Status
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Gene information
General information
TC031194
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013272
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 5957212..5963195
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031194
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07295

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    metathorax constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment random constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031194 RA
      MSISPDAYR PQKSLDANK YATKKTIAQ 
      GLLDVALLT ANASQLKYV LQVGEKHEF 
      YVLMLTLIS ISIVLQVFQ AILCVLLGT 
      TLNLNKEGD HKSADRANN VILCINIIL 
      MATNILISA FEMKEDSNK P
    • >TC031194 RB
      MSISPDAYR PQKSLDANK YATKKTIAQ 
      GLLDVALLT ANASQLKYV LQVGEKHEF 
      YVLMLTLIS ISIVLQVLV GLLCLTLHL 
      MRDCRLHLA DYHFSANFL NYITTIFAF 
      VITVLNLLI SCFDPTIAN YAVFDPPAL 
      DPV
    • >TC031194 RC
      MASENKAGR SQTDGNTIS DYILKSLDA 
      NKYATKKTI AQGLLDVAL LTANASQLK 
      YVLQVGEKH EFYVLMLTL ISISIVLQV 
      LVGLLCLTL HLMRDCRLH LADYHFSAN 
      FLNYITTIF AFVITVLNL LISCFDPTI 
      ANYAVFDPP ALDPV
    CDS sequence
    • >TC031194 RA
      atgtctatt tctccagat gcttatcgt ccccagaaa 
      tccttggac gcaaataaa tacgctaca aagaagacc 
      atagcgcaa ggactcctc gacgttgcc ttactcact 
      gccaatgcc tcccagctg aagtacgtc ctccaagtc 
      ggggaaaaa cacgagttc tacgtattg atgctaacc 
      ctaatcagc atatccata gttctgcag gtgttccaa 
      gcgatcctg tgtgttctt ctggggact acacttaat 
      ctcaacaaa gaaggggat cacaagtct gcagacaga 
      gccaacaat gttatactc tgcattaat attatccta 
      atggctaca aatattcta atcagtgca tttgaaatg 
      aaggaggat agcaataaa ccgtga
    • >TC031194 RB
      atgtctatt tctccagat gcttatcgt ccccagaaa 
      tccttggac gcaaataaa tacgctaca aagaagacc 
      atagcgcaa ggactcctc gacgttgcc ttactcact 
      gccaatgcc tcccagctg aagtacgtc ctccaagtc 
      ggggaaaaa cacgagttc tacgtattg atgctaacc 
      ctaatcagc atatccata gttctgcag gtgctcgtg 
      gggctcctt tgtctcaca cttcattta atgagggac 
      tgtcggttg cacctcgcg gactaccac ttttccgcc 
      aactttctt aactatata accacaatt ttcgctttt 
      gttataaca gttttgaat ttgcttatt tcgtgtttc 
      gaccccact atagcaaat tacgcagtt tttgatccc 
      ccagcgctg gatcctgtg taa
    • >TC031194 RC
      atggcaagt gaaaataaa gctggtaga tcccaaact 
      gatgggaat accatcagt gattatatc ctgaaatcc 
      ttggacgca aataaatac gctacaaag aagaccata 
      gcgcaagga ctcctcgac gttgcctta ctcactgcc 
      aatgcctcc cagctgaag tacgtcctc caagtcggg 
      gaaaaacac gagttctac gtattgatg ctaacccta 
      atcagcata tccatagtt ctgcaggtg ctcgtgggg 
      ctcctttgt ctcacactt catttaatg agggactgt 
      cggttgcac ctcgcggac taccacttt tccgccaac 
      tttcttaac tatataacc acaattttc gcttttgtt 
      ataacagtt ttgaatttg cttatttcg tgtttcgac 
      cccactata gcaaattac gcagttttt gatccccca 
      gcgctggat cctgtgtaa
    mRNA sequence
    • >TC031194 RA
      taaaagtcg agataatgc aacgagtgg gagacagtt 
      tgctcaaga ttagaagtc caccacatt ctcttatcc 
      gccttggat gtttctgtc ttctgtgcc gttttgaac 
      cttttgtca cctcgccac gacttcatg agtcagtca 
      ctcaatacg tttaatgtt tgcattgaa agccaaaat 
      tgttgcaat tacgacgac gggtgcgtc actaccatt 
      ggtcgagga aaattgatc gtggtagag cttattccc 
      gcggcattg cctggaagg ttgttttaa tcgaaagtc 
      gacggatta acgttatac caagcatta tgttttatc 
      aacgtttca ctcactatc tcctctaga caattgtac 
      aagtgcttt tgttttcgt ttgatcgat atatcttgt 
      ccaggtaga aaaatgtct atttctcca gatgcttat 
      cgtccccag aaatccttg gacgcaaat aaatacgct 
      acaaagaag accatagcg caaggactc ctcgacgtt 
      gccttactc actgccaat gcctcccag ctgaagtac 
      gtcctccaa gtcggggaa aaacacgag ttctacgta 
      ttgatgcta accctaatc agcatatcc atagttctg 
      caggtgttc caagcgatc ctgtgtgtt cttctgggg 
      actacactt aatctcaac aaagaaggg gatcacaag 
      tctgcagac agagccaac aatgttata ctctgcatt 
      aatattatc ctaatggct acaaatatt ctaatcagt 
      gcatttgaa atgaaggag gatagcaat aaaccgtga 
      ttagtgttt aagtgacag cataatgta tttatttat 
      ggaataggt ttataaaat catgtcaaa agggactat 
      caagttgag taagattta catttagga ttatgtgta 
      aaaagaaca tcgggatgc attgtagga tactctctt 
      agcctattt tttgtgtat tattcaagt gcatttacc 
      tagtgatat ctttaatag tcagatata tttatatta 
      attaaataa aagtatttc caaagacac ttctatatt 
      tagtacgta atcaattaa ataaattgt tataatgag 
      a
    • >TC031194 RB
      taaaagtcg agataatgc aacgagtgg gagacagtt 
      tgctcaaga ttagaagtc caccacatt ctcttatcc 
      gccttggat gtttctgtc ttctgtgcc gttttgaac 
      cttttgtca cctcgccac gacttcatg agtcagtca 
      ctcaatacg tttaatgtt tgcattgaa agccaaaat 
      tgttgcaat tacgacgac gggtgcgtc actaccatt 
      ggtcgagga aaattgatc gtggtagag cttattccc 
      gcggcattg cctggaagg ttgttttaa tcgaaagtc 
      gacggatta acgttatac caagcatta tgttttatc 
      aacgtttca ctcactatc tcctctaga caattgtac 
      aagtgcttt tgttttcgt ttgatcgat atatcttgt 
      ccaggtaga aaaatgtct atttctcca gatgcttat 
      cgtccccag aaatccttg gacgcaaat aaatacgct 
      acaaagaag accatagcg caaggactc ctcgacgtt 
      gccttactc actgccaat gcctcccag ctgaagtac 
      gtcctccaa gtcggggaa aaacacgag ttctacgta 
      ttgatgcta accctaatc agcatatcc atagttctg 
      caggtgctc gtggggctc ctttgtctc acacttcat 
      ttaatgagg gactgtcgg ttgcacctc gcggactac 
      cacttttcc gccaacttt cttaactat ataaccaca 
      attttcgct tttgttata acagttttg aatttgctt 
      atttcgtgt ttcgacccc actatagca aattacgca 
      gtttttgat cccccagcg ctggatcct gtgtaaaaa 
      tattttttt ctttcagtt aatcgtcgc tattttgtt 
      cgtgattat tggaggcct caacattaa caagcagcg 
      ggaccattt cgccgctta tgtcctaaa tgacgttat 
      tctcatttt aattttttt gatttccgt tattaatat 
      tgttatttc cgggttcgg gctcgaata ctcaaatca 
      accacttag attaattta cgaacatac cgaagctcc 
      gcctcgctc gtaattaga atatgtcgc caaatactt 
      atttctgtt gttttacgt tccttgcct aacaccatt 
      atttttctt taggtgaca gcagccttg ctctcaatc 
      gtggtttca ataatgcaa acatacgag gatgaaaaa 
      attaaaaac ctaagttgg atattacat catgtttct 
      ttaggtttc gtgaccggg gcgttattt tgcgatata 
      atcaaaatg aattttggg ttggatccc gccgttgct 
      gttaacgtt actactctt aaaactgaa ctttgattt 
      tttacttta tttttcttt atttttttt tattttttt 
      tttattttt tttcgtgac actaaggtg ttccaagcg 
      atcctgtgt gttcttctg gggactaca cttaatctc 
      aacaaagaa ggggatcac aagtctgca gacagagcc 
      aacaatgtt atactctgc attaatatt atcctaatg 
      gctacaaat attctaatc agtgcattt gaaatgaag 
      gaggatagc aataaaccg tgattagtg tttaagtga 
      cagcataat gtatttatt tatggaata ggtttataa 
      aatcatgtc aaaagggac tatcaagtt gagtaagat 
      ttacattta ggattatgt gtaaaaaga acatcggga 
      tgcattgta ggatactct cttagccta ttttttgtg 
      tattattca agtgcattt acctagtga tatctttaa 
      tagtcagat atatttata ttaattaaa taaaagtat 
      ttccaaaga cacttctat atttagtac gtaatcaat 
      taaataaat tgttataat gaga
    • >TC031194 RC
      taaaagtcg agataatgc aacgagtgg gagacagtt 
      tgctcaaga ttagagtaa taacatttc gtattgctc 
      ttgtggtta atagctttg acattattt gcaatttgc 
      agagtccac cacattctc ttatccgcc ttggatgtt 
      tctgtcttc tgtgccgtt ttgaacctt ttgtcacct 
      cgccacgac ttcatgagt cagtcactc aatacgttt 
      aatgtttgc attgaaagc caaaattgt tgcaattac 
      gacgacggg tgcgtcact accattggt cgaggaaaa 
      ttgatcgtg gtagagctt attcccgcg gcattgcct 
      ggaaggttg ttttaatcg aaagtcgac ggattaacg 
      ttataccaa gcattatgt tttatcaac gtttcactc 
      actatctcc tctagacaa ttgtacaag tgcttttgt 
      tttcgtttg atcgatata tcttgtcca ggtagaaaa 
      atgtctatt tctccagat gcttatcgt ccccagtga 
      ttcgtgacc actctgtaa caatggcaa gtgaaaata 
      aagctggta gatcccaaa ctgatggga ataccatca 
      gtgattata tcctgaaat ccttggacg caaataaat 
      acgctacaa agaagacca tagcgcaag gactcctcg 
      acgttgcct tactcactg ccaatgcct cccagctga 
      agtacgtcc tccaagtcg gggaaaaac acgagttct 
      acgtattga tgctaaccc taatcagca tatccatag 
      ttctgcagg tgctcgtgg ggctccttt gtctcacac 
      ttcatttaa tgagggact gtcggttgc acctcgcgg 
      actaccact tttccgcca actttctta actatataa 
      ccacaattt tcgcttttg ttataacag ttttgaatt 
      tgcttattt cgtgtttcg accccacta tagcaaatt 
      acgcagttt ttgatcccc cagcgctgg atcctgtgt 
      aaaaatatt tttttcttt cagttaatc gtcgctatt 
      ttgttcgtg attattgga ggcctcaac attaacaag 
      cagcgggac catttcgcc gcttatgtc ctaaatgac 
      gttattctc attttaatt tttttgatt tccgttatt 
      aatattgtt atttccggg ttcgggctc gaatactca 
      aatcaacca cttagatta atttacgaa cataccgaa 
      gctccgcct cgctcgtaa ttagaatat gtcgccaaa 
      tacttattt ctgttgttt tacgttcct tgcctaaca 
      ccattattt ttctttagg tgacagcag ccttgctct 
      caatcgtgg tttcaataa tgcaaacat acgaggatg 
      aaaaaatta aaaacctaa gttggatat tacatcatg 
      tttctttag gtttcgtga ccggggcgt tattttgcg 
      atataatca aaatgaatt ttgggttgg atcccgccg 
      ttgctgtta acgttacta ctcttaaaa ctgaacttt 
      gatttttta ctttatttt tctttattt ttttttatt 
      tttttttta ttttttttc gtgacacta aggtgttcc 
      aagcgatcc tgtgtgttc ttctgggga ctacactta 
      atctcaaca aagaagggg atcacaagt ctgcagaca 
      gagccaaca atgttatac tctgcatta atattatcc 
      taatggcta caaatattc taatcagtg catttgaaa 
      tgaaggagg atagcaata aaccgtgat tagtgttta 
      agtgacagc ataatgtat ttatttatg gaataggtt 
      tataaaatc atgtcaaaa gggactatc aagttgagt 
      aagatttac atttaggat tatgtgtaa aaagaacat 
      cgggatgca ttgtaggat actctctta gcctatttt 
      ttgtgtatt attcaagtg catttacct agtgatatc 
      tttaatagt cagatatat ttatattaa ttaaataaa 
      agtatttcc aaagacact tctatattt agtacgtaa 
      tcaattaaa taaattgtt ataatgaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY