Status
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Gene information
General information
TC012552 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11350296..11365545
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012552
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07324

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    egg chamber number decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 1

    vitellarium potentially size decreased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012552 RA
      MKSKNKSDA KVASEQLIL AEKPPLDKK KLAALEKER 
      LKAEKKLKK GKTLCQACK KKCSGEVLR VQDKYFHTQ 
      CFKCKVCGN SLAQGGFFS KDGAYYCTA DYQRNFGTK 
      CATCHDYVE GEVVTALGK TYHKKCFTC DRCRQPPPD 
      EKVTYTGKE ILCQKCVQI PVRDGTPKT SPTSNNANE 
      CAGCGKELN EGQALVALD QQWHIWCFK CGTCGVVLH 
      GEYMGRNGQ PYCERDYQK QFGIKCAYC SRYISGKVL 
      QAGDNHHFH PTCARCTKC GDPFGDGEE MYLQGGAIW 
      HPRCGPGPT ENGQILNGS GEPNGHCTD TDASVREFD 
      RMSSSGVSE MQFSMRSRT PSLNGSLYS PTSMSRKHF 
      SYRTPSPGL ILREHNKSA TLADDISRI YTYSYLTEE 
      PTLGYLRRP IDPYDKAPT SPHFHRPTS QNSLRGSQS 
      GRKSHGSRS AMKVLVDSI RSETPRPKS PHMNNEEPI 
      ELAHFPAAK PPQPGEPPK IERDDFPAP PYPYTDPER 
      RKRWSDSYK GVPDSDEDE VDSSAPKIE DLKLKKEEE 
      ELSKISSGI GKVFLKDVK DREKLRQWK AAHLDPRNA 
      SRTPSANKE PAYRLRYDN PIGASPSRN MDHPKPFDE 
      DDYDRSYSG RSSVGRSIN YNVVSSLRS VPRPGYGLK 
      SSTLPARNG YGDYSFSGM GAKTHSTDF SCGKSDIST 
      GSITDVERR ALNSEMPVS ATYMGALGR YPGGYSSQV 
      RRSLPNMAH TMLINEPAK IYPYHLLVI TNYRLPSDV 
      DRCNLERHL SDAEFEAIF QVSRTDFYR LPQWRRNEL 
      KRRARLF
    CDS sequence
    • >TC012552 RA
      atgaaaagc aaaaacaaa agcgacgcc aaagtggcc 
      agcgagcag ctgatcctg gccgaaaag ccgcccctt 
      gacaagaaa aagctggcc gccctcgag aaagagcgc 
      ctcaaagcc gaaaagaag ctgaaaaaa ggtaaaact 
      ctatgccag gcatgcaag aagaagtgt tccggcgag 
      gtcctccgc gtccaagac aagtacttc cacacgcaa 
      tgcttcaag tgcaaagtg tgtggaaac tccctggcc 
      caaggcgga ttcttctcc aaagacggt gcttattac 
      tgtacggcc gactaccag aggaacttc ggcacgaaa 
      tgcgccact tgccacgac tacgtcgag ggcgaggtg 
      gtgaccgct ctggggaag acctaccac aagaagtgc 
      ttcacgtgc gatcggtgc cggcagccg ccccctgac 
      gaaaaagtc acctacacg gggaaggag atactctgc 
      cagaaatgt gttcagatc ccggtgcgg gacgggacg 
      cccaaaact agccccacg agtaataat gccaatgaa 
      tgtgccggc tgcggcaaa gaactgaac gaaggacag 
      gctctggtg gcgttagac cagcagtgg cacatttgg 
      tgcttcaag tgcggcact tgtggcgtg gttttgcac 
      ggggagtac atggggagg aatggacag ccgtactgc 
      gagagggat taccagaag cagttcggg attaagtgt 
      gcttactgc agcagatac atcagcggg aaggtgctt 
      caggctggt gacaaccac catttccac ccgacttgc 
      gcccgctgc acgaaatgc ggggacccc ttcggggac 
      ggcgaggag atgtacctc caagggggc gccatctgg 
      cacccgcgc tgcggcccc ggccccacg gaaaacggc 
      caaattttg aacggttca ggtgagccc aacggccac 
      tgcaccgac accgatgct tctgtgcga gagttcgac 
      agaatgagt agttccgga gtgagcgaa atgcagttc 
      tccatgcgc tctcgcacc cctagcttg aacggttcg 
      ttatacagt cccacaagc atgtctaga aagcatttc 
      agttaccga acaccatcg cccggcttg atattaaga 
      gagcataac aaatcagcg actctagcc gacgatatt 
      tccagaatt tatacttat agttacctt acggaagaa 
      ccgacttta gggtattta cgcagacct attgatccc 
      tatgataag gctccgacg tcgccacat ttccataga 
      ccgacttcc caaaattcg ttaaggggg agtcaaagt 
      ggccgaaaa agtcacggt tcgaggtca gcgatgaaa 
      gttttagtt gattcgatt agaagtgaa acaccaagg 
      cctaaatcg ccgcacatg aataacgaa gagccaata 
      gaattagcg catttcccg gctgccaaa ccgccacaa 
      cccggggaa ccaccgaaa atcgagagg gacgatttt 
      ccagcgccg ccctacccc tacaccgac ccagaacgt 
      cggaaaaga tggtccgac tcgtacaaa ggcgtgccc 
      gactcggac gaggacgag gtggactcc agcgccccg 
      aaaatcgaa gacctgaag ctgaagaag gaagaagaa 
      gagctgagc aaaatatcc agcggcatc ggaaaagtc 
      ttcctcaaa gacgtcaaa gaccgcgag aaattgcgc 
      caatggaag gccgcccat ttagacccg cggaacgcc 
      tccaggaca ccttcggcg aacaaagaa ccggcgtac 
      agattgaga tatgacaac ccgatcggg gccagtcct 
      tcgcggaat atggaccac cccaagccc ttcgacgag 
      gacgactat gaccggtca tacagcggc cggtcatcg 
      gtgggacgc tccataaat tataacgta gtctcatca 
      ttgcgaagt gtccctaga ccaggttac gggttaaaa 
      tcgtcgaca ctgcctgct agaaacgga tatggcgat 
      tattcgttt agcggaatg ggtgcaaaa acccatagc 
      actgacttc agttgtgga aaatccgac atttccact 
      ggatccatc acggatgtt gaaagacgt gctctgaat 
      tccgaaatg cccgtgtcg gcgacttac atgggggcc 
      ctggggcgc tacccgggc gggtactca tcacaagtg 
      cgacggtcc cttccaaac atggcccac acgatgctg 
      ataaacgaa ccggccaaa atctaccct taccacttg 
      cttgtgatc actaattac agactgcct tctgatgtc 
      gacagatgc aatcttgag cgacatctg tcggacgcc 
      gagttcgag gcgattttc caagtctcg cggaccgac 
      ttctaccga ctaccccaa tggcgcagg aacgaacta 
      aaacgtaga gctcgcttg ttttaa
    mRNA sequence
    • >TC012552 RA
      gtgcttttg ctttcagta gagagtttc ctgctgttc 
      ggagcgcgc aaagtcggt acatgtgta attagtgtc 
      gaaaaatat gaaaagcaa aaacaaaag cgacgccaa 
      agtggccag cgagcagct gatcctggc cgaaaagcc 
      gccccttga caagaaaaa gctggccgc cctcgagaa 
      agagcgcct caaagccga aaagaagct gaaaaaagg 
      taaaactct atgccaggc atgcaagaa gaagtgttc 
      cggcgaggt cctccgcgt ccaagacaa gtacttcca 
      cacgcaatg cttcaagtg caaagtgtg tggaaactc 
      cctggccca aggcggatt cttctccaa agacggtgc 
      ttattactg tacggccga ctaccagag gaacttcgg 
      cacgaaatg cgccacttg ccacgacta cgtcgaggg 
      cgaggtggt gaccgctct ggggaagac ctaccacaa 
      gaagtgctt cacgtgcga tcggtgccg gcagccgcc 
      ccctgacga aaaagtcac ctacacggg gaaggagat 
      actctgcca gaaatgtgt tcagatccc ggtgcggga 
      cgggacgcc caaaactag ccccacgag taataatgc 
      caatgaatg tgccggctg cggcaaaga actgaacga 
      aggacaggc tctggtggc gttagacca gcagtggca 
      catttggtg cttcaagtg cggcacttg tggcgtggt 
      tttgcacgg ggagtacat ggggaggaa tggacagcc 
      gtactgcga gagggatta ccagaagca gttcgggat 
      taagtgtgc ttactgcag cagatacat cagcgggaa 
      ggtgcttca ggctggtga caaccacca tttccaccc 
      gacttgcgc ccgctgcac gaaatgcgg ggacccctt 
      cggggacgg cgaggagat gtacctcca agggggcgc 
      catctggca cccgcgctg cggccccgg ccccacgga 
      aaacggcca aattttgaa cggttcagg tgagcccaa 
      cggccactg caccgacac cgatgcttc tgtgcgaga 
      gttcgacag aatgagtag ttccggagt gagcgaaat 
      gcagttctc catgcgctc tcgcacccc tagcttgaa 
      cggttcgtt atacagtcc cacaagcat gtctagaaa 
      gcatttcag ttaccgaac accatcgcc cggcttgat 
      attaagaga gcataacaa atcagcgac tctagccga 
      cgatatttc cagaattta tacttatag ttaccttac 
      ggaagaacc gactttagg gtatttacg cagacctat 
      tgatcccta tgataaggc tccgacgtc gccacattt 
      ccatagacc gacttccca aaattcgtt aagggggag 
      tcaaagtgg ccgaaaaag tcacggttc gaggtcagc 
      gatgaaagt tttagttga ttcgattag aagtgaaac 
      accaaggcc taaatcgcc gcacatgaa taacgaaga 
      gccaataga attagcgca tttcccggc tgccaaacc 
      gccacaacc cggggaacc accgaaaat cgagaggga 
      cgattttcc agcgccgcc ctaccccta caccgaccc 
      agaacgtcg gaaaagatg gtccgactc gtacaaagg 
      cgtgcccga ctcggacga ggacgaggt ggactccag 
      cgccccgaa aatcgaaga cctgaagct gaagaagga 
      agaagaaga gctgagcaa aatatccag cggcatcgg 
      aaaagtctt cctcaaaga cgtcaaaga ccgcgagaa 
      attgcgcca atggaaggc cgcccattt agacccgcg 
      gaacgcctc caggacacc ttcggcgaa caaagaacc 
      ggcgtacag attgagata tgacaaccc gatcggggc 
      cagtccttc gcggaatat ggaccaccc caagccctt 
      cgacgagga cgactatga ccggtcata cagcggccg 
      gtcatcggt gggacgctc cataaatta taacgtagt 
      ctcatcatt gcgaagtgt ccctagacc aggttacgg 
      gttaaaatc gtcgacact gcctgctag aaacggata 
      tggcgatta ttcgtttag cggaatggg tgcaaaaac 
      ccatagcac tgacttcag ttgtggaaa atccgacat 
      ttccactgg atccatcac ggatgttga aagacgtgc 
      tctgaattc cgaaatgcc cgtgtcggc gacttacat 
      gggggccct ggggcgcta cccgggcgg gtactcatc 
      acaagtgcg acggtccct tccaaacat ggcccacac 
      gatgctgat aaacgaacc ggccaaaat ctaccctta 
      ccacttgct tgtgatcac taattacag actgccttc 
      tgatgtcga cagatgcaa tcttgagcg acatctgtc 
      ggacgccga gttcgaggc gattttcca agtctcgcg 
      gaccgactt ctaccgact accccaatg gcgcaggaa 
      cgaactaaa acgtagagc tcgcttgtt ttaaactct 
      tcatgtgct actagatgc tgcttgagt gctaaacca 
      ccacgctaa ggctgctat acactgcga tgtgtaact 
      ggttttctt tgagtgttc cttgattca tttaccgac 
      tggatcatt cttcgccat agtttaagt tgacaaggt 
      ttctgcgaa atgtgctag agtagctct tggtttcct 
      tacggatta ttccaacta gataaaaaa caatatttt 
      tctactgat ttgaactat tataatctg tattgtatt 
      ggtgaaaaa gagtatctt ggaagattc aacaatttt 
      ttaatcata ggacaaatt ggctatatt cagagattg 
      atatttttt ctataggat aagtgtata actaggtag 
      tgctaagat agttgaaag ttatttata aaaacggca 
      tttagattg ctttaatta ttgctttgg gctagtcgt 
      atattcttt tgtacattt gttagtgct gtcaaggac 
      tttgtggta ccttttatt tctggtata ttgttttaa 
      acccaaatt cattttcat gagtgtttc tattgttac 
      aaattattt atttattct cataaaata acgatttta 
      aagctttag gtatctaag atttgttaa taattgctc 
      tgataaata aaaaacttt taaatac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY