| General information | |||
| TC014624 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 12547507..12556661 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014624 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07288 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014624 RA
MSNSVVKVT DSDSTIDDN LLLLIGEDG TVTPSKDVV KAYLSGKSG ATNLQVMHI NKSDNEGID VAVDDMLYV PDAEADDLP DNKETSFDD IAYNLYNIK LDHDYTPHC GYSEDDELA RTLSILDGD EDNDSPSVT KPLESRSLR SNAHESPKP KTKVATPLK TITKDQAKK IKTRTKSET SIKDGESPS DAKKVLRPE HKPKDNVVK SLKTIKPVE PESPKAEKP QPVDPKSAE KKQPKKDRK VAKPVPDDF ALFSTPDII RRVGGKEPI SPELSSPDQ NKPAKIETR SKSVSDSAQ VSPKQNRLS LDGKVVDKS PSKKPTSDN KAKKIMFDN TKTVAELEP KPCESNTNV EPAIPEPMI ANNEDKSFP ALPLETPDL SGGENNMAL DGNGLDLDP SLLENINND LISEDILYQ VAQSLVSNT ELQNAIDKS LNEENLGLD SSVQEVQQD DVVHNNQTE QVTSSVPQK GTQIVRPDG RIVVIPPIE RPTTRSRNK NKDADKPTP KFVHKPLDE EHVSGNELD SSEEEEEES EDDPNKLWC ICNQPHNNR FMICCDTCE EWYHGKCVN ITKAMGQQM EAEGREWIC LFCKDPLLK RPQAAARRI RKASRNSRQ STDSSGSTK VPETRPSSV PCVVCQKPS RTNSIFCSE SCILAHAQG VERVVVFER STGNMLTGN KAPSAANLE QWLKEHPGY EVVRSAGKI VTTKQTQKS PPSKIKILK GSETPAMHF QNRMKNLNK TVPKSPQVV KVGNTSGLK VLNRQISTA QLREKVSNS LKEAASPTK FKIVTTSTK SSSSPANRE KPTTPKVQK VRNASTPQA KQENIRDNV KKTVYEQLT NRLKMVDDL KLSEEELKN ISLEIESQL YKCFGDTGQ KYRNKYRSL IFNIKDIKN QTLWRRICE KTINPYQLV RLSPDDLAS QELALWRER ETKHQLDMI KKSELELLQ CNRQYVLKT HKGEQILED NRPTDNLDN TEVITSLTD NATGEKTDR KDDRKKHSH RDKERKSTK DKSEKSHHS HRSTSRDHD RSRKRSRSR DRSSKHVEK RRSRSRDRH RRSSHKSSR HKKESHRGS STEKLDKKS KEILEQLVD NKIVPPLED RLWKHVPQE DVLPNSVAE SDSDHEPTS TVTIPTPPR NNNDSDEFA AGHGLSENK NNDKYPVKE QTEVDHSMS PVVKPKVEE IWRGTINMV DVAQISITA HEVSGDCSG LATELPANL DIVGRISPD TVWDYIGKM KCSNSKIIS VVRLNAINV EEKMPYLAL YSYLSSRNR LGVVKSVNK AVKDFYILP LASQKPIPQ ALLPLNGPG FEEARPALL LGILVRDKR KRPYVDSAS SAPSVKKTK TEMPSRSYT PPPVKDSRL KLPLPPPIG TDQDGDEPY SPEDSDPDS IIPATTRSV SNSSTIFLD ADAAATQAF QPFVNKFEE IPGLDNATL PSSTQEIQR QMEKLNKEI EMQKSEINS ISQNIATAS NEIGTSALA NIALPSNLQ QILDSIKTI GSTSALESL ETKPQAVDA LQESSDFTI PLMLPTTFS RHSASSENT IPLNLPSKK KVASDAEKT SVLSSLSEE DLIRKAEEM LEASETTDK LAIPPSVSS SSQILPKVE MKQPPIPGL EDDV
- >TC014624 RA
atgtcaaac agcgtcgtt aaggtaaca gattccgat tcaacaatt gatgacaat ttacttctt cttattggt gaagatgga actgtcaca ccgagtaaa gacgtcgtc aaagcttat ttaagtggc aaatcgggc gccaccaac ctccaagta atgcatatt aataaatca gataatgag ggcattgat gttgcggtg gatgacatg ttatatgta ccggacgct gaagctgac gaccttcca gataataaa gagacgtct tttgacgac atagcttac aatttatac aacataaag cttgatcat gattacaca cctcattgt gggtattct gaggatgat gagttggcg cgaactctt agtatatta gacggggac gaagacaat gactctcct tccgtcaca aaaccacta gaaagtcga tcccttaga agcaacgca catgaaagc cccaaaccc aaaacaaag gtagcaaca ccgctaaaa acaatcacc aaagatcaa gcaaaaaag attaaaaca aggacaaag agtgagaca tcgatcaaa gacggggaa agtccgtca gatgctaaa aaagtcctc agaccggag cataaaccc aaggacaat gttgtcaag tctttgaaa acaataaag ccagtcgag cctgaatca cctaaagcg gaaaagccg caaccagtt gatcccaag tcagctgaa aagaaacag cccaagaaa gatcgcaaa gtggcgaag ccagtccca gacgatttc gccctgttt tcaacccct gatatcatt agacgagtg ggagggaag gaaccaatt tcgcccgaa cttagcagt ccggatcaa aacaaaccg gcgaaaatc gaaactcga tcgaagtca gtctcagac tccgctcaa gtttcgccc aaacagaat cgcttgagt ctcgacggc aaagtagtc gataaatcc cccagtaaa aaaccgact agcgataat aaagcgaag aaaatcatg tttgataac accaaaact gtggcggaa ttggaacca aaaccgtgc gaaagtaac acgaacgtg gaacctgca atccccgag ccaatgatt gcgaataat gaagataag tcgtttccg gctttacct ctcgaaact cctgattta agtggaggt gagaataac atggctttg gacgggaat gggctcgat cttgaccca agcttgttg gaaaacatc aataacgac ctaatatct gaagatatt ctgtaccaa gttgcgcag tcgttggtg tcaaacacg gagctacag aacgcgata gataagagc ttgaatgag gagaatttg ggcctcgat tcgagcgtc caagaggtg cagcaagac gacgtggtt cacaataat caaacagag caggtaacg tcatctgtg ccacaaaaa ggaactcaa atcgtccgc cctgacggt aggatagtc gtaatccct ccaatagaa cgccccaca accaggagt cgaaacaag aacaaagat gccgataaa ccaacccca aaatttgtc cacaaaccc ttggatgaa gagcacgtc tcaggcaac gaactcgac agttctgag gaagaagag gaagaatcg gaggatgat ccgaacaaa ctttggtgt atttgtaac caacctcac aacaaccgg ttcatgatc tgttgcgat acgtgcgaa gaatggtac cacggaaaa tgcgtgaat atcacaaaa gcgatggga cagcaaatg gaagcggaa ggaagagaa tggatttgt ctattttgt aaagatcca cttttgaag cgaccacaa gccgcggcc agaagaata agaaaagct tcgcggaat tcgcgccag tctacggac agctctggc agtaccaaa gtaccagag acacggcca agttcagtc ccgtgtgtc gtttgccag aaaccgtca agaactaat tcgattttc tgcagcgaa agctgcatt ttggcgcac gcacaaggc gtcgaacga gtggtcgtg ttcgagaga tcaactgga aacatgttg accgggaac aaggccccc agtgctgcc aacttggag caatggctc aaagaacac ccaggttac gaagtcgtt cgttccgct ggtaaaata gtaacgaca aagcaaacg caaaaaagt ccgccatcg aaaataaaa attttaaaa ggttccgaa acacctgct atgcatttc caaaatcga atgaaaaat ttgaataaa accgtaccg aaaagtcca caagtggtc aaagtcggt aatacatca ggtttgaaa gttcttaat cgacaaatt tcgacggct cagctgagg gaaaaggtc agtaattca cttaaagag gccgcatca cctaccaag tttaaaatc gttacaact tcgaccaaa tcgagtagt tcgccggcg aatagagaa aaacccaca acacctaaa gtacaaaaa gtgcgaaat gcaagtaca cctcaagcc aaacaggag aatatcaga gataatgtc aagaaaaca gtttacgag cagttgacc aaccgatta aaaatggtg gatgatttg aagttgtct gaggaggaa ttgaaaaac atttcgctc gaaattgaa tctcaactg tacaaatgc ttcggcgat acggggcag aaatacagg aacaaatac agaagttta atttttaat attaaagac ataaagaat caaacactg tggaggcgg atttgtgag aagaccatt aatccgtac caactcgtc aggttatcg cccgacgac ttagccagc caggagttg gccctttgg cgggagcgc gaaaccaaa caccagtta gacatgatt aaaaaatcc gagctcgaa ttactccaa tgcaacaga caatacgtg ttgaagaca cacaaaggt gaacaaatt ttggaagac aatcgtcca acggacaac ctcgacaac accgaggtc ataacaagt ctgacagat aacgcaacg ggggagaaa accgatcgt aaagacgac agaaaaaaa cacagtcat agggataaa gagcggaaa tcgacgaaa gataagtcc gaaaaaagt catcacagt cacagatcg acgagtcga gatcacgat cgcagtcgg aaacgttcg cgcagcagg gataggagt tcaaaacat gtggagaaa cggagatcc agaagcagg gatcgacat aggaggtcg tcacataaa agttcaagg cataaaaag gagtcgcat cgagggtcg tccactgag aaactcgat aaaaaatcg aaggagatt ttagaacag ttggttgat aataaaatt gtgcctccg ctggaagac aggctgtgg aagcatgtg ccacaagag gacgtttta ccaaattcg gttgctgaa agtgacagt gaccacgaa ccgacttcc actgtcact attccaact ccaccacga aataacaac gattcggat gaatttgcg gctggccac ggtttatcg gaaaataaa aataatgat aaatatcct gtaaaggag cagactgaa gtggaccac tcgatgtca ccagtggtt aagccaaaa gttgaggag atttggcgt ggtaccatt aatatggta gatgtggca caaatctcg atcacagct cacgaagtg tcaggcgat tgtagtggc ttggccact gaactaccc gcaaatttg gacattgtt ggtagaatt tcccccgat accgtgtgg gactatatc ggaaaaatg aaatgttcc aacagtaaa atcatatca gtggtgcgc ttaaacgca atcaatgtg gaagaaaaa atgccgtat ttggctctc tacagttac ttatcgagc aggaatcga ctcggtgtt gttaaaagt gtgaacaag gccgtgaaa gatttttac attttacca ctggcctct cagaaaccg atcccacag gctttgtta cctctcaac ggtccaggt ttcgaggag gcacggcct gctttgctc ctgggaatc cttgttcga gacaagaga aaaaggcca tatgttgat tcagcgtcc agtgccccc agtgttaaa aaaacgaaa acagagatg ccttctagg tcgtacaca ccgccacct gtcaaagat tcccggctt aaattacca cttccacca ccgataggc acggaccag gatggagat gagccgtac agtcctgag gactcggac cccgacagc ataattccc gcaaccact cgaagtgtt tccaacagt tcgacaatc tttctcgac gctgacgcc gcggcaaca caagccttt caaccgttc gttaacaag tttgaagag attccagga ctggataac gcaactttg ccgtcaagt acgcaagaa atccaacgc caaatggag aaactcaac aaagaaatc gaaatgcaa aagtcggaa atcaacagt atatcgcaa aacatcgcc actgcatcg aacgaaatc gggacttcg gctctggcg aacatagcg ctcccgagc aatttgcag caaattctg gacagcatt aaaacgatc ggttccact tcggcttta gaatcgttg gagacgaag ccgcaagct gtggacgcg ctgcaggaa agttccgac tttacgatt cctttgatg ttgcctacg acgttttcg cgccattcc gccagttcc gaaaatacc attcctttg aatttgcca agtaagaag aaagtggca agcgacgct gagaaaacg agcgttttg agttcgctg tctgaggag gatttgatt cgaaaggcg gaagagatg cttgaagcg tccgaaaca acagacaag ctagcaatt ccaccgagt gtttcaagt tcgtcacaa atactgccc aaggttgag atgaaacaa ccgccaata cctggactg gaggatgat gtctaa
- >TC014624 RA
gttcttctt ccactcttg ttgttccat aaaatggcg gtattgagc gagttgtgt tatttttag cgctttaaa cacctcaaa ttcgagtaa ttggtacat gtgaacaac ataatccac gattataaa tccgctgct ttgtatata tcgtataaa tggagagtc cggtggact gttgaagct tttccagca actacaaac aaaatatta aagtgagtt cagtgccct gtgcaagaa cactaatgt ttggattgg aaacttgag ccgtttgcg tgagatatg caccaagtg ttaataatg tattaatgt gtgttttca tcgtcacac gaacatcca aaggacagt gatttaaga cacttgctt cgatgatga taaggttga agtcttgct gaaaacggc tggtgtgac atcaagata caaaaggga tattgttgg tcacgatgt caaacagcg tcgttaagg taacagatt ccgattcaa caattgatg acaatttac ttcttctta ttggtgaag atggaactg tcacaccga gtaaagacg tcgtcaaag cttatttaa gtggcaaat cgggcgcca ccaacctcc aagtaatgc atattaata aatcagata atgagggca ttgatgttg cggtggatg acatgttat atgtaccgg acgctgaag ctgacgacc ttccagata ataaagaga cgtcttttg acgacatag cttacaatt tatacaaca taaagcttg atcatgatt acacacctc attgtgggt attctgagg atgatgagt tggcgcgaa ctcttagta tattagacg gggacgaag acaatgact ctccttccg tcacaaaac cactagaaa gtcgatccc ttagaagca acgcacatg aaagcccca aacccaaaa caaaggtag caacaccgc taaaaacaa tcaccaaag atcaagcaa aaaagatta aaacaagga caaagagtg agacatcga tcaaagacg gggaaagtc cgtcagatg ctaaaaaag tcctcagac cggagcata aacccaagg acaatgttg tcaagtctt tgaaaacaa taaagccag tcgagcctg aatcaccta aagcggaaa agccgcaac cagttgatc ccaagtcag ctgaaaaga aacagccca agaaagatc gcaaagtgg cgaagccag tcccagacg atttcgccc tgttttcaa cccctgata tcattagac gagtgggag ggaaggaac caatttcgc ccgaactta gcagtccgg atcaaaaca aaccggcga aaatcgaaa ctcgatcga agtcagtct cagactccg ctcaagttt cgcccaaac agaatcgct tgagtctcg acggcaaag tagtcgata aatccccca gtaaaaaac cgactagcg ataataaag cgaagaaaa tcatgtttg ataacacca aaactgtgg cggaattgg aaccaaaac cgtgcgaaa gtaacacga acgtggaac ctgcaatcc ccgagccaa tgattgcga ataatgaag ataagtcgt ttccggctt tacctctcg aaactcctg atttaagtg gaggtgaga ataacatgg ctttggacg ggaatgggc tcgatcttg acccaagct tgttggaaa acatcaata acgacctaa tatctgaag atattctgt accaagttg cgcagtcgt tggtgtcaa acacggagc tacagaacg cgatagata agagcttga atgaggaga atttgggcc tcgattcga gcgtccaag aggtgcagc aagacgacg tggttcaca ataatcaaa cagagcagg taacgtcat ctgtgccac aaaaaggaa ctcaaatcg tccgccctg acggtagga tagtcgtaa tccctccaa tagaacgcc ccacaacca ggagtcgaa acaagaaca aagatgccg ataaaccaa ccccaaaat ttgtccaca aacccttgg atgaagagc acgtctcag gcaacgaac tcgacagtt ctgaggaag aagaggaag aatcggagg atgatccga acaaacttt ggtgtattt gtaaccaac ctcacaaca accggttca tgatctgtt gcgatacgt gcgaagaat ggtaccacg gaaaatgcg tgaatatca caaaagcga tgggacagc aaatggaag cggaaggaa gagaatgga tttgtctat tttgtaaag atccacttt tgaagcgac cacaagccg cggccagaa gaataagaa aagcttcgc ggaattcgc gccagtcta cggacagct ctggcagta ccaaagtac cagagacac ggccaagtt cagtcccgt gtgtcgttt gccagaaac cgtcaagaa ctaattcga ttttctgca gcgaaagct gcattttgg cgcacgcac aaggcgtcg aacgagtgg tcgtgttcg agagatcaa ctggaaaca tgttgaccg ggaacaagg cccccagtg ctgccaact tggagcaat ggctcaaag aacacccag gttacgaag tcgttcgtt ccgctggta aaatagtaa cgacaaagc aaacgcaaa aaagtccgc catcgaaaa taaaaattt taaaaggtt ccgaaacac ctgctatgc atttccaaa atcgaatga aaaatttga ataaaaccg taccgaaaa gtccacaag tggtcaaag tcggtaata catcaggtt tgaaagttc ttaatcgac aaatttcga cggctcagc tgagggaaa aggtcagta attcactta aagaggccg catcaccta ccaagttta aaatcgtta caacttcga ccaaatcga gtagttcgc cggcgaata gagaaaaac ccacaacac ctaaagtac aaaaagtgc gaaatgcaa gtacacctc aagccaaac aggagaata tcagagata atgtcaaga aaacagttt acgagcagt tgaccaacc gattaaaaa tggtggatg atttgaagt tgtctgagg aggaattga aaaacattt cgctcgaaa ttgaatctc aactgtaca aatgcttcg gcgatacgg ggcagaaat acaggaaca aatacagaa gtttaattt ttaatatta aagacataa agaatcaaa cactgtgga ggcggattt gtgagaaga ccattaatc cgtaccaac tcgtcaggt tatcgcccg acgacttag ccagccagg agttggccc tttggcggg agcgcgaaa ccaaacacc agttagaca tgattaaaa aatccgagc tcgaattac tccaatgca acagacaat acgtgttga agacacaca aaggtgaac aaattttgg aagacaatc gtccaacgg acaacctcg acaacaccg aggtcataa caagtctga cagataacg caacggggg agaaaaccg atcgtaaag acgacagaa aaaaacaca gtcataggg ataaagagc ggaaatcga cgaaagata agtccgaaa aaagtcatc acagtcaca gatcgacga gtcgagatc acgatcgca gtcggaaac gttcgcgca gcagggata ggagttcaa aacatgtgg agaaacgga gatccagaa gcagggatc gacatagga ggtcgtcac ataaaagtt caaggcata aaaaggagt cgcatcgag ggtcgtcca ctgagaaac tcgataaaa aatcgaagg agattttag aacagttgg ttgataata aaattgtgc ctccgctgg aagacaggc tgtggaagc atgtgccac aagaggacg ttttaccaa attcggttg ctgaaagtg acagtgacc acgaaccga cttccactg tcactattc caactccac cacgaaata acaacgatt cggatgaat ttgcggctg gccacggtt tatcggaaa ataaaaata atgataaat atcctgtaa aggagcaga ctgaagtgg accactcga tgtcaccag tggttaagc caaaagttg aggagattt ggcgtggta ccattaata tggtagatg tggcacaaa tctcgatca cagctcacg aagtgtcag gcgattgta gtggcttgg ccactgaac tacccgcaa atttggaca ttgttggta gaatttccc ccgataccg tgtgggact atatcggaa aaatgaaat gttccaaca gtaaaatca tatcagtgg tgcgcttaa acgcaatca atgtggaag aaaaaatgc cgtatttgg ctctctaca gttacttat cgagcagga atcgactcg gtgttgtta aaagtgtga acaaggccg tgaaagatt tttacattt taccactgg cctctcaga aaccgatcc cacaggctt tgttacctc tcaacggtc caggtttcg aggaggcac ggcctgctt tgctcctgg gaatccttg ttcgagaca agagaaaaa ggccatatg ttgattcag cgtccagtg cccccagtg ttaaaaaaa cgaaaacag agatgcctt ctaggtcgt acacaccgc cacctgtca aagattccc ggcttaaat taccacttc caccaccga taggcacgg accaggatg gagatgagc cgtacagtc ctgaggact cggaccccg acagcataa ttcccgcaa ccactcgaa gtgtttcca acagttcga caatctttc tcgacgctg acgccgcgg caacacaag cctttcaac cgttcgtta acaagtttg aagagattc caggactgg ataacgcaa ctttgccgt caagtacgc aagaaatcc aacgccaaa tggagaaac tcaacaaag aaatcgaaa tgcaaaagt cggaaatca acagtatat cgcaaaaca tcgccactg catcgaacg aaatcggga cttcggctc tggcgaaca tagcgctcc cgagcaatt tgcagcaaa ttctggaca gcattaaaa cgatcggtt ccacttcgg ctttagaat cgttggaga cgaagccgc aagctgtgg acgcgctgc aggaaagtt ccgacttta cgattcctt tgatgttgc ctacgacgt tttcgcgcc attccgcca gttccgaaa ataccattc ctttgaatt tgccaagta agaagaaag tggcaagcg acgctgaga aaacgagcg ttttgagtt cgctgtctg aggaggatt tgattcgaa aggcggaag agatgcttg aagcgtccg aaacaacag acaagctag caattccac cgagtgttt caagttcgt cacaaatac tgcccaagg ttgagatga aacaaccgc caatacctg gactggagg atgatgtct aattttggc tggctgaag gaggtgttt cacaatgtt ttcgtcatt tttataaga ataggactt tatttgagt aaaattgtt ggaactttt atgtataga ctttagttg aaaactatg gattcttat taattcact tacatgttt ttaatcagt tttttatat tgtaaatat acaattgtt ttgtttcta tgagacatg taaactagg aaaatacta cagataagt gcaagcgat tattccatg taaattttg cgacctatg ttaaattct gtatagtta cttttattt agaaaactt gaaataaac catttcttt ttga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
