Status
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Gene information
General information
TC016365 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7102327..7104774
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016365
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07320

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016365 RA
      MLKTKLLKF ANTSQKILM STGSENVIS QKKLDHYKN 
      LNALENSDV MPEGWDKAK PFESLPGPK PIPFFGNIW 
      RFLVPKIGD FYGIDFLDL HKIFYEQYG DISILKGMI 
      NDPIVVIFN PKDYETLFR NEGIWPIRK GLQSFNEYR 
      KSRKDIFVN AGLLLEEGE NWFKIRSVV NPILMQPRN 
      ISQYTDKMN SVADELVNN ITNLLEESE NGEMPENFH 
      NELYKWSLE SVGLVTLDT HLGCLKNDL DEDSEPQKL 
      IRSTLEMFK LMHKLDVLP SFHNYVSTP SWRKFVQVM 
      DFIVETNMK YVNQVLDKL EKGETTSTE IPSVLEKLL 
      KVDRNIAVT MSIDMMIAG IDTTGRILG AALYFLGKN 
      HEAQEKLRS EAISLLQTK DQAVTSEVL KKAPYLKAV 
      IKETTRLAP IGIGNLRTT VKNLVLGGY QIPKGTDVV 
      TSNLVLCTN DEYFSRAKE FIPERWLST TSGELSKKN 
      TNPFIFAPF GYGPRSCVG KRLASLELE VALLKIIRN 
      FELDWPHED MVFKTKMLY GMTEPLKIH VRSV
    CDS sequence
    • >TC016365 RA
      atgttaaaa acgaagtta ctgaagttt gccaacact 
      tcgcaaaag attttgatg tcaacgggc agtgaaaat 
      gtgatcagc cagaaaaaa cttgatcat tacaagaac 
      ttaaacgct ttggaaaat agcgatgtc atgcctgaa 
      gggtgggac aaagctaaa cctttcgaa agtctccct 
      gggcctaaa cccatacca ttttttggg aacatatgg 
      cgatttttg gttccgaaa attggagat ttttacgga 
      atcgatttt ttggacttg cacaaaatt ttttacgaa 
      cagtatgga gatatttcg attcttaaa ggcatgatt 
      aacgacccg attgtggtg atatttaac ccaaaagac 
      tacgaaacg ttgttcaga aatgaaggc atttggccg 
      atcagaaaa ggccttcag tcttttaac gagtatcgc 
      aaatcgagg aaagacatc ttcgtgaac gcaggatta 
      cttctcgaa gaaggagaa aactggttc aagataaga 
      tcagtagtt aatccgatt ttgatgcag ccgagaaat 
      atctcccag tacacggac aaaatgaat tcagtcgct 
      gacgaactt gttaacaat attacaaat ttgttggaa 
      gagagcgaa aacggggaa atgccggaa aatttccac 
      aatgaactg tacaaatgg tctctggag tcggtcggg 
      cttgtaacc ttagacaca cacttaggg tgtttgaag 
      aatgacttg gacgaagac tcagaaccg caaaaactg 
      atacggagc actttggaa atgttcaaa ttgatgcac 
      aaactcgat gtcttgcct tcatttcac aattacgtc 
      agtacgccg tcttggagg aaattcgtc caagtgatg 
      gactttata gtcgagaca aatatgaaa tatgtaaac 
      caagtttta gacaaatta gaaaaaggc gaaactaca 
      agtaccgag ataccgagt gttttggaa aagttgcta 
      aaagttgac cgcaacatc gccgtgacc atgtccata 
      gacatgatg atcgcagga atagacacg accggtcga 
      attttagga gcggctttg tatttcttg ggcaaaaac 
      cacgaagct caggaaaaa ctacggtcc gaagccatt 
      tcgctgctt caaactaaa gatcaggcg gtaacgagc 
      gaggtttta aaaaaggct ccttacctc aaagctgtg 
      attaaagaa acgacgcgt ttggcgcct atcggaatt 
      ggaaatttg cgaacgact gttaaaaat ttagttttg 
      gggggatat cagattcca aaggggacc gatgtggtt 
      acaagtaat ttagttttg tgcacaaac gacgagtat 
      ttttcgagg gctaaggag tttatcccc gagcgatgg 
      ctttccacg acttcggga gagttgtcg aagaaaaac 
      acaaatccg tttattttt gctccgttt ggttacggg 
      ccaaggagt tgtgtgggg aaaagactg gctagtttg 
      gagctagaa gtggcgctt ctcaaaatt ataagaaac 
      tttgagttg gattggcca catgaagat atggttttt 
      aaaaccaaa atgttgtac gggatgacg gagcctttg 
      aaaattcat gtcaggtct gtctaa
    mRNA sequence
    • >TC016365 RA
      ttttggaaa acaaatctc ccaaacagt cacaggttc 
      cggtcggtc tggggtgtt ttcaactaa ataattggc 
      aatttggca aaaaggtga gacgaaaat tgaataaat 
      tgcattgcc tttaacctt cagagcata ttagttaat 
      aaaaaccga ccatcacag tcatacatt attagttgc 
      gacgaatat cagcacgaa aaattataa tttgaggcg 
      ctaaaatgt taaaaacga agttactga agtttgcca 
      acacttcgc aaaagattt tgatgtcaa cgggcagtg 
      aaaatgtga tcagccaga aaaaacttg atcattaca 
      agaacttaa acgctttgg aaaatagcg atgtcatgc 
      ctgaagggt gggacaaag ctaaacctt tcgaaagtc 
      tccctgggc ctaaaccca taccatttt ttgggaaca 
      tatggcgat ttttggttc cgaaaattg gagattttt 
      acggaatcg attttttgg acttgcaca aaatttttt 
      acgaacagt atggagata tttcgattc ttaaaggca 
      tgattaacg acccgattg tggtgatat ttaacccaa 
      aagactacg aaacgttgt tcagaaatg aaggcattt 
      ggccgatca gaaaaggcc ttcagtctt ttaacgagt 
      atcgcaaat cgaggaaag acatcttcg tgaacgcag 
      gattacttc tcgaagaag gagaaaact ggttcaaga 
      taagatcag tagttaatc cgattttga tgcagccga 
      gaaatatct cccagtaca cggacaaaa tgaattcag 
      tcgctgacg aacttgtta acaatatta caaatttgt 
      tggaagaga gcgaaaacg gggaaatgc cggaaaatt 
      tccacaatg aactgtaca aatggtctc tggagtcgg 
      tcgggcttg taaccttag acacacact tagggtgtt 
      tgaagaatg acttggacg aagactcag aaccgcaaa 
      aactgatac ggagcactt tggaaatgt tcaaattga 
      tgcacaaac tcgatgtct tgccttcat ttcacaatt 
      acgtcagta cgccgtctt ggaggaaat tcgtccaag 
      tgatggact ttatagtcg agacaaata tgaaatatg 
      taaaccaag ttttagaca aattagaaa aaggcgaaa 
      ctacaagta ccgagatac cgagtgttt tggaaaagt 
      tgctaaaag ttgaccgca acatcgccg tgaccatgt 
      ccatagaca tgatgatcg caggaatag acacgaccg 
      gtcgaattt taggagcgg ctttgtatt tcttgggca 
      aaaaccacg aagctcagg aaaaactac ggtccgaag 
      ccatttcgc tgcttcaaa ctaaagatc aggcggtaa 
      cgagcgagg ttttaaaaa aggctcctt acctcaaag 
      ctgtgatta aagaaacga cgcgtttgg cgcctatcg 
      gaattggaa atttgcgaa cgactgtta aaaatttag 
      ttttggggg gatatcaga ttccaaagg ggaccgatg 
      tggttacaa gtaatttag ttttgtgca caaacgacg 
      agtattttt cgagggcta aggagttta tccccgagc 
      gatggcttt ccacgactt cgggagagt tgtcgaaga 
      aaaacacaa atccgttta tttttgctc cgtttggtt 
      acgggccaa ggagttgtg tggggaaaa gactggcta 
      gtttggagc tagaagtgg cgcttctca aaattataa 
      gaaactttg agttggatt ggccacatg aagatatgg 
      tttttaaaa ccaaaatgt tgtacggga tgacggagc 
      ctttgaaaa ttcatgtca ggtctgtct aatgaaaaa 
      caccaaaca aacaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY