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Gene information
General information
TC007456 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4264559..4266160
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007456
    iBeetle screen iB_07311

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50
    53
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007456 RA
      MSRRVCRLC LASPSVCYS LLEDNLSEM LEALTSIKV 
      DSTDVSPIS CLKCLLNMK LAFNIQQKF LRSDEKLKE 
      FAILNNVEV KLEVEEELL LEMNSINVG AETDSCPSS 
      CIVCGNKVD EKLLAKHIQ QHFDSAQIC DTCDQSFKN 
      IAEYKSHIH VNHPQTSHK CLHCGASFR YKSLYNVHL 
      SQAHKTCRK RPKREASLG PFTCKECEK QFTCKRKFN 
      SHTHRRKKC PICGAQITI SNFRKHTLN HSSGPQICE 
      LCGATLSNL ESLRGHLTY THSSQIFNC DKCGKVFHK 
      RYTYQLHLK KHAGEKSHV CETCGKAFF TMYYLNKHV 
      KTAHLKLRP YICQFCKKA FSSRFALRT HERQHTNET 
      PYKCEVCGE GFRQNVSLR AHRKSKHNI IEPKNCACP 
      VCGKLFGSE QAILSHMRL H
    CDS sequence
    • >TC007456 RA
      atgagtaga cgggtatgt aggttatgt ttagcctcc 
      ccctcagtc tgctattcg ctgttagaa gacaacctt 
      tccgaaatg ttagaagct ttaacttcc attaaggtt 
      gattctact gatgtatca ccgataagt tgcttgaaa 
      tgtttgcta aatatgaaa ctagcattc aatatccaa 
      caaaaattt ttaagatct gacgaaaag ttgaaagaa 
      tttgcaatt ttaaataac gttgaagtg aaattagaa 
      gtcgaagag gagttgtta ctagaaatg aattcaatt 
      aatgttggg gctgaaaca gactcatgt ccttcctct 
      tgtatagtt tgtggtaac aaagtggac gaaaaactt 
      cttgcaaaa catattcag caacacttc gacagtgcg 
      caaatttgc gatacttgt gaccaatca ttcaaaaat 
      attgcagaa tacaaaagc cacatccac gtgaatcac 
      ccccagacc agtcacaag tgtctccac tgtggcgca 
      agtttccgg tacaagagt ctatacaac gttcatttg 
      agccaagcg cacaaaaca tgtagaaaa cgaccgaaa 
      agagaagcc tcactgggg ccctttaca tgcaaagaa 
      tgtgagaaa caattcacc tgtaaacgc aaatttaac 
      agtcacacc catcggcgc aaaaagtgc cccatttgc 
      ggggcgcaa atcaccatt agcaatttc cgcaagcac 
      accttgaac cattcgtcg gggccccaa atttgcgag 
      ctttgcggg gcgactttg tccaatttg gagagtctg 
      agagggcat ctcacgtac acgcatagt tcgcagatt 
      ttcaactgt gacaagtgc ggtaaagtg tttcacaaa 
      cggtacacc taccagctc cacttgaaa aaacacgcg 
      ggggagaag tcccacgtg tgtgagacc tgcggcaag 
      gcttttttc acaatgtac tatttgaat aaacacgtg 
      aaaacggcg cacttgaaa ctgcgtccg tacatttgc 
      cagttttgc aaaaaagcg ttttcgagt aggtttgcc 
      ttgaggacg catgagagg cagcatact aacgagacg 
      ccgtacaag tgcgaggtt tgcggggag ggcttccgg 
      caaaatgtg tctctgaga gcgcacaga aagtccaaa 
      cacaacata attgaaccg aaaaattgc gcttgcccg 
      gtctgtgga aagctgttc gggtcggaa caggccata 
      ctcagccac atgaggcta cactaa
    mRNA sequence
    • >TC007456 RA
      tgttcactg aaattgata atttggtct aactattac 
      ccaaaagga gaaatgaga ctaaaaaat tttaaattc 
      cacgtgaaa atgtttcaa aaaggattg ttcatcaag 
      tcgcgttgg taccactgt tcaaagttt ttgatggtt 
      ttgttacta atcgatgtt aataaacaa tttaagatt 
      acgaataat taataaaat taattattg aaagtactc 
      ataaatcat tactgcatt aattggacg gcttacttt 
      gtaattaca gtcagaaat gagtagacg ggtatgtag 
      gttatgttt agcctcccc ctcagtctg ctattcgct 
      gttagaaga caacctttc cgaaatgtt agaagcttt 
      aacttccat taaggttga ttctactga tgtatcacc 
      gataagttg cttgaaatg tttgctaaa tatgaaact 
      agcattcaa tatccaaca aaaattttt aagatctga 
      cgaaaagtt gaaagaatt tgcaatttt aaataacgt 
      tgaagtgaa attagaagt cgaagagga gttgttact 
      agaaatgaa ttcaattaa tgttggggc tgaaacaga 
      ctcatgtcc ttcctcttg tatagtttg tggtaacaa 
      agtggacga aaaacttct tgcaaaaca tattcagca 
      acacttcga cagtgcgca aatttgcga tacttgtga 
      ccaatcatt caaaaatat tgcagaata caaaagcca 
      catccacgt gaatcaccc ccagaccag tcacaagtg 
      tctccactg tggcgcaag tttccggta caagagtct 
      atacaacgt tcatttgag ccaagcgca caaaacatg 
      tagaaaacg accgaaaag agaagcctc actggggcc 
      ctttacatg caaagaatg tgagaaaca attcacctg 
      taaacgcaa atttaacag tcacaccca tcggcgcaa 
      aaagtgccc catttgcgg ggcgcaaat caccattag 
      caatttccg caagcacac cttgaacca ttcgtcggg 
      gccccaaat ttgcgagct ttgcggggc gactttgtc 
      caatttgga gagtctgag agggcatct cacgtacac 
      gcatagttc gcagatttt caactgtga caagtgcgg 
      taaagtgtt tcacaaacg gtacaccta ccagctcca 
      cttgaaaaa acacgcggg ggagaagtc ccacgtgtg 
      tgagacctg cggcaaggc ttttttcac aatgtacta 
      tttgaataa acacgtgaa aacggcgca cttgaaact 
      gcgtccgta catttgcca gttttgcaa aaaagcgtt 
      ttcgagtag gtttgcctt gaggacgca tgagaggca 
      gcatactaa cgagacgcc gtacaagtg cgaggtttg 
      cggggaggg cttccggca aaatgtgtc tctgagagc 
      gcacagaaa gtccaaaca caacataat tgaaccgaa 
      aaattgcgc ttgcccggt ctgtggaaa gctgttcgg 
      gtcggaaca ggccatact cagccacat gaggctaca 
      ctaaagtta ttgtttcgg tgtaacaat aaaattaat 
      tcattgtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY