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Gene information
General information
TC005589 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10978679..10981556
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005589
    iBeetle screen iB_07328

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005589 RA
      MEQLSQIIK NLDLEDVVN ENAGYEYIN VFKNPNKLP 
      INQIHLEKL VVELRKDAP LESIRPDDD FEKQCFERK 
      TQIIPALSK FWLPESQQY FKYQPYNFA LSKDENSSY 
      LANNRHFLQ TLHYLLSCN YHQFWCLVL YEPKVAISL 
      HSFLLSPVL PHQIKNLDD ETCTLYLAV FSQFHRVYQ 
      RLTTFQETE SEYMPDSVG LKFLLEKKL LNLPIVITL 
      FLLYHETNQ VFVNDLVSL YFNETSVFR DKEIEEMLD 
      QVILTLEMI GGHVCGFEP DAVIIPIAV EEKPPVFNL 
      AWVFSVVDY LLNTISSLH MLLKFHKPA INVALSKGI 
      PYRLSFTYE KVFHQLYDF LDGRKELDQ NPKLSEIVF 
      EEIALGRSE FIDVYYAFT SHCLDKTLE FSGDPIHQQ 
      EFVEIYLKL LTTALEDEY FICDYHERY DVGAQNEMF 
      SSCTEIDQT RTDYLLSCI TSLRRHNRL WKTLKSELK 
      TFEKAVLKY ASNRKVKSE IVHENGVGA QAGPSNHVE 
      EVFVPPSEE EIEGSIQMV IDMFPHLGD GFVLQCLEA 
      YNYNTSDVI SAILEENLP PHLVDIPFD LIRIPPEPK 
      PEEPILAYK GRKPGYDDA LKLLNDKKD IKEIKTYIL 
      DGIQYTNEF DDYDDEYDD RADEATVPV PDHGSEDIL 
      KFNPNYEDV VSDTSYSED EYDDEGKPV GASERPHNK 
      ADFCEDPAV IRARREARF RSQGKGQPP RPKPKSDVV 
      GKPKGQGQE KNVLQNRHK KSVNKSSRA NHNRKSGAQ 
      WKRNKGMVP S
    CDS sequence
    • >TC005589 RA
      atggaacaa ctctcccaa ataatcaaa aacctggat 
      ttagaggac gtggtcaat gaaaacgcc ggatatgaa 
      tacatcaac gtcttcaaa aacccgaac aagctgcca 
      ataaaccaa attcattta gaaaaactt gtggttgag 
      ctacgcaaa gacgcccct ttagagtca attaggcca 
      gacgacgat tttgaaaaa caatgtttt gaacgcaaa 
      acccaaata atacctgca cttagcaaa ttttggttg 
      cctgagtcg caacagtac ttcaaatac caaccatac 
      aattttgct ttatcgaaa gacgaaaac tcgtcttat 
      ctggcaaat aatcgccat tttttgcaa acgttgcat 
      tatttgtta agctgcaat tatcaccaa ttctggtgt 
      ctagttttg tacgaaccc aaagtggct attagcctt 
      catagtttt ctactgagc ccagtttta cctcatcag 
      attaaaaat ttagacgat gagacgtgt acgctctat 
      ttagcagta ttttcgcaa ttccaccgg gtttatcaa 
      agactaact acgttccaa gagactgag agtgaatat 
      atgccagac agtgtaggt cttaaattt ttattagaa 
      aagaaacta cttaacctc cccatagta atcacgcta 
      tttctttta taccatgaa accaaccaa gtcttcgtt 
      aacgattta gtttcccta tattttaat gaaacttct 
      gttttccgt gataaagag attgaagaa atgcttgat 
      caagtcatt ttgacgtta gaaatgatc ggtggtcac 
      gtttgtggc tttgaacca gatgctgtg ataatccct 
      atagctgtt gaagagaaa cctccagta tttaatctt 
      gcttgggta ttttcagtc gttgattat ttattaaac 
      acaatttcg tcgttacat atgttactg aaatttcac 
      aaaccggca ataaacgta gcgttatcg aagggaatt 
      ccgtacaga ttatccttc acttatgaa aaagttttc 
      catcaattg tacgatttc ttggacggg agaaaagaa 
      ctggatcaa aaccccaaa ctgtccgaa attgttttc 
      gaagagatt gcgttagga cgctcggaa tttatcgac 
      gtttattac gcctttacg tcacattgc ttggataaa 
      accctggag tttagtggc gacccgata catcagcaa 
      gagtttgtc gagatttat ttgaaatta ttaaccaca 
      gccctcgag gacgagtat tttatctgt gattatcac 
      gaacgttac gatgtgggg gcgcaaaac gaaatgttc 
      agcagttgt accgaaatc gaccaaaca cggactgat 
      taccttctg agttgtata acgagtctg aggcgccac 
      aacagactg tggaaaacg ctaaaaagc gagttaaaa 
      acgtttgaa aaagccgtg ctaaagtac gcctcaaat 
      cgcaaagtc aagtcggag attgtgcac gagaacggc 
      gtgggggcg caagcgggg ccttcgaat cacgtggag 
      gaggtgttt gtgcccccc agtgaggaa gaaatcgaa 
      ggttccatc caaatggtg attgatatg ttcccccat 
      ttgggcgat ggattcgtc ctccagtgc ttggaagcg 
      tacaattat aacacaagt gatgtgatc agtgcaatt 
      cttgaggaa aatctcccg cctcattta gtcgacatt 
      ccatttgac ttaatccgg atacctccg gaacccaag 
      ccggaggaa ccgatcctg gcctacaag ggccggaag 
      ccgggctac gacgacgct ttgaaacta ctaaacgac 
      aagaaagac atcaaggaa atcaaaacg tacattctg 
      gacgggata caatacacc aatgaattc gatgactac 
      gatgacgaa tatgatgat agggctgac gaggccacg 
      gttcctgtc cccgatcac gggtctgaa gacatccta 
      aagttcaac ccgaactac gaggacgtc gtcagtgat 
      acgagctac tcggaggat gagtacgac gacgagggc 
      aagcctgtg ggggcgtcc gagcgcccc cataacaag 
      gctgacttt tgcgaagac ccggctgtt atcagagcg 
      agacgggag gcccgattt aggagtcaa ggcaaaggg 
      cagccgccg cggcccaaa ccaaagagc gatgtggtg 
      gggaaaccg aaagggcaa gggcaagag aagaacgtt 
      ctccaaaat agacacaag aaaagcgtg aataagtcg 
      agtcgggcg aaccataat aggaaaagc ggcgcccag 
      tggaagagg aacaaaggc atggtgcct tcgtaa
    mRNA sequence
    • >TC005589 RA
      ttggcaatt accagacag tgaaaaaaa tccggtagt 
      tttttcaat caagcgaac aattcacgt aaaattatg 
      caataatgg aacaactct cccaaataa tcaaaaacc 
      tggatttag aggacgtgg tcaatgaaa acgccggat 
      atgaataca tcaacgtct tcaaaaacc cgaacaagc 
      tgccaataa accaaattc atttagaaa aacttgtgg 
      ttgagctac gcaaagacg cccctttag agtcaatta 
      ggccagacg acgattttg aaaaacaat gttttgaac 
      gcaaaaccc aaataatac ctgcactta gcaaatttt 
      ggttgcctg agtcgcaac agtacttca aataccaac 
      catacaatt ttgctttat cgaaagacg aaaactcgt 
      cttatctgg caaataatc gccattttt tgcaaacgt 
      tgcattatt tgttaagct gcaattatc accaattct 
      ggtgtctag ttttgtacg aacccaaag tggctatta 
      gccttcata gttttctac tgagcccag ttttacctc 
      atcagatta aaaatttag acgatgaga cgtgtacgc 
      tctatttag cagtatttt cgcaattcc accgggttt 
      atcaaagac taactacgt tccaagaga ctgagagtg 
      aatatatgc cagacagtg taggtctta aatttttat 
      tagaaaaga aactactta acctcccca tagtaatca 
      cgctatttc ttttatacc atgaaacca accaagtct 
      tcgttaacg atttagttt ccctatatt ttaatgaaa 
      cttctgttt tccgtgata aagagattg aagaaatgc 
      ttgatcaag tcattttga cgttagaaa tgatcggtg 
      gtcacgttt gtggctttg aaccagatg ctgtgataa 
      tccctatag ctgttgaag agaaacctc cagtattta 
      atcttgctt gggtatttt cagtcgttg attatttat 
      taaacacaa tttcgtcgt tacatatgt tactgaaat 
      ttcacaaac cggcaataa acgtagcgt tatcgaagg 
      gaattccgt acagattat ccttcactt atgaaaaag 
      ttttccatc aattgtacg atttcttgg acgggagaa 
      aagaactgg atcaaaacc ccaaactgt ccgaaattg 
      ttttcgaag agattgcgt taggacgct cggaattta 
      tcgacgttt attacgcct ttacgtcac attgcttgg 
      ataaaaccc tggagttta gtggcgacc cgatacatc 
      agcaagagt ttgtcgaga tttatttga aattattaa 
      ccacagccc tcgaggacg agtatttta tctgtgatt 
      atcacgaac gttacgatg tgggggcgc aaaacgaaa 
      tgttcagca gttgtaccg aaatcgacc aaacacgga 
      ctgattacc ttctgagtt gtataacga gtctgaggc 
      gccacaaca gactgtgga aaacgctaa aaagcgagt 
      taaaaacgt ttgaaaaag ccgtgctaa agtacgcct 
      caaatcgca aagtcaagt cggagattg tgcacgaga 
      acggcgtgg gggcgcaag cggggcctt cgaatcacg 
      tggaggagg tgtttgtgc cccccagtg aggaagaaa 
      tcgaaggtt ccatccaaa tggtgattg atatgttcc 
      cccatttgg gcgatggat tcgtcctcc agtgcttgg 
      aagcgtaca attataaca caagtgatg tgatcagtg 
      caattcttg aggaaaatc tcccgcctc atttagtcg 
      acattccat ttgacttaa tccggatac ctccggaac 
      ccaagccgg aggaaccga tcctggcct acaagggcc 
      ggaagccgg gctacgacg acgctttga aactactaa 
      acgacaaga aagacatca aggaaatca aaacgtaca 
      ttctggacg ggatacaat acaccaatg aattcgatg 
      actacgatg acgaatatg atgataggg ctgacgagg 
      ccacggttc ctgtccccg atcacgggt ctgaagaca 
      tcctaaagt tcaacccga actacgagg acgtcgtca 
      gtgatacga gctactcgg aggatgagt acgacgacg 
      agggcaagc ctgtggggg cgtccgagc gcccccata 
      acaaggctg acttttgcg aagacccgg ctgttatca 
      gagcgagac gggaggccc gatttagga gtcaaggca 
      aagggcagc cgccgcggc ccaaaccaa agagcgatg 
      tggtgggga aaccgaaag ggcaagggc aagagaaga 
      acgttctcc aaaatagac acaagaaaa gcgtgaata 
      agtcgagtc gggcgaacc ataatagga aaagcggcg 
      cccagtgga agaggaaca aaggcatgg tgccttcgt 
      aagtttgtt tggtttaga aatgttgtg cctttattt 
      caaatgttt aataatttt ttgaataaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY