Status
Please wait ...
Gene information
General information
TC031194
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013272 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 5957212..5963195
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031194
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07295

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    metathorax constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment random constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031194 RA
      MSISPDAYR PQKSLDANK YATKKTIAQ 
GLLDVALLT ANASQLKYV LQVGEKHEF 
YVLMLTLIS ISIVLQVFQ AILCVLLGT 
TLNLNKEGD HKSADRANN VILCINIIL 
MATNILISA FEMKEDSNK P
    • >TC031194 RB
      MSISPDAYR PQKSLDANK YATKKTIAQ 
GLLDVALLT ANASQLKYV LQVGEKHEF 
YVLMLTLIS ISIVLQVLV GLLCLTLHL 
MRDCRLHLA DYHFSANFL NYITTIFAF 
VITVLNLLI SCFDPTIAN YAVFDPPAL 
DPV
    • >TC031194 RC
      MASENKAGR SQTDGNTIS DYILKSLDA 
NKYATKKTI AQGLLDVAL LTANASQLK 
YVLQVGEKH EFYVLMLTL ISISIVLQV 
LVGLLCLTL HLMRDCRLH LADYHFSAN 
FLNYITTIF AFVITVLNL LISCFDPTI 
ANYAVFDPP ALDPV
    CDS sequence
    • >TC031194 RA
      atgtctatt tctccagat gcttatcgt ccccagaaa 
tccttggac gcaaataaa tacgctaca aagaagacc 
atagcgcaa ggactcctc gacgttgcc ttactcact 
gccaatgcc tcccagctg aagtacgtc ctccaagtc 
ggggaaaaa cacgagttc tacgtattg atgctaacc 
ctaatcagc atatccata gttctgcag gtgttccaa 
gcgatcctg tgtgttctt ctggggact acacttaat 
ctcaacaaa gaaggggat cacaagtct gcagacaga 
gccaacaat gttatactc tgcattaat attatccta 
atggctaca aatattcta atcagtgca tttgaaatg 
aaggaggat agcaataaa ccgtga
    • >TC031194 RB
      atgtctatt tctccagat gcttatcgt ccccagaaa 
tccttggac gcaaataaa tacgctaca aagaagacc 
atagcgcaa ggactcctc gacgttgcc ttactcact 
gccaatgcc tcccagctg aagtacgtc ctccaagtc 
ggggaaaaa cacgagttc tacgtattg atgctaacc 
ctaatcagc atatccata gttctgcag gtgctcgtg 
gggctcctt tgtctcaca cttcattta atgagggac 
tgtcggttg cacctcgcg gactaccac ttttccgcc 
aactttctt aactatata accacaatt ttcgctttt 
gttataaca gttttgaat ttgcttatt tcgtgtttc 
gaccccact atagcaaat tacgcagtt tttgatccc 
ccagcgctg gatcctgtg taa
    • >TC031194 RC
      atggcaagt gaaaataaa gctggtaga tcccaaact 
gatgggaat accatcagt gattatatc ctgaaatcc 
ttggacgca aataaatac gctacaaag aagaccata 
gcgcaagga ctcctcgac gttgcctta ctcactgcc 
aatgcctcc cagctgaag tacgtcctc caagtcggg 
gaaaaacac gagttctac gtattgatg ctaacccta 
atcagcata tccatagtt ctgcaggtg ctcgtgggg 
ctcctttgt ctcacactt catttaatg agggactgt 
cggttgcac ctcgcggac taccacttt tccgccaac 
tttcttaac tatataacc acaattttc gcttttgtt 
ataacagtt ttgaatttg cttatttcg tgtttcgac 
cccactata gcaaattac gcagttttt gatccccca 
gcgctggat cctgtgtaa
    mRNA sequence
    • >TC031194 RA
      taaaagtcg agataatgc aacgagtgg gagacagtt 
tgctcaaga ttagaagtc caccacatt ctcttatcc 
gccttggat gtttctgtc ttctgtgcc gttttgaac 
cttttgtca cctcgccac gacttcatg agtcagtca 
ctcaatacg tttaatgtt tgcattgaa agccaaaat 
tgttgcaat tacgacgac gggtgcgtc actaccatt 
ggtcgagga aaattgatc gtggtagag cttattccc 
gcggcattg cctggaagg ttgttttaa tcgaaagtc 
gacggatta acgttatac caagcatta tgttttatc 
aacgtttca ctcactatc tcctctaga caattgtac 
aagtgcttt tgttttcgt ttgatcgat atatcttgt 
ccaggtaga aaaatgtct atttctcca gatgcttat 
cgtccccag aaatccttg gacgcaaat aaatacgct 
acaaagaag accatagcg caaggactc ctcgacgtt 
gccttactc actgccaat gcctcccag ctgaagtac 
gtcctccaa gtcggggaa aaacacgag ttctacgta 
ttgatgcta accctaatc agcatatcc atagttctg 
caggtgttc caagcgatc ctgtgtgtt cttctgggg 
actacactt aatctcaac aaagaaggg gatcacaag 
tctgcagac agagccaac aatgttata ctctgcatt 
aatattatc ctaatggct acaaatatt ctaatcagt 
gcatttgaa atgaaggag gatagcaat aaaccgtga 
ttagtgttt aagtgacag cataatgta tttatttat 
ggaataggt ttataaaat catgtcaaa agggactat 
caagttgag taagattta catttagga ttatgtgta 
aaaagaaca tcgggatgc attgtagga tactctctt 
agcctattt tttgtgtat tattcaagt gcatttacc 
tagtgatat ctttaatag tcagatata tttatatta 
attaaataa aagtatttc caaagacac ttctatatt 
tagtacgta atcaattaa ataaattgt tataatgag 
a
    • >TC031194 RB
      taaaagtcg agataatgc aacgagtgg gagacagtt 
tgctcaaga ttagaagtc caccacatt ctcttatcc 
gccttggat gtttctgtc ttctgtgcc gttttgaac 
cttttgtca cctcgccac gacttcatg agtcagtca 
ctcaatacg tttaatgtt tgcattgaa agccaaaat 
tgttgcaat tacgacgac gggtgcgtc actaccatt 
ggtcgagga aaattgatc gtggtagag cttattccc 
gcggcattg cctggaagg ttgttttaa tcgaaagtc 
gacggatta acgttatac caagcatta tgttttatc 
aacgtttca ctcactatc tcctctaga caattgtac 
aagtgcttt tgttttcgt ttgatcgat atatcttgt 
ccaggtaga aaaatgtct atttctcca gatgcttat 
cgtccccag aaatccttg gacgcaaat aaatacgct 
acaaagaag accatagcg caaggactc ctcgacgtt 
gccttactc actgccaat gcctcccag ctgaagtac 
gtcctccaa gtcggggaa aaacacgag ttctacgta 
ttgatgcta accctaatc agcatatcc atagttctg 
caggtgctc gtggggctc ctttgtctc acacttcat 
ttaatgagg gactgtcgg ttgcacctc gcggactac 
cacttttcc gccaacttt cttaactat ataaccaca 
attttcgct tttgttata acagttttg aatttgctt 
atttcgtgt ttcgacccc actatagca aattacgca 
gtttttgat cccccagcg ctggatcct gtgtaaaaa 
tattttttt ctttcagtt aatcgtcgc tattttgtt 
cgtgattat tggaggcct caacattaa caagcagcg 
ggaccattt cgccgctta tgtcctaaa tgacgttat 
tctcatttt aattttttt gatttccgt tattaatat 
tgttatttc cgggttcgg gctcgaata ctcaaatca 
accacttag attaattta cgaacatac cgaagctcc 
gcctcgctc gtaattaga atatgtcgc caaatactt 
atttctgtt gttttacgt tccttgcct aacaccatt 
atttttctt taggtgaca gcagccttg ctctcaatc 
gtggtttca ataatgcaa acatacgag gatgaaaaa 
attaaaaac ctaagttgg atattacat catgtttct 
ttaggtttc gtgaccggg gcgttattt tgcgatata 
atcaaaatg aattttggg ttggatccc gccgttgct 
gttaacgtt actactctt aaaactgaa ctttgattt 
tttacttta tttttcttt atttttttt tattttttt 
tttattttt tttcgtgac actaaggtg ttccaagcg 
atcctgtgt gttcttctg gggactaca cttaatctc 
aacaaagaa ggggatcac aagtctgca gacagagcc 
aacaatgtt atactctgc attaatatt atcctaatg 
gctacaaat attctaatc agtgcattt gaaatgaag 
gaggatagc aataaaccg tgattagtg tttaagtga 
cagcataat gtatttatt tatggaata ggtttataa 
aatcatgtc aaaagggac tatcaagtt gagtaagat 
ttacattta ggattatgt gtaaaaaga acatcggga 
tgcattgta ggatactct cttagccta ttttttgtg 
tattattca agtgcattt acctagtga tatctttaa 
tagtcagat atatttata ttaattaaa taaaagtat 
ttccaaaga cacttctat atttagtac gtaatcaat 
taaataaat tgttataat gaga
    • >TC031194 RC
      taaaagtcg agataatgc aacgagtgg gagacagtt 
tgctcaaga ttagagtaa taacatttc gtattgctc 
ttgtggtta atagctttg acattattt gcaatttgc 
agagtccac cacattctc ttatccgcc ttggatgtt 
tctgtcttc tgtgccgtt ttgaacctt ttgtcacct 
cgccacgac ttcatgagt cagtcactc aatacgttt 
aatgtttgc attgaaagc caaaattgt tgcaattac 
gacgacggg tgcgtcact accattggt cgaggaaaa 
ttgatcgtg gtagagctt attcccgcg gcattgcct 
ggaaggttg ttttaatcg aaagtcgac ggattaacg 
ttataccaa gcattatgt tttatcaac gtttcactc 
actatctcc tctagacaa ttgtacaag tgcttttgt 
tttcgtttg atcgatata tcttgtcca ggtagaaaa 
atgtctatt tctccagat gcttatcgt ccccagtga 
ttcgtgacc actctgtaa caatggcaa gtgaaaata 
aagctggta gatcccaaa ctgatggga ataccatca 
gtgattata tcctgaaat ccttggacg caaataaat 
acgctacaa agaagacca tagcgcaag gactcctcg 
acgttgcct tactcactg ccaatgcct cccagctga 
agtacgtcc tccaagtcg gggaaaaac acgagttct 
acgtattga tgctaaccc taatcagca tatccatag 
ttctgcagg tgctcgtgg ggctccttt gtctcacac 
ttcatttaa tgagggact gtcggttgc acctcgcgg 
actaccact tttccgcca actttctta actatataa 
ccacaattt tcgcttttg ttataacag ttttgaatt 
tgcttattt cgtgtttcg accccacta tagcaaatt 
acgcagttt ttgatcccc cagcgctgg atcctgtgt 
aaaaatatt tttttcttt cagttaatc gtcgctatt 
ttgttcgtg attattgga ggcctcaac attaacaag 
cagcgggac catttcgcc gcttatgtc ctaaatgac 
gttattctc attttaatt tttttgatt tccgttatt 
aatattgtt atttccggg ttcgggctc gaatactca 
aatcaacca cttagatta atttacgaa cataccgaa 
gctccgcct cgctcgtaa ttagaatat gtcgccaaa 
tacttattt ctgttgttt tacgttcct tgcctaaca 
ccattattt ttctttagg tgacagcag ccttgctct 
caatcgtgg tttcaataa tgcaaacat acgaggatg 
aaaaaatta aaaacctaa gttggatat tacatcatg 
tttctttag gtttcgtga ccggggcgt tattttgcg 
atataatca aaatgaatt ttgggttgg atcccgccg 
ttgctgtta acgttacta ctcttaaaa ctgaacttt 
gatttttta ctttatttt tctttattt ttttttatt 
tttttttta ttttttttc gtgacacta aggtgttcc 
aagcgatcc tgtgtgttc ttctgggga ctacactta 
atctcaaca aagaagggg atcacaagt ctgcagaca 
gagccaaca atgttatac tctgcatta atattatcc 
taatggcta caaatattc taatcagtg catttgaaa 
tgaaggagg atagcaata aaccgtgat tagtgttta 
agtgacagc ataatgtat ttatttatg gaataggtt 
tataaaatc atgtcaaaa gggactatc aagttgagt 
aagatttac atttaggat tatgtgtaa aaagaacat 
cgggatgca ttgtaggat actctctta gcctatttt 
ttgtgtatt attcaagtg catttacct agtgatatc 
tttaatagt cagatatat ttatattaa ttaaataaa 
agtatttcc aaagacact tctatattt agtacgtaa 
tcaattaaa taaattgtt ataatgaga

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY