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Gene information
General information
TC013593 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3308081..3317547
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013593
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07325

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013593 RA
      MCALELKLH SPAGAEPVL YTWPLTSGR GNDKHDGAL 
      EIVETIRWV CEDLPELKL PLENNILCD YDTKDYESM 
      KNLCDRFNR AIDSVVALE KGTSLIAQR LNKYPSRGL 
      LRHILQQTY NAAVTDPEK LNQYEPFSP EVYGETSYD 
      LVCQMIDQI DVTSDDVFI DLGSGVGQV VLQMAAATP 
      CKVCFGIER AEVPSRYAE AMNKYFRTW MSWYGKRYG 
      EYKLIRSDF LTEEHREKI NQATIVFVN NFAFGPAVD 
      HQLKERFAD LKDGARIVS SKSFCPLNF RITDRNLSD 
      IGTIMHVSE MTPMKGSVS WTGKPVSYY LHRIDRTKL 
      ERYFQRMKK PQLKNGDDN DNSRSAREK AKRDLSKQL 
      GSSSDSDYD DQDSNYGPT TRKAWSDYC STKGKSSQS 
      EEEGNNKNG KRQPKKLRR KIQRGKNQA NGEKAQKTG 
      PKSRRGRVK KGGKPKKTI KINGLDLLH NETLLSTSP 
      QAIGKKLPP APGCVDQQL TALSTELVH NELDIPQAP 
      ASTPYGLQI LLDMFRAQY MQMLNQMKQ PTYKTSVEN 
      HIKQEKERN KKLQNRAAQ LEKQIKVLI DDSVALLKA 
      RMSELGINA TSPVDLLAK AKEIVCRHK ELQAKASKL 
      QGQVASLEQ EQNNLVIQR QQEIVEKYN GRDTCHDLS 
      PIATQEYIL REISSTLAH RKKLHNQVS RLEGELLSL 
      EKANEERKQ AQALATRQQ QTPSTKSSR KSREYRARS 
      QDWPDVPDI AKIEEQNPE ILAQKILET GRKIEASKM 
      SANCRKINL VRNNMPAPL PVPKRQKTS YSLPPTQSS 
      ATRAQEPPR LADFEDRLK SIITSALNE DQQNRNRAQ 
      SAYQQQQNY VNFQNDKHK FAKYNRCET VVKQNEERS 
      FAQQEGLAG MMESYRTEV PKVSPHVAR QVAPEQPDY 
      TQVSPAKLA LRRHLSQEK LAAQHHSFS SSDGIVATR 
      TIGDLMSGE IERTLEISN QSIINAAVD MSDIQGTTT 
      LTSRSVVNV NLPPRPERV KVNNETKKA EPMRQVYSP 
      ISRPSSTEG LEGLAYTPL PRADIRPYH ESYFTDVKP 
      PIEGEIRGN FVAEGLAAS LQARVLSGP QIKEEVDVY 
      DRRSCVQPN VLYQDHNGS TIKSEGVSD CQYKRESPI 
      IQGPIRNSL KRRLVDEQE PARVRKCNE EDADVEEEG 
      EESKWQDRI SSGFDRLVA FASTELDKR RRSTEGSDS 
      CNTSPDSGI GHGDLPPSS LTVSTPAKQ ALKLNPNSK 
      PTLFKMPNK IYLESPPLI GESGNDESG PPRTPSPSS 
      LISVPLNYS PEPPNEKSQ PRLNPALLK YQREDKKVK 
      PDQHYKKKF YYREQWRDD SWQNRDHDR YCSWKN
    CDS sequence
    • >TC013593 RA
      atgtgtgcg ctggaattg aaactgcat tctccggcg 
      ggggcagaa ccagtatta tacacttgg ccgttaacc 
      tcaggacga ggaaacgac aaacatgat ggtgccctt 
      gagatcgtc gaaaccatc agatgggtc tgtgaagat 
      ctgccggag cttaaactg ccgttggaa aacaacatt 
      ctctgtgat tatgatacg aaagattac gagagtatg 
      aaaaatctc tgcgatcgg ttcaatcgg gccattgac 
      agcgttgtt gctttggag aaaggaacg tcgctgata 
      gcccaaagg ctgaacaag tacccgtcc agagggctc 
      ctccgccac atcctccag caaacgtac aacgctgcc 
      gtcaccgac cccgaaaaa ctgaaccaa tatgaaccg 
      ttctcccct gaagtgtac ggcgaaaca tcctacgat 
      ctagtctgc caaatgatc gatcaaatc gacgtgaca 
      tccgacgac gtgttcatc gacttgggc tcaggagtg 
      ggtcaagtg gttctccaa atggctgcg gcgacgccg 
      tgcaaagtg tgcttcggc atagaacgg gccgaagtt 
      ccaagcaga tacgccgaa gccatgaac aagtatttc 
      cggacgtgg atgtcctgg tacgggaag cgatacgga 
      gagtacaaa ttaataagg agcgatttt ttgacggag 
      gaacaccgc gagaaaatc aaccaagca acgatcgtt 
      tttgttaac aatttcgcg ttcggaccg gctgttgat 
      caccagctg aaagagcgg ttcgccgat ttgaaggac 
      ggagctagg atcgtttcg tcgaagagt ttttgtccg 
      ctgaatttt cggatcacc gatcggaat ttaagcgac 
      atcgggacg ataatgcac gtttcggag atgacgcca 
      atgaaggga tcggtgtcc tggactggg aaaccagtc 
      tcttattac ttgcacagg attgatcgg acgaagctg 
      gaacggtat ttccaaagg atgaagaag ccacagctc 
      aaaaacggt gacgataat gacaattca cgtagtgcg 
      cgtgaaaag gctaagaga gatctttct aaacagttg 
      gggtcgtca tctgattcc gactatgac gaccaagac 
      agtaattat ggacctacg acacgcaaa gcttggtcc 
      gattattgt tccactaag ggtaaaagt agtcagtcg 
      gaagaggag ggtaacaac aagaatgga aagcgacag 
      ccgaagaaa ttacggaga aaaatacaa cgtgggaaa 
      aatcaagcc aatggtgaa aaggcacaa aagacggga 
      ccgaaatct cgcagagga agagtgaag aaaggtggg 
      aaaccaaag aagacgatc aaaatcaac ggcttggat 
      ctgctacac aacgaaaca cttctgagt acatccccg 
      caagcgata ggtaaaaag cttccacca gcacctggt 
      tgtgtcgat cagcaatta acagcatta agtactgaa 
      ctcgttcac aatgaatta gacatacct caagcccca 
      gcaagtaca ccgtacggt ctccagatt cttttagac 
      atgtttaga gctcaatat atgcaaatg ttgaatcaa 
      atgaaacaa ccgacttac aaaaccagc gtcgagaat 
      catatcaaa caagagaaa gaaagaaat aagaaactg 
      caaaaccgt gccgcccaa ctagaaaag caaataaag 
      gtgttaatc gatgacagt gtcgcactc ctgaaggcc 
      cgtatgagc gaactggga ataaacgca acatcaccg 
      gtcgatctc ctagcaaaa gccaaggaa atcgtgtgc 
      cgccataaa gaactccaa gcgaaagca agcaaattg 
      caaggccaa gtcgccagt ttggagcaa gaacagaac 
      aatctggtc atccaacga caacaagaa atcgtcgaa 
      aagtacaac ggacgcgac acttgccac gacttatcg 
      ccgatcgcg acccaagaa tacatttta cgcgaaata 
      tcctcaacc ctcgcccac cgcaaaaaa ctccacaat 
      caagtgtcg cgtctcgaa ggcgagctc ctctcccta 
      gagaaagcc aacgaggag cgcaaacaa gcacaagct 
      ctagccacg cgccagcaa caaacaccc agcacgaaa 
      tcgtcgcga aaatcccgc gaataccgc gccaggtcc 
      caagactgg cccgacgtg cctgacatc gccaaaatc 
      gaagaacaa aaccccgaa atcctcgcc cagaaaatc 
      ctcgaaacg gggcgcaaa atcgaagcc agtaaaatg 
      tcagcgaac tgccggaaa atcaacctc gtccgcaac 
      aacatgccg gccccctta ccggtgcca aagcgtcaa 
      aaaacgagc tattcgcta ccaccaacc cagagcagc 
      gcaactcgg gcccaagaa ccgccaaga ctggccgac 
      ttcgaggac cggctcaaa agcatcatc acgtcggca 
      ctgaacgaa gaccagcaa aaccgcaac cgggcgcag 
      tctgcctac cagcagcag caaaactac gtcaatttc 
      cagaacgac aaacacaag tttgctaaa tacaatcgg 
      tgcgaaacc gtggtcaaa cagaacgag gagcgcagt 
      ttcgcccaa caggaaggt ctcgccggc atgatggag 
      tcgtaccgg acggaagtg ccgaaagtc tcgccgcat 
      gtggcaaga caagtggcg ccggaacag cccgactat 
      acccaagtg tctccggcc aagctagcg ctgagacgc 
      cacctgtcc caggagaag ttggccgca caacaccac 
      agttttagc tcgtcggac ggcatcgtc gccacgcga 
      accatcggc gatttaatg tccggcgag attgaacgg 
      acgttggag atctcaaac cagtcgatc atcaacgct 
      gccgtcgac atgagtgac atccaaggc acaacgacg 
      ctgacatcg agatccgtg gtcaatgtg aacctcccc 
      ccgcggccg gagcgcgtc aaagtcaac aacgagacg 
      aagaaagcg gagccgatg cgccaggtc tacagcccg 
      atatcgcgg ccgtcgtcc accgagggt ctggaaggc 
      ttggcgtac acgccgctt ccgcgcgcc gatatccgg 
      ccgtaccac gagtcgtac tttaccgac gtcaagccg 
      ccgattgag ggcgaaatc cgcggcaat ttcgtggcg 
      gaaggcttg gctgccagc ttgcaagcg cgtgtctta 
      agcggcccg caaatcaag gaagaggtg gacgtttac 
      gaccggaga agttgcgtg caaccaaat gtgctatac 
      caggaccat aatggctcg actatcaaa agcgagggc 
      gtcagtgat tgtcagtac aaacgggag tcgccgatt 
      atacaaggg ccgattcgc aattcgctg aagcggcgg 
      ctagtggac gagcaagag ccggcacgg gtccggaag 
      tgcaacgaa gaagatgca gatgtcgaa gaagagggc 
      gaagaatca aaatggcag gaccgtatt agttcaggt 
      ttcgatcgc ttggttgct ttcgcatcc accgagtta 
      gataaacgg aggcgatcg actgagggt agcgacagt 
      tgtaacacg tccccagat tcgggaata ggtcacggt 
      gatttacca ccgtcctct ttaacagtg tccactccg 
      gcgaaacag gcgttgaaa ttaaaccca aactcgaaa 
      ccgactctt tttaaaatg ccgaacaaa atttattta 
      gagagtcct cccctaata ggggagagt ggaaacgac 
      gaatctgga ccacctcga accccgtct ccttcttcg 
      ctcatcagc gttcctcta aattacagc ccggaacca 
      ccaaatgag aaaagccag ccacgtctg aatccggct 
      ttacttaag taccaacgc gaggataaa aaagttaaa 
      ccggatcaa cactacaag aagaaattt tattacaga 
      gaacagtgg agggatgat tcttggcaa aatcgcgac 
      cacgaccgg tattgctcg tggaaaaat tga
    mRNA sequence
    • >TC013593 RA
      tcgataata gagcaggta caagtccga tgcaaacgg 
      aattccgta aactaccaa aactgtcag caataaatg 
      atgttgggt tggtagtac tgtcatagc acttgcgcg 
      cttaacgtg tatgttcgg cgatggcgt gttttgctg 
      tgtgaaatc tatttaatt gttaaaaaa tgtgtttat 
      aaagtggtt tagttagtt caaatggtg ttcatattt 
      agtgctaag ggctgcgtt agttgatat cgacccatg 
      tgtgcgctg gaattgaaa ctgcattct ccggcgggg 
      gcagaacca gtattatac acttggccg ttaacctca 
      ggacgagga aacgacaaa catgatggt gcccttgag 
      atcgtcgaa accatcaga tgggtctgt gaagatctg 
      ccggagctt aaactgccg ttggaaaac aacattctc 
      tgtgattat gatacgaaa gattacgag agtatgaaa 
      aatctctgc gatcggttc aatcgggcc attgacagc 
      gttgttgct ttggagaaa ggaacgtcg ctgatagcc 
      caaaggctg aacaagtac ccgtccaga gggctcctc 
      cgccacatc ctccagcaa acgtacaac gctgccgtc 
      accgacccc gaaaaactg aaccaatat gaaccgttc 
      tcccctgaa gtgtacggc gaaacatcc tacgatcta 
      gtctgccaa atgatcgat caaatcgac gtgacatcc 
      gacgacgtg ttcatcgac ttgggctca ggagtgggt 
      caagtggtt ctccaaatg gctgcggcg acgccgtgc 
      aaagtgtgc ttcggcata gaacgggcc gaagttcca 
      agcagatac gccgaagcc atgaacaag tatttccgg 
      acgtggatg tcctggtac gggaagcga tacggagag 
      tacaaatta ataaggagc gattttttg acggaggaa 
      caccgcgag aaaatcaac caagcaacg atcgttttt 
      gttaacaat ttcgcgttc ggaccggct gttgatcac 
      cagctgaaa gagcggttc gccgatttg aaggacgga 
      gctaggatc gtttcgtcg aagagtttt tgtccgctg 
      aattttcgg atcaccgat cggaattta agcgacatc 
      gggacgata atgcacgtt tcggagatg acgccaatg 
      aagggatcg gtgtcctgg actgggaaa ccagtctct 
      tattacttg cacaggatt gatcggacg aagctggaa 
      cggtatttc caaaggatg aagaagcca cagctcaaa 
      aacggtgac gataatgac aattcacgt agtgcgcgt 
      gaaaaggct aagagagat ctttctaaa cagttgggg 
      tcgtcatct gattccgac tatgacgac caagacagt 
      aattatgga cctacgaca cgcaaagct tggtccgat 
      tattgttcc actaagggt aaaagtagt cagtcggaa 
      gaggagggt aacaacaag aatggaaag cgacagccg 
      aagaaatta cggagaaaa atacaacgt gggaaaaat 
      caagccaat ggtgaaaag gcacaaaag acgggaccg 
      aaatctcgc agaggaaga gtgaagaaa ggtgggaaa 
      ccaaagaag acgatcaaa atcaacggc ttggatctg 
      ctacacaac gaaacactt ctgagtaca tccccgcaa 
      gcgataggt aaaaagctt ccaccagca cctggttgt 
      gtcgatcag caattaaca gcattaagt actgaactc 
      gttcacaat gaattagac atacctcaa gccccagca 
      agtacaccg tacggtctc cagattctt ttagacatg 
      tttagagct caatatatg caaatgttg aatcaaatg 
      aaacaaccg acttacaaa accagcgtc gagaatcat 
      atcaaacaa gagaaagaa agaaataag aaactgcaa 
      aaccgtgcc gcccaacta gaaaagcaa ataaaggtg 
      ttaatcgat gacagtgtc gcactcctg aaggcccgt 
      atgagcgaa ctgggaata aacgcaaca tcaccggtc 
      gatctccta gcaaaagcc aaggaaatc gtgtgccgc 
      cataaagaa ctccaagcg aaagcaagc aaattgcaa 
      ggccaagtc gccagtttg gagcaagaa cagaacaat 
      ctggtcatc caacgacaa caagaaatc gtcgaaaag 
      tacaacgga cgcgacact tgccacgac ttatcgccg 
      atcgcgacc caagaatac attttacgc gaaatatcc 
      tcaaccctc gcccaccgc aaaaaactc cacaatcaa 
      gtgtcgcgt ctcgaaggc gagctcctc tccctagag 
      aaagccaac gaggagcgc aaacaagca caagctcta 
      gccacgcgc cagcaacaa acacccagc acgaaatcg 
      tcgcgaaaa tcccgcgaa taccgcgcc aggtcccaa 
      gactggccc gacgtgcct gacatcgcc aaaatcgaa 
      gaacaaaac cccgaaatc ctcgcccag aaaatcctc 
      gaaacgggg cgcaaaatc gaagccagt aaaatgtca 
      gcgaactgc cggaaaatc aacctcgtc cgcaacaac 
      atgccggcc cccttaccg gtgccaaag cgtcaaaaa 
      acgagctat tcgctacca ccaacccag agcagcgca 
      actcgggcc caagaaccg ccaagactg gccgacttc 
      gaggaccgg ctcaaaagc atcatcacg tcggcactg 
      aacgaagac cagcaaaac cgcaaccgg gcgcagtct 
      gcctaccag cagcagcaa aactacgtc aatttccag 
      aacgacaaa cacaagttt gctaaatac aatcggtgc 
      gaaaccgtg gtcaaacag aacgaggag cgcagtttc 
      gcccaacag gaaggtctc gccggcatg atggagtcg 
      taccggacg gaagtgccg aaagtctcg ccgcatgtg 
      gcaagacaa gtggcgccg gaacagccc gactatacc 
      caagtgtct ccggccaag ctagcgctg agacgccac 
      ctgtcccag gagaagttg gccgcacaa caccacagt 
      tttagctcg tcggacggc atcgtcgcc acgcgaacc 
      atcggcgat ttaatgtcc ggcgagatt gaacggacg 
      ttggagatc tcaaaccag tcgatcatc aacgctgcc 
      gtcgacatg agtgacatc caaggcaca acgacgctg 
      acatcgaga tccgtggtc aatgtgaac ctccccccg 
      cggccggag cgcgtcaaa gtcaacaac gagacgaag 
      aaagcggag ccgatgcgc caggtctac agcccgata 
      tcgcggccg tcgtccacc gagggtctg gaaggcttg 
      gcgtacacg ccgcttccg cgcgccgat atccggccg 
      taccacgag tcgtacttt accgacgtc aagccgccg 
      attgagggc gaaatccgc ggcaatttc gtggcggaa 
      ggcttggct gccagcttg caagcgcgt gtcttaagc 
      ggcccgcaa atcaaggaa gaggtggac gtttacgac 
      cggagaagt tgcgtgcaa ccaaatgtg ctataccag 
      gaccataat ggctcgact atcaaaagc gagggcgtc 
      agtgattgt cagtacaaa cgggagtcg ccgattata 
      caagggccg attcgcaat tcgctgaag cggcggcta 
      gtggacgag caagagccg gcacgggtc cggaagtgc 
      aacgaagaa gatgcagat gtcgaagaa gagggcgaa 
      gaatcaaaa tggcaggac cgtattagt tcaggtttc 
      gatcgcttg gttgctttc gcatccacc gagttagat 
      aaacggagg cgatcgact gagggtagc gacagttgt 
      aacacgtcc ccagattcg ggaataggt cacggtgat 
      ttaccaccg tcctcttta acagtgtcc actccggcg 
      aaacaggcg ttgaaatta aacccaaac tcgaaaccg 
      actcttttt aaaatgccg aacaaaatt tatttagag 
      agtcctccc ctaataggg gagagtgga aacgacgaa 
      tctggacca cctcgaacc ccgtctcct tcttcgctc 
      atcagcgtt cctctaaat tacagcccg gaaccacca 
      aatgagaaa agccagcca cgtctgaat ccggcttta 
      cttaagtac caacgcgag gataaaaaa gttaaaccg 
      gatcaacac tacaagaag aaattttat tacagagaa 
      cagtggagg gatgattct tggcaaaat cgcgaccac 
      gaccggtat tgctcgtgg aaaaattga taaattcgc 
      gcggttgat tcattaatt actacttta tttgcgatt 
      tcacacagc aacaaggtt gaagcggtt tgacttgta 
      tagttaatt tatacactg tatttattt tatttatac 
      tatttatca cgtctatct gagctttat gctttcgtc 
      tggtgactt gcttcacgt ttaaatatt tttggcttc 
      cagttttac cgttaaacc caacattcg tcatattat 
      gtgttcacg agggactaa tatttaaac gtgtatgtg 
      agtcgttac tgtacctac gaattacga atcgttgtg 
      gtgttcaaa ttcgaaact ttggtttga agttttggt 
      gtccggacc actgcttca aatctgatg catcgtttg 
      caccaagtg ctagatgtg gtgtagatg ttcttcttt 
      tttactaaa tagatggtt gacggacgg tcacgctag 
      agaattttc ggtcaacta ggtaaattg tacatttat 
      gttttagcg taaataatg gagactgta actttgtgt 
      tcatgaaaa ttggaaggt ttatcgagt ttcactata 
      atttattca atttgcttg taagggtat attaattta 
      tactaacgt attgaagac aaacactgc ctattaaat 
      ctcataaaa ctgttttca caattatta agtataatt 
      tatttgtac attgtacat atgttttta gactagagc 
      tagcgagtt aggatacat attgtaaaa agtatagtt 
      aatttaaac ataggtaat ccacgtggg gaagtcgtt 
      gcctatgtt ttatattca agaccgatt tttacgtac 
      aagtgtaat actgccatt tcatcatta atttcatac 
      atttatatg agaagtatt cgtcattcg acttgtact 
      tgtttaaag tgttatgct tgtaactga ttttaacgt 
      agttcattc gcacggtcg gttttcaat cgtattttt 
      tcaagtggg gtttttaac tttgtgtac aaagactag 
      gaactgtca ttgcaaaca ttttaaaca tacttgtat 
      atagttgag atcacagat taagtcagt ttttattac 
      gttggactt attttaacc tatatcgca attttgcaa 
      atacagttt ttaacttcg tattcggtc cctttttat 
      attaataat caaagtttt tttaagtga tattttgcc 
      tattttgta caaaagtac ttttgttat ttttatggt 
      cttttgtct tttatcagt ttcatttgt tgtctttcg 
      cattatatt atagtttat ttttttagt actaaactt 
      taatgttaa tactttttc aagttttgt ttgttgaat 
      tttatttct tcgcaccat attattact gtcatatcc 
      ttgaatcag actttcgtt tgacatttc atgaaaccc 
      attatgaag aagccaaag ttttatcta attcgacgt 
      tttgtgact gaatttact ttttttaat tcatggttg 
      aaaatagaa aggatagct ctcaggatt actatgaat 
      gtgaataag actaggaat agaatgtat gttacatgt 
      aaacaataa gttggtaat tatggtatc cttttaact 
      gccattaat ttaataaac acttctatg tatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY