Status
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Gene information
General information
TC033102
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010460
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7599930..7601059
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033102
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07315

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033102 RA
      MGRTLPTPV PCRVCGDRS FGKHYGVYC CDGCSCFFK 
      RSVRRNIIY TCISGEGRC IVDKARRNW CPYCRLQRC 
      FSVQMNVAA VQEERGPRK PKLVSRKPS YSINSESFV 
      YSTVSRPLP PETNPIQEL AAQILLVAI KQARCNSGF 
      GLLNRASQN LILSHLWAP LFLLRAAHW PSESADLFP 
      GVKGTFRVV RQLKLNLTD LEIVENILL CRADILDDV 
      EQITLALNV LTRALDELA VRTVLERRR FADILLALP 
      VLFVPSAVV LHSLLFKPV IGAVPIETV ISTI
    CDS sequence
    • >TC033102 RA
      atggggcgg acgttgcca acgccggtg ccttgtcga 
      gtttgtggg gacagatct tttggcaaa cactatggg 
      gtttattgc tgtgatggg tgttcgtgt tttttcaag 
      aggagtgtc cgccggaac attatttac acctgtatt 
      tctggcgag ggtcgctgt attgttgat aaagcaaga 
      cgaaactgg tgtccctat tgccgatta caacgctgt 
      ttttccgtt caaatgaac gtagccgcc gtccaagag 
      gaacgtggg ccaagaaaa ccgaaacta gtctccaga 
      aagccttct tactcgata aattccgaa agttttgtc 
      tactctact gtatccaga ccccttcct ccggaaacc 
      aacccaatc caggaacta gccgcccaa attttgctc 
      gtcgctata aaacaagcc cgctgtaat tccggcttt 
      ggattactg aatcgtgct tcgcaaaac ttgattttg 
      agccacttg tgggcgcct ttatttctg ctaagggcg 
      gctcattgg ccctctgaa agcgctgac ttattccca 
      ggtgttaag ggcactttc agggttgtc agacagtta 
      aaactaaac ttaacggac ttggaaata gttgaaaat 
      attttgcta tgtagggcg gatattttg gatgacgtg 
      gaacagata acattggct ttgaacgtt ttgacgaga 
      gctttagac gaactggcg gttagaaca gtcttggag 
      agaagaaga tttgccgat attttgctc gctttgcct 
      gtgcttttt gtaccatcg gctgtagtg ttgcattct 
      ttgcttttt aagccggtt attggagct gttcctatc 
      gaaaccgta atttccacg atttag
    mRNA sequence
    • >TC033102 RA
      ttagaaaag aaatacatg gggcggacg ttgccaacg 
      ccggtgcct tgtcgagtt tgtggggac agatctttt 
      ggcaaacac tatggggtt tattgctgt gatgggtgt 
      tcgtgtttt ttcaagagg agtgtccgc cggaacatt 
      atttacacc tgtatttct ggcgagggt cgctgtatt 
      gttgataaa gcaagacga aactggtgt ccctattgc 
      cgattacaa cgctgtttt tccgttcaa atgaacgta 
      gccgccgtc caagaggaa cgtgggcca agaaaaccg 
      aaactagtc tccagaaag ccttcttac tcgataaat 
      tccgaaagt tttgtctac tctactgta tccagaccc 
      cttcctccg gaaaccaac ccaatccag gaactagcc 
      gcccaaatt ttgctcgtc gctataaaa caagcccgc 
      tgtaattcc ggctttgga ttactgaat cgtgcttcg 
      caaaacttg attttgagc cacttgtgg gcgccttta 
      tttctgcta agggcggct cattggccc tctgaaagc 
      gctgactta ttcccaggt gttaagggc actttcagg 
      gttgtcaga cagttaaaa ctaaactta acggacttg 
      gaaatagtt gaaaatatt ttgctatgt agggcggat 
      attttggat gacgtggaa cagataaca ttggctttg 
      aacgttttg acgagagct ttagacgaa ctggcggtt 
      agaacagtc ttggagaga agaagattt gccgatatt 
      ttgctcgct ttgcctgtg ctttttgta ccatcggct 
      gtagtgttg cattctttg ctttttaag ccggttatt 
      ggagctgtt cctatcgaa accgtaatt tccacgatt 
      tagagaaat aaaaaaggg tgttagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY