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Gene information
General information
TC009730 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4984798..4988267
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009730
    iBeetle screen iB_07319

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009730 RA
      MTTYRTSSG KQQKFDWDK FWQYLKPHI WYLIAAIAG 
      ALAVAILNI QIPQVMGGV INVIAQYTD NKNSESFIN 
      EMKVPAIKL IAMYVGQSL CTFFYIYML SNLGERISF 
      QMKTDLFAA IMRQDIAFF DQQRTGEVI NRLTSDIQD 
      FKSSFKQTV SGGLRAIAQ IVGCSVSLI MISPHMTFI 
      TLLCVPTVI AVGTLFGGL LRNTSRKAQ AQIEKTTAV 
      ADEAVSNIR TVRAFGMED QERELFDAE AELAMVLNE 
      NLGFGIGWF QAGTNLFLN GMVLITLYM GGYLLSTDK 
      LSPGELMAY LMASQTIQR SLGQLSLLF GSVVRGVAA 
      GSRVFEYMN LRPTMPLRG GKIIPEDNL KGDIEFKNV 
      TFAYPTRRQ QYVLKNFDL KIPSGKTLA IVGASGNGK 
      STVVALIER FYDVNCGSV TLDGHDIKT LDPSWLRQR 
      VLGLISQEP ILFGTSIVE NIRYGLPSA SDEEVKEAA 
      KLANAHDFI SSFPQGYDT MVGERGVTL SGGQKQRIA 
      IARALLKNP TVLLLDEAT SALDTESEK VVQNALENA 
      KKGRTVVVI AHRLSTVQN ADIIVVLNK GVIVEMGTH 
      EELTRKRGY YWSLTYQQQ EVPAG
    CDS sequence
    • >TC009730 RA
      atgacaaca tacaggaca tcctctggc aaacagcaa 
      aaatttgat tgggataaa ttttggcag tatttaaaa 
      ccccatatt tggtatctt attgcggca attgctgga 
      gctttagct gttgcgatc ttaaatata caaataccg 
      caagtaatg ggaggggtt attaatgtt atagctcag 
      tatacagac aacaagaat agtgaatca tttataaat 
      gaaatgaaa gtgcctgcg attaaactt attgcaatg 
      tatgttggc caatcactt tgtacgttt ttttacatt 
      tacatgtta tctaatttg ggtgaaaga atttcattt 
      caaatgaaa accgattta tttgctgca ataatgcgc 
      caagatatt gcgttcttt gaccaacaa agaaccggt 
      gaggtgatc aacagactg acttccgat atccaagac 
      tttaaaagc agttttaaa cagacggta tcgggcggt 
      ttaagggca attgcccaa attgtggga tgctcagtg 
      tccctaatt atgatttca cctcacatg acttttatt 
      acgttactt tgtgtcccc acagttatt gctgttggg 
      acgcttttt gggggcttg ttaaggaac acttcaagg 
      aaagcacag gcacagatt gaaaaaaca accgctgtc 
      gcggatgaa gctgtaagc aacataaga acagtccga 
      gcgtttggg atggaagat caagagagg gaattattt 
      gatgctgag gctgaatta gcaatggtt ttaaatgaa 
      aatttgggt tttggtatt gggtggttc caagcgggt 
      acaaattta tttttaaat gggatggtt ttaattaca 
      ctttacatg ggtggttat ttgctctca actgataag 
      ttgtctccg ggagaattg atggcgtat ttgatggcc 
      tcccaaact attcagagg tcattgggg caactgtcc 
      ctgcttttt gggtccgtg gtcaggggg gttgcggca 
      ggatcgcgt gtttttgag tatatgaat ttacgacca 
      actatgcct ttaagagga gggaaaata attccagaa 
      gataatctg aagggtgat attgagttc aaaaacgta 
      acatttgct tatccaacg cgacgacaa cagtatgtt 
      ttaaagaat tttgattta aaaatacct tctggtaaa 
      actttggca atagttgga gcttcaggt aacggcaaa 
      tctacggtt gttgcgctg atagaacga ttttacgac 
      gtaaattgc ggttcggtc actttagac ggtcatgac 
      attaaaacc ctcgaccca tcatggttg agacagcgt 
      gttctgggg ctaataagc caagagcca attttgttt 
      ggtacttcc attgtcgaa aatatcaga tatgggctc 
      cctagtgcc tcagatgag gaagtcaaa gaagcggca 
      aaattagca aacgcccat gactttatc tccagtttt 
      ccgcaaggg tatgacact atggtgggt gagagaggg 
      gtgacatta tccggtggc caaaaacaa agaattgcc 
      attgctaga gcgttactt aaaaacccg acagtatta 
      ctattagat gaagcaaca agtgcttta gacacagaa 
      tcggagaaa gtcgtacaa aacgcatta gaaaatgct 
      aaaaagggg aggaccgtt gtggtgatc gcccaccga 
      ctgtccacg gttcagaac gctgacata attgttgtt 
      ttgaataag ggggttata gttgagatg gggacccat 
      gaggagttg acacgcaag cggggctat tactggtct 
      ctgacgtac caacagcag gaggttccc gccggttaa
    mRNA sequence
    • >TC009730 RA
      tgtcaaatg acaaaatat ctactttca ataggttta 
      tttttgtga taaattagt cattgtaaa tcaattttt 
      gataaatag gatgtgaag cagtattgc agaatgtgg 
      aaattaatg cagagcgcc tctaagctg tcttccagc 
      ttaagaaat tatgtattg agcaagaac tgtgccttt 
      cattaccgg aactaccaa agtagtaag acattattt 
      aatgatgct gcgaaagaa tttgttaaa aaaacagat 
      aaaaaacct aattttaat ttttcaaca tgtacaaag 
      atagcattg gggttatca agtggtggt attttgcaa 
      attgctttg gtaaacaac agggcttta tgtacagct 
      aaaaccaat agaatgaca acatacagg acatcctct 
      ggcaaacag caaaaattt gattgggat aaattttgg 
      cagtattta aaaccccat atttggtat cttattgcg 
      gcaattgct ggagcttta gctgttgcg atcttaaat 
      atacaaata ccgcaagta atgggaggg gttattaat 
      gttatagct cagtataca gacaacaag aatagtgaa 
      tcatttata aatgaaatg aaagtgcct gcgattaaa 
      cttattgca atgtatgtt ggccaatca ctttgtacg 
      tttttttac atttacatg ttatctaat ttgggtgaa 
      agaatttca tttcaaatg aaaaccgat ttatttgct 
      gcaataatg cgccaagat attgcgttc tttgaccaa 
      caaagaacc ggtgaggtg atcaacaga ctgacttcc 
      gatatccaa gactttaaa agcagtttt aaacagacg 
      gtatcgggc ggtttaagg gcaattgcc caaattgtg 
      ggatgctca gtgtcccta attatgatt tcacctcac 
      atgactttt attacgtta ctttgtgtc cccacagtt 
      attgctgtt gggacgctt tttgggggc ttgttaagg 
      aacacttca aggaaagca caggcacag attgaaaaa 
      acaaccgct gtcgcggat gaagctgta agcaacata 
      agaacagtc cgagcgttt gggatggaa gatcaagag 
      agggaatta tttgatgct gaggctgaa ttagcaatg 
      gttttaaat gaaaatttg ggttttggt attgggtgg 
      ttccaagcg ggtacaaat ttattttta aatgggatg 
      gttttaatt acactttac atgggtggt tatttgctc 
      tcaactgat aagttgtct ccgggagaa ttgatggcg 
      tatttgatg gcctcccaa actattcag aggtcattg 
      gggcaactg tccctgctt tttgggtcc gtggtcagg 
      ggggttgcg gcaggatcg cgtgttttt gagtatatg 
      aatttacga ccaactatg cctttaaga ggagggaaa 
      ataattcca gaagataat ctgaagggt gatattgag 
      ttcaaaaac gtaacattt gcttatcca acgcgacga 
      caacagtat gttttaaag aattttgat ttaaaaata 
      ccttctggt aaaactttg gcaatagtt ggagcttca 
      ggtaacggc aaatctacg gttgttgcg ctgatagaa 
      cgattttac gacgtaaat tgcggttcg gtcacttta 
      gacggtcat gacattaaa accctcgac ccatcatgg 
      ttgagacag cgtgttctg gggctaata agccaagag 
      ccaattttg tttggtact tccattgtc gaaaatatc 
      agatatggg ctccctagt gcctcagat gaggaagtc 
      aaagaagcg gcaaaatta gcaaacgcc catgacttt 
      atctccagt tttccgcaa gggtatgac actatggtg 
      ggtgagaga ggggtgaca ttatccggt ggccaaaaa 
      caaagaatt gccattgct agagcgtta cttaaaaac 
      ccgacagta ttactatta gatgaagca acaagtgct 
      ttagacaca gaatcggag aaagtcgta caaaacgca 
      ttagaaaat gctaaaaag gggaggacc gttgtggtg 
      atcgcccac cgactgtcc acggttcag aacgctgac 
      ataattgtt gttttgaat aagggggtt atagttgag 
      atggggacc catgaggag ttgacacgc aagcggggc 
      tattactgg tctctgacg taccaacag caggaggtt 
      cccgccggt taatttgta ttttttgta tgaaataaa 
      ttgtttgtg ttgga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY