg acagttcgt 
      acagtccta gaaatagta aacgattac gattatcag 
      ggagtcatt ctaagtcaa gcgtaccaa cgttaccaa 
      ggacaattt ttcacaggg cgttagatg cggtgggca 
      tgtcttggg ataacatgc acttaaaaa acattttat 
      ctagtgata catactgtt cacatgttc ctgacacaa 
      ctagttccg gcagtttta attcacaag gccagcctt 
      tatggcacg aaccgatac cattatgtg cggctcgag 
      tcgactcct tgcgttccc acggtcaca tggaggccg 
      ctttacgac tggcggttt ctgtagtac gtacgatga 
      agcaacagc aattggttg cgcaacgtc gttggcacg 
      agagtcagc aagccagca cgagtagca gtagcaata 
      gcaaatgcc aaacgagtt gcaaaacga cgccgtgta 
      cgtcccgat gcacggggc agtgtaata gcaagtcgt 
      gttcgacca cctcgaaca cagctcctg ccaacacgg 
      attgttggg tgggacatc ccttggacc ccatcggta 
      ctcttttcg atacgcttg ccgattctt cgatcattc 
      cagacgacc aaaggcctc gtactccga gttatctcg 
      atatcgtag ggatggaag aacagcaca acatcaggc 
      cgaggtatc accatgtgc ctgtggtgg ggtcccggg 
      accggtccg aaacctacc taactttgg cgttcgaag 
      tggctttga tcggtctag ggcaacaga ggataatgc 
      cagtggggt tgtacgcac aggagaagg cgtgcaaac 
      aggaggacc ggctcatag ccaagctgc aggaaatcg 
      agttggata acagtctca tagccgtgc ttcgacgcc 
      aagccatta ttctccttg aaggaggcc caactagtg 
      gcctaggga tcggtattc accctgaga gtattccaa 
      tgcacacct tcgctcggt ttctttttc tgtcgcttc 
      tgagctgtt acccggacc tggcctacg aagtcggtt 
      tgcgagcga tgcgactcc ccctgctgg aggagcacg 
      aggattcgg atgaccctc ttgggggca atagcagtt 
      cgttgatga tgaggggct ctccaaggt ggttcactt 
      tgggtcaca ttgaaacgc agcaatgcg ctctcagtt 
      cgacgatgc tcagtgcgg ccaagggcg aagttatga 
      gactgcgaa cggtgctgg aatcgtccc agaggcata 
      tccacagtt cttcccatt tgtttaaac ttgctcaag 
      acgcgtttc ggtttgcga cgcctgaga atggaccca 
      gacatccca ccttgctga gtgtggcct ttgagttgg 
      gcctgcagg taagtaatt cttggaagg tgacagaag 
      ttgtagtaa tttttctgt gtcaggtta tgaggatga 
      ctttgtcgt ctctaaact ggcggcgac gtgagatgg 
      tgagatggc tggtgactt gtgcgacgg aagtgggag 
      tcgacgcgc ttatttcaa ttatgcaga attggtatc 
      agtttttta cagcgactg aagcaaccg gccctgttg 
      ctaccacaa taatgtcac atagtacca ttatgcgtc 
      ttaatctta gttttagtc aacaagaag aattgtcca 
      gttgtgcga gaacgttag ctctgcaat gtgcaacta 
      aggaccctc caaattgtg ccttgaacg cgttgacgt 
      tgtgcgaga acgaggctc tggcctttg agaccgctt 
      accagattg tcgtggatg cagcctcac acatcatga 
      catcttcgc aactcttta ccattgcca gatacatgg 
      aacataggg gctatccaa cccgggcct acaagctag 
      ccatgttgg ctatgaaaa atgttcatc tcgcttaca 
      atcaggata cccatccag caataaacg acatccact 
      gggcgtgcg ccttgagcc attcgttgg gcaaaaccg 
      aactgacaa acatgatcc ccctcctcg ttaaaaacg 
      ttcagtgcg ccaccgtgt tgtcggaca ttctccgtc 
      ggtgcagca tgaccgctc cgggcatct cctgctccc 
      atgcgcccc ctttagaaa ccggaaaac accattcgc 
      atccgcacc ggagtcgaa gctgcgctc ccggaatca 
      agccactgt cgtacgaca gacccccct tgcggcagc 
      tgctggagg ccgccgtgg ccgcctaca tcaacacca 
      cgcattcac ggctaacac atatctcgc cgaggcatt 
      acggcgatt tcatagact ttttaacaa aggccagag 
      acacgttcg tgttggcgc acgatggcc acgcgcagt 
      ttacgcaat taatcgaga acatgaagg tggcgtaca 
      aaggcaaga agaaactca tgtttctgg taaaagaga 
      ggttcggct gatataact tgaaaaagt ggtacatgt 
      agtgtgtgc gtttttaac gcggatcgc cacgatgat 
      ggaagccta gcggtcttc aagcgcctg tactatatg 
      tagctcgct tcaattctt ttcatagtc taatctaac 
      tggacaata ttcatgaaa tgagatttt tttcgtatg 
      tatttattg actgtttat tcccccctg aacagactg 
      attttattg ttttatggg caaccgggc caaacttca 
      cgctggtca cgtgataat cataattat gtgtatatt 
      tttaggtaa taaataagt aagaggtca gcttgaact 
      ttgacgggt tgcactgga tctcatttg tacttgtag 
      tattatttt tattttcgt cagcatttc tatttttac 
      gttttaggg tctcgtaaa tccaggata taaattggt 
      aggaaaggc ataactagt ttttcgtat ttgtgcgca 
      gcttgcttt tttctaccg atgtgtact tggggtttg 
      ccaaacctt acgtttcct ctgtcccat gtcgctttt 
      ttagtattt aatgttaat ttgtttacc aaagattgt 
      tgtttgtat agtatttat gtacatata ccgtaagag 
      agacagtct cattagtta attgggaag catacttga 
      ggtgccgtt tgaacttcc tcgtgcgcc actcgttta 
      tttcacacc tatcaaggg atgctttcc acaaataca 
      aggtaaatg cttcctgtt atatttata taatacagt 
      atatcaata agtacaaaa taactctaa acacgcagt 
      cgaccaagc tgtagcgcg atgtcagct gtcattccg 
      taagctacc tctccgagc taaacctaa caacattca 
      accgcgctt tgttcatgt ttagtttta tttttcagg 
      ttgggtgtt tccaaattg actgaatta tacgagaaa 
      aatcaaatc gacccgctt agctctccc gtttttatt 
      tagaggcaa ccgatactt tcgacaaga tttcaaaaa 
      aatacgaaa atgtaacgt cgctgccgc aacatcttg 
      ttttgttgt ttttcgtgt ctgtgattg aatcttttt 
      atttaatga ttacgttcg tccagtgcc actaaaata 
      ataagttag tgaaaaaaa catcgttga cttttaagt 
      tttttcttt tggtattaa ggagatgta aatattgac 
      ttggccaaa ttggtacac gttccattc caataaaaa 
      tgattttat ataattcgt tgttgtatt gtgtttttt 
      tgtgtataa taatcatta ggaaaatgt acatattgc 
      caaaaacat tttagttct ttatttgtt aaaatgcaa 
      ttattgcgt tcagtctgt ctgtgtatt aataattta 
      tgattattt ttacgataa taaaaatat ttgtaattc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY