Status
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Gene information
General information
TC010587 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 5364565..5368343
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010587
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07294

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010587 RA
      MAGMLTITS RNVPRTVLR TLNSYLEKC VTEVPPKHR 
      AYHQGLKAI RKAAVKPSH SVFARPQSI CCKQVRYFR 
      SSAALFEEK IVTVPPFAD SVSEGDVRW EKKKGDQVA 
      EDEVVLEIE TDKTSVPVP APANGIIEE MYVEDGATV 
      KAGQNLFKL KLTGDAPVK KAPAEKAAE PAAAPPPPS 
      PAAAATPPP PPKPAAPAA GGPPPPAPP RPAAPLSSI 
      PVAAIRHAQ AIEAATVKV PPQDPTKEI SGTRTEQRV 
      KMNRMRLKI AERLKQAQN VNAMLTTFN EIDMSYIME 
      FRKANQDAF QKKYGLKLG FMSAFVKAS AYALQDQPV 
      VNAVIDGQE IIYRDYVDI SVAVATPKG LVVPVVRNV 
      ERMSYADIE LAINALGEK ARKGSLAVE DMDGGTFTI 
      SNGGVFGSL LGTPIINPP QSAILGMHG IFERPVAVK 
      GQVVIRPMM YIALTYDHR LIDGREAVF FLRKIKQAV 
      EDPRVMLAG L
    CDS sequence
    • >TC010587 RA
      atggcgggt atgcttacg ataacgtct agaaatgta 
      ccacgaaca gttttaaga accttaaac agttattta 
      gaaaaatgt gtgacagag gtacctccc aagcacaga 
      gcgtaccat caggggtta aaggcgata agaaaagcg 
      gccgtgaag cctagtcac tcagtattt gcaaggccc 
      cagtctatt tgttgtaaa caagtgagg tatttccgg 
      tcttctgcc gcccttttt gaggagaaa atcgtaact 
      gtaccccca ttcgcagac tcagtctcc gaaggtgat 
      gtaaggtgg gaaaagaaa aaaggcgac caagtggct 
      gaagatgag gtcgttttg gagatcgaa acggacaaa 
      acctcggtt ccggtacct gccccagcc aatggtatt 
      atcgaggaa atgtatgtt gaagatggt gccactgtg 
      aaagctggc caaaacctc ttcaaattg aaacttact 
      ggggatgct ccggttaaa aaagcccca gctgagaag 
      gctgccgag ccagcggcc gctccaccg ccaccaagc 
      cccgctgcc gccgccacg ccgccgcca ccaccaaag 
      cccgcggct ccagcggca ggggggccg ccaccgccg 
      gcgcccccg cggccagca gcgcccctc agctcgata 
      ccagtggct gccataaga catgcccag gccattgaa 
      gctgccact gttaaagtg cccccacaa gaccccacc 
      aaagaaatt tcaggaaca cgaactgaa caaagagtt 
      aagatgaac aggatgcgt ttgaagatt gcagagagg 
      ttgaaacaa gcccagaat gttaatgcc atgttaacc 
      actttcaac gagattgat atgtcatac atcatggag 
      ttccgaaag gccaaccag gacgctttc cagaagaaa 
      tacggcctg aagctgggc ttcatgtcg gcatttgtc 
      aaggcctcg gcttacgcc ctccaagac cagcccgtt 
      gtgaacgcc gtcatcgat ggccaggaa atcatctac 
      agagactac gtcgacatc tccgtagcc gtggctaca 
      cccaagggt ctggtcgtt cctgtcgtc aggaatgtt 
      gaaagaatg agttacgcg gacatcgaa ttggccatc 
      aacgccctt ggcgaaaag gcgcggaaa gggagtttg 
      gccgtggaa gacatggat ggaggaact ttcacgatc 
      agtaacggt ggcgtgttc ggttcgctc ctgggaacc 
      cccattatc aacccaccg cagtcggct attcttgga 
      atgcacggg attttcgaa agacctgtg gccgttaaa 
      ggacaagtt gtgattcgc cccatgatg tacatagcc 
      ctaacgtac gatcacaga cttatcgac ggacgtgaa 
      gcagttttc ttcctccga aaaatcaaa caagcagtg 
      gaagatccg cgagttatg ttagccggt ctctaa
    mRNA sequence
    • >TC010587 RA
      tgcctcgaa atttgacat ttgagtgat ctttttaaa 
      tcaaaagat ctccaagtt gtggcagca cacgaaaag 
      gggctgctg tccgaaaat ttgcaaaaa tttcacatc 
      ataatttag tagaatggc gggtatgct tacgataac 
      gtctagaaa tgtaccacg aacagtttt aagaacctt 
      aaacagtta tttagaaaa atgtgtgac agaggtacc 
      tcccaagca cagagcgta ccatcaggg gttaaaggc 
      gataagaaa agcggccgt gaagcctag tcactcagt 
      atttgcaag gccccagtc tatttgttg taaacaagt 
      gaggtattt ccggtcttc tgccgccct ttttgagga 
      gaaaatcgt aactgtacc cccattcgc agactcagt 
      ctccgaagg tgatgtaag gtgggaaaa gaaaaaagg 
      cgaccaagt ggctgaaga tgaggtcgt tttggagat 
      cgaaacgga caaaacctc ggttccggt acctgcccc 
      agccaatgg tattatcga ggaaatgta tgttgaaga 
      tggtgccac tgtgaaagc tggccaaaa cctcttcaa 
      attgaaact tactgggga tgctccggt taaaaaagc 
      cccagctga gaaggctgc cgagccagc ggccgctcc 
      accgccacc aagccccgc tgccgccgc cacgccgcc 
      gccaccacc aaagcccgc ggctccagc ggcaggggg 
      gccgccacc gccggcgcc cccgcggcc agcagcgcc 
      cctcagctc gataccagt ggctgccat aagacatgc 
      ccaggccat tgaagctgc cactgttaa agtgccccc 
      acaagaccc caccaaaga aatttcagg aacacgaac 
      tgaacaaag agttaagat gaacaggat gcgtttgaa 
      gattgcaga gaggttgaa acaagccca gaatgttaa 
      tgccatgtt aaccacttt caacgagat tgatatgtc 
      atacatcat ggagttccg aaaggccaa ccaggacgc 
      tttccagaa gaaatacgg cctgaagct gggcttcat 
      gtcggcatt tgtcaaggc ctcggctta cgccctcca 
      agaccagcc cgttgtgaa cgccgtcat cgatggcca 
      ggaaatcat ctacagaga ctacgtcga catctccgt 
      agccgtggc tacacccaa gggtctggt cgttcctgt 
      cgtcaggaa tgttgaaag aatgagtta cgcggacat 
      cgaattggc catcaacgc ccttggcga aaaggcgcg 
      gaaagggag tttggccgt ggaagacat ggatggagg 
      aactttcac gatcagtaa cggtggcgt gttcggttc 
      gctcctggg aacccccat tatcaaccc accgcagtc 
      ggctattct tggaatgca cgggatttt cgaaagacc 
      tgtggccgt taaaggaca agttgtgat tcgccccat 
      gatgtacat agccctaac gtacgatca cagacttat 
      cgacggacg tgaagcagt tttcttcct ccgaaaaat 
      caaacaagc agtggaaga tccgcgagt tatgttagc 
      cggtctcta agagatatt aggaaagtg tacagtacc 
      taccgaaac caattctca caatcacgc aaatgtttt 
      ctctttgta aagtttgaa aataacgca ttttctgct 
      ttttagggt gatggtaac gttctcttt aactttaca 
      aatgggaag catgtaaaa gtgcacgct ttttcattc 
      tttgtcgaa tttcataac agttttttt ctgctatac 
      agattcatt gtaggtaat gaatttatt gtataagtt 
      ttattaaag tattgacgt atgtattgt ggtgccctt 
      tattttgtt atttctttg ttggtcatt aataaaaac 
      gttaaacaa attttgttc ctgttgaag aaaagtaca 
      attcggtat taacgatag cggaatgta ataatcgat 
      tatttataa aacatgtct aaatataca ttgtgagaa 
      atatactgt ggtggtctt tgttattta tttattgtt 
      caatataaa tttgaaaaa gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY