Status
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Gene information
General information
TC007512 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3476454..3479158
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007512
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04340

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_07337

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007512 RA
      MTEKYTLIE HPSTLNSII RNTFQSTHR IKFTAFDVS 
      NKYVIFGVN SGGIYIFSR ESCQFVKLI PSTHGPANL 
      LSISPNEKN LAIAVSTGL VVVLEGFTE TEFQQQIFT 
      EHEDNHITA MTWNGNDLY CGDNTGRVS VVALRNFLT 
      MFQTPVACL MHLDSQIVQ LDTYSKFLL VSTQTRSLL 
      CDTESEKYR QIGKKMRDG CFGACFLTA GNPHKNNDS 
      NARTRGLFE ISGDSEQLS APSHDDKVK IYCARPGVR 
      LWQADFEAN VVVTHQFRP ALGLSHSKV VQWDDSDRL 
      KLKTLSPQT GEFNFGKIY NFLHRFVLT FRDDGFYIF 
      EPSSSTLHW SAHTSVKDV KIVDSFLYV WHSDLEVSV 
      LSLLSIEEL VLRSLMRKK YKLCAEICL EFYDDVQSL 
      IETSKKIHL ISILETKLD DSEVLLKLE PIFTRLKDC 
      AVTPTLERY KNGIVTVNA LPVLPESVE NFEKDTKKQ 
      ENSILTEQF RVSRFNKNI ETNDFTQLM KTLNSNELY 
      DVLREFESQ NESCADWCK EIFLKHLKI ISEFKSEAL 
      DYAHQAFFS FNMNMNFIC NCGFPLPQA HELRPKFYE 
      IGTQLMEKT DNYEDYLKS VPYMYKNVL EKINDSQEI 
      HSKLPLIIQ FSDPEIIDT HSSKLTYDL WDESIRCLL 
      KLKSGQCLN CGAKIDVRA TFDWTDFAT KIVDSIKAN 
      NALKLFKRY AHLLPKGGL KPVFYQYCI FSSASNTTS 
      GIFRKTLDN ENVCKQFET SVGGYLRKK YLCGSDLYD 
      PPNRKSMLD LINCDHCQL PLKIPMLEE TTKLTCGHN 
      YHQTCLLYN KSQCPKCDP LL
    CDS sequence
    • >TC007512 RA
      atgaccgaa aagtacact ttaatcgaa catcccagt 
      actttaaac agcataatc cgcaacaca tttcaatcg 
      acacaccga ataaaattc acagcgttt gatgtttcc 
      aacaaatac gtcattttc ggcgttaat agcggtggt 
      atttacatt ttcagtcgt gaatcttgc cagttcgtc 
      aaattaatc cctagtacc catggcccc gcaaactta 
      ctttctatt tccccaaat gagaaaaac ctcgcgatt 
      gctgtctca acagggctt gtggtcgtc ctcgagggc 
      tttaccgag acagaattc caacaacaa attttcact 
      gaacatgaa gacaaccac ataactgcc atgacatgg 
      aacggaaac gatctatac tgcggtgac aacactggc 
      cgggtttct gtcgtagcc ctccgaaat tttttgaca 
      atgtttcaa actccggtt gcttgtctc atgcactta 
      gactctcag atcgtgcaa ttggacacc tactctaag 
      tttctccta gtctccacg caaacccgt tcgttgtta 
      tgtgacacc gagtccgaa aagtaccga caaatcggt 
      aaaaaaatg cgcgatggt tgttttggc gcctgtttc 
      ctcactgcg gggaatccc cacaaaaac aacgacagc 
      aatgcacgt accagaggc ctttttgaa atttcaggc 
      gactcagaa caactatca gccccctca cacgacgat 
      aaagtcaaa atttattgc gctaggccg ggggtgagg 
      ctctggcag gctgatttt gaagccaat gttgtcgta 
      actcaccag tttaggccg gctttgggg ctaagtcac 
      agcaaagtt gtacaatgg gacgactca gaccgactc 
      aaattgaaa actctctcc cctcaaacc ggggaattt 
      aatttcggg aaaatttac aattttttg caccgattc 
      gttttgact tttcgggac gatggtttt tacattttc 
      gagccgtcc tcgagcact ttacactgg agtgcccac 
      acgagtgta aaagacgtg aaaattgtt gattcgttc 
      ttgtatgtt tggcatagc gatttggaa gttagcgtt 
      ttgtcgttg ttatccatt gaggaattg gttttgagg 
      agtttaatg aggaaaaag tataaattg tgtgcggaa 
      atttgcttg gagttttac gacgacgtc caatcgtta 
      attgaaacg tcgaaaaag attcactta atttcgatt 
      ttagaaaca aagttagat gactcagaa gtgttactt 
      aagttagaa ccgattttc acacgcttg aaagactgt 
      gcggtaaca ccgactcta gagagatat aaaaacgga 
      attgttaca gtcaatgcc ttaccggtt ttaccagag 
      tcggttgaa aattttgaa aaagacacc aaaaaacaa 
      gaaaacagt attttgaca gagcaattt agggttagt 
      aggtttaat aagaatata gaaacgaac gattttact 
      caattaatg aagacttta aattcgaac gaactttac 
      gacgttttg cgagaattc gagtcacaa aacgaaagt 
      tgtgccgat tggtgcaag gaaatattt ttgaaacat 
      ttaaaaatc atctctgag tttaaatca gaggcttta 
      gattacgca caccaggcc tttttctcc tttaacatg 
      aacatgaat tttatttgt aattgtggt tttccgcta 
      cctcaagcg cacgaattg cgcccaaag ttttacgaa 
      attgggact caactaatg gaaaagacc gataattac 
      gaagattat ctcaaaagt gtcccatac atgtacaaa 
      aacgttttg gaaaaaatc aacgattcg caagaaatt 
      cattccaaa ctgcccctc ataattcag tttagtgac 
      cccgaaata attgatact cactcgagt aaactgact 
      tatgatctt tgggacgag tcaattcgt tgtttactc 
      aaattaaag tccggtcag tgccttaat tgtggtgct 
      aaaatcgac gttagggcc acttttgac tggactgat 
      tttgcaaca aaaatagtc gattcgata aaggcaaac 
      aacgccctt aagctgttc aaacgttac gctcattta 
      ttacctaag gggggatta aagccggtt ttttatcag 
      tattgcatt ttttcgtcc gcttcaaat acaacaagc 
      ggcattttt aggaagact ttagataac gaaaatgtc 
      tgtaagcag tttgagacg tccgtagga ggatatcta 
      cgtaagaaa tatttgtgt ggtagcgat ttatacgac 
      ccacccaat cgcaaatca atgctggat ttaataaat 
      tgtgatcac tgccaattg cctttaaaa attccaatg 
      cttgaagaa actacgaaa ctgacctgt ggacataac 
      taccatcaa acgtgtctt ttatacaac aagagtcag 
      tgtcctaaa tgtgatcca cttttgtag
    mRNA sequence
    • >TC007512 RA
      tgacttttc tctagttct attaaacct gcctgaata 
      gtgcaataa tgaccgaaa agtacactt taatcgaac 
      atcccagta ctttaaaca gcataatcc gcaacacat 
      ttcaatcga cacaccgaa taaaattca cagcgtttg 
      atgtttcca acaaatacg tcattttcg gcgttaata 
      gcggtggta tttacattt tcagtcgtg aatcttgcc 
      agttcgtca aattaatcc ctagtaccc atggccccg 
      caaacttac tttctattt ccccaaatg agaaaaacc 
      tcgcgattg ctgtctcaa cagggcttg tggtcgtcc 
      tcgagggct ttaccgaga cagaattcc aacaacaaa 
      ttttcactg aacatgaag acaaccaca taactgcca 
      tgacatgga acggaaacg atctatact gcggtgaca 
      acactggcc gggtttctg tcgtagccc tccgaaatt 
      ttttgacaa tgtttcaaa ctccggttg cttgtctca 
      tgcacttag actctcaga tcgtgcaat tggacacct 
      actctaagt ttctcctag tctccacgc aaacccgtt 
      cgttgttat gtgacaccg agtccgaaa agtaccgac 
      aaatcggta aaaaaatgc gcgatggtt gttttggcg 
      cctgtttcc tcactgcgg ggaatcccc acaaaaaca 
      acgacagca atgcacgta ccagaggcc tttttgaaa 
      tttcaggcg actcagaac aactatcag ccccctcac 
      acgacgata aagtcaaaa tttattgcg ctaggccgg 
      gggtgaggc tctggcagg ctgattttg aagccaatg 
      ttgtcgtaa ctcaccagt ttaggccgg ctttggggc 
      taagtcaca gcaaagttg tacaatggg acgactcag 
      accgactca aattgaaaa ctctctccc ctcaaaccg 
      gggaattta atttcggga aaatttaca attttttgc 
      accgattcg ttttgactt ttcgggacg atggttttt 
      acattttcg agccgtcct cgagcactt tacactgga 
      gtgcccaca cgagtgtaa aagacgtga aaattgttg 
      attcgttct tgtatgttt ggcatagcg atttggaag 
      ttagcgttt tgtcgttgt tatccattg aggaattgg 
      ttttgagga gtttaatga ggaaaaagt ataaattgt 
      gtgcggaaa tttgcttgg agttttacg acgacgtcc 
      aatcgttaa ttgaaacgt cgaaaaaga ttcacttaa 
      tttcgattt tagaaacaa agttagatg actcagaag 
      tgttactta agttagaac cgattttca cacgcttga 
      aagactgtg cggtaacac cgactctag agagatata 
      aaaacggaa ttgttacag tcaatgcct taccggttt 
      taccagagt cggttgaaa attttgaaa aagacacca 
      aaaaacaag aaaacagta ttttgacag agcaattta 
      gggttagta ggtttaata agaatatag aaacgaacg 
      attttactc aattaatga agactttaa attcgaacg 
      aactttacg acgttttgc gagaattcg agtcacaaa 
      acgaaagtt gtgccgatt ggtgcaagg aaatatttt 
      tgaaacatt taaaaatca tctctgagt ttaaatcag 
      aggctttag attacgcac accaggcct ttttctcct 
      ttaacatga acatgaatt ttatttgta attgtggtt 
      ttccgctac ctcaagcgc acgaattgc gcccaaagt 
      tttacgaaa ttgggactc aactaatgg aaaagaccg 
      ataattacg aagattatc tcaaaagtg tcccataca 
      tgtacaaaa acgttttgg aaaaaatca acgattcgc 
      aagaaattc attccaaac tgcccctca taattcagt 
      ttagtgacc ccgaaataa ttgatactc actcgagta 
      aactgactt atgatcttt gggacgagt caattcgtt 
      gtttactca aattaaagt ccggtcagt gccttaatt 
      gtggtgcta aaatcgacg ttagggcca cttttgact 
      ggactgatt ttgcaacaa aaatagtcg attcgataa 
      aggcaaaca acgccctta agctgttca aacgttacg 
      ctcatttat tacctaagg ggggattaa agccggttt 
      tttatcagt attgcattt tttcgtccg cttcaaata 
      caacaagcg gcattttta ggaagactt tagataacg 
      aaaatgtct gtaagcagt ttgagacgt ccgtaggag 
      gatatctac gtaagaaat atttgtgtg gtagcgatt 
      tatacgacc cacccaatc gcaaatcaa tgctggatt 
      taataaatt gtgatcact gccaattgc ctttaaaaa 
      ttccaatgc ttgaagaaa ctacgaaac tgacctgtg 
      gacataact accatcaaa cgtgtcttt tatacaaca 
      agagtcagt gtcctaaat gtgatccac ttttgtagt 
      ttttacagc ctaataaat tttaaaaac agga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY