Status
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Gene information
General information
TC010883 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4971803..4973974
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010883
    iBeetle screen iB_07300

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010883 RA
      MPISRLDVF YRKILLSKC FTKGWGDPE NLMELFNFR 
      KLVSNRNAC YELLPKDYP VDIINVQRF TDFQILEGR 
      LWTPFRMFL PSLLVPEIQ QVYFQMILP RKWPSSDYR 
      PLCIHLAGT GDHYFWRRR NFMAKPLLQ AGIGSLIVE 
      NPYYGTRKP KDQLRSSLH YVSDIFVMG GCLILETLA 
      LLNWCEQIG FGPLGVSGI SMGGHMASL AASNWPKPL 
      VLVPCLSWS TASSVFTEG VMSESIDWD MLQKQLFSN 
      KIYCDTLSK SCKIVDSPF ACTLSRIPE YGLTSLLEQ 
      KSDAGYISP YEMMNLLDS SNCPAISYK HSSQNVKVE 
      SSEYSVMNL PIFNFSREI TDMRKRDKE AIWFMRGMM 
      DEFTHLKNF SVPYEPSLI IAVCAESDG YVPRVGISK 
      LDEIWPGAT IKYVDTGHV GAYLWHRKL FRFVSRKHL 
      EPFFRRVFV CLGSLINK
    • >TC010883 RB
      MPISRLDVF YRKILLSKC FTKGWGDPE NLMELFNFR 
      KLVSNRNAC YELLPKDYP VDIINVQRF TDFQILEGR 
      LWTPFRMFL PSLLVPEIQ QVYFQMILP RKWPSSDYR 
      PLCIHLAGT GDHYFWRRR NFMAKPLLQ AGIGSLIVE 
      NPYYGTRKP KDQLRSSLH YVSDIFVMG GCLILETLA 
      LLNWCEQIG FGPLGVSGI SMGGHMASL AASNWPKPL 
      VLVPCLSWS TASSVFTEG VMSESIDWD MLQKQLFSN 
      KIYCDTLSK SCKIVDSPF ACTLSRIPE YGLTSLLEQ 
      KSDAGYISP YEMMNLLDS SNCPAISYK HSSQNVKVE 
      SSEYSVMNL PIFNFSREI TDMRKRDKE AIWFMRGMM 
      DEFTHLKNF SVPYEPSLI IAVCAESDG YVPRVGISK 
      LDEIWPGAT IKYVDTGHV GAYLWHRKL FRDCIIQAF 
      ERAKKIAPK PGPYFEN
    CDS sequence
    • >TC010883 RA
      atgccgatt agccggctt gacgtgttt taccgcaaa 
      atactgtta tcaaagtgc ttcaccaag ggatggggc 
      gatcctgag aatttgatg gaattgttt aattttcgt 
      aaactcgtc tcaaatcgc aacgcctgt tatgaatta 
      ttaccaaaa gactatccg gtagacata atcaacgtg 
      cagcggttc accgacttc cagatactc gagggtaga 
      ttatggacc ccctttcgc atgtttcta ccgtcgctg 
      cttgtgcca gaaattcag caagtttat tttcaaatg 
      atactgccc cgaaaatgg ccctcgagt gactacaga 
      cctttgtgt attcacctc gcggggacc ggcgatcat 
      tatttctgg cgccgccgg aatttcatg gcaaagccg 
      ctgcttcaa gccgggatt gggtcccta atcgtggag 
      aacccctac tacggcacc aggaagccc aaggaccag 
      ctgcgctcg agcctccac tacgtctcc gatattttc 
      gtgatgggc gggtgcctg attctggag acgctggcg 
      cttttgaac tggtgcgag cagatcggg ttcggcccc 
      ctgggggtg tcgggcatt tcgatgggc ggacatatg 
      gcttccttg gcggcctct aactggcca aagccctta 
      gtgcttgtt ccttgcttg tcatggtcg actgcctcc 
      tcggttttt acagaggga gttatgagt gaatctatt 
      gattgggac atgctgcaa aagcagttg ttttcaaac 
      aaaatttac tgtgatact ttgtcaaag tcgtgtaaa 
      attgttgat agtccgttt gcatgcact ttaagtaga 
      atcccagag tatgggttg acgtccttg ttggagcaa 
      aaaagtgac gctggatac atttctccg tacgaaatg 
      atgaatctg ctggattcg agcaactgt cctgccatt 
      tcttataag cattctagt cagaacgtt aaagtagag 
      tctagtgaa tactcagtg atgaatttg ccgattttt 
      aactttagt cgcgagata acggatatg agaaagcgc 
      gataaagag gcgatttgg tttatgagg gggatgatg 
      gatgagttc acacacttg aaaaatttc tcggtgcct 
      tatgagccg tcgcttatc atagccgtc tgcgctgag 
      tctgacggg tatgttcct cgggtcggt atttcgaag 
      ctggatgag atttggccg ggagccacc ataaagtat 
      gtcgatacc ggacatgta ggggcctac ctttggcat 
      agaaaatta tttaggttt gtatcacgt aaacacctt 
      gaaccattt tttcggcgc gtgtttgtc tgtcttggc 
      tccttgatc aataaataa
    • >TC010883 RB
      atgccgatt agccggctt gacgtgttt taccgcaaa 
      atactgtta tcaaagtgc ttcaccaag ggatggggc 
      gatcctgag aatttgatg gaattgttt aattttcgt 
      aaactcgtc tcaaatcgc aacgcctgt tatgaatta 
      ttaccaaaa gactatccg gtagacata atcaacgtg 
      cagcggttc accgacttc cagatactc gagggtaga 
      ttatggacc ccctttcgc atgtttcta ccgtcgctg 
      cttgtgcca gaaattcag caagtttat tttcaaatg 
      atactgccc cgaaaatgg ccctcgagt gactacaga 
      cctttgtgt attcacctc gcggggacc ggcgatcat 
      tatttctgg cgccgccgg aatttcatg gcaaagccg 
      ctgcttcaa gccgggatt gggtcccta atcgtggag 
      aacccctac tacggcacc aggaagccc aaggaccag 
      ctgcgctcg agcctccac tacgtctcc gatattttc 
      gtgatgggc gggtgcctg attctggag acgctggcg 
      cttttgaac tggtgcgag cagatcggg ttcggcccc 
      ctgggggtg tcgggcatt tcgatgggc ggacatatg 
      gcttccttg gcggcctct aactggcca aagccctta 
      gtgcttgtt ccttgcttg tcatggtcg actgcctcc 
      tcggttttt acagaggga gttatgagt gaatctatt 
      gattgggac atgctgcaa aagcagttg ttttcaaac 
      aaaatttac tgtgatact ttgtcaaag tcgtgtaaa 
      attgttgat agtccgttt gcatgcact ttaagtaga 
      atcccagag tatgggttg acgtccttg ttggagcaa 
      aaaagtgac gctggatac atttctccg tacgaaatg 
      atgaatctg ctggattcg agcaactgt cctgccatt 
      tcttataag cattctagt cagaacgtt aaagtagag 
      tctagtgaa tactcagtg atgaatttg ccgattttt 
      aactttagt cgcgagata acggatatg agaaagcgc 
      gataaagag gcgatttgg tttatgagg gggatgatg 
      gatgagttc acacacttg aaaaatttc tcggtgcct 
      tatgagccg tcgcttatc atagccgtc tgcgctgag 
      tctgacggg tatgttcct cgggtcggt atttcgaag 
      ctggatgag atttggccg ggagccacc ataaagtat 
      gtcgatacc ggacatgta ggggcctac ctttggcat 
      agaaaatta tttagggac tgcattatt caagccttc 
      gaaagggcg aaaaaaatt gctccgaag ccagggccc 
      tattttgaa aactga
    mRNA sequence
    • >TC010883 RA
      tagtttttg tgcaataaa attaacaca agaattcgt 
      gcaatcgtt atttattat ttgccaggt atttaaatt 
      tagtgacaa cgtgataat tttattttt ggtgcgtta 
      ccaaacacg tcatgatct ttcagtagt ttttcattt 
      ttgaactaa agggggcca aaaagtagt ttttaacag 
      tttaaaaaa atgccgatt agccggctt gacgtgttt 
      taccgcaaa atactgtta tcaaagtgc ttcaccaag 
      ggatggggc gatcctgag aatttgatg gaattgttt 
      aattttcgt aaactcgtc tcaaatcgc aacgcctgt 
      tatgaatta ttaccaaaa gactatccg gtagacata 
      atcaacgtg cagcggttc accgacttc cagatactc 
      gagggtaga ttatggacc ccctttcgc atgtttcta 
      ccgtcgctg cttgtgcca gaaattcag caagtttat 
      tttcaaatg atactgccc cgaaaatgg ccctcgagt 
      gactacaga cctttgtgt attcacctc gcggggacc 
      ggcgatcat tatttctgg cgccgccgg aatttcatg 
      gcaaagccg ctgcttcaa gccgggatt gggtcccta 
      atcgtggag aacccctac tacggcacc aggaagccc 
      aaggaccag ctgcgctcg agcctccac tacgtctcc 
      gatattttc gtgatgggc gggtgcctg attctggag 
      acgctggcg cttttgaac tggtgcgag cagatcggg 
      ttcggcccc ctgggggtg tcgggcatt tcgatgggc 
      ggacatatg gcttccttg gcggcctct aactggcca 
      aagccctta gtgcttgtt ccttgcttg tcatggtcg 
      actgcctcc tcggttttt acagaggga gttatgagt 
      gaatctatt gattgggac atgctgcaa aagcagttg 
      ttttcaaac aaaatttac tgtgatact ttgtcaaag 
      tcgtgtaaa attgttgat agtccgttt gcatgcact 
      ttaagtaga atcccagag tatgggttg acgtccttg 
      ttggagcaa aaaagtgac gctggatac atttctccg 
      tacgaaatg atgaatctg ctggattcg agcaactgt 
      cctgccatt tcttataag cattctagt cagaacgtt 
      aaagtagag tctagtgaa tactcagtg atgaatttg 
      ccgattttt aactttagt cgcgagata acggatatg 
      agaaagcgc gataaagag gcgatttgg tttatgagg 
      gggatgatg gatgagttc acacacttg aaaaatttc 
      tcggtgcct tatgagccg tcgcttatc atagccgtc 
      tgcgctgag tctgacggg tatgttcct cgggtcggt 
      atttcgaag ctggatgag atttggccg ggagccacc 
      ataaagtat gtcgatacc ggacatgta ggggcctac 
      ctttggcat agaaaatta tttaggttt gtatcacgt 
      aaacacctt gaaccattt tttcggcgc gtgtttgtc 
      tgtcttggc tccttgatc aataaataa agcgagtct 
      agtctagcc cttgtatgg aggtcactg actcagacg 
      gattttcaa acaagaaat caagttcaa ggtccgcag 
      acgaaaacg catgctcac gcacgagcc gatgtttat 
      tggtttttt cagggactg cattattca agccttcga 
      aagggcgaa aaaaattgc tccgaagcc agggcccta 
      ttttgaaaa ctgagttag ttcgtgccg ttcctgttg 
      ttttgtttg tattgattt tttaaaagt ttaactttg 
      tacctgact gaggtcttg tgataattt tgctaaaat 
      aaagatttg ttcaatga
    • >TC010883 RB
      tagtttttg tgcaataaa attaacaca agaattcgt 
      gcaatcgtt atttattat ttgccaggt atttaaatt 
      tagtgacaa cgtgataat tttattttt ggtgcgtta 
      ccaaacacg tcatgatct ttcagtagt ttttcattt 
      ttgaactaa agggggcca aaaagtagt ttttaacag 
      tttaaaaaa atgccgatt agccggctt gacgtgttt 
      taccgcaaa atactgtta tcaaagtgc ttcaccaag 
      ggatggggc gatcctgag aatttgatg gaattgttt 
      aattttcgt aaactcgtc tcaaatcgc aacgcctgt 
      tatgaatta ttaccaaaa gactatccg gtagacata 
      atcaacgtg cagcggttc accgacttc cagatactc 
      gagggtaga ttatggacc ccctttcgc atgtttcta 
      ccgtcgctg cttgtgcca gaaattcag caagtttat 
      tttcaaatg atactgccc cgaaaatgg ccctcgagt 
      gactacaga cctttgtgt attcacctc gcggggacc 
      ggcgatcat tatttctgg cgccgccgg aatttcatg 
      gcaaagccg ctgcttcaa gccgggatt gggtcccta 
      atcgtggag aacccctac tacggcacc aggaagccc 
      aaggaccag ctgcgctcg agcctccac tacgtctcc 
      gatattttc gtgatgggc gggtgcctg attctggag 
      acgctggcg cttttgaac tggtgcgag cagatcggg 
      ttcggcccc ctgggggtg tcgggcatt tcgatgggc 
      ggacatatg gcttccttg gcggcctct aactggcca 
      aagccctta gtgcttgtt ccttgcttg tcatggtcg 
      actgcctcc tcggttttt acagaggga gttatgagt 
      gaatctatt gattgggac atgctgcaa aagcagttg 
      ttttcaaac aaaatttac tgtgatact ttgtcaaag 
      tcgtgtaaa attgttgat agtccgttt gcatgcact 
      ttaagtaga atcccagag tatgggttg acgtccttg 
      ttggagcaa aaaagtgac gctggatac atttctccg 
      tacgaaatg atgaatctg ctggattcg agcaactgt 
      cctgccatt tcttataag cattctagt cagaacgtt 
      aaagtagag tctagtgaa tactcagtg atgaatttg 
      ccgattttt aactttagt cgcgagata acggatatg 
      agaaagcgc gataaagag gcgatttgg tttatgagg 
      gggatgatg gatgagttc acacacttg aaaaatttc 
      tcggtgcct tatgagccg tcgcttatc atagccgtc 
      tgcgctgag tctgacggg tatgttcct cgggtcggt 
      atttcgaag ctggatgag atttggccg ggagccacc 
      ataaagtat gtcgatacc ggacatgta ggggcctac 
      ctttggcat agaaaatta tttagggac tgcattatt 
      caagccttc gaaagggcg aaaaaaatt gctccgaag 
      ccagggccc tattttgaa aactgagtt agttcgtgc 
      cgttcctgt tgttttgtt tgtattgat tttttaaaa 
      gtttaactt tgtacctga ctgaggtct tgtgataat 
      tttgctaaa ataaagatt tgttcaatg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY