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Gene information
General information
TC013670 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6444197..6446983
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013670
    iBeetle screen iB_07314

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    remarks: eggs lost, low validity due to technical reasons - should be repeated: egg number not scorable,
    Adult morphology
    remarks: eggs lost, low validity due to technical reasons - should be repeated: egg number not scorable,
    Analysis of embryonic musculature and early development
    remarks: eggs lost, (egglay)
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013670 RA
      MNNLNLVTV FTVLCCAAS FGHNQSARN FNSDCGQVF 
      TQKQFVIES PGYPSNYPP DFECSYLVK GPTCPTRFS 
      LQFVDFSIE ESLGCYKDR LEIGDKDAV CGSRYDTKE 
      YFSDKGTLI LKFVSDKDI TGRGYRIFV SRSSCQSEQ 
      PTIPSVPKW HTTNSDVEY TTTKRPTYN QGNKCCQTS 
      YNSKQFTLA SPNFPYSMS KPTECLFEI YKTNSNICR 
      LRIHVQFFW LGRPSNNCP EGFLEIDGK FICGCQRDL 
      KLIVPFGNE HTKILRFVS RGYERSSYS GFVMNFVQD 
      ECPKKYIPE DVKTNLTQE KFRFYNDQI KRVAWPNKS 
      HDSVIEKLH LISENTEVI DDKPQVVRH VYFYAPPDF 
      NRIPRDREE TDYIDTSIV STSFVQNSY SYNYCLNWN 
      RLQFNALPL RNFPICKTD EVSEGNVEP RKCLELNHL 
      RGYFKSPGY PFYYPGNMH ICYRFVKEP GYCSIKIHM 
      RDFNLQNSN ACENDYLLF ANQLRYCGS RLSNTISTL 
      DLRDKPYEE ITFVTDEFY CGRGFFGSY EQIPCQEKP 
      ITPAPIPCS RTIQDEMFT LDVYDYHES PCSFLIKKS 
      KQYVCSVTL YFERFDLVC ASESLVIDG TMYCGHLSG 
      QSVKISVTD QDPVIIYKR ISPAGVDGL RLRIKGVQT 
      SEDCPYPEV PAERIFHIP SPRKIQLEN NSKFLLKID 
      KHFKDICGV VTNTTEINL PETFCDFIM QNQKNITNC 
      LPIKANSLF VPIDSHKIE LKIQDVEKT EEIEFQEVD 
      CDEVGHMVE
    CDS sequence
    • >TC013670 RA
      atgaataac ttaaatctt gtgacagtt tttaccgtg 
      ttgtgttgt gctgcatct tttggccat aatcaaagt 
      gctcgtaat ttcaacagt gattgtggc caagtcttc 
      acccaaaaa caatttgta atagaatcg ccaggttac 
      ccgagcaat tatccccca gattttgaa tgctcgtac 
      ctcgtaaaa ggacccaca tgtcccact cgtttcagt 
      ctccagttt gttgacttc agcattgaa gaatcgtta 
      ggctgttat aaagaccgg ctggaaatc ggcgataaa 
      gatgccgtt tgcggttca agatacgac acgaaagag 
      tatttttct gataaaggc accctgatt ttaaaattt 
      gtctccgat aaagatata actggaagg ggatacaga 
      attttcgta tcacgctct tcatgtcag tcagagcaa 
      ccgactatt ccgtcagtc cccaaatgg catacaaca 
      aattctgat gtagaatac actaccact aaacggccc 
      acgtacaac caaggcaac aaatgttgc caaacttct 
      tacaactcg aaacagttt actttggca agccctaac 
      tttccctac agcatgagc aaacccaca gaatgtctg 
      tttgaaatt tataaaacc aatagcaac atttgtaga 
      ctaagaatt cacgttcag ttcttctgg ttgggaaga 
      cctagtaac aactgtccg gaaggtttt ttggaaatt 
      gatggcaaa tttatttgt ggttgccaa agagaccta 
      aaattaatc gtcccgttt ggaaatgag cacacaaag 
      attttgcga tttgtgagc agaggttac gaaagatcg 
      tcatatagc ggtttcgtc atgaatttt gtacaagat 
      gagtgccct aagaaatat atccctgaa gacgtgaaa 
      acaaaccta acgcaagaa aaatttagg ttttacaac 
      gatcaaatt aaacgcgtt gcttggcct aacaagtct 
      cacgattct gtgattgaa aagttgcac ttaattagc 
      gaaaacacg gaagtgatt gacgataaa ccgcaagtt 
      gtcagacac gtgtatttc tacgctcca cctgacttt 
      aaccgaatt cctcgagac cgtgaggag accgattac 
      atcgacacc tcaattgtt agcacctct tttgtgcaa 
      aatagttac agttacaac tattgtctt aactggaat 
      cgactgcaa tttaacgct ctacctttg aggaatttt 
      ccgatctgt aagacggac gaagtgagc gaaggtaat 
      gttgagccc agaaaatgc ctagaattg aaccacctt 
      cggggttac tttaaaagt cccggatat cccttttat 
      tatccaggc aatatgcac atttgttac aggtttgta 
      aaagagcca ggatattgc agcatcaaa atacacatg 
      agagacttc aaccttcag aacagcaat gcatgcgaa 
      aatgactat ttgctcttt gcaaaccaa ttgagatat 
      tgtgggagt cgcttatct aatacaatt tcgactctc 
      gatttgcgg gacaaaccc tacgaagag atcactttc 
      gtgacggac gaattttac tgtggtagg ggttttttc 
      ggtagttat gagcaaatt ccctgtcag gaaaaaccg 
      atcactcct gcgccaata ccttgctca aggacaatc 
      caggacgag atgtttact ctcgatgtt tatgattac 
      cacgagtct ccttgctcg tttctaata aagaaatcc 
      aaacagtat gtttgttcc gtaacactt tattttgag 
      aggtttgat ttggtttgt gcatctgag tcattagtc 
      attgatggg acgatgtat tgtggacat ttgagtgga 
      cagtctgtc aagatcagc gtcacagat caagaccct 
      gtaattatt tataagaga atttcacct gccggtgtc 
      gatgggctc aggttacgt attaaggga gtccagact 
      tcggaagat tgtccttac cctgaagtt cccgcagaa 
      agaattttc catattccg tcgccgcga aaaatacaa 
      ctcgaaaat aattcgaag tttcttttg aaaattgat 
      aaacatttt aaagatatt tgtggagta gtgaccaat 
      acaactgaa attaatctt cctgaaaca ttctgtgac 
      tttataatg caaaaccaa aaaaacatt acaaactgt 
      cttcctatt aaagcgaat tcgcttttt gtaccgatt 
      gactcgcat aaaattgag ctcaagatt caagatgtg 
      gaaaaaact gaagagatt gagtttcag gaagtcgac 
      tgcgatgaa gttgggcat atggtggag tag
    mRNA sequence
    • >TC013670 RA
      accacttaa gacatttaa cctttcata attgccatt 
      cataaaaac tcctcagat gcgttaaga ccttaactc 
      aaaatgaat aacttaaat cttgtgaca gtttttacc 
      gtgttgtgt tgtgctgca tcttttggc cataatcaa 
      agtgctcgt aatttcaac agtgattgt ggccaagtc 
      ttcacccaa aaacaattt gtaatagaa tcgccaggt 
      tacccgagc aattatccc ccagatttt gaatgctcg 
      tacctcgta aaaggaccc acatgtccc actcgtttc 
      agtctccag tttgttgac ttcagcatt gaagaatcg 
      ttaggctgt tataaagac cggctggaa atcggcgat 
      aaagatgcc gtttgcggt tcaagatac gacacgaaa 
      gagtatttt tctgataaa ggcaccctg attttaaaa 
      tttgtctcc gataaagat ataactgga aggggatac 
      agaattttc gtatcacgc tcttcatgt cagtcagag 
      caaccgact attccgtca gtccccaaa tggcataca 
      acaaattct gatgtagaa tacactacc actaaacgg 
      cccacgtac aaccaaggc aacaaatgt tgccaaact 
      tcttacaac tcgaaacag tttactttg gcaagccct 
      aactttccc tacagcatg agcaaaccc acagaatgt 
      ctgtttgaa atttataaa accaatagc aacatttgt 
      agactaaga attcacgtt cagttcttc tggttggga 
      agacctagt aacaactgt ccggaaggt tttttggaa 
      attgatggc aaatttatt tgtggttgc caaagagac 
      ctaaaatta atcgtcccg tttggaaat gagcacaca 
      aagattttg cgatttgtg agcagaggt tacgaaaga 
      tcgtcatat agcggtttc gtcatgaat tttgtacaa 
      gatgagtgc cctaagaaa tatatccct gaagacgtg 
      aaaacaaac ctaacgcaa gaaaaattt aggttttac 
      aacgatcaa attaaacgc gttgcttgg cctaacaag 
      tctcacgat tctgtgatt gaaaagttg cacttaatt 
      agcgaaaac acggaagtg attgacgat aaaccgcaa 
      gttgtcaga cacgtgtat ttctacgct ccacctgac 
      tttaaccga attcctcga gaccgtgag gagaccgat 
      tacatcgac acctcaatt gttagcacc tcttttgtg 
      caaaatagt tacagttac aactattgt cttaactgg 
      aatcgactg caatttaac gctctacct ttgaggaat 
      tttccgatc tgtaagacg gacgaagtg agcgaaggt 
      aatgttgag cccagaaaa tgcctagaa ttgaaccac 
      cttcggggt tactttaaa agtcccgga tatcccttt 
      tattatcca ggcaatatg cacatttgt tacaggttt 
      gtaaaagag ccaggatat tgcagcatc aaaatacac 
      atgagagac ttcaacctt cagaacagc aatgcatgc 
      gaaaatgac tatttgctc tttgcaaac caattgaga 
      tattgtggg agtcgctta tctaataca atttcgact 
      ctcgatttg cgggacaaa ccctacgaa gagatcact 
      ttcgtgacg gacgaattt tactgtggt aggggtttt 
      ttcggtagt tatgagcaa attccctgt caggaaaaa 
      ccgatcact cctgcgcca ataccttgc tcaaggaca 
      atccaggac gagatgttt actctcgat gtttatgat 
      taccacgag tctccttgc tcgtttcta ataaagaaa 
      tccaaacag tatgtttgt tccgtaaca ctttatttt 
      gagaggttt gatttggtt tgtgcatct gagtcatta 
      gtcattgat gggacgatg tattgtgga catttgagt 
      ggacagtct gtcaagatc agcgtcaca gatcaagac 
      cctgtaatt atttataag agaatttca cctgccggt 
      gtcgatggg ctcaggtta cgtattaag ggagtccag 
      acttcggaa gattgtcct taccctgaa gttcccgca 
      gaaagaatt ttccatatt ccgtcgccg cgaaaaata 
      caactcgaa aataattcg aagtttctt ttgaaaatt 
      gataaacat tttaaagat atttgtgga gtagtgacc 
      aatacaact gaaattaat cttcctgaa acattctgt 
      gactttata atgcaaaac caaaaaaac attacaaac 
      tgtcttcct attaaagcg aattcgctt tttgtaccg 
      attgactcg cataaaatt gagctcaag attcaagat 
      gtggaaaaa actgaagag attgagttt caggaagtc 
      gactgcgat gaagttggg catatggtg gagtagatt 
      tagttgaac attctgtat tttttactt tttatataa 
      taaataaat ggataaccc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY