Status
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Gene information
General information
TC007721 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 234879..248021
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007721
    iBeetle screen iB_07309

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007721 RA
      MNSTNKHEF CEEFPHNFI PTPGYWIEC SRQKITSDT 
      IWDESESDH EIQEGDEED HTDGTDQEN GHASKRRDR 
      SCNDLYEDD EEDDSSSPR KKTTIEEQW EKNQGFELT 
      SVEQETYEK YFYGTEHWN YFTNDEDLG PVILSIKQE 
      TLNSRDQFR ILVRAISYT VHGLIPASC VFADRYNRE 
      EVVRSLGKE VNINPPLTL GQLPDTPEE LLKLDQVFI 
      KSELKVGVI YVKEGQYTE EEILDNNDN SVLFEEFLQ 
      LFGEKVRLK GFDKYKGGL DTVHDLTGL YSVYTNWRN 
      IEIMFHVST LLPYEKHDP QKLQRKRHI GNDIVCVVF 
      LEADNTSFS PACIKSHFL HTFILVRTS PRIKRKPTR 
      YEVSVVTRD EVGAYKPYL WEQSVFDKG PMFREWLLT 
      KIVNGERAS YSAPKFARM QERTRSQML EDIVANLQN 
      HAETGQIPK PYRRGSWRP IGHMRPSSP LLDSVRDQF 
      EDYDQIAKD FTRMFLNTE SNCLANAQL FDVTFLVGQ 
      SKQKAKLVG VRAILGVRS RVFQEMLYG IQTGFGSPQ 
      VPVAELLAR PVPTLLSPQ QSRPKSSNF LQVPDIESP 
      RPKSVPSSP MVKRAFSRL GTITAGWGR SIRKHNAQQ 
      LSAEDKKKW ASSQDCSNK DGKDKEGKD KPSAAQLPV 
      PRLSVCADA QKVDRAKLA QTEFSIIEF DPDTFRILL 
      DYLHTGSCP LTCSTIPGL ICAAEHYDL PELLQACFH 
      HAKQFLRIE VVCCMLCSL ENYYWRYTS ASELVNMIL 
      AFVETRTYA LFTTPEFLT LSESMVQMI MCRNLEVPE 
      VRKFEAMLA WARHKIRTK TSSKLDAKL EFKCIMERL 
      ARDLKLYRI SPQELIKVV LPSKAIKNE RILETLMFQ 
      ANSGMYRIQ DSYLKECTQ RLQKQDSRF SEWESFDYS 
      T
    CDS sequence
    • >TC007721 RA
      atgaattcc acaaacaaa catgaattc tgcgaagaa 
      ttcccacac aacttcata ccaacgccc ggctattgg 
      atcgaatgc tccagacaa aaaatcacc tctgacacc 
      atatgggac gaaagcgaa tccgaccat gaaatacag 
      gagggtgac gaggaggac catactgat gggaccgac 
      caggaaaat ggacacgcg tccaagcga cgcgatcgc 
      agttgtaac gatttgtac gaagacgac gaagaggac 
      gactcgtca tccccgcgc aaaaaaacc acaatagag 
      gaacaatgg gagaaaaac caaggtttc gagcttacc 
      agcgtggag caggaaacc tacgagaaa tatttctat 
      ggtacagaa cactggaac tatttcacc aacgatgaa 
      gacctaggc cctgttatt ctatcgatt aagcaagaa 
      acgctaaat tcccgggat caatttagg atattagta 
      agagcaatc agttacacg gtccacggt ctgatccca 
      gccagttgt gtctttgcc gatcgttac aatcgtgaa 
      gaagttgtg agatcgttg ggtaaagaa gttaacata 
      aatccgccc ctaaccctt ggacaactc ccagatacg 
      cccgaagaa ctcttaaaa ttagatcaa gttttcata 
      aaatcggag cttaaagtg ggtgttatc tacgtaaaa 
      gagggtcaa tacaccgaa gaggagatt ctagacaat 
      aacgacaat tcagtcctg tttgaagag tttctgcag 
      cttttcggt gagaaggtg cgtctaaag ggcttcgat 
      aaatacaag ggcggtttg gatacagtg cacgattta 
      acaggatta tattcggtt tacacaaac tggcgaaac 
      attgaaatc atgtttcac gtcagcact ttactcccc 
      tacgaaaag cacgaccca caaaagctg cagaggaag 
      agacacatt ggcaacgat atcgtctgt gttgtgttt 
      ctggaagcc gataacacg tcgttctca ccggcttgt 
      atcaaaagc cactttttg cacaccttt attttagtg 
      cgtacttcc ccgagaatt aagaggaag cccactcga 
      tatgaagtg tcagtcgtt acaagagat gaagtgggt 
      gcctacaag ccttatcta tgggagcaa agtgttttc 
      gacaaggga ccgatgttc agggagtgg cttctcacg 
      aaaattgtc aacggggaa agggcaagt tattctgca 
      ccgaaattt gcaaggatg caagagagg acaaggtcg 
      caaatgctt gaggatatt gtggccaat ctgcaaaac 
      cacgcagag accggacaa atacccaag ccttacaga 
      agaggttcg tggcgcccc attggccac atgcgcccc 
      tcgtccccc ttgctggac agcgtccgc gaccagttc 
      gaagactac gaccaaatc gccaaagac ttcacccgc 
      atgttcctc aacacggaa tcaaactgt ttagcaaac 
      gcccaatta ttcgatgtg acctttctt gtcggccaa 
      tcgaaacaa aaagcgaaa ttagttggt gtgcgtgca 
      attttgggt gttcgtagt cgagtcttc caggagatg 
      ttgtacggc attcagact ggtttcggt tcgccgcaa 
      gtgcccgta gctgaacta ttagccagg ccagttccc 
      actctatta agcccccaa caaagtaga ccgaaaagt 
      agcaacttt ctacaagtg ccagacatt gaatcgcca 
      cgacccaaa agtgtcccc agctcgcca atggtcaaa 
      agggccttc tccaggctg ggcaccatc acagcgggc 
      tggggcagg tccattagg aaacacaac gcccaacaa 
      ctttccgct gaggataag aagaagtgg gccagcagt 
      caggactgc tcaaataaa gacggtaag gacaaggag 
      ggtaaggac aagccatca gcagcccaa ctgcccgtc 
      cctcgcttg agcgtttgc gctgatgct caaaaagtc 
      gatcgagcc aaactggca cagactgaa ttttctata 
      atcgaattc gatccagac acattccgg atattgtta 
      gattattta catactggc tcttgtccc ctcacatgt 
      tcgacaatt ccgggctta atttgcgca gctgaacac 
      tacgacctt cctgaactc ctccaagct tgcttccat 
      cacgccaaa caatttctc cgaatcgaa gtcgtgtgt 
      tgtatgtta tgctcattg gaaaactac tactggagg 
      tacacttca gcgtccgag ttggtcaac atgatcctg 
      gcatttgtc gaaacgcga acttatgcc cttttcacc 
      actccggag tttctaacg ctgagcgag tcaatggtt 
      cagatgatc atgtgcagg aatttggaa gttcccgaa 
      gttcggaag tttgaagct atgttagct tgggcgaga 
      cacaagatc cgaactaaa acttcaagt aaactcgat 
      gccaagtta gaatttaag tgcattatg gaaagattg 
      gcccgcgac ttgaaactg taccggatc tcgccgcag 
      gaactcatc aaggtggtg ctgccgtcg aaggcgatc 
      aagaacgag cgcatcttg gagacgctg atgttccag 
      gcgaattcg gggatgtac cgcatccag gactcgtat 
      ttgaaagag tgcacccaa agactgcag aaacaagac 
      tcccgattt tcggaatgg gagtcgttt gattacagt 
      acttaa
    mRNA sequence
    • >TC007721 RA
      caactaaat atttaaata aataaatta cgtttcaaa 
      tgaattcca caaacaaac atgaattct gcgaagaat 
      tcccacaca acttcatac caacgcccg gctattgga 
      tcgaatgct ccagacaaa aaatcacct ctgacacca 
      tatgggacg aaagcgaat ccgaccatg aaatacagg 
      agggtgacg aggaggacc atactgatg ggaccgacc 
      aggaaaatg gacacgcgt ccaagcgac gcgatcgca 
      gttgtaacg atttgtacg aagacgacg aagaggacg 
      actcgtcat ccccgcgca aaaaaacca caatagagg 
      aacaatggg agaaaaacc aaggtttcg agcttacca 
      gcgtggagc aggaaacct acgagaaat atttctatg 
      gtacagaac actggaact atttcacca acgatgaag 
      acctaggcc ctgttattc tatcgatta agcaagaaa 
      cgctaaatt cccgggatc aatttagga tattagtaa 
      gagcaatca gttacacgg tccacggtc tgatcccag 
      ccagttgtg tctttgccg atcgttaca atcgtgaag 
      aagttgtga gatcgttgg gtaaagaag ttaacataa 
      atccgcccc taacccttg gacaactcc cagatacgc 
      ccgaagaac tcttaaaat tagatcaag ttttcataa 
      aatcggagc ttaaagtgg gtgttatct acgtaaaag 
      agggtcaat acaccgaag aggagattc tagacaata 
      acgacaatt cagtcctgt ttgaagagt ttctgcagc 
      ttttcggtg agaaggtgc gtctaaagg gcttcgata 
      aatacaagg gcggtttgg atacagtgc acgatttaa 
      caggattat attcggttt acacaaact ggcgaaaca 
      ttgaaatca tgtttcacg tcagcactt tactcccct 
      acgaaaagc acgacccac aaaagctgc agaggaaga 
      gacacattg gcaacgata tcgtctgtg ttgtgtttc 
      tggaagccg ataacacgt cgttctcac cggcttgta 
      tcaaaagcc actttttgc acaccttta ttttagtgc 
      gtacttccc cgagaatta agaggaagc ccactcgat 
      atgaagtgt cagtcgtta caagagatg aagtgggtg 
      cctacaagc cttatctat gggagcaaa gtgttttcg 
      acaagggac cgatgttca gggagtggc ttctcacga 
      aaattgtca acggggaaa gggcaagtt attctgcac 
      cgaaatttg caaggatgc aagagagga caaggtcgc 
      aaatgcttg aggatattg tggccaatc tgcaaaacc 
      acgcagaga ccggacaaa tacccaagc cttacagaa 
      gaggttcgt ggcgcccca ttggccaca tgcgcccct 
      cgtccccct tgctggaca gcgtccgcg accagttcg 
      aagactacg accaaatcg ccaaagact tcacccgca 
      tgttcctca acacggaat caaactgtt tagcaaacg 
      cccaattat tcgatgtga cctttcttg tcggccaat 
      cgaaacaaa aagcgaaat tagttggtg tgcgtgcaa 
      ttttgggtg ttcgtagtc gagtcttcc aggagatgt 
      tgtacggca ttcagactg gtttcggtt cgccgcaag 
      tgcccgtag ctgaactat tagccaggc cagttccca 
      ctctattaa gcccccaac aaagtagac cgaaaagta 
      gcaactttc tacaagtgc cagacattg aatcgccac 
      gacccaaaa gtgtcccca gctcgccaa tggtcaaaa 
      gggccttct ccaggctgg gcaccatca cagcgggct 
      ggggcaggt ccattagga aacacaacg cccaacaac 
      tttccgctg aggataaga agaagtggg ccagcagtc 
      aggactgct caaataaag acggtaagg acaaggagg 
      gtaaggaca agccatcag cagcccaac tgcccgtcc 
      ctcgcttga gcgtttgcg ctgatgctc aaaaagtcg 
      atcgagcca aactggcac agactgaat tttctataa 
      tcgaattcg atccagaca cattccgga tattgttag 
      attatttac atactggct cttgtcccc tcacatgtt 
      cgacaattc cgggcttaa tttgcgcag ctgaacact 
      acgaccttc ctgaactcc tccaagctt gcttccatc 
      acgccaaac aatttctcc gaatcgaag tcgtgtgtt 
      gtatgttat gctcattgg aaaactact actggaggt 
      acacttcag cgtccgagt tggtcaaca tgatcctgg 
      catttgtcg aaacgcgaa cttatgccc ttttcacca 
      ctccggagt ttctaacgc tgagcgagt caatggttc 
      agatgatca tgtgcagga atttggaag ttcccgaag 
      ttcggaagt ttgaagcta tgttagctt gggcgagac 
      acaagatcc gaactaaaa cttcaagta aactcgatg 
      ccaagttag aatttaagt gcattatgg aaagattgg 
      cccgcgact tgaaactgt accggatct cgccgcagg 
      aactcatca aggtggtgc tgccgtcga aggcgatca 
      agaacgagc gcatcttgg agacgctga tgttccagg 
      cgaattcgg ggatgtacc gcatccagg actcgtatt 
      tgaaagagt gcacccaaa gactgcaga aacaagact 
      cccgatttt cggaatggg agtcgtttg attacagta 
      cttaagact tttttgtga cgaaaccct tagattaga 
      agacaatca atttctgca tccatcatt cgcgcggcg 
      ttgatatta tgtaacaat aataaatac caccacaca 
      cacacacaa aaatgtaaa gccatgcca accgggaac 
      aagtgattc atgttttat aatatgggt gtgattgca 
      agacaatct attatgaat taagtgtct gccttgcgt 
      gtacgattt tttattctt acaagcagt ctcggtcaa 
      tgtttactg ttggtttcg tgaatcgga atttccttg 
      tttaaatta atgtgagtc tccaattat gcgccttta 
      taacgatga ttatatgat gaacattat tttcaacaa 
      actgttaac tgtatttat aggaaacgt gtatctaat 
      acaagatct gtttttttc gtacctgtg ttgtgaaga 
      atactcggc aggttacgt ggaccctgt ctgtataga 
      gacttgttg ctgtttgta ttcgtgttc gatgtttca 
      tatttgaaa aacacacac aaaccaggt gtttgtaag 
      actaaatat attttaatt actcttaga tagcaaaaa 
      aatgttgaa tattttgat actaaataa ctaaagaag 
      aaaaaaaat cataaatgt ttctttacg caaatctgt 
      tcaatattt tatttgaca aataactaa taagtgtct 
      tattacaaa atagcagag caaatgaaa ctaagtact 
      aagtatatt taacatcgt tgtcaaatg ttgtctgtt 
      ttaacaaaa cttgatttt acttattgt tacctatcg 
      cgaaatacg acttgtagc cgccatcat tcactgaca 
      ctgttcaaa acattcaag ccaacaact attattaca 
      gcgaaaatg ctcaaacta atcacagac aagtcgtat 
      ttcctcgtt atgttcatt caacggagt gatgattcg 
      ttctgtttg cttgtactt gcgttgaaa accaaagaa 
      gtgtaataa ccaataatg atctaaata gaaacacca 
      atatttgtg aatgataat tttgttgta aagattgtt 
      gctttatca atgtacaag tgaatagaa tatttcaca 
      tattctaag atattggaa atcttattt tctcaaaat 
      acatttttt tgaagcgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY