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Gene information
General information
TC005579 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11061036..11065001
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005579
    iBeetle screen iB_08926

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_07329

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005579 RA
      MMTNGAGLQ PPEMLDFFA VHSPTDSSE 
      DSVEVKISP ISGRNKRKC AEPRKVQET 
      FSGPLKKRM CLKVKAESE APFRPWSPK 
      PAGYFRPPS PVRDEPVAL LKKAPAPRV 
      PVHPPPGAS DADAERILR TSAETFPGL 
      GQPRREQRN YKNMTRERR IEANARERT 
      RVHTISAAF DTLRRAIPS YSHNQKLSK 
      LSVLRIACS YIMTLSSIV NGDEGAAAE 
      CVDLVTKTI QKEGKLRKK KDDND
    CDS sequence
    • >TC005579 RA
      atgatgacg aacggcgcc ggcctccag cccccggag 
      atgctcgac ttcttcgcg gtgcactcg cccaccgat 
      tccagcgag gacagcgtc gaggtgaag atctcgccc 
      atctcgggc cgcaacaag cgcaaatgt gccgaaccc 
      cgcaaagtg caggagacc ttctccggg ccgctgaag 
      aagcgcatg tgtctgaaa gtgaaagcg gagagtgag 
      gcgcccttc aggccctgg agccccaag cccgccggc 
      tacttccgc ccgccgtcg cccgtccgc gacgagccc 
      gtggcgctg ctgaagaag gcgcccgcg ccccgggtg 
      ccggtgcac ccgccgccg ggggccagc gacgcggac 
      gccgagcgc atcctccgc acctccgcg gagacgttc 
      cccggcctg gggcagccg cggcgcgag cagaggaac 
      tacaagaac atgaccagg gagcggagg attgaggcc 
      aacgccagg gagaggacg cgagtgcat acgatcagt 
      gcggccttt gacaccctc cggagggcc attccgtcc 
      tattcgcac aaccagaaa ctgtccaag ttgtcggtg 
      ctgcgcatc gcatgctcg tatattatg actttgtcg 
      agtatcgtg aacggggat gagggtgca gccgcggag 
      tgtgtggat ctcgtcacg aaaaccata caaaaggaa 
      ggcaaactc cggaagaag aaagacgac aatgactga
    mRNA sequence
    • >TC005579 RA
      atctgacag aagccattt taacgcata aaaaatctc 
      acaaaaaac ataattgtt aatcgcaag cgctgtcta 
      aagagccaa ttttttcgt taagtaaca gtgcaatta 
      tctaattgg taatgatat caagtacgc gtctgaagg 
      ccgtttctt gacgacact ttctaatgc ccagcgttg 
      gcgccgatt tttcccaac gcttttaat tactgggct 
      ccgtttcgg cgtaaatga cctccgccc cgttccgcc 
      aaaatccaa gtgttcgat taggccctc tttataatc 
      tctccggcg cgcacctgc tgccagaag catgcattc 
      caaacccag caagactgt tacttctca cactaacag 
      cggcgagac agctggcgc caacatagg gcgcccaca 
      cccctccta ttcacattt actacacaa catttcatg 
      tcaacttag tatagtttg gagctccag caagtgcac 
      atcgccgat gctcccggt actatgatg acgaacggc 
      gccggcctc cagcccccg gagatgctc gacttcttc 
      gcggtgcac tcgcccacc gattccagc gaggacagc 
      gtcgaggtg aagatctcg cccatctcg ggccgcaac 
      aagcgcaaa tgtgccgaa ccccgcaaa gtgcaggag 
      accttctcc gggccgctg aagaagcgc atgtgtctg 
      aaagtgaaa gcggagagt gaggcgccc ttcaggccc 
      tggagcccc aagcccgcc ggctacttc cgcccgccg 
      tcgcccgtc cgcgacgag cccgtggcg ctgctgaag 
      aaggcgccc gcgccccgg gtgccggtg cacccgccg 
      ccgggggcc agcgacgcg gacgccgag cgcatcctc 
      cgcacctcc gcggagacg ttccccggc ctggggcag 
      ccgcggcgc gagcagagg aactacaag aacatgacc 
      agggagcgg aggattgag gccaacgcc agggagagg 
      acgcgagtg catacgatc agtgcggcc tttgacacc 
      ctccggagg gccattccg tcctattcg cacaaccag 
      aaactgtcc aagttgtcg gtgctgcgc atcgcatgc 
      tcgtatatt atgactttg tcgagtatc gtgaacggg 
      gatgagggt gcagccgcg gagtgtgtg gatctcgtc 
      acgaaaacc atacaaaag gaaggcaaa ctccggaag 
      aagaaagac gacaatgac tgaagttcc tagctgttg 
      ccattattt gtacttatg taaatagtt tttatttat 
      ttcaacatg acgagttac tttactctg ggttttatt 
      tcaaccttt ggcgaggtg tgggtggaa cagcgagtg 
      cagtatgca agtggtggt atgtttatg tatatgctg 
      gaagtgatc gaaacagat ccagttagt ttgtgccct 
      cgcaaaaaa ttgcataaa atattcact catttattg 
      taaacattg aatcatttt aatgtcagt cgtacaggt 
      cagggacca gtgtttatt tgttggaat aacgcgaaa 
      acaatggcg aaaattttt gttaagtgc ccttcaaaa 
      atttccatt caatatttt atgactaaa ttatctccg 
      taacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY