Status
Please wait ...
Gene information
General information
TC032819
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008124,TC008123
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5334546..5339126
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032819
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07312

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032819 RA
      MNPVIAKNI LLFLFITQF YLEETFASV LFNKLSTYK 
      KDGSNHKDL EGAFSELTA DQPFVKASY DKFNEDKYA 
      TICWNERTT LHIFKNGTN YGYTEYSIS LEASTQLAP 
      NKSETSGAE VYEITDNDT KKSRQLVFV TVQSTELET 
      IKQNLTVMC RSGKGFSKS FIDYPERKR VEIKLKPLF 
      QNLESGTSS KYNIHSSKH SIEIKTSLD PSDSKGPLA 
      PRMFSPTLN NMQDSNSKL KATEGVGPR MMSTSDASK 
      TAENAAKSP QDSDITMLP RMLFTNKNK QEISSSKDS 
      ASTPDPESK ISPRILPGN GNLQPKKTQ DNAASSQQK 
      NTVTSPQVP SQNQDLNKS KTVKVESAT KVTDKTKSN 
      IDVSKPASS SVLQKTEIK SSPAKISKP ESEKIKESS 
      KSSETKITH RILPTVENA QQTKSPHLE STKTIESLP 
      SLEVKTSPK NSPKSQNLQ QSTSQAKPE KTTLSSNPA 
      PVVKNPEAK QEEINSKSA PTTQSEEKT QNSQQKEMT 
      HPAAQAPKT VPQNQDLQV KTAKVDGVE PKKISKNDA 
      EKNKSNANT TKDSQTNKS SSISKLATS PKIRTKVIG 
      PTIIPISLR METEKKNLH REKSNIALL PAEKTDPAA 
      KTKPSEASA ENSAKLPDQ NKDLEQGKP NATPPTKLD 
      LYSIFPKSL QDYITSIKN KITNKNSPQ TNNSQNSAK 
      DNQSTKTSE KDNTQDNLN TKTSEIDNT QMSLRMMPS 
      DYEDEVAHI ISVPNNYKE LEIEDQLSD HMIEDPSFQ 
      NFYAQHSSS FEQTGEEGN VGTQLVNCK NGKVTVKLN 
      MDILKGNSK VKNIAKEIQ FDINHPLEA KNRHFDDQL 
      RALVRNYLG DDLYLKNCV GSENSPLEI EAMPRSLQA 
      NQKEEATAV NLPKPFQNS GVPQARHSD PDEDIDYET 
      RPFYAISPY SPVKTFYVD ETGVYHVVP YNHQPNQPS 
      LLQRLSNNL KTVFRKLTP TDSYSYILV TFLSVLCFT 
      IAQDYNCTT AGRFRYRDP TCRSYYLCV LYNGAYVKS 
      DYTCPSTMV FDPTSQACS GTAVCIDNI CSTQPLIPL 
      FPKLPDPNG PDCRTYIQC MGLVDKYPV IGTCPSGLL 
      FDQDFSPQA DCWIQGNCT
    CDS sequence
    • >TC032819 RA
      atgaatcca gtaattgcg aaaaatatt ttgttgttc 
      ctgtttata acgcagttt tatttggaa gaaacattt 
      gcctcagtt ttatttaat aagttgtcc acttacaaa 
      aaagacgga agcaatcac aaggatttg gaaggagca 
      ttttctgaa ttaactgcg gatcagccc tttgttaaa 
      gcgtcttat gacaaattt aatgaagac aaatatgcc 
      accatttgc tggaatgag agaactact ttgcatatt 
      ttcaagaat ggcaccaat tatggttac actgagtac 
      tcaattagc ttggaagct tcgacacaa ttagcacca 
      aataaatct gaaacttct ggagcggaa gtttatgaa 
      atcacggac aatgacaca aaaaaatct cgccaatta 
      gtttttgta acagtgcaa agtaccgaa ctcgagaca 
      attaagcaa aatctcact gtaatgtgc aggtccggt 
      aaaggtttt tctaaatca tttattgat tatcctgaa 
      agaaaacga gtggaaatt aagctgaaa ccacttttt 
      caaaatctt gaaagtggg acaagtagc aaatataac 
      atacattca agcaaacac tcgattgaa ataaaaacc 
      tcattagac ccatcagac tctaaagga cctcttgca 
      ccacgaatg ttttctccg actttaaac aatatgcaa 
      gattcaaac tctaaatta aaagctacc gaaggagtt 
      ggaccaaga atgatgtct acaagtgac gcaagcaag 
      actgctgaa aacgctgcg aagtcgcca caagattca 
      gacatcaca atgttaccc agaatgtta tttacgaat 
      aaaaataag caggaaatt tcaagctcg aaggattcc 
      gcatctact ccagaccca gaatcaaaa atatcacca 
      agaattttg cctggcaat ggaaatttg caaccaaaa 
      aaaacacaa gacaatgca gcaagctct caacaaaaa 
      aatacagtt acatcccca caagttcct tcacaaaat 
      caggattta aataaatcg aaaacagtt aaagttgaa 
      agtgctact aaagtcact gataaaacc aaatcaaac 
      atagacgtt tccaaacct gcatcaagt tcagtttta 
      caaaaaact gagataaag tcttcaccc gcaaaaatt 
      tctaagcca gaatctgaa aaaattaaa gaatcttct 
      aaaagttct gagacaaag ataacacac agaatttta 
      cctacggtt gaaaatgcg caacaaaca aaatctcct 
      catctagaa tctacaaag acaatagag tctctaccg 
      agtttagag gtcaaaacg tcaccaaaa aattcaccc 
      aagagccag aatctacaa cagtcaact tctcaggct 
      aaacctgaa aaaactacc cttagttcc aatcctgcg 
      ccagtagtt aaaaatcca gaagcgaaa caagaagag 
      ataaactca aaatcggct ccaactact cagtccgaa 
      gaaaagaca caaaattct caacaaaaa gaaatgact 
      catccagct gcacaagca ccaaaaact gtccctcaa 
      aatcaagat ttgcaagta aaaacagca aaagtagat 
      ggagtagag cccaaaaaa atctctaaa aacgatgct 
      gagaaaaat aaatcaaat gcaaacaca acaaaagat 
      tcacagacc aacaaatct tcatcaatt tctaagctt 
      gcaacatca ccaaaaata cgaacaaaa gtaatcggt 
      ccaacaata ataccaata tcacttaga atggaaact 
      gagaaaaag aatttgcat cgagaaaaa tcaaacata 
      gcattgtta ccagctgag aaaactgat ccagcagca 
      aaaacgaaa ccttcagaa gcttctgca gaaaattct 
      gcaaaattg cctgatcag aataaagat ttggagcaa 
      ggaaagcca aacgctaca cctcccacg aaactggat 
      ttgtattca atttttcct aaatcgctt caagattat 
      ataacaagt attaaaaat aagataact aacaaaaat 
      tcgcctcaa accaacaac tcccaaaat tctgctaaa 
      gacaatcaa agtacgaaa acttctgaa aaagacaac 
      acacaagac aatttaaat acgaaaact tctgaaata 
      gataacaca caaatgtcg ttacgaatg atgccgtca 
      gactacgaa gatgaagtt gcccacatt atttcagtc 
      ccaaacaac tacaaggag ttggaaatt gaagaccaa 
      ctaagtgat cacatgatt gaagatccc agctttcaa 
      aatttttat gcacaacac agttcaagt ttcgaacaa 
      actggtgaa gaaggaaat gtcgggacg caacttgtg 
      aattgtaaa aacggaaag gtgaccgtc aagttaaac 
      atggacata ctgaaagga aattcgaaa gtgaaaaac 
      attgcgaag gaaattcag tttgacatt aaccaccct 
      ctcgaagcc aaaaacaga cactttgac gatcagttg 
      agagctttg gttcgtaat tatttaggc gatgattta 
      tatctgaaa aactgcgtt gggagtgaa aattctcca 
      ctagagatt gaagccatg ccacgttct ttgcaagcc 
      aaccaaaaa gaggaagcc acggcggta aatttacca 
      aaaccgttc caaaatagt ggagtacca caagccagg 
      cattcagac ccggatgaa gacattgat tatgaaact 
      agaccattt tacgccata tctccctac agcccagta 
      aaaactttc tacgtggac gaaaccggt gtgtaccat 
      gtggtaccc tacaaccac caacccaac caaccttca 
      ttgctccaa cgtttatca aataatctc aaaacagtc 
      ttccgtaaa ttaactccg acagatagc tatagttat 
      attttagtc acatttctg tcggttttg tgctttaca 
      atcgcacag gattataac tgtaccacc gctggcagg 
      ttcaggtac agggatcca acttgtcgt tcctattat 
      ttatgcgtc ttgtacaat ggggcgtac gttaaaagc 
      gactatact tgtcccagt accatggtt tttgaccca 
      acgagtcaa gcatgctcc ggtaccgcc gtctgtatc 
      gacaacatt tgctccacc caacccttg attcccctg 
      tttcccaaa ctaccagac cccaacggt cccgactgt 
      cgcacttac atccagtgc atgggcctg gttgacaaa 
      tatcccgtg atcgggacc tgtcccagt ggcctcctc 
      ttcgaccag gatttcagc ccacaggca gattgctgg 
      atacaagga aattgcaca taa
    mRNA sequence
    • >TC032819 RA
      agactgtgg taaagatga atccagtaa ttgcgaaaa 
      atattttgt tgttcctgt ttataacgc agttttatt 
      tggaagaaa catttgcct cagttttat ttaataagt 
      tgtccactt acaaaaaag acggaagca atcacaagg 
      atttggaag gagcatttt ctgaattaa ctgcggatc 
      agccctttg ttaaagcgt cttatgaca aatttaatg 
      aagacaaat atgccacca tttgctgga atgagagaa 
      ctactttgc atattttca agaatggca ccaattatg 
      gttacactg agtactcaa ttagcttgg aagcttcga 
      cacaattag caccaaata aatctgaaa cttctggag 
      cggaagttt atgaaatca cggacaatg acacaaaaa 
      aatctcgcc aattagttt ttgtaacag tgcaaagta 
      ccgaactcg agacaatta agcaaaatc tcactgtaa 
      tgtgcaggt ccggtaaag gtttttcta aatcattta 
      ttgattatc ctgaaagaa aacgagtgg aaattaagc 
      tgaaaccac tttttcaaa atcttgaaa gtgggacaa 
      gtagcaaat ataacatac attcaagca aacactcga 
      ttgaaataa aaacctcat tagacccat cagactcta 
      aaggacctc ttgcaccac gaatgtttt ctccgactt 
      taaacaata tgcaagatt caaactcta aattaaaag 
      ctaccgaag gagttggac caagaatga tgtctacaa 
      gtgacgcaa gcaagactg ctgaaaacg ctgcgaagt 
      cgccacaag attcagaca tcacaatgt tacccagaa 
      tgttattta cgaataaaa ataagcagg aaatttcaa 
      gctcgaagg attccgcat ctactccag acccagaat 
      caaaaatat caccaagaa ttttgcctg gcaatggaa 
      atttgcaac caaaaaaaa cacaagaca atgcagcaa 
      gctctcaac aaaaaaata cagttacat ccccacaag 
      ttccttcac aaaatcagg atttaaata aatcgaaaa 
      cagttaaag ttgaaagtg ctactaaag tcactgata 
      aaaccaaat caaacatag acgtttcca aacctgcat 
      caagttcag ttttacaaa aaactgaga taaagtctt 
      cacccgcaa aaatttcta agccagaat ctgaaaaaa 
      ttaaagaat cttctaaaa gttctgaga caaagataa 
      cacacagaa ttttaccta cggttgaaa atgcgcaac 
      aaacaaaat ctcctcatc tagaatcta caaagacaa 
      tagagtctc taccgagtt tagaggtca aaacgtcac 
      caaaaaatt cacccaaga gccagaatc tacaacagt 
      caacttctc aggctaaac ctgaaaaaa ctaccctta 
      gttccaatc ctgcgccag tagttaaaa atccagaag 
      cgaaacaag aagagataa actcaaaat cggctccaa 
      ctactcagt ccgaagaaa agacacaaa attctcaac 
      aaaaagaaa tgactcatc cagctgcac aagcaccaa 
      aaactgtcc ctcaaaatc aagatttgc aagtaaaaa 
      cagcaaaag tagatggag tagagccca aaaaaatct 
      ctaaaaacg atgctgaga aaaataaat caaatgcaa 
      acacaacaa aagattcac agaccaaca aatcttcat 
      caatttcta agcttgcaa catcaccaa aaatacgaa 
      caaaagtaa tcggtccaa caataatac caatatcac 
      ttagaatgg aaactgaga aaaagaatt tgcatcgag 
      aaaaatcaa acatagcat tgttaccag ctgagaaaa 
      ctgatccag cagcaaaaa cgaaacctt cagaagctt 
      ctgcagaaa attctgcaa aattgcctg atcagaata 
      aagatttgg agcaaggaa agccaaacg ctacacctc 
      ccacgaaac tggatttgt attcaattt ttcctaaat 
      cgcttcaag attatataa caagtatta aaaataaga 
      taactaaca aaaattcgc ctcaaacca acaactccc 
      aaaattctg ctaaagaca atcaaagta cgaaaactt 
      ctgaaaaag acaacacac aagacaatt taaatacga 
      aaacttctg aaatagata acacacaaa tgtcgttac 
      gaatgatgc cgtcagact acgaagatg aagttgccc 
      acattattt cagtcccaa acaactaca aggagttgg 
      aaattgaag accaactaa gtgatcaca tgattgaag 
      atcccagct ttcaaaatt tttatgcac aacacagtt 
      caagtttcg aacaaactg gtgaagaag gaaatgtcg 
      ggacgcaac ttgtgaatt gtaaaaacg gaaaggtga 
      ccgtcaagt taaacatgg acatactga aaggaaatt 
      cgaaagtga aaaacattg cgaaggaaa ttcagtttg 
      acattaacc accctctcg aagccaaaa acagacact 
      ttgacgatc agttgagag ctttggttc gtaattatt 
      taggcgatg atttatatc tgaaaaact gcgttggga 
      gtgaaaatt ctccactag agattgaag ccatgccac 
      gttctttgc aagccaacc aaaaagagg aagccacgg 
      cggtaaatt taccaaaac cgttccaaa atagtggag 
      taccacaag ccaggcatt cagacccgg atgaagaca 
      ttgattatg aaactagac cattttacg ccatatctc 
      cctacagcc cagtaaaaa ctttctacg tggacgaaa 
      ccggtgtgt accatgtgg taccctaca accaccaac 
      ccaaccaac cttcattgc tccaacgtt tatcaaata 
      atctcaaaa cagtcttcc gtaaattaa ctccgacag 
      atagctata gttatattt tagtcacat ttctgtcgg 
      ttttgtgct ttacaatcg cacaggatt ataactgta 
      ccaccgctg gcaggttca ggtacaggg atccaactt 
      gtcgttcct attatttat gcgtcttgt acaatgggg 
      cgtacgtta aaagcgact atacttgtc ccagtacca 
      tggtttttg acccaacga gtcaagcat gctccggta 
      ccgccgtct gtatcgaca acatttgct ccacccaac 
      ccttgattc ccctgtttc ccaaactac cagacccca 
      acggtcccg actgtcgca cttacatcc agtgcatgg 
      gcctggttg acaaatatc ccgtgatcg ggacctgtc 
      ccagtggcc tcctcttcg accaggatt tcagcccac 
      aggcagatt gctggatac aaggaaatt gcacataag 
      aaaccttgt cctttgcaa taaataaat aaaagtgtt 
      ttaagaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY