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Gene information
General information
TC009754 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7365137..7373014
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009754
    iBeetle screen iB_07342

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009754 RA
      MDEKHFRVN HLGENYNIY EFLNNHPGG INYVKPYEE 
      KEVTQRMQQ TQHSKAAYY LLREYKDGG RDSSNKGDQ 
      EDLEALVDW DKPMLAQVA NLGAKYNEW VISPVDRKL 
      RLFGNPILE NLTITPWYV VPLVWVPVI LYFIIHGTR 
      KYVQLTEDP SPFLPTVLS VVLGVVLWT LIEYSLHRW 
      VFHMEPSGK SKVMIYVHF AIHGLHHKV PFDPRRLVF 
      PPFPAAVIA YVLYEICTF FVPDSIIFL VIAGGLAGY 
      VIYDMIHFY LHYGSPKED SYFYYLKRY HNQHHFAHH 
      DSGFGISSV FWDKVFGSA IKLRQLAMA IKW
    • >TC009754 RB
      MSNEQVHVD IANSVMDEK HFRVNHLGE NYNIYEFLN 
      NHPGGINYV KPYEEKEVT QRMQQTQHS KAAYYLLRE 
      YKDGGRDSS NKGDQEDLE ALVDWDKPM LAQVANLGA 
      KYNEWVISP VDRKLRLFG NPILENLTI TPWYVVPLV 
      WVPVILYFI IHGTRKYVQ LTEDPSPFL PTVLSVVLG 
      VVLWTLIEY SLHRWVFHM EPSGKSKVM IYVHFAIHG 
      LHHKVPFDP RRLVFPPFP AAVIAYVLY EICTFFVPD 
      SIIFLVIAG GLAGYVIYD MIHFYLHYG SPKEDSYFY 
      YLKRYHNQH HFAHHDSGF GISSVFWDK VFGSAIKLR 
      QLAMAIKW
    CDS sequence
    • >TC009754 RA
      atggacgaa aaacacttc cgagttaac catttgggg 
      gagaattac aacatttac gagtttcta aataaccat 
      ccgggcggc ataaactat gtcaaaccc tacgaagag 
      aaagaagtc acacaaagg atgcaacaa acgcaacat 
      tcgaaagct gcctattat ttgttgaga gagtacaaa 
      gacggcgga cgcgatagt agtaataaa ggtgatcag 
      gaagactta gaagcacta gtggattgg gacaagccc 
      atgttggca caagtggcc aatttgggg gcgaaatac 
      aacgaatgg gtgatatct ccagttgat aggaagttg 
      aggttgttt ggtaaccca attttggag aatttaacc 
      atcacaccg tggtatgtg gtgccgctg gtttgggtg 
      ccagttatt ttgtatttt atcatccat gggactaga 
      aaatacgta cagctcacg gaagaccca tcccccttt 
      ctaccaaca gtattaagt gtagtacta ggagtggtt 
      ctgtggacc ctaatagag tactctctc caccgatgg 
      gttttccat atggagcca tccgggaaa tcaaaagta 
      atgatttat gtgcatttt gccattcac ggccttcac 
      cacaaggtg ccttttgat cctcgcaga ctagtattc 
      ccgccattt cctgccgct gtaatagca tacgtactt 
      tacgaaatt tgtacgttt ttcgttccc gattcgatt 
      attttttta gttatagca ggagggcta gcaggatac 
      gttatttac gacatgatt cacttttat ttacattac 
      ggttcgcct aaagaagac agctacttt tattatttg 
      aagcggtac cacaatcag caccatttt gcacatcat 
      gacagtggt tttggcatc agtagcgtc ttctgggac 
      aaggttttc ggatctgcg attaagctg aggcagctt 
      gctatggcc attaaatgg tga
    • >TC009754 RB
      atgagcaac gagcaagtg cacgtagat atagcaaat 
      agcgtcatg gacgaaaaa cacttccga gttaaccat 
      ttgggggag aattacaac atttacgag tttctaaat 
      aaccatccg ggcggcata aactatgtc aaaccctac 
      gaagagaaa gaagtcaca caaaggatg caacaaacg 
      caacattcg aaagctgcc tattatttg ttgagagag 
      tacaaagac ggcggacgc gatagtagt aataaaggt 
      gatcaggaa gacttagaa gcactagtg gattgggac 
      aagcccatg ttggcacaa gtggccaat ttgggggcg 
      aaatacaac gaatgggtg atatctcca gttgatagg 
      aagttgagg ttgtttggt aacccaatt ttggagaat 
      ttaaccatc acaccgtgg tatgtggtg ccgctggtt 
      tgggtgcca gttattttg tattttatc atccatggg 
      actagaaaa tacgtacag ctcacggaa gacccatcc 
      ccctttcta ccaacagta ttaagtgta gtactagga 
      gtggttctg tggacccta atagagtac tctctccac 
      cgatgggtt ttccatatg gagccatcc gggaaatca 
      aaagtaatg atttatgtg cattttgcc attcacggc 
      cttcaccac aaggtgcct tttgatcct cgcagacta 
      gtattcccg ccatttcct gccgctgta atagcatac 
      gtactttac gaaatttgt acgtttttc gttcccgat 
      tcgattatt tttttagtt atagcagga gggctagca 
      ggatacgtt atttacgac atgattcac ttttattta 
      cattacggt tcgcctaaa gaagacagc tacttttat 
      tatttgaag cggtaccac aatcagcac cattttgca 
      catcatgac agtggtttt ggcatcagt agcgtcttc 
      tgggacaag gttttcgga tctgcgatt aagctgagg 
      cagcttgct atggccatt aaatggtga
    mRNA sequence
    • >TC009754 RA
      ttccgcgta aaaaggact ccttgtcct aggttccgg 
      aaagctttc ggagtgcgt cagtttaat ctccctaag 
      ataagaaac gctgtgcaa atttatcag agactcggg 
      attttgtag caatgcaat gctgagttg tgtaggaat 
      taagacagt gtattttac caagcgtca tggacgaaa 
      aacacttcc gagttaacc atttggggg agaattaca 
      acatttacg agtttctaa ataaccatc cgggcggca 
      taaactatg tcaaaccct acgaagaga aagaagtca 
      cacaaagga tgcaacaaa cgcaacatt cgaaagctg 
      cctattatt tgttgagag agtacaaag acggcggac 
      gcgatagta gtaataaag gtgatcagg aagacttag 
      aagcactag tggattggg acaagccca tgttggcac 
      aagtggcca atttggggg cgaaataca acgaatggg 
      tgatatctc cagttgata ggaagttga ggttgtttg 
      gtaacccaa ttttggaga atttaacca tcacaccgt 
      ggtatgtgg tgccgctgg tttgggtgc cagttattt 
      tgtatttta tcatccatg ggactagaa aatacgtac 
      agctcacgg aagacccat ccccctttc taccaacag 
      tattaagtg tagtactag gagtggttc tgtggaccc 
      taatagagt actctctcc accgatggg ttttccata 
      tggagccat ccgggaaat caaaagtaa tgatttatg 
      tgcattttg ccattcacg gccttcacc acaaggtgc 
      cttttgatc ctcgcagac tagtattcc cgccatttc 
      ctgccgctg taatagcat acgtacttt acgaaattt 
      gtacgtttt tcgttcccg attcgatta tttttttag 
      ttatagcag gagggctag caggatacg ttatttacg 
      acatgattc acttttatt tacattacg gttcgccta 
      aagaagaca gctactttt attatttga agcggtacc 
      acaatcagc accattttg cacatcatg acagtggtt 
      ttggcatca gtagcgtct tctgggaca aggttttcg 
      gatctgcga ttaagctga ggcagcttg ctatggcca 
      ttaaatggt gaagagacg tacaaattg accaaaact 
      ttaatgtgt gttcgtcaa atgtgtgat tttttagtc 
      gtttattct tatgtaagt ttattgtta ttgaagact 
      tttcaagaa ttccaaata tattttttg atttatt
    • >TC009754 RB
      ttccgcgta aaaaggact ccttgtcct aggttccgg 
      aaagctttc ggagtgcgt cagtttaat ctccctaag 
      ataagaaac gctgtgcaa atttatcag agactcggg 
      attttgtag caatgcaat gctgagttg tgtaggaat 
      taagacagt gtattttac caatttttt gacttggtg 
      tgaatgagc aacgagcaa gtgcacgta gatatagca 
      aatagcgtc atggacgaa aaacacttc cgagttaac 
      catttgggg gagaattac aacatttac gagtttcta 
      aataaccat ccgggcggc ataaactat gtcaaaccc 
      tacgaagag aaagaagtc acacaaagg atgcaacaa 
      acgcaacat tcgaaagct gcctattat ttgttgaga 
      gagtacaaa gacggcgga cgcgatagt agtaataaa 
      ggtgatcag gaagactta gaagcacta gtggattgg 
      gacaagccc atgttggca caagtggcc aatttgggg 
      gcgaaatac aacgaatgg gtgatatct ccagttgat 
      aggaagttg aggttgttt ggtaaccca attttggag 
      aatttaacc atcacaccg tggtatgtg gtgccgctg 
      gtttgggtg ccagttatt ttgtatttt atcatccat 
      gggactaga aaatacgta cagctcacg gaagaccca 
      tcccccttt ctaccaaca gtattaagt gtagtacta 
      ggagtggtt ctgtggacc ctaatagag tactctctc 
      caccgatgg gttttccat atggagcca tccgggaaa 
      tcaaaagta atgatttat gtgcatttt gccattcac 
      ggccttcac cacaaggtg ccttttgat cctcgcaga 
      ctagtattc ccgccattt cctgccgct gtaatagca 
      tacgtactt tacgaaatt tgtacgttt ttcgttccc 
      gattcgatt attttttta gttatagca ggagggcta 
      gcaggatac gttatttac gacatgatt cacttttat 
      ttacattac ggttcgcct aaagaagac agctacttt 
      tattatttg aagcggtac cacaatcag caccatttt 
      gcacatcat gacagtggt tttggcatc agtagcgtc 
      ttctgggac aaggttttc ggatctgcg attaagctg 
      aggcagctt gctatggcc attaaatgg tgaagagac 
      gtacaaatt gaccaaaac tttaatgtg tgttcgtca 
      aatgtgtga ttttttagt cgtttattc ttatgtaag 
      tttattgtt attgaagac ttttcaaga attccaaat 
      atatttttt gatttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY