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Gene information
General information
TC000394 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12120521..12151907
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000394
    iBeetle screen iB_07305

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000394 RA
      MWSSLLDPN GVIRERSGG VPIWHRGSE SSVQCGSRV 
      PRWSVGMPP SAAETLRAW LLSALVVHG AVAGNPDAK 
      RLYDDLLSN YNKLVRPVV NTSDVLRVC IKLKLSQLI 
      DVNLKNQIM TTNLWVEQS WYDYKLRWE PKEYGGVHM 
      LHVPSDHIW RPDIVLYNN ADGNYEVTL MTKATVYYN 
      GLVVWQPPA VYKSSCAID VEFFPYDVQ TCVLKLGSW 
      TYDGFKVDL RHMDEKAGS NVVDVGVDL SEFYMSVEW 
      DILEVPAVR NEKFYTCCD EPYLDITFN ITMRRKTLF 
      YTVNIIIPC MGISFLTVL TFYLPSDSG EKVTLSISI 
      LISLHVFFL LVVEIIPPT SLVVPLLGK YLIFAMILV 
      SISICVTVV VLNVHFRSP QTHKMSPWV KRVFIHILP 
      RLLVMKRPQ YVFETNSRF LMRGREPST CLYPYHTTV 
      QRDDCSPKR RYPTRTPSK EDLSPSLED GGPFGGSCQ 
      IHGPVAIPH SESEDLTVS AEADNGIKS PVLRNPGFS 
      HSRCPPEIH KSCFCVRFI AEHTKMLED STKVKEDWK 
      YVAMVLDRL FLWIFTLAV LVGTAGIIL QAPTLYDDR 
      IPIDKKFEE YTTTTVVRC PPPQ
    • >TC000394 RB
      MWSSLLDPN GVIRERSGG VPIWHRGSE SSVQCGSRV 
      PRWSVGMPP SAAETLRAW LLSALVVHG AVAGNPDAK 
      RLYDDLLSN YNKLVRPVV NTSDVLRVC IKLKLSQLI 
      DVNLKNQIM TTNLWVEQS WYDYKLRWE PKEYGGVHM 
      LHVPSDHIW RPDIVLYNN ADGNFEVTL ATKATIYHQ 
      GLVEWKPPA IYKSSCEID VEYFPFDEQ TCVLKFGSW 
      TYDGFKVDL RHMDEKAGS NVVDVGVDL SEFYMSVEW 
      DILEVPAVR NEKFYTCCD EPYLDITFN ITMRRKTLF 
      YTVNIIIPC MGISFLTVL TFYLPSDSG EKVTLSISI 
      LISLHVFFL LVVEIIPPT SLVVPLLGK YLIFAMILV 
      SISICVTVV VLNVHFRSP QTHKMSPWV KRVFIHILP 
      RLLVMKRPQ YVFETNSRF LMRGREPST CLYPYHTTV 
      QRDDCSPKR RYPTRTPSK EDLSPSLED GGPFGGSCQ 
      IHGPVAIPH SESEDLTVS AEADNGIKS PVLRNPGFS 
      HSRCPPEIH KSCFCVRFI AEHTKMLED STKVKEDWK 
      YVAMVLDRL FLWIFTLAV LVGTAGIIL QAPTLYDDR 
      IPIDKKFEE YTTTTVVRC PPPQ
    • >TC000394 RC
      MWSSLLDPN GVIRERSGG VPIWHRGSE SSVQCGSRV 
      PRWSVGMPP SAAETLRAW LLSALVVHG AVAGNPDAK 
      RLYDDLLSN YNKLVRPVV NTSDVLRVC IKLKLSQLI 
      DVNLKNQIM TTNLWVEQS WYDYKLRWE PKEYGGVHM 
      LHVPSDHIW RPDIVLYNN ADGNFEVTL ATKATIYHQ 
      GLVEWKPPA IYKSSCEID VEYFPFDEQ TCVLKFGSW 
      TYDGFKVFW VCVSGCGVS GNFGGCPAP RCVKAPGGN 
      GVDRWQPPP GCDADGNYE VTLMTKATV YYNGLVVWQ 
      PPAVYKSSC AIDVEFFPY DVQTCVLKL GSWTYDGFK 
      VDLRHMDEK AGSNVVDVG VDLSEFYMS VEWDILEVP 
      AVRNEKFYT CCDEPYLDI TFNITMRRK TLFYTVNII 
      IPCMGISFL TVLTFYLPS DSGEKVTLS ISILISLHV 
      FFLLVVEII PPTSLVVPL LGKYLIFAM ILVSISICV 
      TVVVLNVHF RSPQTHKMS PWVKRVFIH ILPRLLVMK 
      RPQYVFETN SRFLMRGRE PSTCLYPYH TTVQRDDCS 
      PKRRYPTRT PSKEDLSPS LEDGGPFGG SCQIHGPVA 
      IPHSESEDL TVSAEADNG IKSPVLRNP GFSHSRCPP 
      EIHKSCFCV RFIAEHTKM LEDSTKVKE DWKYVAMVL 
      DRLFLWIFT LAVLVGTAG IILQAPTLY DDRIPIDKK 
      FEEYTTTTV VRCPPPQ
    • >TC000394 RD
      MWSSLLDPN GVIRERSGG VPIWHRGSE SSVQCGSRV 
      PRWSVGMPP SAAETLRAW LLSALVVHG AVAGNPDAK 
      RLYDDLLSN YNKLVRPVV NTSDVLRVC IKLKLSQLI 
      DVNLKNQIM TTNLWVEQS WYDYKLRWE PKEYGGVHM 
      LHVPSDHIW RPDIVLYNN ADGNFEVTL ATKATIYHQ 
      GLVEWKPPA IYKSSCEID VEYFPFDEQ TCVLKFGSW 
      TYDGFKTPL THIDELRKG AAGSKHMIS NNTALKRAW 
      ADNRAAPQI YDHHMAITA DGNYEVTLM TKATVYYNG 
      LVVWQPPAV YKSSCAIDV EFFPYDVQT CVLKLGSWT 
      YDGFKVDLR HMDEKAGSN VVDVGVDLS EFYMSVEWD 
      ILEVPAVRN EKFYTCCDE PYLDITFNI TMRRKTLFY 
      TVNIIIPCM GISFLTVLT FYLPSDSGE KVTLSISIL 
      ISLHVFFLL VVEIIPPTS LVVPLLGKY LIFAMILVS 
      ISICVTVVV LNVHFRSPQ THKMSPWVK RVFIHILPR 
      LLVMKRPQY VFETNSRFL MRGREPSTC LYPYHTTVQ 
      RDDCSPKRR YPTRTPSKE DLSPSLEDG GPFGGSCQI 
      HGPVAIPHS ESEDLTVSA EADNGIKSP VLRNPGFSH 
      SRCPPEIHK SCFCVRFIA EHTKMLEDS TKVKEDWKY 
      VAMVLDRLF LWIFTLAVL VGTAGIILQ APTLYDDRI 
      PIDKKFEEY TTTTVVRCP PPQ
    CDS sequence
    • >TC000394 RA
      atgtggagt agtctgttg gatccgaat ggcgttatt 
      cgcgaacgg tcgggcggg gtgccgatc tggcatcgt 
      ggttccgaa tcgagcgtg cagtgtggc tccagagtg 
      ccgcggtgg agcgtcggg atgcctcct tctgccgcc 
      gagacgctg agagcctgg ctgctgtcg gccttggta 
      gtgcacggt gctgtggct ggaaatcct gatgcgaag 
      cgtctctac gatgacctg ctttccaac tacaacaag 
      ttagtgcgg cctgtggtc aacacctct gacgtcttg 
      cgagtttgt atcaaactc aaactttca caactcatc 
      gacgtgaac ttaaagaat caaataatg acaacaaat 
      ttgtgggtc gagcaatcg tggtacgac tacaaatta 
      aggtgggag ccgaaagaa tatgggggg gtgcacatg 
      ctccacgtt ccttcggat catatttgg aggccggac 
      atcgtcctt tacaataac gccgacggc aactatgag 
      gtcacattg atgaccaaa gccaccgtc tactacaac 
      ggcctcgtg gtgtggcag ccgcccgcg gtttacaag 
      tcctcgtgc gctatagac gtagaattc tttccgtac 
      gacgtccaa acatgcgtt ttgaagtta ggaagttgg 
      acgtacgat gggtttaag gtggacctg cgacacatg 
      gacgagaag gctgggagc aacgtcgtg gatgttggc 
      gttgacctt tctgaattt tacatgtcc gttgaatgg 
      gacatttta gaagtacct gccgtcaga aacgaaaaa 
      ttctacacg tgctgtgat gagccatat ctcgatata 
      actttcaat ataacgatg cgaagaaag actttgttt 
      tataccgta aacatcatc atcccctgc atgggcatt 
      tcgttcctc accgtgctg accttctac cttccatca 
      gacagcgga gagaaggtt acgctgtca atttcgatt 
      ctaattagt ctccacgtg ttctttctt ctcgtcgtt 
      gaaattatt ccgccgact tcactcgtc gtcccgctt 
      ttaggaaaa tatctcatc tttgccatg attcttgtc 
      tccatcagc atatgtgtc accgtcgtg gtcctgaac 
      gtccatttc cggtcacct cagacccac aaaatgtct 
      ccatgggtc aagcgagtc tttatccac attctgccg 
      cgactacta gtcatgaaa agaccccaa tacgtattt 
      gaaacaaat agtcgtttc ctgatgcga ggccgggaa 
      ccgtcgaca tgtctttat ccttatcac acaacggta 
      cagagagat gactgcagc cccaagagg agatatcca 
      accagaacc ccatccaag gaagattta tctcctagc 
      ttagaagac ggcggccct ttcggcggc agctgtcaa 
      atccacggt cccgtggcc atcccgcac tcggaatcg 
      gaggatctc accgtatca gcggaggcc gataatgga 
      atcaagagc ccagtgctg cgaaatccg ggattttcg 
      cattcgcgt tgtcctccc gaaattcac aaatcgtgc 
      ttctgcgta cgatttatc gcagagcat acgaagatg 
      ttggaggac tcgactaag gtgaaggaa gactggaag 
      tacgtggcg atggtgctg gaccgtttg tttctgtgg 
      atatttacc ttggccgtg ctggtggga acggccggg 
      ataatcctt caagctcct acgttatac gacgatcgc 
      atcccaatc gacaaaaag ttcgaggag tataccacc 
      actacggtt gttcgttgc cctcctcca cagtag
    • >TC000394 RB
      atgtggagt agtctgttg gatccgaat ggcgttatt 
      cgcgaacgg tcgggcggg gtgccgatc tggcatcgt 
      ggttccgaa tcgagcgtg cagtgtggc tccagagtg 
      ccgcggtgg agcgtcggg atgcctcct tctgccgcc 
      gagacgctg agagcctgg ctgctgtcg gccttggta 
      gtgcacggt gctgtggct ggaaatcct gatgcgaag 
      cgtctctac gatgacctg ctttccaac tacaacaag 
      ttagtgcgg cctgtggtc aacacctct gacgtcttg 
      cgagtttgt atcaaactc aaactttca caactcatc 
      gacgtgaac ttaaagaat caaataatg acaacaaat 
      ttgtgggtc gagcaatcg tggtacgac tacaaatta 
      aggtgggag ccgaaagaa tatgggggg gtgcacatg 
      ctccacgtt ccttcggat catatttgg aggccggac 
      atcgtcctt tacaataac gcggacggg aacttcgaa 
      gttaccctg gccaccaag gccaccatc taccaccaa 
      ggtctagtc gaatggaag ccaccagct atatacaag 
      tcctcttgt gagattgac gttgagtat tttccgttc 
      gatgaacag acctgcgtc ctcaaattt ggtagctgg 
      acttatgac ggcttcaag gtggacctg cgacacatg 
      gacgagaag gctgggagc aacgtcgtg gatgttggc 
      gttgacctt tctgaattt tacatgtcc gttgaatgg 
      gacatttta gaagtacct gccgtcaga aacgaaaaa 
      ttctacacg tgctgtgat gagccatat ctcgatata 
      actttcaat ataacgatg cgaagaaag actttgttt 
      tataccgta aacatcatc atcccctgc atgggcatt 
      tcgttcctc accgtgctg accttctac cttccatca 
      gacagcgga gagaaggtt acgctgtca atttcgatt 
      ctaattagt ctccacgtg ttctttctt ctcgtcgtt 
      gaaattatt ccgccgact tcactcgtc gtcccgctt 
      ttaggaaaa tatctcatc tttgccatg attcttgtc 
      tccatcagc atatgtgtc accgtcgtg gtcctgaac 
      gtccatttc cggtcacct cagacccac aaaatgtct 
      ccatgggtc aagcgagtc tttatccac attctgccg 
      cgactacta gtcatgaaa agaccccaa tacgtattt 
      gaaacaaat agtcgtttc ctgatgcga ggccgggaa 
      ccgtcgaca tgtctttat ccttatcac acaacggta 
      cagagagat gactgcagc cccaagagg agatatcca 
      accagaacc ccatccaag gaagattta tctcctagc 
      ttagaagac ggcggccct ttcggcggc agctgtcaa 
      atccacggt cccgtggcc atcccgcac tcggaatcg 
      gaggatctc accgtatca gcggaggcc gataatgga 
      atcaagagc ccagtgctg cgaaatccg ggattttcg 
      cattcgcgt tgtcctccc gaaattcac aaatcgtgc 
      ttctgcgta cgatttatc gcagagcat acgaagatg 
      ttggaggac tcgactaag gtgaaggaa gactggaag 
      tacgtggcg atggtgctg gaccgtttg tttctgtgg 
      atatttacc ttggccgtg ctggtggga acggccggg 
      ataatcctt caagctcct acgttatac gacgatcgc 
      atcccaatc gacaaaaag ttcgaggag tataccacc 
      actacggtt gttcgttgc cctcctcca cagtag
    • >TC000394 RC
      atgtggagt agtctgttg gatccgaat ggcgttatt 
      cgcgaacgg tcgggcggg gtgccgatc tggcatcgt 
      ggttccgaa tcgagcgtg cagtgtggc tccagagtg 
      ccgcggtgg agcgtcggg atgcctcct tctgccgcc 
      gagacgctg agagcctgg ctgctgtcg gccttggta 
      gtgcacggt gctgtggct ggaaatcct gatgcgaag 
      cgtctctac gatgacctg ctttccaac tacaacaag 
      ttagtgcgg cctgtggtc aacacctct gacgtcttg 
      cgagtttgt atcaaactc aaactttca caactcatc 
      gacgtgaac ttaaagaat caaataatg acaacaaat 
      ttgtgggtc gagcaatcg tggtacgac tacaaatta 
      aggtgggag ccgaaagaa tatgggggg gtgcacatg 
      ctccacgtt ccttcggat catatttgg aggccggac 
      atcgtcctt tacaataac gcggacggg aacttcgaa 
      gttaccctg gccaccaag gccaccatc taccaccaa 
      ggtctagtc gaatggaag ccaccagct atatacaag 
      tcctcttgt gagattgac gttgagtat tttccgttc 
      gatgaacag acctgcgtc ctcaaattt ggtagctgg 
      acttatgac ggcttcaag gtgttttgg gtttgtgtc 
      agtggatgt ggcgtgtct ggaaatttt ggtggctgt 
      cccgcgccc agatgtgta aaagcaccc ggaggtaat 
      ggagttgac cgatggcag ccaccccca ggttgcgac 
      gccgacggc aactatgag gtcacattg atgaccaaa 
      gccaccgtc tactacaac ggcctcgtg gtgtggcag 
      ccgcccgcg gtttacaag tcctcgtgc gctatagac 
      gtagaattc tttccgtac gacgtccaa acatgcgtt 
      ttgaagtta ggaagttgg acgtacgat gggtttaag 
      gtggacctg cgacacatg gacgagaag gctgggagc 
      aacgtcgtg gatgttggc gttgacctt tctgaattt 
      tacatgtcc gttgaatgg gacatttta gaagtacct 
      gccgtcaga aacgaaaaa ttctacacg tgctgtgat 
      gagccatat ctcgatata actttcaat ataacgatg 
      cgaagaaag actttgttt tataccgta aacatcatc 
      atcccctgc atgggcatt tcgttcctc accgtgctg 
      accttctac cttccatca gacagcgga gagaaggtt 
      acgctgtca atttcgatt ctaattagt ctccacgtg 
      ttctttctt ctcgtcgtt gaaattatt ccgccgact 
      tcactcgtc gtcccgctt ttaggaaaa tatctcatc 
      tttgccatg attcttgtc tccatcagc atatgtgtc 
      accgtcgtg gtcctgaac gtccatttc cggtcacct 
      cagacccac aaaatgtct ccatgggtc aagcgagtc 
      tttatccac attctgccg cgactacta gtcatgaaa 
      agaccccaa tacgtattt gaaacaaat agtcgtttc 
      ctgatgcga ggccgggaa ccgtcgaca tgtctttat 
      ccttatcac acaacggta cagagagat gactgcagc 
      cccaagagg agatatcca accagaacc ccatccaag 
      gaagattta tctcctagc ttagaagac ggcggccct 
      ttcggcggc agctgtcaa atccacggt cccgtggcc 
      atcccgcac tcggaatcg gaggatctc accgtatca 
      gcggaggcc gataatgga atcaagagc ccagtgctg 
      cgaaatccg ggattttcg cattcgcgt tgtcctccc 
      gaaattcac aaatcgtgc ttctgcgta cgatttatc 
      gcagagcat acgaagatg ttggaggac tcgactaag 
      gtgaaggaa gactggaag tacgtggcg atggtgctg 
      gaccgtttg tttctgtgg atatttacc ttggccgtg 
      ctggtggga acggccggg ataatcctt caagctcct 
      acgttatac gacgatcgc atcccaatc gacaaaaag 
      ttcgaggag tataccacc actacggtt gttcgttgc 
      cctcctcca cagtag
    • >TC000394 RD
      atgtggagt agtctgttg gatccgaat ggcgttatt 
      cgcgaacgg tcgggcggg gtgccgatc tggcatcgt 
      ggttccgaa tcgagcgtg cagtgtggc tccagagtg 
      ccgcggtgg agcgtcggg atgcctcct tctgccgcc 
      gagacgctg agagcctgg ctgctgtcg gccttggta 
      gtgcacggt gctgtggct ggaaatcct gatgcgaag 
      cgtctctac gatgacctg ctttccaac tacaacaag 
      ttagtgcgg cctgtggtc aacacctct gacgtcttg 
      cgagtttgt atcaaactc aaactttca caactcatc 
      gacgtgaac ttaaagaat caaataatg acaacaaat 
      ttgtgggtc gagcaatcg tggtacgac tacaaatta 
      aggtgggag ccgaaagaa tatgggggg gtgcacatg 
      ctccacgtt ccttcggat catatttgg aggccggac 
      atcgtcctt tacaataac gcggacggg aacttcgaa 
      gttaccctg gccaccaag gccaccatc taccaccaa 
      ggtctagtc gaatggaag ccaccagct atatacaag 
      tcctcttgt gagattgac gttgagtat tttccgttc 
      gatgaacag acctgcgtc ctcaaattt ggtagctgg 
      acttatgac ggcttcaag acaccactc actcacatt 
      gacgaattg agaaaagga gcagctggc agtaaacac 
      atgatctca aataacacg gcattgaaa agagcttgg 
      gcagacaat cgggccgca ccgcagatt tatgatcat 
      cacatggca atcaccgcc gacggcaac tatgaggtc 
      acattgatg accaaagcc accgtctac tacaacggc 
      ctcgtggtg tggcagccg cccgcggtt tacaagtcc 
      tcgtgcgct atagacgta gaattcttt ccgtacgac 
      gtccaaaca tgcgttttg aagttagga agttggacg 
      tacgatggg tttaaggtg gacctgcga cacatggac 
      gagaaggct gggagcaac gtcgtggat gttggcgtt 
      gacctttct gaattttac atgtccgtt gaatgggac 
      attttagaa gtacctgcc gtcagaaac gaaaaattc 
      tacacgtgc tgtgatgag ccatatctc gatataact 
      ttcaatata acgatgcga agaaagact ttgttttat 
      accgtaaac atcatcatc ccctgcatg ggcatttcg 
      ttcctcacc gtgctgacc ttctacctt ccatcagac 
      agcggagag aaggttacg ctgtcaatt tcgattcta 
      attagtctc cacgtgttc tttcttctc gtcgttgaa 
      attattccg ccgacttca ctcgtcgtc ccgctttta 
      ggaaaatat ctcatcttt gccatgatt cttgtctcc 
      atcagcata tgtgtcacc gtcgtggtc ctgaacgtc 
      catttccgg tcacctcag acccacaaa atgtctcca 
      tgggtcaag cgagtcttt atccacatt ctgccgcga 
      ctactagtc atgaaaaga ccccaatac gtatttgaa 
      acaaatagt cgtttcctg atgcgaggc cgggaaccg 
      tcgacatgt ctttatcct tatcacaca acggtacag 
      agagatgac tgcagcccc aagaggaga tatccaacc 
      agaacccca tccaaggaa gatttatct cctagctta 
      gaagacggc ggccctttc ggcggcagc tgtcaaatc 
      cacggtccc gtggccatc ccgcactcg gaatcggag 
      gatctcacc gtatcagcg gaggccgat aatggaatc 
      aagagccca gtgctgcga aatccggga ttttcgcat 
      tcgcgttgt cctcccgaa attcacaaa tcgtgcttc 
      tgcgtacga tttatcgca gagcatacg aagatgttg 
      gaggactcg actaaggtg aaggaagac tggaagtac 
      gtggcgatg gtgctggac cgtttgttt ctgtggata 
      tttaccttg gccgtgctg gtgggaacg gccgggata 
      atccttcaa gctcctacg ttatacgac gatcgcatc 
      ccaatcgac aaaaagttc gaggagtat accaccact 
      acggttgtt cgttgccct cctccacag tag
    mRNA sequence
    • >TC000394 RA
      aaagcatta ttttttcgt tgtgtaaac gcagccaca 
      aatgattta ttttcaagt gtgccctac gtggccatg 
      cgactttaa cacgtccag aagcatttt ttgtcttac 
      ctgaaacta aaccaaaag aaccaaaat tttaacatc 
      cttgaattc ggttatatt taattttat gttacgcta 
      ataaaatgt ggagtagtc tgttggatc cgaatggcg 
      ttattcgcg aacggtcgg gcggggtgc cgatctggc 
      atcgtggtt ccgaatcga gcgtgcagt gtggctcca 
      gagtgccgc ggtggagcg tcgggatgc ctccttctg 
      ccgccgaga cgctgagag cctggctgc tgtcggcct 
      tggtagtgc acggtgctg tggctggaa atcctgatg 
      cgaagcgtc tctacgatg acctgcttt ccaactaca 
      acaagttag tgcggcctg tggtcaaca cctctgacg 
      tcttgcgag tttgtatca aactcaaac tttcacaac 
      tcatcgacg tgaacttaa agaatcaaa taatgacaa 
      caaatttgt gggtcgagc aatcgtggt acgactaca 
      aattaaggt gggagccga aagaatatg ggggggtgc 
      acatgctcc acgttcctt cggatcata tttggaggc 
      cggacatcg tcctttaca ataacgccg acggcaact 
      atgaggtca cattgatga ccaaagcca ccgtctact 
      acaacggcc tcgtggtgt ggcagccgc ccgcggttt 
      acaagtcct cgtgcgcta tagacgtag aattctttc 
      cgtacgacg tccaaacat gcgttttga agttaggaa 
      gttggacgt acgatgggt ttaaggtgg acctgcgac 
      acatggacg agaaggctg ggagcaacg tcgtggatg 
      ttggcgttg acctttctg aattttaca tgtccgttg 
      aatgggaca ttttagaag tacctgccg tcagaaacg 
      aaaaattct acacgtgct gtgatgagc catatctcg 
      atataactt tcaatataa cgatgcgaa gaaagactt 
      tgttttata ccgtaaaca tcatcatcc cctgcatgg 
      gcatttcgt tcctcaccg tgctgacct tctaccttc 
      catcagaca gcggagaga aggttacgc tgtcaattt 
      cgattctaa ttagtctcc acgtgttct ttcttctcg 
      tcgttgaaa ttattccgc cgacttcac tcgtcgtcc 
      cgcttttag gaaaatatc tcatctttg ccatgattc 
      ttgtctcca tcagcatat gtgtcaccg tcgtggtcc 
      tgaacgtcc atttccggt cacctcaga cccacaaaa 
      tgtctccat gggtcaagc gagtcttta tccacattc 
      tgccgcgac tactagtca tgaaaagac cccaatacg 
      tatttgaaa caaatagtc gtttcctga tgcgaggcc 
      gggaaccgt cgacatgtc tttatcctt atcacacaa 
      cggtacaga gagatgact gcagcccca agaggagat 
      atccaacca gaaccccat ccaaggaag atttatctc 
      ctagcttag aagacggcg gccctttcg gcggcagct 
      gtcaaatcc acggtcccg tggccatcc cgcactcgg 
      aatcggagg atctcaccg tatcagcgg aggccgata 
      atggaatca agagcccag tgctgcgaa atccgggat 
      tttcgcatt cgcgttgtc ctcccgaaa ttcacaaat 
      cgtgcttct gcgtacgat ttatcgcag agcatacga 
      agatgttgg aggactcga ctaaggtga aggaagact 
      ggaagtacg tggcgatgg tgctggacc gtttgtttc 
      tgtggatat ttaccttgg ccgtgctgg tgggaacgg 
      ccgggataa tccttcaag ctcctacgt tatacgacg 
      atcgcatcc caatcgaca aaaagttcg aggagtata 
      ccaccacta cggttgttc gttgccctc ctccacagt 
      agttgctca cgttattgc ctatatcat tccataaat 
      atcgaatat catcgttgt tgtgtaaat aaaactcat 
      caaaataat tttataatg cagttggag cacaacgac 
      gttctgttt acttgagcg catagtttt tgggtaaac 
      agaaattac gcaatgtgt gactgttaa aaattcatc 
      tcgtcctac aaattcgtc gactgcaaa acgacagga 
      aaaaagctt atgaatttt gtattcaat tgaaagcta 
      cctgaattg taaataagc atcaataat tatatattt 
      tacacagct cggtacgtt ttctattta taaaaagat 
      tttacggag actataggt tggtttttt gtatgtttt 
      taaacacgt agtgccatt atcgaattt aattacggt 
      tcggaagat tttaaataa aatgatgtt taaatagca 
      cacaattaa acaaaatga aatgtatct aaataatag 
      acaaacgta aaactagac atttaaatg aataaataa 
      atattagac tagatagaa gaaattgag taaatattt 
      agatcaata gactgccta ttgacataa tgggaacat 
      aatttgtac atataatgt atgttagat tggtcagcc 
      ctatcgtta aattgtgtt gtaccatag tttaaaatt 
      gttatacgt tgttgcttg gttttataa atatccatg 
      cttaataaa gtatttcga acaca
    • >TC000394 RB
      aaagcatta ttttttcgt tgtgtaaac gcagccaca 
      aatgattta ttttcaagt gtgccctac gtggccatg 
      cgactttaa cacgtccag aagcatttt ttgtcttac 
      ctgaaacta aaccaaaag aaccaaaat tttaacatc 
      cttgaattc ggttatatt taattttat gttacgcta 
      ataaaatgt ggagtagtc tgttggatc cgaatggcg 
      ttattcgcg aacggtcgg gcggggtgc cgatctggc 
      atcgtggtt ccgaatcga gcgtgcagt gtggctcca 
      gagtgccgc ggtggagcg tcgggatgc ctccttctg 
      ccgccgaga cgctgagag cctggctgc tgtcggcct 
      tggtagtgc acggtgctg tggctggaa atcctgatg 
      cgaagcgtc tctacgatg acctgcttt ccaactaca 
      acaagttag tgcggcctg tggtcaaca cctctgacg 
      tcttgcgag tttgtatca aactcaaac tttcacaac 
      tcatcgacg tgaacttaa agaatcaaa taatgacaa 
      caaatttgt gggtcgagc aatcgtggt acgactaca 
      aattaaggt gggagccga aagaatatg ggggggtgc 
      acatgctcc acgttcctt cggatcata tttggaggc 
      cggacatcg tcctttaca ataacgcgg acgggaact 
      tcgaagtta ccctggcca ccaaggcca ccatctacc 
      accaaggtc tagtcgaat ggaagccac cagctatat 
      acaagtcct cttgtgaga ttgacgttg agtattttc 
      cgttcgatg aacagacct gcgtcctca aatttggta 
      gctggactt atgacggct tcaaggtgg acctgcgac 
      acatggacg agaaggctg ggagcaacg tcgtggatg 
      ttggcgttg acctttctg aattttaca tgtccgttg 
      aatgggaca ttttagaag tacctgccg tcagaaacg 
      aaaaattct acacgtgct gtgatgagc catatctcg 
      atataactt tcaatataa cgatgcgaa gaaagactt 
      tgttttata ccgtaaaca tcatcatcc cctgcatgg 
      gcatttcgt tcctcaccg tgctgacct tctaccttc 
      catcagaca gcggagaga aggttacgc tgtcaattt 
      cgattctaa ttagtctcc acgtgttct ttcttctcg 
      tcgttgaaa ttattccgc cgacttcac tcgtcgtcc 
      cgcttttag gaaaatatc tcatctttg ccatgattc 
      ttgtctcca tcagcatat gtgtcaccg tcgtggtcc 
      tgaacgtcc atttccggt cacctcaga cccacaaaa 
      tgtctccat gggtcaagc gagtcttta tccacattc 
      tgccgcgac tactagtca tgaaaagac cccaatacg 
      tatttgaaa caaatagtc gtttcctga tgcgaggcc 
      gggaaccgt cgacatgtc tttatcctt atcacacaa 
      cggtacaga gagatgact gcagcccca agaggagat 
      atccaacca gaaccccat ccaaggaag atttatctc 
      ctagcttag aagacggcg gccctttcg gcggcagct 
      gtcaaatcc acggtcccg tggccatcc cgcactcgg 
      aatcggagg atctcaccg tatcagcgg aggccgata 
      atggaatca agagcccag tgctgcgaa atccgggat 
      tttcgcatt cgcgttgtc ctcccgaaa ttcacaaat 
      cgtgcttct gcgtacgat ttatcgcag agcatacga 
      agatgttgg aggactcga ctaaggtga aggaagact 
      ggaagtacg tggcgatgg tgctggacc gtttgtttc 
      tgtggatat ttaccttgg ccgtgctgg tgggaacgg 
      ccgggataa tccttcaag ctcctacgt tatacgacg 
      atcgcatcc caatcgaca aaaagttcg aggagtata 
      ccaccacta cggttgttc gttgccctc ctccacagt 
      agttgctca cgttattgc ctatatcat tccataaat 
      atcgaatat catcgttgt tgtgtaaat aaaactcat 
      caaaataat tttataatg cagttggag cacaacgac 
      gttctgttt acttgagcg catagtttt tgggtaaac 
      agaaattac gcaatgtgt gactgttaa aaattcatc 
      tcgtcctac aaattcgtc gactgcaaa acgacagga 
      aaaaagctt atgaatttt gtattcaat tgaaagcta 
      cctgaattg taaataagc atcaataat tatatattt 
      tacacagct cggtacgtt ttctattta taaaaagat 
      tttacggag actataggt tggtttttt gtatgtttt 
      taaacacgt agtgccatt atcgaattt aattacggt 
      tcggaagat tttaaataa aatgatgtt taaatagca 
      cacaattaa acaaaatga aatgtatct aaataatag 
      acaaacgta aaactagac atttaaatg aataaataa 
      atattagac tagatagaa gaaattgag taaatattt 
      agatcaata gactgccta ttgacataa tgggaacat 
      aatttgtac atataatgt atgttagat tggtcagcc 
      ctatcgtta aattgtgtt gtaccatag tttaaaatt 
      gttatacgt tgttgcttg gttttataa atatccatg 
      cttaataaa gtatttcga acaca
    • >TC000394 RC
      aaagcatta ttttttcgt tgtgtaaac gcagccaca 
      aatgattta ttttcaagt gtgccctac gtggccatg 
      cgactttaa cacgtccag aagcatttt ttgtcttac 
      ctgaaacta aaccaaaag aaccaaaat tttaacatc 
      cttgaattc ggttatatt taattttat gttacgcta 
      ataaaatgt ggagtagtc tgttggatc cgaatggcg 
      ttattcgcg aacggtcgg gcggggtgc cgatctggc 
      atcgtggtt ccgaatcga gcgtgcagt gtggctcca 
      gagtgccgc ggtggagcg tcgggatgc ctccttctg 
      ccgccgaga cgctgagag cctggctgc tgtcggcct 
      tggtagtgc acggtgctg tggctggaa atcctgatg 
      cgaagcgtc tctacgatg acctgcttt ccaactaca 
      acaagttag tgcggcctg tggtcaaca cctctgacg 
      tcttgcgag tttgtatca aactcaaac tttcacaac 
      tcatcgacg tgaacttaa agaatcaaa taatgacaa 
      caaatttgt gggtcgagc aatcgtggt acgactaca 
      aattaaggt gggagccga aagaatatg ggggggtgc 
      acatgctcc acgttcctt cggatcata tttggaggc 
      cggacatcg tcctttaca ataacgcgg acgggaact 
      tcgaagtta ccctggcca ccaaggcca ccatctacc 
      accaaggtc tagtcgaat ggaagccac cagctatat 
      acaagtcct cttgtgaga ttgacgttg agtattttc 
      cgttcgatg aacagacct gcgtcctca aatttggta 
      gctggactt atgacggct tcaaggtgt tttgggttt 
      gtgtcagtg gatgtggcg tgtctggaa attttggtg 
      gctgtcccg cgcccagat gtgtaaaag cacccggag 
      gtaatggag ttgaccgat ggcagccac ccccaggtt 
      gcgacgccg acggcaact atgaggtca cattgatga 
      ccaaagcca ccgtctact acaacggcc tcgtggtgt 
      ggcagccgc ccgcggttt acaagtcct cgtgcgcta 
      tagacgtag aattctttc cgtacgacg tccaaacat 
      gcgttttga agttaggaa gttggacgt acgatgggt 
      ttaaggtgg acctgcgac acatggacg agaaggctg 
      ggagcaacg tcgtggatg ttggcgttg acctttctg 
      aattttaca tgtccgttg aatgggaca ttttagaag 
      tacctgccg tcagaaacg aaaaattct acacgtgct 
      gtgatgagc catatctcg atataactt tcaatataa 
      cgatgcgaa gaaagactt tgttttata ccgtaaaca 
      tcatcatcc cctgcatgg gcatttcgt tcctcaccg 
      tgctgacct tctaccttc catcagaca gcggagaga 
      aggttacgc tgtcaattt cgattctaa ttagtctcc 
      acgtgttct ttcttctcg tcgttgaaa ttattccgc 
      cgacttcac tcgtcgtcc cgcttttag gaaaatatc 
      tcatctttg ccatgattc ttgtctcca tcagcatat 
      gtgtcaccg tcgtggtcc tgaacgtcc atttccggt 
      cacctcaga cccacaaaa tgtctccat gggtcaagc 
      gagtcttta tccacattc tgccgcgac tactagtca 
      tgaaaagac cccaatacg tatttgaaa caaatagtc 
      gtttcctga tgcgaggcc gggaaccgt cgacatgtc 
      tttatcctt atcacacaa cggtacaga gagatgact 
      gcagcccca agaggagat atccaacca gaaccccat 
      ccaaggaag atttatctc ctagcttag aagacggcg 
      gccctttcg gcggcagct gtcaaatcc acggtcccg 
      tggccatcc cgcactcgg aatcggagg atctcaccg 
      tatcagcgg aggccgata atggaatca agagcccag 
      tgctgcgaa atccgggat tttcgcatt cgcgttgtc 
      ctcccgaaa ttcacaaat cgtgcttct gcgtacgat 
      ttatcgcag agcatacga agatgttgg aggactcga 
      ctaaggtga aggaagact ggaagtacg tggcgatgg 
      tgctggacc gtttgtttc tgtggatat ttaccttgg 
      ccgtgctgg tgggaacgg ccgggataa tccttcaag 
      ctcctacgt tatacgacg atcgcatcc caatcgaca 
      aaaagttcg aggagtata ccaccacta cggttgttc 
      gttgccctc ctccacagt agttgctca cgttattgc 
      ctatatcat tccataaat atcgaatat catcgttgt 
      tgtgtaaat aaaactcat caaaataat tttataatg 
      cagttggag cacaacgac gttctgttt acttgagcg 
      catagtttt tgggtaaac agaaattac gcaatgtgt 
      gactgttaa aaattcatc tcgtcctac aaattcgtc 
      gactgcaaa acgacagga aaaaagctt atgaatttt 
      gtattcaat tgaaagcta cctgaattg taaataagc 
      atcaataat tatatattt tacacagct cggtacgtt 
      ttctattta taaaaagat tttacggag actataggt 
      tggtttttt gtatgtttt taaacacgt agtgccatt 
      atcgaattt aattacggt tcggaagat tttaaataa 
      aatgatgtt taaatagca cacaattaa acaaaatga 
      aatgtatct aaataatag acaaacgta aaactagac 
      atttaaatg aataaataa atattagac tagatagaa 
      gaaattgag taaatattt agatcaata gactgccta 
      ttgacataa tgggaacat aatttgtac atataatgt 
      atgttagat tggtcagcc ctatcgtta aattgtgtt 
      gtaccatag tttaaaatt gttatacgt tgttgcttg 
      gttttataa atatccatg cttaataaa gtatttcga 
      acaca
    • >TC000394 RD
      aaagcatta ttttttcgt tgtgtaaac gcagccaca 
      aatgattta ttttcaagt gtgccctac gtggccatg 
      cgactttaa cacgtccag aagcatttt ttgtcttac 
      ctgaaacta aaccaaaag aaccaaaat tttaacatc 
      cttgaattc ggttatatt taattttat gttacgcta 
      ataaaatgt ggagtagtc tgttggatc cgaatggcg 
      ttattcgcg aacggtcgg gcggggtgc cgatctggc 
      atcgtggtt ccgaatcga gcgtgcagt gtggctcca 
      gagtgccgc ggtggagcg tcgggatgc ctccttctg 
      ccgccgaga cgctgagag cctggctgc tgtcggcct 
      tggtagtgc acggtgctg tggctggaa atcctgatg 
      cgaagcgtc tctacgatg acctgcttt ccaactaca 
      acaagttag tgcggcctg tggtcaaca cctctgacg 
      tcttgcgag tttgtatca aactcaaac tttcacaac 
      tcatcgacg tgaacttaa agaatcaaa taatgacaa 
      caaatttgt gggtcgagc aatcgtggt acgactaca 
      aattaaggt gggagccga aagaatatg ggggggtgc 
      acatgctcc acgttcctt cggatcata tttggaggc 
      cggacatcg tcctttaca ataacgcgg acgggaact 
      tcgaagtta ccctggcca ccaaggcca ccatctacc 
      accaaggtc tagtcgaat ggaagccac cagctatat 
      acaagtcct cttgtgaga ttgacgttg agtattttc 
      cgttcgatg aacagacct gcgtcctca aatttggta 
      gctggactt atgacggct tcaagacac cactcactc 
      acattgacg aattgagaa aaggagcag ctggcagta 
      aacacatga tctcaaata acacggcat tgaaaagag 
      cttgggcag acaatcggg ccgcaccgc agatttatg 
      atcatcaca tggcaatca ccgccgacg gcaactatg 
      aggtcacat tgatgacca aagccaccg tctactaca 
      acggcctcg tggtgtggc agccgcccg cggtttaca 
      agtcctcgt gcgctatag acgtagaat tctttccgt 
      acgacgtcc aaacatgcg ttttgaagt taggaagtt 
      ggacgtacg atgggttta aggtggacc tgcgacaca 
      tggacgaga aggctggga gcaacgtcg tggatgttg 
      gcgttgacc tttctgaat tttacatgt ccgttgaat 
      gggacattt tagaagtac ctgccgtca gaaacgaaa 
      aattctaca cgtgctgtg atgagccat atctcgata 
      taactttca atataacga tgcgaagaa agactttgt 
      tttataccg taaacatca tcatcccct gcatgggca 
      tttcgttcc tcaccgtgc tgaccttct accttccat 
      cagacagcg gagagaagg ttacgctgt caatttcga 
      ttctaatta gtctccacg tgttctttc ttctcgtcg 
      ttgaaatta ttccgccga cttcactcg tcgtcccgc 
      ttttaggaa aatatctca tctttgcca tgattcttg 
      tctccatca gcatatgtg tcaccgtcg tggtcctga 
      acgtccatt tccggtcac ctcagaccc acaaaatgt 
      ctccatggg tcaagcgag tctttatcc acattctgc 
      cgcgactac tagtcatga aaagacccc aatacgtat 
      ttgaaacaa atagtcgtt tcctgatgc gaggccggg 
      aaccgtcga catgtcttt atccttatc acacaacgg 
      tacagagag atgactgca gccccaaga ggagatatc 
      caaccagaa ccccatcca aggaagatt tatctccta 
      gcttagaag acggcggcc ctttcggcg gcagctgtc 
      aaatccacg gtcccgtgg ccatcccgc actcggaat 
      cggaggatc tcaccgtat cagcggagg ccgataatg 
      gaatcaaga gcccagtgc tgcgaaatc cgggatttt 
      cgcattcgc gttgtcctc ccgaaattc acaaatcgt 
      gcttctgcg tacgattta tcgcagagc atacgaaga 
      tgttggagg actcgacta aggtgaagg aagactgga 
      agtacgtgg cgatggtgc tggaccgtt tgtttctgt 
      ggatattta ccttggccg tgctggtgg gaacggccg 
      ggataatcc ttcaagctc ctacgttat acgacgatc 
      gcatcccaa tcgacaaaa agttcgagg agtatacca 
      ccactacgg ttgttcgtt gccctcctc cacagtagt 
      tgctcacgt tattgccta tatcattcc ataaatatc 
      gaatatcat cgttgttgt gtaaataaa actcatcaa 
      aataatttt ataatgcag ttggagcac aacgacgtt 
      ctgtttact tgagcgcat agtttttgg gtaaacaga 
      aattacgca atgtgtgac tgttaaaaa ttcatctcg 
      tcctacaaa ttcgtcgac tgcaaaacg acaggaaaa 
      aagcttatg aattttgta ttcaattga aagctacct 
      gaattgtaa ataagcatc aataattat atattttac 
      acagctcgg tacgttttc tatttataa aaagatttt 
      acggagact ataggttgg ttttttgta tgtttttaa 
      acacgtagt gccattatc gaatttaat tacggttcg 
      gaagatttt aaataaaat gatgtttaa atagcacac 
      aattaaaca aaatgaaat gtatctaaa taatagaca 
      aacgtaaaa ctagacatt taaatgaat aaataaata 
      ttagactag atagaagaa attgagtaa atatttaga 
      tcaatagac tgcctattg acataatgg gaacataat 
      ttgtacata taatgtatg ttagattgg tcagcccta 
      tcgttaaat tgtgttgta ccatagttt aaaattgtt 
      atacgttgt tgcttggtt ttataaata tccatgctt 
      aataaagta tttcgaaca ca
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