General information | |||
TC031194
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 5957212..5963195 | |
Sequence information | |||
Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC031194 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07295 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
metathorax constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment random constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031194 RA
MSISPDAYR PQKSLDANK YATKKTIAQ GLLDVALLT ANASQLKYV LQVGEKHEF YVLMLTLIS ISIVLQVFQ AILCVLLGT TLNLNKEGD HKSADRANN VILCINIIL MATNILISA FEMKEDSNK P
- >TC031194 RB
MSISPDAYR PQKSLDANK YATKKTIAQ GLLDVALLT ANASQLKYV LQVGEKHEF YVLMLTLIS ISIVLQVLV GLLCLTLHL MRDCRLHLA DYHFSANFL NYITTIFAF VITVLNLLI SCFDPTIAN YAVFDPPAL DPV
- >TC031194 RC
MASENKAGR SQTDGNTIS DYILKSLDA NKYATKKTI AQGLLDVAL LTANASQLK YVLQVGEKH EFYVLMLTL ISISIVLQV LVGLLCLTL HLMRDCRLH LADYHFSAN FLNYITTIF AFVITVLNL LISCFDPTI ANYAVFDPP ALDPV
- >TC031194 RA
atgtctatt tctccagat gcttatcgt ccccagaaa tccttggac gcaaataaa tacgctaca aagaagacc atagcgcaa ggactcctc gacgttgcc ttactcact gccaatgcc tcccagctg aagtacgtc ctccaagtc ggggaaaaa cacgagttc tacgtattg atgctaacc ctaatcagc atatccata gttctgcag gtgttccaa gcgatcctg tgtgttctt ctggggact acacttaat ctcaacaaa gaaggggat cacaagtct gcagacaga gccaacaat gttatactc tgcattaat attatccta atggctaca aatattcta atcagtgca tttgaaatg aaggaggat agcaataaa ccgtga
- >TC031194 RB
atgtctatt tctccagat gcttatcgt ccccagaaa tccttggac gcaaataaa tacgctaca aagaagacc atagcgcaa ggactcctc gacgttgcc ttactcact gccaatgcc tcccagctg aagtacgtc ctccaagtc ggggaaaaa cacgagttc tacgtattg atgctaacc ctaatcagc atatccata gttctgcag gtgctcgtg gggctcctt tgtctcaca cttcattta atgagggac tgtcggttg cacctcgcg gactaccac ttttccgcc aactttctt aactatata accacaatt ttcgctttt gttataaca gttttgaat ttgcttatt tcgtgtttc gaccccact atagcaaat tacgcagtt tttgatccc ccagcgctg gatcctgtg taa
- >TC031194 RC
atggcaagt gaaaataaa gctggtaga tcccaaact gatgggaat accatcagt gattatatc ctgaaatcc ttggacgca aataaatac gctacaaag aagaccata gcgcaagga ctcctcgac gttgcctta ctcactgcc aatgcctcc cagctgaag tacgtcctc caagtcggg gaaaaacac gagttctac gtattgatg ctaacccta atcagcata tccatagtt ctgcaggtg ctcgtgggg ctcctttgt ctcacactt catttaatg agggactgt cggttgcac ctcgcggac taccacttt tccgccaac tttcttaac tatataacc acaattttc gcttttgtt ataacagtt ttgaatttg cttatttcg tgtttcgac cccactata gcaaattac gcagttttt gatccccca gcgctggat cctgtgtaa
- >TC031194 RA
taaaagtcg agataatgc aacgagtgg gagacagtt tgctcaaga ttagaagtc caccacatt ctcttatcc gccttggat gtttctgtc ttctgtgcc gttttgaac cttttgtca cctcgccac gacttcatg agtcagtca ctcaatacg tttaatgtt tgcattgaa agccaaaat tgttgcaat tacgacgac gggtgcgtc actaccatt ggtcgagga aaattgatc gtggtagag cttattccc gcggcattg cctggaagg ttgttttaa tcgaaagtc gacggatta acgttatac caagcatta tgttttatc aacgtttca ctcactatc tcctctaga caattgtac aagtgcttt tgttttcgt ttgatcgat atatcttgt ccaggtaga aaaatgtct atttctcca gatgcttat cgtccccag aaatccttg gacgcaaat aaatacgct acaaagaag accatagcg caaggactc ctcgacgtt gccttactc actgccaat gcctcccag ctgaagtac gtcctccaa gtcggggaa aaacacgag ttctacgta ttgatgcta accctaatc agcatatcc atagttctg caggtgttc caagcgatc ctgtgtgtt cttctgggg actacactt aatctcaac aaagaaggg gatcacaag tctgcagac agagccaac aatgttata ctctgcatt aatattatc ctaatggct acaaatatt ctaatcagt gcatttgaa atgaaggag gatagcaat aaaccgtga ttagtgttt aagtgacag cataatgta tttatttat ggaataggt ttataaaat catgtcaaa agggactat caagttgag taagattta catttagga ttatgtgta aaaagaaca tcgggatgc attgtagga tactctctt agcctattt tttgtgtat tattcaagt gcatttacc tagtgatat ctttaatag tcagatata tttatatta attaaataa aagtatttc caaagacac ttctatatt tagtacgta atcaattaa ataaattgt tataatgag a
- >TC031194 RB
taaaagtcg agataatgc aacgagtgg gagacagtt tgctcaaga ttagaagtc caccacatt ctcttatcc gccttggat gtttctgtc ttctgtgcc gttttgaac cttttgtca cctcgccac gacttcatg agtcagtca ctcaatacg tttaatgtt tgcattgaa agccaaaat tgttgcaat tacgacgac gggtgcgtc actaccatt ggtcgagga aaattgatc gtggtagag cttattccc gcggcattg cctggaagg ttgttttaa tcgaaagtc gacggatta acgttatac caagcatta tgttttatc aacgtttca ctcactatc tcctctaga caattgtac aagtgcttt tgttttcgt ttgatcgat atatcttgt ccaggtaga aaaatgtct atttctcca gatgcttat cgtccccag aaatccttg gacgcaaat aaatacgct acaaagaag accatagcg caaggactc ctcgacgtt gccttactc actgccaat gcctcccag ctgaagtac gtcctccaa gtcggggaa aaacacgag ttctacgta ttgatgcta accctaatc agcatatcc atagttctg caggtgctc gtggggctc ctttgtctc acacttcat ttaatgagg gactgtcgg ttgcacctc gcggactac cacttttcc gccaacttt cttaactat ataaccaca attttcgct tttgttata acagttttg aatttgctt atttcgtgt ttcgacccc actatagca aattacgca gtttttgat cccccagcg ctggatcct gtgtaaaaa tattttttt ctttcagtt aatcgtcgc tattttgtt cgtgattat tggaggcct caacattaa caagcagcg ggaccattt cgccgctta tgtcctaaa tgacgttat tctcatttt aattttttt gatttccgt tattaatat tgttatttc cgggttcgg gctcgaata ctcaaatca accacttag attaattta cgaacatac cgaagctcc gcctcgctc gtaattaga atatgtcgc caaatactt atttctgtt gttttacgt tccttgcct aacaccatt atttttctt taggtgaca gcagccttg ctctcaatc gtggtttca ataatgcaa acatacgag gatgaaaaa attaaaaac ctaagttgg atattacat catgtttct ttaggtttc gtgaccggg gcgttattt tgcgatata atcaaaatg aattttggg ttggatccc gccgttgct gttaacgtt actactctt aaaactgaa ctttgattt tttacttta tttttcttt atttttttt tattttttt tttattttt tttcgtgac actaaggtg ttccaagcg atcctgtgt gttcttctg gggactaca cttaatctc aacaaagaa ggggatcac aagtctgca gacagagcc aacaatgtt atactctgc attaatatt atcctaatg gctacaaat attctaatc agtgcattt gaaatgaag gaggatagc aataaaccg tgattagtg tttaagtga cagcataat gtatttatt tatggaata ggtttataa aatcatgtc aaaagggac tatcaagtt gagtaagat ttacattta ggattatgt gtaaaaaga acatcggga tgcattgta ggatactct cttagccta ttttttgtg tattattca agtgcattt acctagtga tatctttaa tagtcagat atatttata ttaattaaa taaaagtat ttccaaaga cacttctat atttagtac gtaatcaat taaataaat tgttataat gaga
- >TC031194 RC
taaaagtcg agataatgc aacgagtgg gagacagtt tgctcaaga ttagagtaa taacatttc gtattgctc ttgtggtta atagctttg acattattt gcaatttgc agagtccac cacattctc ttatccgcc ttggatgtt tctgtcttc tgtgccgtt ttgaacctt ttgtcacct cgccacgac ttcatgagt cagtcactc aatacgttt aatgtttgc attgaaagc caaaattgt tgcaattac gacgacggg tgcgtcact accattggt cgaggaaaa ttgatcgtg gtagagctt attcccgcg gcattgcct ggaaggttg ttttaatcg aaagtcgac ggattaacg ttataccaa gcattatgt tttatcaac gtttcactc actatctcc tctagacaa ttgtacaag tgcttttgt tttcgtttg atcgatata tcttgtcca ggtagaaaa atgtctatt tctccagat gcttatcgt ccccagtga ttcgtgacc actctgtaa caatggcaa gtgaaaata aagctggta gatcccaaa ctgatggga ataccatca gtgattata tcctgaaat ccttggacg caaataaat acgctacaa agaagacca tagcgcaag gactcctcg acgttgcct tactcactg ccaatgcct cccagctga agtacgtcc tccaagtcg gggaaaaac acgagttct acgtattga tgctaaccc taatcagca tatccatag ttctgcagg tgctcgtgg ggctccttt gtctcacac ttcatttaa tgagggact gtcggttgc acctcgcgg actaccact tttccgcca actttctta actatataa ccacaattt tcgcttttg ttataacag ttttgaatt tgcttattt cgtgtttcg accccacta tagcaaatt acgcagttt ttgatcccc cagcgctgg atcctgtgt aaaaatatt tttttcttt cagttaatc gtcgctatt ttgttcgtg attattgga ggcctcaac attaacaag cagcgggac catttcgcc gcttatgtc ctaaatgac gttattctc attttaatt tttttgatt tccgttatt aatattgtt atttccggg ttcgggctc gaatactca aatcaacca cttagatta atttacgaa cataccgaa gctccgcct cgctcgtaa ttagaatat gtcgccaaa tacttattt ctgttgttt tacgttcct tgcctaaca ccattattt ttctttagg tgacagcag ccttgctct caatcgtgg tttcaataa tgcaaacat acgaggatg aaaaaatta aaaacctaa gttggatat tacatcatg tttctttag gtttcgtga ccggggcgt tattttgcg atataatca aaatgaatt ttgggttgg atcccgccg ttgctgtta acgttacta ctcttaaaa ctgaacttt gatttttta ctttatttt tctttattt ttttttatt tttttttta ttttttttc gtgacacta aggtgttcc aagcgatcc tgtgtgttc ttctgggga ctacactta atctcaaca aagaagggg atcacaagt ctgcagaca gagccaaca atgttatac tctgcatta atattatcc taatggcta caaatattc taatcagtg catttgaaa tgaaggagg atagcaata aaccgtgat tagtgttta agtgacagc ataatgtat ttatttatg gaataggtt tataaaatc atgtcaaaa gggactatc aagttgagt aagatttac atttaggat tatgtgtaa aaagaacat cgggatgca ttgtaggat actctctta gcctatttt ttgtgtatt attcaagtg catttacct agtgatatc tttaatagt cagatatat ttatattaa ttaaataaa agtatttcc aaagacact tctatattt agtacgtaa tcaattaaa taaattgtt ataatgaga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
