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Gene information
General information
TC014624 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12547507..12556661
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014624
    iBeetle screen iB_07288

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014624 RA
      MSNSVVKVT DSDSTIDDN LLLLIGEDG TVTPSKDVV 
      KAYLSGKSG ATNLQVMHI NKSDNEGID VAVDDMLYV 
      PDAEADDLP DNKETSFDD IAYNLYNIK LDHDYTPHC 
      GYSEDDELA RTLSILDGD EDNDSPSVT KPLESRSLR 
      SNAHESPKP KTKVATPLK TITKDQAKK IKTRTKSET 
      SIKDGESPS DAKKVLRPE HKPKDNVVK SLKTIKPVE 
      PESPKAEKP QPVDPKSAE KKQPKKDRK VAKPVPDDF 
      ALFSTPDII RRVGGKEPI SPELSSPDQ NKPAKIETR 
      SKSVSDSAQ VSPKQNRLS LDGKVVDKS PSKKPTSDN 
      KAKKIMFDN TKTVAELEP KPCESNTNV EPAIPEPMI 
      ANNEDKSFP ALPLETPDL SGGENNMAL DGNGLDLDP 
      SLLENINND LISEDILYQ VAQSLVSNT ELQNAIDKS 
      LNEENLGLD SSVQEVQQD DVVHNNQTE QVTSSVPQK 
      GTQIVRPDG RIVVIPPIE RPTTRSRNK NKDADKPTP 
      KFVHKPLDE EHVSGNELD SSEEEEEES EDDPNKLWC 
      ICNQPHNNR FMICCDTCE EWYHGKCVN ITKAMGQQM 
      EAEGREWIC LFCKDPLLK RPQAAARRI RKASRNSRQ 
      STDSSGSTK VPETRPSSV PCVVCQKPS RTNSIFCSE 
      SCILAHAQG VERVVVFER STGNMLTGN KAPSAANLE 
      QWLKEHPGY EVVRSAGKI VTTKQTQKS PPSKIKILK 
      GSETPAMHF QNRMKNLNK TVPKSPQVV KVGNTSGLK 
      VLNRQISTA QLREKVSNS LKEAASPTK FKIVTTSTK 
      SSSSPANRE KPTTPKVQK VRNASTPQA KQENIRDNV 
      KKTVYEQLT NRLKMVDDL KLSEEELKN ISLEIESQL 
      YKCFGDTGQ KYRNKYRSL IFNIKDIKN QTLWRRICE 
      KTINPYQLV RLSPDDLAS QELALWRER ETKHQLDMI 
      KKSELELLQ CNRQYVLKT HKGEQILED NRPTDNLDN 
      TEVITSLTD NATGEKTDR KDDRKKHSH RDKERKSTK 
      DKSEKSHHS HRSTSRDHD RSRKRSRSR DRSSKHVEK 
      RRSRSRDRH RRSSHKSSR HKKESHRGS STEKLDKKS 
      KEILEQLVD NKIVPPLED RLWKHVPQE DVLPNSVAE 
      SDSDHEPTS TVTIPTPPR NNNDSDEFA AGHGLSENK 
      NNDKYPVKE QTEVDHSMS PVVKPKVEE IWRGTINMV 
      DVAQISITA HEVSGDCSG LATELPANL DIVGRISPD 
      TVWDYIGKM KCSNSKIIS VVRLNAINV EEKMPYLAL 
      YSYLSSRNR LGVVKSVNK AVKDFYILP LASQKPIPQ 
      ALLPLNGPG FEEARPALL LGILVRDKR KRPYVDSAS 
      SAPSVKKTK TEMPSRSYT PPPVKDSRL KLPLPPPIG 
      TDQDGDEPY SPEDSDPDS IIPATTRSV SNSSTIFLD 
      ADAAATQAF QPFVNKFEE IPGLDNATL PSSTQEIQR 
      QMEKLNKEI EMQKSEINS ISQNIATAS NEIGTSALA 
      NIALPSNLQ QILDSIKTI GSTSALESL ETKPQAVDA 
      LQESSDFTI PLMLPTTFS RHSASSENT IPLNLPSKK 
      KVASDAEKT SVLSSLSEE DLIRKAEEM LEASETTDK 
      LAIPPSVSS SSQILPKVE MKQPPIPGL EDDV
    CDS sequence
    • >TC014624 RA
      atgtcaaac agcgtcgtt aaggtaaca gattccgat 
      tcaacaatt gatgacaat ttacttctt cttattggt 
      gaagatgga actgtcaca ccgagtaaa gacgtcgtc 
      aaagcttat ttaagtggc aaatcgggc gccaccaac 
      ctccaagta atgcatatt aataaatca gataatgag 
      ggcattgat gttgcggtg gatgacatg ttatatgta 
      ccggacgct gaagctgac gaccttcca gataataaa 
      gagacgtct tttgacgac atagcttac aatttatac 
      aacataaag cttgatcat gattacaca cctcattgt 
      gggtattct gaggatgat gagttggcg cgaactctt 
      agtatatta gacggggac gaagacaat gactctcct 
      tccgtcaca aaaccacta gaaagtcga tcccttaga 
      agcaacgca catgaaagc cccaaaccc aaaacaaag 
      gtagcaaca ccgctaaaa acaatcacc aaagatcaa 
      gcaaaaaag attaaaaca aggacaaag agtgagaca 
      tcgatcaaa gacggggaa agtccgtca gatgctaaa 
      aaagtcctc agaccggag cataaaccc aaggacaat 
      gttgtcaag tctttgaaa acaataaag ccagtcgag 
      cctgaatca cctaaagcg gaaaagccg caaccagtt 
      gatcccaag tcagctgaa aagaaacag cccaagaaa 
      gatcgcaaa gtggcgaag ccagtccca gacgatttc 
      gccctgttt tcaacccct gatatcatt agacgagtg 
      ggagggaag gaaccaatt tcgcccgaa cttagcagt 
      ccggatcaa aacaaaccg gcgaaaatc gaaactcga 
      tcgaagtca gtctcagac tccgctcaa gtttcgccc 
      aaacagaat cgcttgagt ctcgacggc aaagtagtc 
      gataaatcc cccagtaaa aaaccgact agcgataat 
      aaagcgaag aaaatcatg tttgataac accaaaact 
      gtggcggaa ttggaacca aaaccgtgc gaaagtaac 
      acgaacgtg gaacctgca atccccgag ccaatgatt 
      gcgaataat gaagataag tcgtttccg gctttacct 
      ctcgaaact cctgattta agtggaggt gagaataac 
      atggctttg gacgggaat gggctcgat cttgaccca 
      agcttgttg gaaaacatc aataacgac ctaatatct 
      gaagatatt ctgtaccaa gttgcgcag tcgttggtg 
      tcaaacacg gagctacag aacgcgata gataagagc 
      ttgaatgag gagaatttg ggcctcgat tcgagcgtc 
      caagaggtg cagcaagac gacgtggtt cacaataat 
      caaacagag caggtaacg tcatctgtg ccacaaaaa 
      ggaactcaa atcgtccgc cctgacggt aggatagtc 
      gtaatccct ccaatagaa cgccccaca accaggagt 
      cgaaacaag aacaaagat gccgataaa ccaacccca 
      aaatttgtc cacaaaccc ttggatgaa gagcacgtc 
      tcaggcaac gaactcgac agttctgag gaagaagag 
      gaagaatcg gaggatgat ccgaacaaa ctttggtgt 
      atttgtaac caacctcac aacaaccgg ttcatgatc 
      tgttgcgat acgtgcgaa gaatggtac cacggaaaa 
      tgcgtgaat atcacaaaa gcgatggga cagcaaatg 
      gaagcggaa ggaagagaa tggatttgt ctattttgt 
      aaagatcca cttttgaag cgaccacaa gccgcggcc 
      agaagaata agaaaagct tcgcggaat tcgcgccag 
      tctacggac agctctggc agtaccaaa gtaccagag 
      acacggcca agttcagtc ccgtgtgtc gtttgccag 
      aaaccgtca agaactaat tcgattttc tgcagcgaa 
      agctgcatt ttggcgcac gcacaaggc gtcgaacga 
      gtggtcgtg ttcgagaga tcaactgga aacatgttg 
      accgggaac aaggccccc agtgctgcc aacttggag 
      caatggctc aaagaacac ccaggttac gaagtcgtt 
      cgttccgct ggtaaaata gtaacgaca aagcaaacg 
      caaaaaagt ccgccatcg aaaataaaa attttaaaa 
      ggttccgaa acacctgct atgcatttc caaaatcga 
      atgaaaaat ttgaataaa accgtaccg aaaagtcca 
      caagtggtc aaagtcggt aatacatca ggtttgaaa 
      gttcttaat cgacaaatt tcgacggct cagctgagg 
      gaaaaggtc agtaattca cttaaagag gccgcatca 
      cctaccaag tttaaaatc gttacaact tcgaccaaa 
      tcgagtagt tcgccggcg aatagagaa aaacccaca 
      acacctaaa gtacaaaaa gtgcgaaat gcaagtaca 
      cctcaagcc aaacaggag aatatcaga gataatgtc 
      aagaaaaca gtttacgag cagttgacc aaccgatta 
      aaaatggtg gatgatttg aagttgtct gaggaggaa 
      ttgaaaaac atttcgctc gaaattgaa tctcaactg 
      tacaaatgc ttcggcgat acggggcag aaatacagg 
      aacaaatac agaagttta atttttaat attaaagac 
      ataaagaat caaacactg tggaggcgg atttgtgag 
      aagaccatt aatccgtac caactcgtc aggttatcg 
      cccgacgac ttagccagc caggagttg gccctttgg 
      cgggagcgc gaaaccaaa caccagtta gacatgatt 
      aaaaaatcc gagctcgaa ttactccaa tgcaacaga 
      caatacgtg ttgaagaca cacaaaggt gaacaaatt 
      ttggaagac aatcgtcca acggacaac ctcgacaac 
      accgaggtc ataacaagt ctgacagat aacgcaacg 
      ggggagaaa accgatcgt aaagacgac agaaaaaaa 
      cacagtcat agggataaa gagcggaaa tcgacgaaa 
      gataagtcc gaaaaaagt catcacagt cacagatcg 
      acgagtcga gatcacgat cgcagtcgg aaacgttcg 
      cgcagcagg gataggagt tcaaaacat gtggagaaa 
      cggagatcc agaagcagg gatcgacat aggaggtcg 
      tcacataaa agttcaagg cataaaaag gagtcgcat 
      cgagggtcg tccactgag aaactcgat aaaaaatcg 
      aaggagatt ttagaacag ttggttgat aataaaatt 
      gtgcctccg ctggaagac aggctgtgg aagcatgtg 
      ccacaagag gacgtttta ccaaattcg gttgctgaa 
      agtgacagt gaccacgaa ccgacttcc actgtcact 
      attccaact ccaccacga aataacaac gattcggat 
      gaatttgcg gctggccac ggtttatcg gaaaataaa 
      aataatgat aaatatcct gtaaaggag cagactgaa 
      gtggaccac tcgatgtca ccagtggtt aagccaaaa 
      gttgaggag atttggcgt ggtaccatt aatatggta 
      gatgtggca caaatctcg atcacagct cacgaagtg 
      tcaggcgat tgtagtggc ttggccact gaactaccc 
      gcaaatttg gacattgtt ggtagaatt tcccccgat 
      accgtgtgg gactatatc ggaaaaatg aaatgttcc 
      aacagtaaa atcatatca gtggtgcgc ttaaacgca 
      atcaatgtg gaagaaaaa atgccgtat ttggctctc 
      tacagttac ttatcgagc aggaatcga ctcggtgtt 
      gttaaaagt gtgaacaag gccgtgaaa gatttttac 
      attttacca ctggcctct cagaaaccg atcccacag 
      gctttgtta cctctcaac ggtccaggt ttcgaggag 
      gcacggcct gctttgctc ctgggaatc cttgttcga 
      gacaagaga aaaaggcca tatgttgat tcagcgtcc 
      agtgccccc agtgttaaa aaaacgaaa acagagatg 
      ccttctagg tcgtacaca ccgccacct gtcaaagat 
      tcccggctt aaattacca cttccacca ccgataggc 
      acggaccag gatggagat gagccgtac agtcctgag 
      gactcggac cccgacagc ataattccc gcaaccact 
      cgaagtgtt tccaacagt tcgacaatc tttctcgac 
      gctgacgcc gcggcaaca caagccttt caaccgttc 
      gttaacaag tttgaagag attccagga ctggataac 
      gcaactttg ccgtcaagt acgcaagaa atccaacgc 
      caaatggag aaactcaac aaagaaatc gaaatgcaa 
      aagtcggaa atcaacagt atatcgcaa aacatcgcc 
      actgcatcg aacgaaatc gggacttcg gctctggcg 
      aacatagcg ctcccgagc aatttgcag caaattctg 
      gacagcatt aaaacgatc ggttccact tcggcttta 
      gaatcgttg gagacgaag ccgcaagct gtggacgcg 
      ctgcaggaa agttccgac tttacgatt cctttgatg 
      ttgcctacg acgttttcg cgccattcc gccagttcc 
      gaaaatacc attcctttg aatttgcca agtaagaag 
      aaagtggca agcgacgct gagaaaacg agcgttttg 
      agttcgctg tctgaggag gatttgatt cgaaaggcg 
      gaagagatg cttgaagcg tccgaaaca acagacaag 
      ctagcaatt ccaccgagt gtttcaagt tcgtcacaa 
      atactgccc aaggttgag atgaaacaa ccgccaata 
      cctggactg gaggatgat gtctaa
    mRNA sequence
    • >TC014624 RA
      gttcttctt ccactcttg ttgttccat aaaatggcg 
      gtattgagc gagttgtgt tatttttag cgctttaaa 
      cacctcaaa ttcgagtaa ttggtacat gtgaacaac 
      ataatccac gattataaa tccgctgct ttgtatata 
      tcgtataaa tggagagtc cggtggact gttgaagct 
      tttccagca actacaaac aaaatatta aagtgagtt 
      cagtgccct gtgcaagaa cactaatgt ttggattgg 
      aaacttgag ccgtttgcg tgagatatg caccaagtg 
      ttaataatg tattaatgt gtgttttca tcgtcacac 
      gaacatcca aaggacagt gatttaaga cacttgctt 
      cgatgatga taaggttga agtcttgct gaaaacggc 
      tggtgtgac atcaagata caaaaggga tattgttgg 
      tcacgatgt caaacagcg tcgttaagg taacagatt 
      ccgattcaa caattgatg acaatttac ttcttctta 
      ttggtgaag atggaactg tcacaccga gtaaagacg 
      tcgtcaaag cttatttaa gtggcaaat cgggcgcca 
      ccaacctcc aagtaatgc atattaata aatcagata 
      atgagggca ttgatgttg cggtggatg acatgttat 
      atgtaccgg acgctgaag ctgacgacc ttccagata 
      ataaagaga cgtcttttg acgacatag cttacaatt 
      tatacaaca taaagcttg atcatgatt acacacctc 
      attgtgggt attctgagg atgatgagt tggcgcgaa 
      ctcttagta tattagacg gggacgaag acaatgact 
      ctccttccg tcacaaaac cactagaaa gtcgatccc 
      ttagaagca acgcacatg aaagcccca aacccaaaa 
      caaaggtag caacaccgc taaaaacaa tcaccaaag 
      atcaagcaa aaaagatta aaacaagga caaagagtg 
      agacatcga tcaaagacg gggaaagtc cgtcagatg 
      ctaaaaaag tcctcagac cggagcata aacccaagg 
      acaatgttg tcaagtctt tgaaaacaa taaagccag 
      tcgagcctg aatcaccta aagcggaaa agccgcaac 
      cagttgatc ccaagtcag ctgaaaaga aacagccca 
      agaaagatc gcaaagtgg cgaagccag tcccagacg 
      atttcgccc tgttttcaa cccctgata tcattagac 
      gagtgggag ggaaggaac caatttcgc ccgaactta 
      gcagtccgg atcaaaaca aaccggcga aaatcgaaa 
      ctcgatcga agtcagtct cagactccg ctcaagttt 
      cgcccaaac agaatcgct tgagtctcg acggcaaag 
      tagtcgata aatccccca gtaaaaaac cgactagcg 
      ataataaag cgaagaaaa tcatgtttg ataacacca 
      aaactgtgg cggaattgg aaccaaaac cgtgcgaaa 
      gtaacacga acgtggaac ctgcaatcc ccgagccaa 
      tgattgcga ataatgaag ataagtcgt ttccggctt 
      tacctctcg aaactcctg atttaagtg gaggtgaga 
      ataacatgg ctttggacg ggaatgggc tcgatcttg 
      acccaagct tgttggaaa acatcaata acgacctaa 
      tatctgaag atattctgt accaagttg cgcagtcgt 
      tggtgtcaa acacggagc tacagaacg cgatagata 
      agagcttga atgaggaga atttgggcc tcgattcga 
      gcgtccaag aggtgcagc aagacgacg tggttcaca 
      ataatcaaa cagagcagg taacgtcat ctgtgccac 
      aaaaaggaa ctcaaatcg tccgccctg acggtagga 
      tagtcgtaa tccctccaa tagaacgcc ccacaacca 
      ggagtcgaa acaagaaca aagatgccg ataaaccaa 
      ccccaaaat ttgtccaca aacccttgg atgaagagc 
      acgtctcag gcaacgaac tcgacagtt ctgaggaag 
      aagaggaag aatcggagg atgatccga acaaacttt 
      ggtgtattt gtaaccaac ctcacaaca accggttca 
      tgatctgtt gcgatacgt gcgaagaat ggtaccacg 
      gaaaatgcg tgaatatca caaaagcga tgggacagc 
      aaatggaag cggaaggaa gagaatgga tttgtctat 
      tttgtaaag atccacttt tgaagcgac cacaagccg 
      cggccagaa gaataagaa aagcttcgc ggaattcgc 
      gccagtcta cggacagct ctggcagta ccaaagtac 
      cagagacac ggccaagtt cagtcccgt gtgtcgttt 
      gccagaaac cgtcaagaa ctaattcga ttttctgca 
      gcgaaagct gcattttgg cgcacgcac aaggcgtcg 
      aacgagtgg tcgtgttcg agagatcaa ctggaaaca 
      tgttgaccg ggaacaagg cccccagtg ctgccaact 
      tggagcaat ggctcaaag aacacccag gttacgaag 
      tcgttcgtt ccgctggta aaatagtaa cgacaaagc 
      aaacgcaaa aaagtccgc catcgaaaa taaaaattt 
      taaaaggtt ccgaaacac ctgctatgc atttccaaa 
      atcgaatga aaaatttga ataaaaccg taccgaaaa 
      gtccacaag tggtcaaag tcggtaata catcaggtt 
      tgaaagttc ttaatcgac aaatttcga cggctcagc 
      tgagggaaa aggtcagta attcactta aagaggccg 
      catcaccta ccaagttta aaatcgtta caacttcga 
      ccaaatcga gtagttcgc cggcgaata gagaaaaac 
      ccacaacac ctaaagtac aaaaagtgc gaaatgcaa 
      gtacacctc aagccaaac aggagaata tcagagata 
      atgtcaaga aaacagttt acgagcagt tgaccaacc 
      gattaaaaa tggtggatg atttgaagt tgtctgagg 
      aggaattga aaaacattt cgctcgaaa ttgaatctc 
      aactgtaca aatgcttcg gcgatacgg ggcagaaat 
      acaggaaca aatacagaa gtttaattt ttaatatta 
      aagacataa agaatcaaa cactgtgga ggcggattt 
      gtgagaaga ccattaatc cgtaccaac tcgtcaggt 
      tatcgcccg acgacttag ccagccagg agttggccc 
      tttggcggg agcgcgaaa ccaaacacc agttagaca 
      tgattaaaa aatccgagc tcgaattac tccaatgca 
      acagacaat acgtgttga agacacaca aaggtgaac 
      aaattttgg aagacaatc gtccaacgg acaacctcg 
      acaacaccg aggtcataa caagtctga cagataacg 
      caacggggg agaaaaccg atcgtaaag acgacagaa 
      aaaaacaca gtcataggg ataaagagc ggaaatcga 
      cgaaagata agtccgaaa aaagtcatc acagtcaca 
      gatcgacga gtcgagatc acgatcgca gtcggaaac 
      gttcgcgca gcagggata ggagttcaa aacatgtgg 
      agaaacgga gatccagaa gcagggatc gacatagga 
      ggtcgtcac ataaaagtt caaggcata aaaaggagt 
      cgcatcgag ggtcgtcca ctgagaaac tcgataaaa 
      aatcgaagg agattttag aacagttgg ttgataata 
      aaattgtgc ctccgctgg aagacaggc tgtggaagc 
      atgtgccac aagaggacg ttttaccaa attcggttg 
      ctgaaagtg acagtgacc acgaaccga cttccactg 
      tcactattc caactccac cacgaaata acaacgatt 
      cggatgaat ttgcggctg gccacggtt tatcggaaa 
      ataaaaata atgataaat atcctgtaa aggagcaga 
      ctgaagtgg accactcga tgtcaccag tggttaagc 
      caaaagttg aggagattt ggcgtggta ccattaata 
      tggtagatg tggcacaaa tctcgatca cagctcacg 
      aagtgtcag gcgattgta gtggcttgg ccactgaac 
      tacccgcaa atttggaca ttgttggta gaatttccc 
      ccgataccg tgtgggact atatcggaa aaatgaaat 
      gttccaaca gtaaaatca tatcagtgg tgcgcttaa 
      acgcaatca atgtggaag aaaaaatgc cgtatttgg 
      ctctctaca gttacttat cgagcagga atcgactcg 
      gtgttgtta aaagtgtga acaaggccg tgaaagatt 
      tttacattt taccactgg cctctcaga aaccgatcc 
      cacaggctt tgttacctc tcaacggtc caggtttcg 
      aggaggcac ggcctgctt tgctcctgg gaatccttg 
      ttcgagaca agagaaaaa ggccatatg ttgattcag 
      cgtccagtg cccccagtg ttaaaaaaa cgaaaacag 
      agatgcctt ctaggtcgt acacaccgc cacctgtca 
      aagattccc ggcttaaat taccacttc caccaccga 
      taggcacgg accaggatg gagatgagc cgtacagtc 
      ctgaggact cggaccccg acagcataa ttcccgcaa 
      ccactcgaa gtgtttcca acagttcga caatctttc 
      tcgacgctg acgccgcgg caacacaag cctttcaac 
      cgttcgtta acaagtttg aagagattc caggactgg 
      ataacgcaa ctttgccgt caagtacgc aagaaatcc 
      aacgccaaa tggagaaac tcaacaaag aaatcgaaa 
      tgcaaaagt cggaaatca acagtatat cgcaaaaca 
      tcgccactg catcgaacg aaatcggga cttcggctc 
      tggcgaaca tagcgctcc cgagcaatt tgcagcaaa 
      ttctggaca gcattaaaa cgatcggtt ccacttcgg 
      ctttagaat cgttggaga cgaagccgc aagctgtgg 
      acgcgctgc aggaaagtt ccgacttta cgattcctt 
      tgatgttgc ctacgacgt tttcgcgcc attccgcca 
      gttccgaaa ataccattc ctttgaatt tgccaagta 
      agaagaaag tggcaagcg acgctgaga aaacgagcg 
      ttttgagtt cgctgtctg aggaggatt tgattcgaa 
      aggcggaag agatgcttg aagcgtccg aaacaacag 
      acaagctag caattccac cgagtgttt caagttcgt 
      cacaaatac tgcccaagg ttgagatga aacaaccgc 
      caatacctg gactggagg atgatgtct aattttggc 
      tggctgaag gaggtgttt cacaatgtt ttcgtcatt 
      tttataaga ataggactt tatttgagt aaaattgtt 
      ggaactttt atgtataga ctttagttg aaaactatg 
      gattcttat taattcact tacatgttt ttaatcagt 
      tttttatat tgtaaatat acaattgtt ttgtttcta 
      tgagacatg taaactagg aaaatacta cagataagt 
      gcaagcgat tattccatg taaattttg cgacctatg 
      ttaaattct gtatagtta cttttattt agaaaactt 
      gaaataaac catttcttt ttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY