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Gene information
General information
TC012646 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12467737..12475521
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012646
      iBeetle screen iB_07298

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      remarks: eggs lost, low validity due to technical reasons - should be repeated: egg number not scorable,
      Adult morphology
      remarks: eggs lost, low validity due to technical reasons - should be repeated: egg number not scorable,
      Analysis of embryonic musculature and early development
      remarks: eggs lost, (egglay)
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012646 RA
        MKITNKYPR NVAENYRKY KVGGDFTDQ KNTALATVK 
        VFEALEPRL SDISDVEEE LMRNHALAL VQKEQNLPL 
        IEHLRLKRF DFFQKLSDN AVKTYELIQ RSDLYPNKI 
        EQYLDFTYN PGLHTCDPC IGEKSNEIK TTIKKKPRK 
        CERSEPDVT PPFQAAPHQ IIFWNYELG QVYRQKFKI 
        INTTRSPQN FNYYLFPKD SPFRLKLLT DSQRVAPGM 
        SAEFLVEFR PQTFQNCTE FVKFRTIKG ADLSVLVSS 
        ARDSPKLAC YIFNPFLPW YAVRKPGPG SKYFSERRR 
        TALNFTIDC GTCLLGNHS LVTLIMKND GVRGRFLVT 
        TENEWVFSE LNVRACEIS HGCFTIFPA FFEIDSGEI 
        GQIAVAFVP DKVGVFTEK FVIICDNNS HLEIEILGD 
        CILYSDKFL KVNMYLLEH TFSYSNRLL SINLAPRQT 
        SSVIFSILN NSPVYFNYY WLLKTSTDE KYAGKKMDP 
        EWIRILYGT RFLPTFSTT DVIVQLTAD TEEEGFYYI 
        FLSFYLKNI PLVSLEAYD TKTHVINNY KCDSSCTIE 
        QDNGENLFT DSQLGDVHV FELEINCTV KQPEPVKLA 
        TVNVSLDIP TQGVSPPVS LRLSKRVLN FGIVPKGVS 
        VSQSISVEN LTDDEIEWT IMEIHCTDK SYLCVSSTA 
        GDLDHYSGT LKGKQKKKL HYTIEAKDA KRWVSILIL 
        FACPSCTCQ KYTLEYICI VAYQVAEIN ISLRTDTQL 
        SVLCPREFI YVEIPTIHT IYVHNSGLV VGCFYFLNP 
        VGADADKLR LRFRPQSGV VQPGAAVEV RVELTALKE 
        GLLDRVHVP CFVGDPERL IALRVLCSV ATINVHFYL 
        PDGDRGYRR YIWPPKSEM PAWDHSMGS RGEGGFRGD 
        KGESVSDGE GGDLGDSLL SLIGGDSVA GTYPTIKRR 
        HVGVREATR VSFYFENLT PIKGRFAVE AANYGPASE 
        CFSHVLVKA RTRTREFFW ERACLDGYG IVIEPVYRS 
        GDIIPYEVV EIDLWIYAN TWGKYLEEI IVEIGDLTP 
        FCFNLMVEV VGPPIEYPF ARNTTLPCP TLRFENIAY 
        ATESTTTCF TVTNISAVP IQISWHTYY DNEHKPVSA 
        PVNFLLEFN DDEVQFVMS RDYYGCEAC SKCIMIEPW 
        DLVLEAQET ATVSVTISA SGFNPSCDK TKLKYTLLG 
        FIGLRNSDK FKENYFKRL SGVDLTPTK VEVLVTMEL 
        PIMQVDIAS DETTIDIFV NDLLLKNHK IPTVYLLLR 
        NIDMSSIIF SIEIEKPFK IVEARTIPG GITDQKNLI 
        LERHECLQV AVTCRLTSQ DLFDYSEFI YKSEDNSTV 
        ENYTSVISK DLNVFQKGA KRQIRRVSP ISHQEKHDF 
        DLQHDRSFN KFRNSQNNR T
      CDS sequence
      • >TC012646 RA
        atgaagatc acgaataaa tacccccga aatgttgcc 
        gaaaactac cgaaaatac aaagtcggg ggcgatttt 
        accgaccag aaaaacacg gctttggcc acagtcaaa 
        gtcttcgag gctcttgag ccgcgcttg agcgacatt 
        tccgacgtg gaagaggag ctgatgcgc aaccacgcg 
        ctcgctttg gtgcaaaaa gaacaaaat ttgccactc 
        atcgaacac ttgcgcctc aagagattc gacttcttc 
        caaaaacta tcagacaat gctgtcaag acttacgag 
        ctaattcag cgaagcgat ctgtacccg aacaaaatt 
        gaacagtac ttagatttc acctacaat cctggcttg 
        cacacgtgc gacccttgc attggtgaa aaatcaaac 
        gaaataaaa accaccata aaaaagaaa ccacggaaa 
        tgtgagcga agcgaaccc gacgtgacc ccacctttc 
        caggcagcg ccccaccaa ataattttc tggaactac 
        gagctcggt caagtctac aggcaaaaa ttcaaaatt 
        attaacacg acgcgatcg ccgcaaaat ttcaactac 
        tatctgttc ccgaaagac agtccgttt cgccttaaa 
        ttgttaacg gactcgcaa cgagtggcc ccaggcatg 
        tcggctgaa tttctcgtc gaatttcgc ccccaaact 
        ttccaaaac tgcaccgag tttgtcaaa tttcgcacg 
        atcaagggc gccgatctc agcgtgctt gtgtcaagc 
        gcgcgggat tcccccaaa ctcgcgtgc tacattttc 
        aaccctttc ctgccctgg tacgccgtc cggaagccg 
        gggcccggc agcaagtat ttttcagag cggaggcgc 
        acggcgctc aatttcacg atcgactgc ggtacgtgt 
        ctcttgggg aaccactcg ttggtcacc ctgataatg 
        aaaaatgac ggcgtcaga gggcgcttt ctggtcacg 
        actgaaaac gagtgggtt ttcagcgaa ctcaatgtc 
        cgtgcttgt gagatttcg cacggttgt ttcaccatt 
        ttcccggct tttttcgaa atcgacagc ggcgagatt 
        gggcaaatt gccgtcgct ttcgtgccc gacaaagtc 
        ggggttttc accgaaaaa ttcgtcatc atctgtgac 
        aacaactcg catttggaa attgagatc ctgggcgac 
        tgtattttg tactcggac aagtttctc aaagtcaac 
        atgtatttg ctggaacac actttttcg tacagtaat 
        cggctactt tctataaat ttagcgcct cggcagact 
        tcaagtgtc attttttcg attctgaat aacagtccg 
        gtttatttt aattattac tggttgtta aaaacttca 
        actgacgaa aagtacgct gggaaaaaa atggaccct 
        gaatggata cgaattttg tacggcact cgatttctt 
        ccaactttt agcacaacc gacgttatt gtacagtta 
        acagcggac acggaagaa gagggtttt tattacatt 
        tttttaagt ttttattta aaaaatatc ccgctggta 
        agtctggaa gcctatgac actaaaaca catgtaata 
        aacaactac aaatgtgac tcttcttgc actattgag 
        caagacaat ggtgagaat ttattcacc gattcgcaa 
        ttaggagac gtccacgtc tttgaacta gaaataaac 
        tgcacggtt aaacaacca gaacccgtc aaacttgct 
        acggttaac gtcagtttg gacatcccg acccaagga 
        gtgtcccca cctgtttcc ttgcgccta tcaaaaaga 
        gtcctcaat tttggcata gtcccgaaa ggagtctcg 
        gtttctcaa agcataagt gttgaaaat ttgactgac 
        gatgagatc gagtggaca ataatggaa atccattgc 
        accgacaag tcgtacctt tgcgtctcg tccacagcc 
        ggcgatttg gaccactac agtggcacg ttgaagggg 
        aaacaaaaa aagaaactt cactatacg atcgaagcc 
        aaagacgcc aaacgctgg gtttcaatt ttaatcctt 
        ttcgcgtgc ccttcgtgc acttgccag aagtacacc 
        ctcgaatac atctgtata gtcgcgtac caagtcgcc 
        gaaatcaat atttccctc cgcacagac acccagctc 
        tccgtgctg tgccctcgg gaattcatc tacgtcgaa 
        atccccaca atacacaca atctacgtc cacaattcg 
        ggcctggtg gtgggctgc ttctacttc ctcaacccc 
        gtgggggcc gacgcagac aagctgcgg ctccgattc 
        cgcccccag tcgggggtg gtccagcca ggggccgcg 
        gtcgaggtc agagtcgag ctgaccgcc ctcaaggag 
        ggcctcctg gaccgcgtc cacgtcccc tgcttcgtc 
        ggagatcct gagcgactc attgcctta cgagttctc 
        tgctcagtg gccacgatc aacgtgcat ttctacctc 
        ccggacggg gaccgcggg tacaggagg tacatctgg 
        ccgccgaag agcgaaatg ccggcgtgg gaccacagc 
        atgggcagt cgaggggag ggcgggttt cggggcgat 
        aagggggaa agtgtgtcg gatggcgaa gggggtgac 
        ttaggcgat agtcttttg tctttgatt gggggggat 
        tcagtcgct gggacttac ccgacgatc aagaggagg 
        catgtgggg gtcagggaa gctacaaga gtgtctttc 
        tactttgag aatttaacg ccgattaag gggcgtttt 
        gcggttgag gctgcgaat tacgggcct gcgagcgag 
        tgtttcagt catgttctg gttaaagcc agaactagg 
        accagggaa tttttttgg gagagagct tgcttggac 
        ggttacggc atcgtaatc gagcctgtt taccgctcc 
        ggcgacata atcccctac gaggtggtc gagatcgac 
        ctctggatc tacgccaac acttggggg aagtacctc 
        gaggagata atcgtggaa attggcgac ttgacgccg 
        ttttgcttc aatttaatg gtcgaggtt gtgggaccg 
        ccgattgag tatcccttt gcccgcaac acaacgctg 
        ccgtgtccc actcttcgt tttgaaaac atcgcgtat 
        gcgactgaa agcaccaca acttgtttc actgtcacc 
        aacatcagt gcagttccg attcaaatc agttggcac 
        acgtactac gacaatgaa cataagcca gtgtcagcc 
        cctgtgaat tttcttctc gagtttaat gacgatgag 
        gttcagttt gtaatgtcc cgggattac tacgggtgt 
        gaagcttgt agtaaatgc attatgata gaaccctgg 
        gatttagtt ttggaggcg caagaaact gccacagtt 
        agtgtgaca ataagtgcg tccggtttt aatccaagc 
        tgtgataaa acgaagctc aagtacaca ttgttggga 
        tttattggt ctgagaaac agcgataag tttaaggag 
        aactacttt aaaagatta tcaggagtg gacctcact 
        ccaactaaa gtggaagtg ttagtcacg atggagttg 
        ccgattatg caagtcgat attgcaagt gatgaaacc 
        actattgat atcttcgtt aatgatttg ctcctcaaa 
        aatcataaa ataccgacg gtttatctt cttttgaga 
        aatattgat atgagttcg atcattttt tccatcgag 
        attgagaaa ccgttcaaa attgttgaa gcgcgaact 
        attcccggg ggaatcact gatcaaaaa aacttgatt 
        ctcgagaga cacgaatgt ttacaggtt gcggtaact 
        tgtcgcctc acaagccaa gacttgttc gactattct 
        gaatttatc tacaagtcg gaagacaac agcacagtc 
        gagaactac acgagtgtg atctccaaa gacttgaac 
        gttttccaa aagggggca aagcgccag attcggcga 
        gtgtctcct atttcgcac caagaaaag catgatttc 
        gatttacaa catgacagg agctttaac aagtttcga 
        aattctcaa aacaaccga acatga
      mRNA sequence
      • >TC012646 RA
        aaaatgaag atcacgaat aaatacccc cgaaatgtt 
        gccgaaaac taccgaaaa tacaaagtc gggggcgat 
        tttaccgac cagaaaaac acggctttg gccacagtc 
        aaagtcttc gaggctctt gagccgcgc ttgagcgac 
        atttccgac gtggaagag gagctgatg cgcaaccac 
        gcgctcgct ttggtgcaa aaagaacaa aatttgcca 
        ctcatcgaa cacttgcgc ctcaagaga ttcgacttc 
        ttccaaaaa ctatcagac aatgctgtc aagacttac 
        gagctaatt cagcgaagc gatctgtac ccgaacaaa 
        attgaacag tacttagat ttcacctac aatcctggc 
        ttgcacacg tgcgaccct tgcattggt gaaaaatca 
        aacgaaata aaaaccacc ataaaaaag aaaccacgg 
        aaatgtgag cgaagcgaa cccgacgtg accccacct 
        ttccaggca gcgccccac caaataatt ttctggaac 
        tacgagctc ggtcaagtc tacaggcaa aaattcaaa 
        attattaac acgacgcga tcgccgcaa aatttcaac 
        tactatctg ttcccgaaa gacagtccg tttcgcctt 
        aaattgtta acggactcg caacgagtg gccccaggc 
        atgtcggct gaatttctc gtcgaattt cgcccccaa 
        actttccaa aactgcacc gagtttgtc aaatttcgc 
        acgatcaag ggcgccgat ctcagcgtg cttgtgtca 
        agcgcgcgg gattccccc aaactcgcg tgctacatt 
        ttcaaccct ttcctgccc tggtacgcc gtccggaag 
        ccggggccc ggcagcaag tatttttca gagcggagg 
        cgcacggcg ctcaatttc acgatcgac tgcggtacg 
        tgtctcttg gggaaccac tcgttggtc accctgata 
        atgaaaaat gacggcgtc agagggcgc tttctggtc 
        acgactgaa aacgagtgg gttttcagc gaactcaat 
        gtccgtgct tgtgagatt tcgcacggt tgtttcacc 
        attttcccg gcttttttc gaaatcgac agcggcgag 
        attgggcaa attgccgtc gctttcgtg cccgacaaa 
        gtcggggtt ttcaccgaa aaattcgtc atcatctgt 
        gacaacaac tcgcatttg gaaattgag atcctgggc 
        gactgtatt ttgtactcg gacaagttt ctcaaagtc 
        aacatgtat ttgctggaa cacactttt tcgtacagt 
        aatcggcta ctttctata aatttagcg cctcggcag 
        acttcaagt gtcattttt tcgattctg aataacagt 
        ccggtttat tttaattat tactggttg ttaaaaact 
        tcaactgac gaaaagtac gctgggaaa aaaatggac 
        cctgaatgg atacgaatt ttgtacggc actcgattt 
        cttccaact tttagcaca accgacgtt attgtacag 
        ttaacagcg gacacggaa gaagagggt ttttattac 
        attttttta agtttttat ttaaaaaat atcccgctg 
        gtaagtctg gaagcctat gacactaaa acacatgta 
        ataaacaac tacaaatgt gactcttct tgcactatt 
        gagcaagac aatggtgag aatttattc accgattcg 
        caattagga gacgtccac gtctttgaa ctagaaata 
        aactgcacg gttaaacaa ccagaaccc gtcaaactt 
        gctacggtt aacgtcagt ttggacatc ccgacccaa 
        ggagtgtcc ccacctgtt tccttgcgc ctatcaaaa 
        agagtcctc aattttggc atagtcccg aaaggagtc 
        tcggtttct caaagcata agtgttgaa aatttgact 
        gacgatgag atcgagtgg acaataatg gaaatccat 
        tgcaccgac aagtcgtac ctttgcgtc tcgtccaca 
        gccggcgat ttggaccac tacagtggc acgttgaag 
        gggaaacaa aaaaagaaa cttcactat acgatcgaa 
        gccaaagac gccaaacgc tgggtttca attttaatc 
        cttttcgcg tgcccttcg tgcacttgc cagaagtac 
        accctcgaa tacatctgt atagtcgcg taccaagtc 
        gccgaaatc aatatttcc ctccgcaca gacacccag 
        ctctccgtg ctgtgccct cgggaattc atctacgtc 
        gaaatcccc acaatacac acaatctac gtccacaat 
        tcgggcctg gtggtgggc tgcttctac ttcctcaac 
        cccgtgggg gccgacgca gacaagctg cggctccga 
        ttccgcccc cagtcgggg gtggtccag ccaggggcc 
        gcggtcgag gtcagagtc gagctgacc gccctcaag 
        gagggcctc ctggaccgc gtccacgtc ccctgcttc 
        gtcggagat cctgagcga ctcattgcc ttacgagtt 
        ctctgctca gtggccacg atcaacgtg catttctac 
        ctcccggac ggggaccgc gggtacagg aggtacatc 
        tggccgccg aagagcgaa atgccggcg tgggaccac 
        agcatgggc agtcgaggg gagggcggg tttcggggc 
        gataagggg gaaagtgtg tcggatggc gaagggggt 
        gacttaggc gatagtctt ttgtctttg attgggggg 
        gattcagtc gctgggact tacccgacg atcaagagg 
        aggcatgtg ggggtcagg gaagctaca agagtgtct 
        ttctacttt gagaattta acgccgatt aaggggcgt 
        tttgcggtt gaggctgcg aattacggg cctgcgagc 
        gagtgtttc agtcatgtt ctggttaaa gccagaact 
        aggaccagg gaatttttt tgggagaga gcttgcttg 
        gacggttac ggcatcgta atcgagcct gtttaccgc 
        tccggcgac ataatcccc tacgaggtg gtcgagatc 
        gacctctgg atctacgcc aacacttgg gggaagtac 
        ctcgaggag ataatcgtg gaaattggc gacttgacg 
        ccgttttgc ttcaattta atggtcgag gttgtggga 
        ccgccgatt gagtatccc tttgcccgc aacacaacg 
        ctgccgtgt cccactctt cgttttgaa aacatcgcg 
        tatgcgact gaaagcacc acaacttgt ttcactgtc 
        accaacatc agtgcagtt ccgattcaa atcagttgg 
        cacacgtac tacgacaat gaacataag ccagtgtca 
        gcccctgtg aattttctt ctcgagttt aatgacgat 
        gaggttcag tttgtaatg tcccgggat tactacggg 
        tgtgaagct tgtagtaaa tgcattatg atagaaccc 
        tgggattta gttttggag gcgcaagaa actgccaca 
        gttagtgtg acaataagt gcgtccggt tttaatcca 
        agctgtgat aaaacgaag ctcaagtac acattgttg 
        ggatttatt ggtctgaga aacagcgat aagtttaag 
        gagaactac tttaaaaga ttatcagga gtggacctc 
        actccaact aaagtggaa gtgttagtc acgatggag 
        ttgccgatt atgcaagtc gatattgca agtgatgaa 
        accactatt gatatcttc gttaatgat ttgctcctc 
        aaaaatcat aaaataccg acggtttat cttcttttg 
        agaaatatt gatatgagt tcgatcatt ttttccatc 
        gagattgag aaaccgttc aaaattgtt gaagcgcga 
        actattccc gggggaatc actgatcaa aaaaacttg 
        attctcgag agacacgaa tgtttacag gttgcggta 
        acttgtcgc ctcacaagc caagacttg ttcgactat 
        tctgaattt atctacaag tcggaagac aacagcaca 
        gtcgagaac tacacgagt gtgatctcc aaagacttg 
        aacgttttc caaaagggg gcaaagcgc cagattcgg 
        cgagtgtct cctatttcg caccaagaa aagcatgat 
        ttcgattta caacatgac aggagcttt aacaagttt 
        cgaaattct caaaacaac cgaacatga cgactttga 
        ggtccaccc aagttgtgc ccatcttga ggcaaacac 
        tggagttaa taagcagtt tgtggacgt cgccgtgta 
        tttcactcc cagacattg caaaacgta caacgcaaa 
        tttgcggat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY