Status
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Gene information
General information
TC003419 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4802071..4804795
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003419
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03554

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_07334

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003419 RA
      MSSPAPKSP EQSEKSKKY DRQLRLWGD HGQKFLENS 
      KICLINATA LGTEILKSL VLPGIGSFT IVDGEKVTD 
      DDIGSNFFI ESDSIGMSR AQVATQNLL ELNPDVRGD 
      YIDESVDHI MAHSQDFFD TFSVVIATC LPEKVLMPL 
      SRHLWEKNV PLIVCRSVG FLGYIRIQV KEHTIIEAH 
      PDNENHDLR LDNPWPALK EHLDKVDVT KLDNKERSH 
      VPAVVILYY YLAQFKSKH GHLPKTRAE KEEVKKMIT 
      ESPPPDEHG IKNLEENFK EAIRYINTC INPIKIPPN 
      LQAILEDDS CINVNQNSS PFWVLCAAL KEFVEKEGA 
      PPLKGTLPD MAADTSSYI TLQQLYQKQ AQTQCEIIY 
      RRALEIARN LGLSQETIT ESEAKLFCK HASELHVIR 
      GSCIADEYQ KTRVDLTSC LEDPDSLMF HYITLRGLE 
      RFISEFNSY PGQLDDHVE PDVLKLKGI IGKLLGEWG 
      CSQIIRDER VHEVCRYGG AELHSVSAI LGGCAAHEA 
      IKLITLQYK PLNNTFIYD AITSTSASF TL
    CDS sequence
    • >TC003419 RA
      atgtcatct cccgcgcca aagtcgccg gaacaaagc 
      gaaaagagc aaaaaatac gaccggcag ttgagatta 
      tggggcgat cacggtcag aaatttctc gaaaactca 
      aagatttgt ctaattaac gccaccgct ttgggtacg 
      gaaattctc aaaagtttg gttttgcct ggcattggt 
      tccttcacg attgttgat ggtgaaaaa gtaactgat 
      gatgatatt ggttcaaac ttttttatc gagagtgat 
      tcaataggg atgtctaga gcacaggta gccactcaa 
      aacctactt gagctgaac cctgatgtt agaggggac 
      tacattgac gagtccgtt gaccacatt atggcccac 
      agtcaagat ttttttgac acgttttct gtcgtaatt 
      gcaacgtgt ttgccagaa aaagtttta atgccgctc 
      tctagacac ttatgggaa aaaaatgta ccacttatt 
      gtatgccgc agtgtgggc tttctgggc tacataaga 
      atacaagtc aaagaacac accataata gaagcccac 
      cctgataac gaaaatcat gatttacgc ttagataat 
      ccatggcct gcacttaaa gaacatttg gataaagtt 
      gatgtaacc aaactagac aacaaggaa cgcagtcat 
      gtgcctgcg gtggttatt ctgtactat tatttggcg 
      cagtttaaa agcaaacac ggccattta ccgaaaact 
      cgagccgaa aaggaagaa gttaaaaaa atgataacg 
      gaaagcccc ccgcctgat gaacacgga attaagaac 
      ttggaggag aattttaaa gaggctatt cgctacatt 
      aatacttgt attaacccc atcaagatt cccccaaat 
      ttgcaagca attctggag gacgatagc tgcataaat 
      gtgaaccag aacagttca cctttttgg gttttatgt 
      gcggccttg aaggaattt gttgaaaaa gaaggggcg 
      ccccctttg aaagggact ttaccagat atggcagct 
      gacaccagt agctacatc acgttgcaa caactctac 
      caaaaacag gctcaaacc cagtgtgaa attatctac 
      aggagggcg ttagaaatt gcgcgaaat ttgggcctc 
      tctcaagag accataacc gaatctgaa gctaagcta 
      ttttgcaag catgcgagt gagttacat gtgattcgt 
      ggcagttgc attgctgat gaatatcaa aaaactaga 
      gtagatcta acgtcatgt cttgaggat cctgatagt 
      ttgatgttc cattacata actctaaga gggctagag 
      cgctttatc agtgaattt aatagttat ccagggcaa 
      ttggatgac catgtggag cctgatgtg cttaaattg 
      aagggcatt attgggaag ttgttagga gagtggggt 
      tgttcacag ataatcaga gatgagagg gtacacgaa 
      gtttgtcgc tacgggggc gctgaactg cactcagtc 
      tcggctatt ttgggcgga tgtgcggct catgaagcc 
      atcaaactt ataactctg cagtataag ccactaaac 
      aacaccttt atttacgat gcaattacc tcaacttct 
      gcctcgttt actctttaa
    mRNA sequence
    • >TC003419 RA
      gagtgaaaa aggccgaca gttttttaa attttacaa 
      gaagttatt gttgtgtaa cacataaaa agccttgtt 
      ttaagtttt taatcgata atgtcatct cccgcgcca 
      aagtcgccg gaacaaagc gaaaagagc aaaaaatac 
      gaccggcag ttgagatta tggggcgat cacggtcag 
      aaatttctc gaaaactca aagatttgt ctaattaac 
      gccaccgct ttgggtacg gaaattctc aaaagtttg 
      gttttgcct ggcattggt tccttcacg attgttgat 
      ggtgaaaaa gtaactgat gatgatatt ggttcaaac 
      ttttttatc gagagtgat tcaataggg atgtctaga 
      gcacaggta gccactcaa aacctactt gagctgaac 
      cctgatgtt agaggggac tacattgac gagtccgtt 
      gaccacatt atggcccac agtcaagat ttttttgac 
      acgttttct gtcgtaatt gcaacgtgt ttgccagaa 
      aaagtttta atgccgctc tctagacac ttatgggaa 
      aaaaatgta ccacttatt gtatgccgc agtgtgggc 
      tttctgggc tacataaga atacaagtc aaagaacac 
      accataata gaagcccac cctgataac gaaaatcat 
      gatttacgc ttagataat ccatggcct gcacttaaa 
      gaacatttg gataaagtt gatgtaacc aaactagac 
      aacaaggaa cgcagtcat gtgcctgcg gtggttatt 
      ctgtactat tatttggcg cagtttaaa agcaaacac 
      ggccattta ccgaaaact cgagccgaa aaggaagaa 
      gttaaaaaa atgataacg gaaagcccc ccgcctgat 
      gaacacgga attaagaac ttggaggag aattttaaa 
      gaggctatt cgctacatt aatacttgt attaacccc 
      atcaagatt cccccaaat ttgcaagca attctggag 
      gacgatagc tgcataaat gtgaaccag aacagttca 
      cctttttgg gttttatgt gcggccttg aaggaattt 
      gttgaaaaa gaaggggcg ccccctttg aaagggact 
      ttaccagat atggcagct gacaccagt agctacatc 
      acgttgcaa caactctac caaaaacag gctcaaacc 
      cagtgtgaa attatctac aggagggcg ttagaaatt 
      gcgcgaaat ttgggcctc tctcaagag accataacc 
      gaatctgaa gctaagcta ttttgcaag catgcgagt 
      gagttacat gtgattcgt ggcagttgc attgctgat 
      gaatatcaa aaaactaga gtagatcta acgtcatgt 
      cttgaggat cctgatagt ttgatgttc cattacata 
      actctaaga gggctagag cgctttatc agtgaattt 
      aatagttat ccagggcaa ttggatgac catgtggag 
      cctgatgtg cttaaattg aagggcatt attgggaag 
      ttgttagga gagtggggt tgttcacag ataatcaga 
      gatgagagg gtacacgaa gtttgtcgc tacgggggc 
      gctgaactg cactcagtc tcggctatt ttgggcgga 
      tgtgcggct catgaagcc atcaaactt ataactctg 
      cagtataag ccactaaac aacaccttt atttacgat 
      gcaattacc tcaacttct gcctcgttt actctttaa 
      gtggttgat ttgttatta ggttactaa gagtaaagt 
      ttttaaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY