Status
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Gene information
General information
TC013188 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12591808..12595305
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC013188
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_07338

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC013188 RA
        MSHYSRNNN SFVTPPRAS FRRYSLADT SSLTPTRAT 
        SALYNNHLS TKIGSRSPG YTTRVVQYS EEHGRSRPS 
        HQHKYNKLG SFPIVHFFK KELPSLKRK GSEGNISQM 
        NSAELSRFN SPEQNRLLL EKIIKSSPN NDSISHKTE 
        TNTKSVLDA LKEISRKRI HASEEDFIL KDEGKRLHK 
        EYDNEGASR KRLRQNSLS PSSRYSSIS ELIASHSSM 
        DGYLEEEKK KENLVKLSV PPVTPLKTP PIVKTCVDV 
        ETQTIIPEP TPDSSKVVI PQKEEKENV SVNEARPQV 
        KVKKASPLK IFDETPLET MRKNRLTAL MGSVQNKNQ 
        KQEEETKSD EKSAKPLVS ILSPTNKSP HKSNKHVTF 
        DIPQSENSP VSSTTPVTF SESTTPTKL VEAVTSTST 
        AISTPIVSA SVVEVPPMT FKAAGDAPP PAVTSTPPA 
        SKTISFGNT NTFSVSSTV TPTTFTSLP TVKPTEVKP 
        TVEKTPLPS FSSPSQNFE PKTSVTTSN FTFGKLSEP 
        VVPTQKGGF KFELKDATT SVGTSSPFQ FKSPAVTTS 
        APAEKPVST FGTIKPANS LQLAQVTSS NPVTTLPTF 
        GVTTTSSDS PSKNFLMPS ATMFSVNKT SSPTTSTFM 
        RPPNPLQTA NSSMFNPPP SSNKPSMFS FGSTSTATS 
        TATFAPSFT PSTTPGLFE PKSALSGSA KPSLNFSAP 
        NSFTMSPVT TSSSFALPP VTTTSAMPF AFKPTTSFS 
        STVASTTST FSNPVFGTN TTANFGGGA SGAKPVFGQ 
        QAATGPLFS TTGSSFGTT NTFGTTSAF GANNSSQNV 
        FGAVATTTS SIFGGSNTF GTAGSSFGN SFGSGQSSA 
        FNEPKSQPT PSFGTPGSS AFGVTTTAS FGTNSTVFT 
        TTASGFSAS NNTFSSTFG SSSAFDKPA EAASPFGGG 
        GSTFGGATF GANPPVTQS GVFAFGTEG TKPPVFSFG 
        SAEPPKPSF NFTAGSGGF DAGKGFGGN PPPAFNPGL 
        TPQFNMPAP VGTGMFNIG SGGTSTPNR SRTMHKTKR 
        RT
      CDS sequence
      • >TC013188 RA
        atgtcacac tattcaagg aacaacaat tcgtttgtg 
        actccacct cgggcatcc tttcgacgg tattcgtta 
        gcggacaca tcgtctctg acaccaaca agagcgaca 
        agcgcgctg tacaataat cacttgagc accaaaatt 
        ggcagtagg agccctggt tatacaact cgagtggtg 
        caatacagc gaggagcat gggcgatcc aggcctagc 
        caccagcac aagtataac aaactgggc agcttccct 
        attgttcat ttcttcaag aaagaatta ccaagttta 
        aaaagaaaa ggtagtgaa ggcaacatt tcccaaatg 
        aattcggct gagttgtca aggtttaat agtcccgaa 
        cagaacaga ttattgttg gaaaaaatt atcaaaagt 
        agtccgaac aatgacagc atttcgcat aaaactgag 
        accaacacc aagagcgtt ttagacgcc ttgaaggaa 
        atttcgcgg aaacggatt cacgccagt gaggaggac 
        tttatcttg aaagatgag ggtaaaagg ttgcacaag 
        gagtatgat aacgagggg gcttcaaga aaacgctta 
        agacaaaat tccttgagt ccttcaagt cgttattcg 
        tcgattagt gaactaatc gcgtcacat tcgtcaatg 
        gatggatat ttagaagaa gagaagaag aaggagaat 
        ttagtaaaa ttgtcagtt ccaccagtc acccccttg 
        aagacccct ccaatcgtc aagacgtgt gttgacgtt 
        gaaactcaa actattatc cctgaaccc accccagac 
        tccagtaaa gtagttata ccacaaaag gaagaaaag 
        gaaaatgtg agtgttaac gaagctcga ccacaagtc 
        aaggtgaaa aaagcgtcc cctcttaaa attttcgat 
        gaaacacct ctggaaaca atgcggaag aatcgactc 
        acagctttg atgggttca gttcaaaat aagaatcaa 
        aaacaagaa gaagagacg aaaagtgac gagaaatca 
        gcaaaacca ctcgtctcc atcctctca ccgactaac 
        aaaagcccc cacaagagt aacaaacat gttactttt 
        gatatcccg cagtcggaa aattctccc gtttcaagt 
        actaccccg gtcacgttt agtgaaagt accactcca 
        actaaactt gtagaagct gtaactagt acttccaca 
        gctataagt actccgatt gtttcggca agtgttgta 
        gaggtgcct cctatgact tttaaggcg gcaggtgat 
        gcgcctcca cctgccgtt acctcaact cccccagct 
        agtaaaacc ataagtttt ggaaacaca aacacattc 
        agtgtttcc agtactgtt actccaacc acgtttact 
        agtttacca accgttaaa ccaactgaa gtgaagccc 
        acagttgag aaaacacct ctaccaagc ttttccagt 
        ccgagtcaa aattttgaa ccaaaaact agtgtgaca 
        actagtaat tttacgttt gggaaactc tctgaacca 
        gttgtgcca acacagaaa ggcgggttc aagtttgag 
        ctaaaggac gctactact agtgttggt acatcgtca 
        ccgtttcag tttaaaagt cccgcagtg accacgtcc 
        gctcccgcg gaaaaacca gttagtact ttcggtact 
        attaaacca gcaaattcg ttacaactt gcacaagtc 
        acttcttcc aacccagtg actactttg cctacgttt 
        ggtgtaaca acaacttcg agtgattcc cccagcaag 
        aatttccta atgccgtct gctaccatg ttttccgta 
        aacaaaact agttctcca accacaagt acgttcatg 
        cgcccgccg aatccgctc caaacagct aactcgtca 
        atgtttaat cctccacca tcctccaac aaaccgtca 
        atgttttcg ttcggtagt acaagcact gcaacttca 
        accgcaact tttgctcca agttttacg ccctcgaca 
        actcccggc ctgtttgag cccaaatct gccttaagt 
        ggttccgcg aaaccgtcg ttgaatttt tccgccccc 
        aattcattc acaatgtct ccagtgacc acttcaagt 
        agctttgcc ctgcctcct gtaacgaca acttccgca 
        atgcccttc gctttcaag ccgactact agttttagt 
        agtactgtg gcatcgacc acttcaaca ttttcgaac 
        ccagttttt ggtactaac actactgcg aattttggt 
        ggtggtgct agtggagcg aaaccagtt tttgggcaa 
        caagcagcg actggccct ttgttttca accactggt 
        tccagtttt ggtaccact aacactttt ggcacaact 
        tcagctttt ggagcaaac aactccagc cagaacgtc 
        ttcggggct gttgccaca acaacgagt agtattttt 
        ggcggttct aatactttc ggtacggct ggttcttcc 
        ttcggtaac tcgttcgga agtgggcaa agctccgca 
        tttaacgaa cccaaaagc caaccaaca ccttcattt 
        ggtactccc ggaagttcg gctttcggt gtcacgact 
        accgcaagt ttcggtacg aattcaacg gtttttacc 
        acaacagcg tccggtttt tcggcaagt aataatact 
        ttttcctcg acttttggc tcaagttca gcgtttgat 
        aagccagca gaagcggca agtccgttt ggtggtggt 
        ggtagtacc tttggaggt gctactttt ggtgccaat 
        cctccagtt acacaaagt ggtgttttt gcgtttggt 
        actgaaggt acgaaaccg cctgttttt tcgttcggt 
        tccgcagaa ccgcccaaa ccttcgttt aattttacg 
        gcaggttcg ggtggtttc gatgctggg aaaggtttc 
        ggtgggaat ccaccccca gcgtttaat cccggtttg 
        acgccgcag ttcaatatg ccagctcct gtgggaacg 
        gggatgttt aatataggc agtgggggg acgtccacg 
        cccaatagg tccaggact atgcataag actaagcga 
        agaacgtga
      mRNA sequence
      • >TC013188 RA
        aacgcatca tttgacgga tttgacatt ggcgggcgt 
        caaaccggt acccgctaa cgtcaaatt tctaattta 
        ccattttta caaaaaaat tgttttcct gggaaggtg 
        taagtctgt gcgttgtgt agtcgtggt cactttttc 
        tcgctacaa tattgcaca cattgcttg agaattgtg 
        taacaaaat tacggcctt attgcgctt gtagtggag 
        gttatgtga acaggactt cacagaaat gcgactaca 
        actttaatc gaacgtgtt aaaccgtga ttaactttt 
        gagagacac acaatgtca cactattca aggaacaac 
        aattcgttt gtgactcca cctcgggca tcctttcga 
        cggtattcg ttagcggac acatcgtct ctgacacca 
        acaagagcg acaagcgcg ctgtacaat aatcacttg 
        agcaccaaa attggcagt aggagccct ggttataca 
        actcgagtg gtgcaatac agcgaggag catgggcga 
        tccaggcct agccaccag cacaagtat aacaaactg 
        ggcagcttc cctattgtt catttcttc aagaaagaa 
        ttaccaagt ttaaaaaga aaaggtagt gaaggcaac 
        atttcccaa atgaattcg gctgagttg tcaaggttt 
        aatagtccc gaacagaac agattattg ttggaaaaa 
        attatcaaa agtagtccg aacaatgac agcatttcg 
        cataaaact gagaccaac accaagagc gttttagac 
        gccttgaag gaaatttcg cggaaacgg attcacgcc 
        agtgaggag gactttatc ttgaaagat gagggtaaa 
        aggttgcac aaggagtat gataacgag ggggcttca 
        agaaaacgc ttaagacaa aattccttg agtccttca 
        agtcgttat tcgtcgatt agtgaacta atcgcgtca 
        cattcgtca atggatgga tatttagaa gaagagaag 
        aagaaggag aatttagta aaattgtca gttccacca 
        gtcaccccc ttgaagacc cctccaatc gtcaagacg 
        tgtgttgac gttgaaact caaactatt atccctgaa 
        cccacccca gactccagt aaagtagtt ataccacaa 
        aaggaagaa aaggaaaat gtgagtgtt aacgaagct 
        cgaccacaa gtcaaggtg aaaaaagcg tcccctctt 
        aaaattttc gatgaaaca cctctggaa acaatgcgg 
        aagaatcga ctcacagct ttgatgggt tcagttcaa 
        aataagaat caaaaacaa gaagaagag acgaaaagt 
        gacgagaaa tcagcaaaa ccactcgtc tccatcctc 
        tcaccgact aacaaaagc ccccacaag agtaacaaa 
        catgttact tttgatatc ccgcagtcg gaaaattct 
        cccgtttca agtactacc ccggtcacg tttagtgaa 
        agtaccact ccaactaaa cttgtagaa gctgtaact 
        agtacttcc acagctata agtactccg attgtttcg 
        gcaagtgtt gtagaggtg cctcctatg acttttaag 
        gcggcaggt gatgcgcct ccacctgcc gttacctca 
        actccccca gctagtaaa accataagt tttggaaac 
        acaaacaca ttcagtgtt tccagtact gttactcca 
        accacgttt actagttta ccaaccgtt aaaccaact 
        gaagtgaag cccacagtt gagaaaaca cctctacca 
        agcttttcc agtccgagt caaaatttt gaaccaaaa 
        actagtgtg acaactagt aattttacg tttgggaaa 
        ctctctgaa ccagttgtg ccaacacag aaaggcggg 
        ttcaagttt gagctaaag gacgctact actagtgtt 
        ggtacatcg tcaccgttt cagtttaaa agtcccgca 
        gtgaccacg tccgctccc gcggaaaaa ccagttagt 
        actttcggt actattaaa ccagcaaat tcgttacaa 
        cttgcacaa gtcacttct tccaaccca gtgactact 
        ttgcctacg tttggtgta acaacaact tcgagtgat 
        tcccccagc aagaatttc ctaatgccg tctgctacc 
        atgttttcc gtaaacaaa actagttct ccaaccaca 
        agtacgttc atgcgcccg ccgaatccg ctccaaaca 
        gctaactcg tcaatgttt aatcctcca ccatcctcc 
        aacaaaccg tcaatgttt tcgttcggt agtacaagc 
        actgcaact tcaaccgca acttttgct ccaagtttt 
        acgccctcg acaactccc ggcctgttt gagcccaaa 
        tctgcctta agtggttcc gcgaaaccg tcgttgaat 
        ttttccgcc cccaattca ttcacaatg tctccagtg 
        accacttca agtagcttt gccctgcct cctgtaacg 
        acaacttcc gcaatgccc ttcgctttc aagccgact 
        actagtttt agtagtact gtggcatcg accacttca 
        acattttcg aacccagtt tttggtact aacactact 
        gcgaatttt ggtggtggt gctagtgga gcgaaacca 
        gtttttggg caacaagca gcgactggc cctttgttt 
        tcaaccact ggttccagt tttggtacc actaacact 
        tttggcaca acttcagct tttggagca aacaactcc 
        agccagaac gtcttcggg gctgttgcc acaacaacg 
        agtagtatt tttggcggt tctaatact ttcggtacg 
        gctggttct tccttcggt aactcgttc ggaagtggg 
        caaagctcc gcatttaac gaacccaaa agccaacca 
        acaccttca tttggtact cccggaagt tcggctttc 
        ggtgtcacg actaccgca agtttcggt acgaattca 
        acggttttt accacaaca gcgtccggt ttttcggca 
        agtaataat actttttcc tcgactttt ggctcaagt 
        tcagcgttt gataagcca gcagaagcg gcaagtccg 
        tttggtggt ggtggtagt acctttgga ggtgctact 
        tttggtgcc aatcctcca gttacacaa agtggtgtt 
        tttgcgttt ggtactgaa ggtacgaaa ccgcctgtt 
        ttttcgttc ggttccgca gaaccgccc aaaccttcg 
        tttaatttt acggcaggt tcgggtggt ttcgatgct 
        gggaaaggt ttcggtggg aatccaccc ccagcgttt 
        aatcccggt ttgacgccg cagttcaat atgccagct 
        cctgtggga acggggatg tttaatata ggcagtggg 
        gggacgtcc acgcccaat aggtccagg actatgcat 
        aagactaag cgaagaacg tgaatgttt tagtaaaat 
        taaat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY