Status
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Gene information
General information
TC033221
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014674,TC014675
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13360503..13371367
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033221
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07339

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033221 RA
      MNPDEYDFR RNIPNKEPS SQALDFIRG LEDRSLNHL 
      KTNIRAKAS REVFAEDEL VRKKCYDEM RCEMPGQPR 
      STFLMVFSP DGTKVASTH GNHNIYVTD IKSGKNIKT 
      LTGHPRTPW CIAFHPTSN QIVASGCLG GEVRIWDLS 
      GGSEVWTAG NQSVIASIA FHPNDRILV IATYNELYF 
      WDWSMPEPF VQVATANPK EKIRYVAFD KLGHKLITG 
      IANSPQTRW ERVRAPVPV PRQAERSAS PYRLRITPR 
      LVNTPSPPE RTYFNVGLR PEQPSVVES TREVLSSVP 
      ERERRITAS YRNLLREYE QLVQRYLKV YKPPTMIDR 
      GTDPMEPNA TSATSSTQA PESEPSTSG TNPTESQPS 
      TSQNYSSSL VMPSRILAM KKQPTEAHS TQTSDSRKH 
      KSSENTEES TQEKRSKSS DSGPSGSSH TETATQTQS 
      PRRLLVINE RPQPEPSTS GLQDKPVRN SGVRILCPK 
      KRKLPEAGG SSSEPQPST SRGSTSGDI LQNIRPIAE 
      EVRNRFLPI IKSLPACDR PKLIRRFEN GRAHERLKL 
      RQMCPTFLK KPNRRPVLD TSSDSSSSD DERGGTLRS 
      RMPIDLTTN TTNQNRNAT NQNEPEPEC VHTASNSNP 
      PSNPSPSNS NNSNISGVR NFIMELEQL LMTVLLTEI 
      ESNESQQNS PSANNNNSP INLSNTSPV TRSQDEQAD 
      SNVRSSPVS MSASRDQDL IRPNAVPLA FSTFRRLIQ 
      RQENQESES RTPTSNSDE PSRSSPGSS NGIHSRTSS 
      PIYNRTYPR RRLPAHRIS AFMPTRVNY NRIPMRYRR 
      GSSFRLANR LSVNSTFPI DELINYSER PNAEDNVPQ 
      DNLPESSPL EPPTEPSPF VNLGNMYSN IVQDLESSL 
      NDVRNIRSS TRPSETSDM LSSFSESLE NIMNQSDTI 
      LRNLGGSMS MLPNYSEQN ETRRRETTE PRATFNDQN 
      FYIRSNSPQ EASTSTGER NLSEVLAGG RSVVESDHN 
      YPRNPGNNQ GPSSGENMS PLMTSLHLT ISYIQRQAR 
      LLRHQVESI ERIDRTMFE VEQLQLIRQ LITEFLRYV 
      RSLAGESRS TGMSSVRQM MAGTRISDS SPYDSQSEE 
      RPQQPPQPT PSSSTSTDQ SQPRPRTSG RKTYPPSRL 
      FRLQRHNRR SLFVNIFPR RYTGRPERG AKHTGPCRN 
      APSRTPEAT NPLRHLSSH LLINSSSLN IMARRLEHF 
      LTEQTRLIT RPQESGSGS IRSTAATEL GERILSIRL 
      YDCVMRMNR IMGFDSSRF RNSRSDLVV VTQDGASRF 
      PARHFLSLI VDAMSKHLE ELAGSNISQ SLRGQIHSV 
      LAMALLLSD LLLLQLVDS IPPPAGMNL DVERESLTS 
      RIDQMCGQM LQNRFSGHS HQLTRSLQH MRLNMRRAY 
      RALGQTYNA RRNAMLPNR PDTNRRQLL NRYLRNINL 
      RRHQNSTSS DTSANVAPS APAESENVT RGWSSLRDL 
      IMRYSDNTG EDEDVTSSN SNQDSQERT QRFETVSSN 
      NSDDNDDES EWYVNNNNR STNLYRTNN VNLVHNNPE 
      ESNPSRPWN VPSVQVNDV PVPIAEQPS LFQQRIMTH 
      RQRLAERVS ELRTFPQMG GLRPRFLHP LYASVNPFD 
      ADLDDPQRE TNNYDCDMI TTVTPNHRI QMWDISSGN 
      IPVINNPLK NLIVSECKI HNDASVDIA KDGTILVTL 
      LPSGGYLNV TNKLGVYSL RWDTLGQCL YTTSFEQNA 
      VSVALSPLS RHLVVGLAS RRVSIVPSD RWTMARIFI 
      IEQKNAPGD RLPVLRELG QNRDSRANY KSVNCIRWL 
      PTSGQGLIY ATNTGQLGK GNKRVNANG KFIPMMRPR 
      GPFPPPRGP MGMRPMMGP PHMRPPPPR GLRPPPPRL 
      PRGPIMPPR MMGPGPRPL PPPGMRPPP PHMMGPMRG 
      MGFRRGMPR KPPMGAFKG VQRGRVLKR RRPMIKDID 
      LTKEWVTDA IRQEFKKKE ELLAAAKSA ESKEAWATY 
      RTQREKCSQ MYHVAEMEF VGLQETTEQ DWDDFEYEE 
      NPESEVFFC DVCEEDFYT REHYENHMS QHRICGVDG 
      CQYTAPQKM IEKHIQMQH LTGLYEKIR SVNTPEEIA 
      KWIEERKVR YPSRENVQK RYERREEML KRGEKIGER 
      KNRFGKDKT RLSRVRHKK QQRKNVPKV VKNPEQAKQ 
      SLIDEKCDW NGTMFPFRG TKELYNEED EKETSDYDD 
      EEWMDGSGK VTVELNNAL GALMGAYES DQEEETPQT 
      VDNDAPTEE KIQREQVEY PNEQASDTK VVKTNKRRR 
      LSSGKKQKL NNKPEPGGF SRMKFVKRK VTLLEKLLD 
      SEIRHERNV LLQCVQHVV TNNFFR
    CDS sequence
    • >TC033221 RA
      atgaaccca gatgaatat gactttcgt cgaaatatc 
      cccaacaaa gagccctcc agccaggcc ctagacttc 
      atccggggc ctcgaagac aggagcctc aaccaccta 
      aagacaaac atcagagca aaagccagc cgagaagtt 
      ttcgccgaa gatgaactg gtcagaaaa aaatgctac 
      gacgaaatg cgctgcgag atgccaggc caacccaga 
      tccacattt ctcatggtc ttcagccca gatggaacc 
      aaagttgcc tccacccac ggcaaccac aacatctac 
      gtcacggat atcaaaagc gggaaaaac ataaagact 
      ctgacagga cacccgagg actccgtgg tgcattgcc 
      ttccaccca acctccaac caaattgtg gcgtctggc 
      tgcctggga ggggaagtc aggatttgg gatctgagt 
      ggaggtagc gaggtctgg actgccggg aatcaatcc 
      gtaattgcg tcaattgcg ttccatccc aatgaccgg 
      attttggtc attgcgaca tacaatgaa ctgtacttt 
      tgggactgg agcatgccg gagccgttc gtccaagtg 
      gcgactgcc aatcccaag gaaaagatt agatatgtg 
      gcgtttgat aagttgggc cataaattg attacgggg 
      attgcgaac agcccccag acgaggtgg gaacgggta 
      agggcccct gtccctgtc ccgagacaa gcagaaagg 
      tctgctagc ccctacaga cttagaatt acgccaaga 
      ctggtcaac acaccttca cccccagag aggacttat 
      tttaatgta ggcttacgt ccagagcaa cctagcgtt 
      gttgaaagc actcgtgag gtactttcc agtgttcct 
      gaacgagaa agacgcata acggcatct tatcgtaac 
      cttctaaga gagtacgaa caattggtc caacgctat 
      ttgaaagtt tacaaacct ccaactatg attgaccgg 
      ggcactgac cccatggaa cccaacgcc acgagtgcc 
      accagcagc actcaagcc cccgaatcc gaaccaagc 
      acttcggga acgaaccca actgaatct caaccttcc 
      acctcccaa aactacagt tcatccctt gtcatgcca 
      agccgtatt ttagcaatg aaaaaacaa ccaactgaa 
      gctcacagc acccaaact tcagactcg agaaaacat 
      aaatcgtcc gaaaataca gaggaatcg actcaagaa 
      aaacgaagt aaatcaagc gattctggc ccaagtggg 
      tcgtcacat actgaaaca gcgacacaa acccaaagt 
      cccagaaga ctgctagtg ataaatgaa aggccccaa 
      cccgaacct tcaacaagt gggctgcag gacaaacct 
      gttagaaat tcaggagtg cggattttg tgcccgaaa 
      aaacgcaag ttaccagaa gctgggggc tcgtctagc 
      gagccccag ccgagcaca agtcggggg agcacttcg 
      ggggatatt ctgcaaaat attcgcccc attgccgaa 
      gaagtgcgg aatcggttc ttgcccatt atcaagtcg 
      ctccccgcc tgtgaccga ccgaaatta attcgacgt 
      tttgagaac gggcgggct catgaaaga ctgaagttg 
      agacaaatg tgtccaact tttttgaaa aagccgaat 
      cggaggcca gtccttgac acctcttct gatagtagt 
      agttctgac gacgaacga ggtggcact ttacgttca 
      cgaatgccc atagatctc acaacaaat acaactaat 
      caaaataga aatgcaacc aatcaaaat gagcctgaa 
      ccagaatgt gttcatacc gccagcaat agtaaccct 
      ccaagcaac ccttcacct tcaaactca aacaattca 
      aacatcagt ggtgtcaga aattttata atggagttg 
      gaacaatta ttaatgaca gtgctcctg accgagatt 
      gaaagtaac gaaagccaa caaaactcg ccatctgct 
      aataataat aatagtccg ataaattta tcaaatacg 
      tctccggta acacgcagt caggacgaa caagccgat 
      tcgaatgtt aggtcatca ccggtttca atgtcggcc 
      agtagggat caggatttg attcggcca aatgctgta 
      cctttggca ttcagtact tttcggaga ttgatccaa 
      agacaggag aatcaagag tcggaatcc agaactccc 
      acttcgaat tcagacgag ccttcgcgc tccagtccc 
      ggaagcagt aacgggatt cattcaagg acaagttcc 
      ccaatttat aacaggact tatcccagg cggaggctg 
      ccagcgcac cgcatttct gcgttcatg ccaacgcgg 
      gttaactat aatcgaatt ccgatgaga tacaggagg 
      ggctcctcc ttcagatta gcaaacaga ttatcggtc 
      aatagcacg ttcccgatc gacgaactc ataaactac 
      tctgagcgt ccgaacgcc gaagacaac gtcccgcaa 
      gacaacctc cccgaaagc agccctctt gagccccca 
      actgagcct tcccccttc gtcaacctg ggcaacatg 
      tactcaaac atcgtccaa gatctcgaa tctagctta 
      aatgacgtc agaaacatc cgttcgagc acacgtccg 
      agcgaaacc tccgacatg ttgagcagt ttttccgaa 
      agtctcgag aatataatg aaccaatcg gatacgatt 
      ttgcgaaac ttgggcggc tcaatgtca atgttaccg 
      aactattcc gaacaaaac gaaacacgc cgacgtgaa 
      acaaccgaa ccgagagcc actttcaac gaccagaat 
      ttctacatt cggagcaat tcgccacaa gaggcctcc 
      actagcacc ggagagaga aatctaagc gaggtgttg 
      gcaggtggc cgaagtgtg gtcgagtcc gatcacaac 
      tacccgaga aatccgggg aataaccaa gggcccagt 
      tccggtgaa aatatgtcg ccgttgatg acgtcccta 
      catttaacc atttcgtac atacagagg caagcacgg 
      cttttgaga caccaagtc gagagtatc gaacgcatc 
      gatcggacg atgtttgaa gtcgaacaa ttgcaatta 
      atcagacaa ttgatcacc gaatttttg cgctatgtc 
      agatctcta gccggtgag agtcgtagc acgggcatg 
      tcaagcgtg cgccaaatg atggccggg actagaatc 
      agcgattcg agtccttac gactcccag tcagaggag 
      cggccccaa cagcccccc caacccact cccagttcg 
      agcacctca accgatcaa agccagccg cgaccgagg 
      acttcaggc cgaaaaacc tacccacct tcgcgcctt 
      ttccgccta caacgccac aatcggcgc tccttattc 
      gtcaacatc ttcccacga agatacacg ggacggccc 
      gaacgcggc gccaaacac accggacct tgtcgcaac 
      gccccctct cgtacacct gaagcgaca aatccacta 
      cgacattta tcaagtcac ttattaatc aattcctcg 
      agtttgaat attatggcg cgccgactt gaacatttc 
      ctaaccgaa caaacccga ctcattacg cggccacaa 
      gagagtggc tctgggtca attcgctcg actgcagcg 
      accgaattg ggcgaaaga attttatcg attcggtta 
      tacgactgt gttatgcga atgaacagg attatgggt 
      tttgatagt agtcggttt cggaactcg aggagtgac 
      ctagttgtg gtgacacaa gatggggcg tccagattc 
      ccagctcgt cacttcctc agtctgatt gttgatgcg 
      atgagtaaa catttggag gagttggcg gggtcgaat 
      atatcgcaa agtttgcgc gggcagata catagtgta 
      ttggcaatg gcgctttta ctgtccgac cttttgctt 
      ctacagttg gttgattca atcccgcca cctgccggg 
      atgaactta gacgtggaa cgggaatct ttaacgtca 
      cgcatcgat caaatgtgc ggccaaatg ttgcaaaat 
      cgcttcagt ggacattcg catcaattg acacggagt 
      ttgcaacac atgagattg aatatgagg cgggcgtat 
      cgagctttg ggacaaact tacaacgct cggaggaac 
      gcaatgtta ccgaataga cccgatacg aaccgaaga 
      caattatta aatagatac ttgaggaat atcaatttg 
      agacgccac caaaattcc acatcaagc gacacaagc 
      gcgaatgtg gccccttct gcaccggcc gagtcagag 
      aatgtcact cgtggctgg agcagctta cgcgattta 
      ataatgcgt tattcggac aatacgggc gaggatgag 
      gacgtgaca agctctaac tcgaatcag gactctcaa 
      gagcgtacg caaagattt gagacagtt tctagtaac 
      aatagtgac gacaacgac gatgagtcg gagtggtac 
      gtcaataac aacaaccgc agtaccaac ctctaccgc 
      acaaacaat gttaacttg gtccataac aatccggaa 
      gagtcgaac ccttctcgg ccctggaac gttccctcc 
      gtgcaagtt aacgacgtt ccggtcccg attgcggaa 
      caaccttca ctgttccag cagcgaatt atgacgcac 
      cggcagaga ctggccgag agagtttca gagctgcgg 
      acgtttccg caaatgggc ggcttgagg ccgaggttt 
      ttgcacccg ctttacgcc agcgttaat ccgtttgat 
      gccgatctg gatgatcca caaagagag actaataat 
      tacgactgt gatatgata acgactgtt acgccgaat 
      catcggatc caaatgtgg gatatctcg tccggaaat 
      attccagtc atcaataat ccactgaaa aacctaatt 
      gtgagcgaa tgtaagatc cataacgac gctagtgtg 
      gatattgcg aaagacggg accatttta gtgacctta 
      ttgccatcg ggtggttac ttgaacgta acgaacaaa 
      cttggagtc tatagtttg aggtgggac acgttgggt 
      cagtgcttg tacactacc agttttgaa caaaacgcg 
      gtttcggtg gcgctgtcg ccgctaagt cggcatttg 
      gttgttggg ctggcgtct agaagagtg tccattgtt 
      cccagtgat aggtggacg atggcacgg atttttatt 
      atcgaacag aagaatgct cccggggat aggttgccc 
      gttttaagg gagttgggg caaaatagg gacagtagg 
      gccaattac aaaagcgtt aattgtatt aggtggctc 
      ccgacgtct ggccaagga ctcatttat gccacaaat 
      actggacag ttgggaaaa ggcaataaa cgtgttaac 
      gcaaacggt aaattcatc ccaatgatg cggccccgc 
      ggccccttt cctccaccc cgtggcccc atgggcatg 
      cggcccatg atggggccc ccgcatatg cgcccacct 
      ccacctcgt ggccttcgc ccaccaccg ccccgcctc 
      cccagaggg cccataatg ccgcctcgg atgatgggc 
      cccggccca cgacccctg ccaccgccc ggcatgcgg 
      cctccgccg ccacacatg atgggcccc atgcgcggg 
      atgggtttc agacgcggg atgccgcgc aagcccccc 
      atgggggcc ttcaagggg gtgcaaagg ggccgagtg 
      ttgaagcgg cgacgtccc atgatcaaa gacattgac 
      ttgacgaaa gagtgggtg acggacgcg attagacaa 
      gagtttaag aagaaggag gagctgttg gcggctgcg 
      aagagtgcg gagagcaag gaggcctgg gccacgtac 
      aggacgcag agggagaag tgcagtcaa atgtaccat 
      gtggcggag atggagttc gttgggctg caggagacg 
      accgaacaa gactgggac gacttcgaa tacgaagaa 
      aatccagag tctgaagtg tttttttgc gatgtttgc 
      gaagaggat ttttatacg cgagagcat tacgagaat 
      cacatgtcg cagcaccga atatgcggt gtggatggg 
      tgccagtac acagcgcct caaaaaatg atcgaaaaa 
      catatccaa atgcaacat ttgactggc ctttatgaa 
      aaaattcga agtgttaat acgcccgaa gaaatcgcg 
      aaatggatc gaagaacga aaagttcga tacccgagc 
      agggaaaat gtccaaaag aggtacgaa agacgggag 
      gagatgttg aaaaggggg gagaaaatt ggggagagg 
      aaaaatcga ttcgggaag gataagaca agactgtca 
      cgagtcagg cacaaaaaa caacagcgg aaaaatgtg 
      ccgaaagtt gtcaaaaat cccgaacaa gcgaagcaa 
      tctttaatt gatgaaaaa tgtgactgg aatgggaca 
      atgtttccg tttcgaggc accaaggaa ttatataac 
      gaagaggat gagaaggaa acgagtgat tacgatgat 
      gaggaatgg atggatggt tcgggaaaa gtcactgtt 
      gaattaaat aatgcattg ggagcacta atgggtgcg 
      tatgagtcg gaccaagaa gaagaaacg cctcagact 
      gtcgataat gacgctcca actgaagaa aaaatacaa 
      agagaacaa gtagaatat ccaaatgaa caagcgagt 
      gatactaaa gtcgtcaaa acaaacaag cggagaaga 
      ttatctagt ggtaagaaa caaaaacta aataacaaa 
      cctgaaccg ggtggcttt tcgcgaatg aaattcgtt 
      aaacgtaag gtaactctg ttggaaaag ttgttagat 
      agtgaaata aggcacgag cgcaatgtc ttactacaa 
      tgtgtacag catgtcgtc actaataat ttcttcaga 
      taa
    mRNA sequence
    • >TC033221 RA
      tccaaaaat cgttacaag acctgttaa ttactgaaa 
      taattggaa gaaaaacag tgcagtatc aagacatta 
      tgagaagat gaacccaga tgaatatga ctttcgtcg 
      aaatatccc caacaaaga gccctccag ccaggccct 
      agacttcat ccggggcct cgaagacag gagcctcaa 
      ccacctaaa gacaaacat cagagcaaa agccagccg 
      agaagtttt cgccgaaga tgaactggt cagaaaaaa 
      atgctacga cgaaatgcg ctgcgagat gccaggcca 
      acccagatc cacatttct catggtctt cagcccaga 
      tggaaccaa agttgcctc cacccacgg caaccacaa 
      catctacgt cacggatat caaaagcgg gaaaaacat 
      aaagactct gacaggaca cccgaggac tccgtggtg 
      cattgcctt ccacccaac ctccaacca aattgtggc 
      gtctggctg cctgggagg ggaagtcag gatttggga 
      tctgagtgg aggtagcga ggtctggac tgccgggaa 
      tcaatccgt aattgcgtc aattgcgtt ccatcccaa 
      tgaccggat tttggtcat tgcgacata caatgaact 
      gtacttttg ggactggag catgccgga gccgttcgt 
      ccaagtggc gactgccaa tcccaagga aaagattag 
      atatgtggc gtttgataa gttgggcca taaattgat 
      tacggggat tgcgaacag cccccagac gaggtggga 
      acgggtaag ggcccctgt ccctgtccc gagacaagc 
      agaaaggtc tgctagccc ctacagact tagaattac 
      gccaagact ggtcaacac accttcacc cccagagag 
      gacttattt taatgtagg cttacgtcc agagcaacc 
      tagcgttgt tgaaagcac tcgtgaggt actttccag 
      tgttcctga acgagaaag acgcataac ggcatctta 
      tcgtaacct tctaagaga gtacgaaca attggtcca 
      acgctattt gaaagttta caaacctcc aactatgat 
      tgaccgggg cactgaccc catggaacc caacgccac 
      gagtgccac cagcagcac tcaagcccc cgaatccga 
      accaagcac ttcgggaac gaacccaac tgaatctca 
      accttccac ctcccaaaa ctacagttc atcccttgt 
      catgccaag ccgtatttt agcaatgaa aaaacaacc 
      aactgaagc tcacagcac ccaaacttc agactcgag 
      aaaacataa atcgtccga aaatacaga ggaatcgac 
      tcaagaaaa acgaagtaa atcaagcga ttctggccc 
      aagtgggtc gtcacatac tgaaacagc gacacaaac 
      ccaaagtcc cagaagact gctagtgat aaatgaaag 
      gccccaacc cgaaccttc aacaagtgg gctgcagga 
      caaacctgt tagaaattc aggagtgcg gattttgtg 
      cccgaaaaa acgcaagtt accagaagc tgggggctc 
      gtctagcga gccccagcc gagcacaag tcgggggag 
      cacttcggg ggatattct gcaaaatat tcgccccat 
      tgccgaaga agtgcggaa tcggttctt gcccattat 
      caagtcgct ccccgcctg tgaccgacc gaaattaat 
      tcgacgttt tgagaacgg gcgggctca tgaaagact 
      gaagttgag acaaatgtg tccaacttt tttgaaaaa 
      gccgaatcg gaggccagt ccttgacac ctcttctga 
      tagtagtag ttctgacga cgaacgagg tggcacttt 
      acgttcacg aatgcccat agatctcac aacaaatac 
      aactaatca aaatagaaa tgcaaccaa tcaaaatga 
      gcctgaacc agaatgtgt tcataccgc cagcaatag 
      taaccctcc aagcaaccc ttcaccttc aaactcaaa 
      caattcaaa catcagtgg tgtcagaaa ttttataat 
      ggagttgga acaattatt aatgacagt gctcctgac 
      cgagattga aagtaacga aagccaaca aaactcgcc 
      atctgctaa taataataa tagtccgat aaatttatc 
      aaatacgtc tccggtaac acgcagtca ggacgaaca 
      agccgattc gaatgttag gtcatcacc ggtttcaat 
      gtcggccag tagggatca ggatttgat tcggccaaa 
      tgctgtacc tttggcatt cagtacttt tcggagatt 
      gatccaaag acaggagaa tcaagagtc ggaatccag 
      aactcccac ttcgaattc agacgagcc ttcgcgctc 
      cagtcccgg aagcagtaa cgggattca ttcaaggac 
      aagttcccc aatttataa caggactta tcccaggcg 
      gaggctgcc agcgcaccg catttctgc gttcatgcc 
      aacgcgggt taactataa tcgaattcc gatgagata 
      caggagggg ctcctcctt cagattagc aaacagatt 
      atcggtcaa tagcacgtt cccgatcga cgaactcat 
      aaactactc tgagcgtcc gaacgccga agacaacgt 
      cccgcaaga caacctccc cgaaagcag ccctcttga 
      gcccccaac tgagccttc ccccttcgt caacctggg 
      caacatgta ctcaaacat cgtccaaga tctcgaatc 
      tagcttaaa tgacgtcag aaacatccg ttcgagcac 
      acgtccgag cgaaacctc cgacatgtt gagcagttt 
      ttccgaaag tctcgagaa tataatgaa ccaatcgga 
      tacgatttt gcgaaactt gggcggctc aatgtcaat 
      gttaccgaa ctattccga acaaaacga aacacgccg 
      acgtgaaac aaccgaacc gagagccac tttcaacga 
      ccagaattt ctacattcg gagcaattc gccacaaga 
      ggcctccac tagcaccgg agagagaaa tctaagcga 
      ggtgttggc aggtggccg aagtgtggt cgagtccga 
      tcacaacta cccgagaaa tccggggaa taaccaagg 
      gcccagttc cggtgaaaa tatgtcgcc gttgatgac 
      gtccctaca tttaaccat ttcgtacat acagaggca 
      agcacggct tttgagaca ccaagtcga gagtatcga 
      acgcatcga tcggacgat gtttgaagt cgaacaatt 
      gcaattaat cagacaatt gatcaccga atttttgcg 
      ctatgtcag atctctagc cggtgagag tcgtagcac 
      gggcatgtc aagcgtgcg ccaaatgat ggccgggac 
      tagaatcag cgattcgag tccttacga ctcccagtc 
      agaggagcg gccccaaca gccccccca acccactcc 
      cagttcgag cacctcaac cgatcaaag ccagccgcg 
      accgaggac ttcaggccg aaaaaccta cccaccttc 
      gcgcctttt ccgcctaca acgccacaa tcggcgctc 
      cttattcgt caacatctt cccacgaag atacacggg 
      acggcccga acgcggcgc caaacacac cggaccttg 
      tcgcaacgc cccctctcg tacacctga agcgacaaa 
      tccactacg acatttatc aagtcactt attaatcaa 
      ttcctcgag tttgaatat tatggcgcg ccgacttga 
      acatttcct aaccgaaca aacccgact cattacgcg 
      gccacaaga gagtggctc tgggtcaat tcgctcgac 
      tgcagcgac cgaattggg cgaaagaat tttatcgat 
      tcggttata cgactgtgt tatgcgaat gaacaggat 
      tatgggttt tgatagtag tcggtttcg gaactcgag 
      gagtgacct agttgtggt gacacaaga tggggcgtc 
      cagattccc agctcgtca cttcctcag tctgattgt 
      tgatgcgat gagtaaaca tttggagga gttggcggg 
      gtcgaatat atcgcaaag tttgcgcgg gcagataca 
      tagtgtatt ggcaatggc gcttttact gtccgacct 
      tttgcttct acagttggt tgattcaat cccgccacc 
      tgccgggat gaacttaga cgtggaacg ggaatcttt 
      aacgtcacg catcgatca aatgtgcgg ccaaatgtt 
      gcaaaatcg cttcagtgg acattcgca tcaattgac 
      acggagttt gcaacacat gagattgaa tatgaggcg 
      ggcgtatcg agctttggg acaaactta caacgctcg 
      gaggaacgc aatgttacc gaatagacc cgatacgaa 
      ccgaagaca attattaaa tagatactt gaggaatat 
      caatttgag acgccacca aaattccac atcaagcga 
      cacaagcgc gaatgtggc cccttctgc accggccga 
      gtcagagaa tgtcactcg tggctggag cagcttacg 
      cgatttaat aatgcgtta ttcggacaa tacgggcga 
      ggatgagga cgtgacaag ctctaactc gaatcagga 
      ctctcaaga gcgtacgca aagatttga gacagtttc 
      tagtaacaa tagtgacga caacgacga tgagtcgga 
      gtggtacgt caataacaa caaccgcag taccaacct 
      ctaccgcac aaacaatgt taacttggt ccataacaa 
      tccggaaga gtcgaaccc ttctcggcc ctggaacgt 
      tccctccgt gcaagttaa cgacgttcc ggtcccgat 
      tgcggaaca accttcact gttccagca gcgaattat 
      gacgcaccg gcagagact ggccgagag agtttcaga 
      gctgcggac gtttccgca aatgggcgg cttgaggcc 
      gaggttttt gcacccgct ttacgccag cgttaatcc 
      gtttgatgc cgatctgga tgatccaca aagagagac 
      taataatta cgactgtga tatgataac gactgttac 
      gccgaatca tcggatcca aatgtggga tatctcgtc 
      cggaaatat tccagtcat caataatcc actgaaaaa 
      cctaattgt gagcgaatg taagatcca taacgacgc 
      tagtgtgga tattgcgaa agacgggac cattttagt 
      gaccttatt gccatcggg tggttactt gaacgtaac 
      gaacaaact tggagtcta tagtttgag gtgggacac 
      gttgggtca gtgcttgta cactaccag ttttgaaca 
      aaacgcggt ttcggtggc gctgtcgcc gctaagtcg 
      gcatttggt tgttgggct ggcgtctag aagagtgtc 
      cattgttcc cagtgatag gtggacgat ggcacggat 
      ttttattat cgaacagaa gaatgctcc cggggatag 
      gttgcccgt tttaaggga gttggggca aaataggga 
      cagtagggc caattacaa aagcgttaa ttgtattag 
      gtggctccc gacgtctgg ccaaggact catttatgc 
      cacaaatac tggacagtt gggaaaagg caataaacg 
      tgttaacgc aaacggtaa attcatccc aatgatgcg 
      gccccgcgg cccctttcc tccaccccg tggccccat 
      gggcatgcg gcccatgat ggggccccc gcatatgcg 
      cccacctcc acctcgtgg ccttcgccc accaccgcc 
      ccgcctccc cagagggcc cataatgcc gcctcggat 
      gatgggccc cggcccacg acccctgcc accgcccgg 
      catgcggcc tccgccgcc acacatgat gggccccat 
      gcgcgggat gggtttcag acgcgggat gccgcgcaa 
      gccccccat gggggcctt caagggggt gcaaagggg 
      ccgagtgtt gaagcggcg acgtcccat gatcaaaga 
      cattgactt gacgaaaga gtgggtgac ggacgcgat 
      tagacaaga gtttaagaa gaaggagga gctgttggc 
      ggctgcgaa gagtgcgga gagcaagga ggcctgggc 
      cacgtacag gacgcagag ggagaagtg cagtcaaat 
      gtaccatgt ggcggagat ggagttcgt tgggctgca 
      ggagacgac cgaacaaga ctgggacga cttcgaata 
      cgaagaaaa tccagagtc tgaagtgtt tttttgcga 
      tgtttgcga agaggattt ttatacgcg agagcatta 
      cgagaatca catgtcgca gcaccgaat atgcggtgt 
      ggatgggtg ccagtacac agcgcctca aaaaatgat 
      cgaaaaaca tatccaaat gcaacattt gactggcct 
      ttatgaaaa aattcgaag tgttaatac gcccgaaga 
      aatcgcgaa atggatcga agaacgaaa agttcgata 
      cccgagcag ggaaaatgt ccaaaagag gtacgaaag 
      acgggagga gatgttgaa aagggggga gaaaattgg 
      ggagaggaa aaatcgatt cgggaagga taagacaag 
      actgtcacg agtcaggca caaaaaaca acagcggaa 
      aaatgtgcc gaaagttgt caaaaatcc cgaacaagc 
      gaagcaatc tttaattga tgaaaaatg tgactggaa 
      tgggacaat gtttccgtt tcgaggcac caaggaatt 
      atataacga agaggatga gaaggaaac gagtgatta 
      cgatgatga ggaatggat ggatggttc gggaaaagt 
      cactgttga attaaataa tgcattggg agcactaat 
      gggtgcgta tgagtcgga ccaagaaga agaaacgcc 
      tcagactgt cgataatga cgctccaac tgaagaaaa 
      aatacaaag agaacaagt agaatatcc aaatgaaca 
      agcgagtga tactaaagt cgtcaaaac aaacaagcg 
      gagaagatt atctagtgg taagaaaca aaaactaaa 
      taacaaacc tgaaccggg tggcttttc gcgaatgaa 
      attcgttaa acgtaaggt aactctgtt ggaaaagtt 
      gttagatag tgaaataag gcacgagcg caatgtctt 
      actacaatg tgtacagca tgtcgtcac taataattt 
      cttcagata aaactgtga tcattttta cgcaatata 
      tttttgtaa agtat
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