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Gene information
General information
TC009150 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3786080..3788235
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009150
    iBeetle screen iB_07292

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009150 RA
      MDTWVKLNG QTAGRDKTA RLLQYLSRI 
      LWHRLQHSN KDQVNAFKN LEFQLSTFR 
      KLLRFGKCL DVIYSTVAL FDHGDSTVR 
      HTIILSRIA NSLFLLADH LLWLGRADV 
      CNIDTQKWS RISNKYWLY SITMNLVRD 
      FYEISCIIK AQKRTIVPD CGVQNVNDL 
      YKILSRTLT VLQCHKGVM IDTVKNACD 
      FFIPLTALG HTKLSPGAV GWLGVVSSL 
      AGLLVLVDP KIKLSPA
    CDS sequence
    • >TC009150 RA
      atggatact tgggttaaa ttaaatggt cagacagct 
      gggagagac aaaaccgca agattgctg caatatttg 
      agccgaatc ctctggcac cgcttgcaa cacagtaac 
      aaggaccaa gtcaatgcg tttaaaaat ctggaattc 
      cagttgagc acatttcga aaattgctc agatttggc 
      aaatgcctt gatgttatt tactcaacg gtggctctc 
      ttcgaccac ggggactcc acagtgcgc cacacgata 
      atcctgagc cggattgcc aactcgttg tttctttta 
      gccgatcat ttgctgtgg ctaggccgt gcagatgtc 
      tgtaatatc gacacgcag aaatggagc cgcatttca 
      aacaaatac tggctgtat tccatcaca atgaactta 
      gttagggac ttttacgag atttcttgt attattaaa 
      gcgcagaaa cgaacaatt gtacctgat tgcggtgtt 
      cagaatgtt aacgacttg tataaaatt ctttctcgg 
      actttgaca gtgctacag tgtcataag ggggtcatg 
      attgacact gtcaaaaac gcgtgcgat tttttcatc 
      cccttgacg gcccttggg cacacgaag ctgtccccg 
      ggggccgtg gggtggctg ggggttgtc tcgtcactg 
      gctgggctt ctggtcctc gtggaccct aaaataaag 
      ctgtcccca gcctga
    mRNA sequence
    • >TC009150 RA
      gcgaaacaa aacaatgag ctgtcaaac tccgaagat 
      aaatatttg gtactttga ccggttaat taacgttag 
      cgacaggtt ttagcagtg tcacgatgg atacttggg 
      ttaaattaa atggtcaga cagctggga gagacaaaa 
      ccgcaagat tgctgcaat atttgagcc gaatcctct 
      ggcaccgct tgcaacaca gtaacaagg accaagtca 
      atgcgttta aaaatctgg aattccagt tgagcacat 
      ttcgaaaat tgctcagat ttggcaaat gccttgatg 
      ttatttact caacggtgg ctctcttcg accacgggg 
      actccacag tgcgccaca cgataatcc tgagccgga 
      ttgccaact cgttgtttc ttttagccg atcatttgc 
      tgtggctag gccgtgcag atgtctgta atatcgaca 
      cgcagaaat ggagccgca tttcaaaca aatactggc 
      tgtattcca tcacaatga acttagtta gggactttt 
      acgagattt cttgtatta ttaaagcgc agaaacgaa 
      caattgtac ctgattgcg gtgttcaga atgttaacg 
      acttgtata aaattcttt ctcggactt tgacagtgc 
      tacagtgtc ataaggggg tcatgattg acactgtca 
      aaaacgcgt gcgattttt tcatcccct tgacggccc 
      ttgggcaca cgaagctgt ccccggggg ccgtggggt 
      ggctggggg ttgtctcgt cactggctg ggcttctgg 
      tcctcgtgg accctaaaa taaagctgt ccccagcct 
      gattaattg acattattt atggtgata ggttttttg 
      taacatttg tttaatata aattaccaa ttacgtcat 
      taattattt taccaactg caccaacat ctgaaattg 
      aaatgaaat catgggtag ttttaagtg agctatggc 
      gaaattcgt cgattgttt tatgttgat gggcactgc 
      cctttcgct tcgcccaga aatgggacg ccctctcct 
      gcaagatgt tggcacaac tcgggtgaa cctctcctt 
      aatgatcca ggaatttaa aatttttga aagtaaata 
      cttggtgag tttttttga tttttttct aaatgaaaa 
      ataggagat cgagttagg gggcttgct gacccagcg 
      atggatcga tccacgtct tggggcgaa tatcaggac 
      ccggaaaat ggcaataaa tgggcaaat ttggaaacc 
      taaaatcgt aaatgtaat tggagaaag gagaaaaag 
      tgttgaaga cagcgcttt ttggagggg ttttgagga 
      aaatgttgt cctgagcga aaaaatcag cgttaggag 
      tttgtgtac ttgggatta tcacaaatt tccgtttga 
      tacacaaat gtgtgacgt taaatttgg atcgtgtaa 
      caaaaatga attattttt gtatttgtt ttatttttt 
      tcatattta gttaggttt cctgctgac gaagttgag 
      ttaatttgg gatttgacg gcggtacaa gtgaccgca 
      aatttggat ttcaatgac tttgagatt aaaaatatc 
      gaaacaagt gttgaaatc gatataaga agtgaaggt 
      aaacattgt taattaaat tattatcac aaaaacagt 
      tttttaggt aatttcagt gttgcagtt acacaattt 
      gttctaaaa cgccaatca aaacatcac attttttgc 
      gtttttttg cctctatgt cttctcagt ttactcaat 
      tacttatcg ttttggatg agtaagcag tttcgacgt 
      tttttcaat gttatagtt ttaatttgc gtattaatt 
      tcgtatcgt atgtcatgt tttacgcac ccgacgcca 
      tatttgaaa gcaagcgat atttttatt ttaatttgt 
      ggttgtttt tccctaata attcttacc gaaacgttt 
      tttatggat attattacg actttggac gacttccac 
      tatcctaag aagaactca aagtgggtc ttaaagctc 
      tcataccca attttttac atctttgta atggcactg 
      tacgcattt tggtatttg tgattgtaa taaaagttt 
      cttattgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY