Status
Please wait ...
Gene information
General information
TC008239 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7117235..7124361
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008239
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07313

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008239 RA
      MVILRLWTI FFVFFKFVF KSNGATHWV VTEKGMIQS 
      HVGSPLDLR RPYDLISLL DQEKRWNEV QTVLQELMT 
      RKSTIEEKW SNLQGNPSM ESKVALKNS DCLMNGKLL 
      NAIDIYNIA TNGSERVKV DLTIEKDDV TNYHVPDCS 
      KYSKIEFSL PDYERFPSL HSDDNITLV PEDSLEKLM 
      PVTSVRLFG HEIHKELVK NSSSWVHYN LGALFWRMK 
      GNGPKAVDC SRRALHYVP TIYRDIPLH NLAGILHKA 
      GRSKEATLM LHTALESAS KQNLHYLAL GNIYFAMGD 
      YNNSLLFYD KFLELEPEH NEVNVMKQA TLCFSKLET 
      HLIDIQENV LGYLQSILS DLHDFHSLQ QHWLRLSKR 
      LMWEHSSYD EELGNFQDD VFDISNFGK KSQRCVKKM 
      STISCDLVD SPIADFDTM NLQNLFQYV ESETQKINE 
      RMAKTSRLN TMKEGNNAP QNYPKFPTT MSTSGEKYF 
      DVAGWPSEE ECKKWDLPV SDKDDLKLP IFLPPENKG 
      YDINKILSD SIGLPNGSE HKLPWYPPV CDESNAFGE 
      KYVQQSERH LLNNEIKSN QFLRNHFVK YVNNGKADE 
      AEIGQRIIT AIEKKSAPS WILSTLASL YWRIRGNTR 
      KSLDCLDLA LKTAPKDQT DVILVSISS IVHQLGLVN 
      QALKYANLA FKLNYVEPS TNFLLALLH YESNPLMAM 
      YYMKNVLRV DPEYYDGQA ELLLKIWGC RVKMGTYNN 
      VKKPSEKLP QEICSEKES FKGQGMICS ANGDDCKTA 
      SIQCFHTKN LEGSGDVEP DQSQLERIS DSPHPFHMR 
      ILLGDDQVT PGPTPEFYV GFSDDSTSE TVLHVYDKS 
      GTYSLSSQG CKQIIEADW VHFTSMWQS IAARNLDIG 
      PYLKPLPKN AKKRSTKPY CSDASFSEG DTLLVQFTN 
      KILRSISTT SPDKSLAEW LGIMAGDQK ASVEELGAK 
      IGLALQENT TSWLLATAA AIYWRVVGN TEEAIVCLR 
      LALNHVPDN MKDVPLINL ANVLQRFGI NDDALDVAY 
      MALKSNPNF VVNHFTVGN ILAAMGDLE EAISFHRSA 
      LALDSNFEP ARNRLQAIL CTLLFDESG TLRNIPEN
    CDS sequence
    • >TC008239 RA
      atggtgata ttaaggttg tggacgatt ttttttgtg 
      tttttcaaa tttgttttt aaaagtaat ggggccacg 
      cattgggtt gtcaccgaa aagggcatg attcagtca 
      catgtgggg tccccgctg gatctccgc cgtccctac 
      gacttgatc tctctccta gaccaagaa aagcgttgg 
      aatgaggtc caaaccgtt ctccaagag ctcatgacg 
      cgtaaatcg accattgag gaaaaatgg tcaaacttg 
      caaggaaac cctagcatg gagtcaaaa gtggctctt 
      aaaaattct gattgtttg atgaacggc aaactttta 
      aacgccatt gatatttat aatatcgca accaacgga 
      agcgaacgc gtcaaagtc gatttaacg attgagaaa 
      gatgacgtt acgaattac cacgtccct gattgtagt 
      aaatacagc aaaattgag tttagttta ccggattat 
      gagcggttt ccgtctctg catagtgac gataatatc 
      actttggtt ccggaggac tctctcgag aagctgatg 
      ccggtgacg tcggtccgg ttgtttggt cacgagata 
      cataaagaa ttagtgaaa aattcgagt tcgtgggtg 
      cattataat ttaggagcg ttgttttgg aggatgaag 
      ggcaacggg cccaaggct gtggattgc agcagaagg 
      gcattgcat tatgtccct acaatctac agggatatc 
      ccgctacat aacttggca gggattttg cacaaagcc 
      gggcgttcg aaagaagca actttgatg cttcacact 
      gcactcgaa tctgcctcc aaacaaaac ttgcactat 
      cttgccttg ggcaacatt tactttgcc atgggcgat 
      tataacaat tcactttta ttttacgac aaattcctt 
      gaactcgaa cctgagcat aacgaagtt aacgtaatg 
      aaacaagca actctttgt ttctcaaaa ctcgaaact 
      cacttaatc gacattcaa gagaatgtt ttaggatac 
      cttcaaagt attttatcc gatttgcac gattttcac 
      agtttgcaa cagcactgg ttgaggttg agtaagcgg 
      ttgatgtgg gaacactcg tcctatgac gaggaattg 
      gggaatttt caggacgac gtgttcgat atttcaaat 
      ttcgggaaa aagagccaa cgctgcgtg aaaaaaatg 
      tccacgatc tcgtgcgat cttgtcgat agtccaatt 
      gcagacttc gacacgatg aatctacag aatttattt 
      cagtatgtc gagtcggaa actcaaaaa attaacgaa 
      cgtatggca aaaactagt cgtttaaat acgatgaaa 
      gagggtaac aatgccccg caaaattac ccaaaattt 
      cccacgaca atgtcaaca tcgggggag aaatacttc 
      gatgttgcc gggtggccg tcagaagaa gagtgcaaa 
      aagtgggac ttaccagtc agtgacaaa gacgattta 
      aaattgccc attttcttg ccgccggaa aataaaggc 
      tacgatata aataaaata ttgtcggac tcgatcggt 
      ttgcctaac gggtcagaa cataaatta ccttggtat 
      cctccagtt tgtgacgag tcgaacgct ttcggggaa 
      aaatacgtc cagcagtcg gagcggcat cttcttaac 
      aatgaaatt aaaagtaac cagtttttg cgaaatcat 
      tttgttaaa tatgttaat aatgggaaa gccgacgag 
      gctgagatc ggtcaaagg atcataact gcgattgaa 
      aagaaaagt gccccgagt tggattttg tcaactctt 
      gccagcctc tattggcga attcgtggc aacactcgc 
      aaatcgctc gattgtctc gacctagct ctaaaaacc 
      gccccaaag gaccagact gatgtgatt ctagtttcg 
      attagttcg atagtgcat caattaggc ttagtgaac 
      caagcattg aagtacgcg aatttggcg ttcaagctg 
      aattacgta gaaccaagt acaaatttt ttacttgca 
      cttttgcac tatgaaagc aacccgttg atggccatg 
      tattacatg aaaaatgtc ctacgggtc gacccggag 
      tattacgac ggtcaggcc gaattgttg ctcaaaata 
      tggggctgt cgcgtcaag atgggtact tacaataac 
      gtgaaaaaa ccgagcgag aagttgccg caggagatt 
      tgttcggaa aaagagtcg tttaagggg caggggatg 
      atctgttcg gcgaatggg gatgattgc aagacggcc 
      tccatccag tgttttcac acgaagaac ctcgaaggt 
      tccggtgat gttgaaccc gaccagtca cagctagaa 
      cgcattagt gattctcct caccctttt cacatgagg 
      attttgcta ggggatgac caagtcact cccggacct 
      acaccggaa ttttacgtc ggtttcagc gacgattca 
      acctctgaa accgttctc cacgtctat gacaaatcc 
      ggtacttat tctctgtct tcgcaaggg tgcaaacag 
      ataatcgaa gccgattgg gtccatttt acctcaatg 
      tggcaatca atcgctgcg cgaaactta gatatcggc 
      ccgtacttg aaaccttta cccaaaaac gcgaaaaaa 
      cgtagcacg aagccatac tgcagcgac gccagtttc 
      agtgaaggc gacacgctt ttggtgcag tttacgaac 
      aaaatccta cgtagcata tccactacc tcacctgat 
      aaaagttta gccgagtgg ctggggatc atggcaggg 
      gaccagaaa gcctccgtc gaggagttg ggcgcgaaa 
      atcgggttg gctttgcag gaaaacacc acctcgtgg 
      cttttggcc accgctgct gcgatttac tggcgggtg 
      gttggcaac acagaggag gctatcgtg tgtttacgg 
      ctggctctg aaccacgtg ccggacaat atgaaggat 
      gtgccgttg atcaacttg gcgaatgtt ttgcagagg 
      tttgggatc aacgacgac gcgctggac gtggcctat 
      atggcgctg aaaagcaac ccgaatttt gtggtgaat 
      cacttcacg gtgggtaat atcctggcg gccatgggc 
      gacttggag gaggcgatt tcgttccat cgatcggcg 
      ctcgccttg gattcgaat tttgaacca gcgaggaat 
      cgattgcag gcgatcttg tgcacgctg ctgttcgac 
      gagtcgggg actttgcgg aatatacct gagaattaa
    mRNA sequence
    • >TC008239 RA
      taccagacg agagctgtc atgcgttga attatcaca 
      acacataaa tgtcaaaag attcgtaaa aatggtgat 
      attaaggtt gtggacgat tttttttgt gtttttcaa 
      atttgtttt taaaagtaa tggggccac gcattgggt 
      tgtcaccga aaagggcat gattcagtc acatgtggg 
      gtccccgct ggatctccg ccgtcccta cgacttgat 
      ctctctcct agaccaaga aaagcgttg gaatgaggt 
      ccaaaccgt tctccaaga gctcatgac gcgtaaatc 
      gaccattga ggaaaaatg gtcaaactt gcaaggaaa 
      ccctagcat ggagtcaaa agtggctct taaaaattc 
      tgattgttt gatgaacgg caaactttt aaacgccat 
      tgatattta taatatcgc aaccaacgg aagcgaacg 
      cgtcaaagt cgatttaac gattgagaa agatgacgt 
      tacgaatta ccacgtccc tgattgtag taaatacag 
      caaaattga gtttagttt accggatta tgagcggtt 
      tccgtctct gcatagtga cgataatat cactttggt 
      tccggagga ctctctcga gaagctgat gccggtgac 
      gtcggtccg gttgtttgg tcacgagat acataaaga 
      attagtgaa aaattcgag ttcgtgggt gcattataa 
      tttaggagc gttgttttg gaggatgaa gggcaacgg 
      gcccaaggc tgtggattg cagcagaag ggcattgca 
      ttatgtccc tacaatcta cagggatat cccgctaca 
      taacttggc agggatttt gcacaaagc cgggcgttc 
      gaaagaagc aactttgat gcttcacac tgcactcga 
      atctgcctc caaacaaaa cttgcacta tcttgcctt 
      gggcaacat ttactttgc catgggcga ttataacaa 
      ttcactttt attttacga caaattcct tgaactcga 
      acctgagca taacgaagt taacgtaat gaaacaagc 
      aactctttg tttctcaaa actcgaaac tcacttaat 
      cgacattca agagaatgt tttaggata ccttcaaag 
      tattttatc cgatttgca cgattttca cagtttgca 
      acagcactg gttgaggtt gagtaagcg gttgatgtg 
      ggaacactc gtcctatga cgaggaatt ggggaattt 
      tcaggacga cgtgttcga tatttcaaa tttcgggaa 
      aaagagcca acgctgcgt gaaaaaaat gtccacgat 
      ctcgtgcga tcttgtcga tagtccaat tgcagactt 
      cgacacgat gaatctaca gaatttatt tcagtatgt 
      cgagtcgga aactcaaaa aattaacga acgtatggc 
      aaaaactag tcgtttaaa tacgatgaa agagggtaa 
      caatgcccc gcaaaatta cccaaaatt tcccacgac 
      aatgtcaac atcggggga gaaatactt cgatgttgc 
      cgggtggcc gtcagaaga agagtgcaa aaagtggga 
      cttaccagt cagtgacaa agacgattt aaaattgcc 
      cattttctt gccgccgga aaataaagg ctacgatat 
      aaataaaat attgtcgga ctcgatcgg tttgcctaa 
      cgggtcaga acataaatt accttggta tcctccagt 
      ttgtgacga gtcgaacgc tttcgggga aaaatacgt 
      ccagcagtc ggagcggca tcttcttaa caatgaaat 
      taaaagtaa ccagttttt gcgaaatca ttttgttaa 
      atatgttaa taatgggaa agccgacga ggctgagat 
      cggtcaaag gatcataac tgcgattga aaagaaaag 
      tgccccgag ttggatttt gtcaactct tgccagcct 
      ctattggcg aattcgtgg caacactcg caaatcgct 
      cgattgtct cgacctagc tctaaaaac cgccccaaa 
      ggaccagac tgatgtgat tctagtttc gattagttc 
      gatagtgca tcaattagg cttagtgaa ccaagcatt 
      gaagtacgc gaatttggc gttcaagct gaattacgt 
      agaaccaag tacaaattt tttacttgc acttttgca 
      ctatgaaag caacccgtt gatggccat gtattacat 
      gaaaaatgt cctacgggt cgacccgga gtattacga 
      cggtcaggc cgaattgtt gctcaaaat atggggctg 
      tcgcgtcaa gatgggtac ttacaataa cgtgaaaaa 
      accgagcga gaagttgcc gcaggagat ttgttcgga 
      aaaagagtc gtttaaggg gcaggggat gatctgttc 
      ggcgaatgg ggatgattg caagacggc ctccatcca 
      gtgttttca cacgaagaa cctcgaagg ttccggtga 
      tgttgaacc cgaccagtc acagctaga acgcattag 
      tgattctcc tcacccttt tcacatgag gattttgct 
      aggggatga ccaagtcac tcccggacc tacaccgga 
      attttacgt cggtttcag cgacgattc aacctctga 
      aaccgttct ccacgtcta tgacaaatc cggtactta 
      ttctctgtc ttcgcaagg gtgcaaaca gataatcga 
      agccgattg ggtccattt tacctcaat gtggcaatc 
      aatcgctgc gcgaaactt agatatcgg cccgtactt 
      gaaaccttt acccaaaaa cgcgaaaaa acgtagcac 
      gaagccata ctgcagcga cgccagttt cagtgaagg 
      cgacacgct tttggtgca gtttacgaa caaaatcct 
      acgtagcat atccactac ctcacctga taaaagttt 
      agccgagtg gctggggat catggcagg ggaccagaa 
      agcctccgt cgaggagtt gggcgcgaa aatcgggtt 
      ggctttgca ggaaaacac cacctcgtg gcttttggc 
      caccgctgc tgcgattta ctggcgggt ggttggcaa 
      cacagagga ggctatcgt gtgtttacg gctggctct 
      gaaccacgt gccggacaa tatgaagga tgtgccgtt 
      gatcaactt ggcgaatgt tttgcagag gtttgggat 
      caacgacga cgcgctgga cgtggccta tatggcgct 
      gaaaagcaa cccgaattt tgtggtgaa tcacttcac 
      ggtgggtaa tatcctggc ggccatggg cgacttgga 
      ggaggcgat ttcgttcca tcgatcggc gctcgcctt 
      ggattcgaa ttttgaacc agcgaggaa tcgattgca 
      ggcgatctt gtgcacgct gctgttcga cgagtcggg 
      gactttgcg gaatatacc tgagaatta aatatattt 
      cacgaaatt ataaaagtt ctatttttg catgcccta 
      cggagtata tgtgttgtc ctttcgaga aactagatt 
      ggatctaaa ttgtttatc gagttgatt aggtgtcta 
      ggttggttc tttcaacaa tttgtgtga tcaattgtc 
      acaagtgat atttttaca ttttttatt ttagtactt 
      aatttgcca aagatttgg aactgactt gtgttcact 
      attctagaa gtaggtttt tgcagacta cattccagc 
      gattatttg tgacttaat tagtttttg gtatgattt 
      caatacttg cagtgacaa acattacga atctcctgt 
      ttttgtgaa atattgtta tcactgttt aattaaacg 
      atttttaaa caa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY