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Gene information
General information
TC014442 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10467997..10472322
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014442
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07287

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014442 RA
      MSLGISSRL KHPKKHELM KSHSQAQIL ANKLRQDGV 
      LSTVENVLQ AVDQVAALL ASTAVDPNI SYKDSIINL 
      CQHLKVYGA YLEILYKEQ LDHAFKIFR DTAQDISRG 
      DILSRLHLL ELIELRAKG WKGTEVMDQ YYRRKANEY 
      VQIDMSDSA TSLNESMCS MTMSASPPL LGPGEVLKP 
      SGKFSKPTR IPGKKYCKD EVVIRNADS GKVNPGAKE 
      RLVQITGAN EESITHAKR LIEDTIRRN ASPVREHDM 
      AVGGPLTGS SSSINSSAS DDSALPSSA RASRALLHS 
      LSTNDANIG EYKYSVVTN GHTIKITGD NLDLVRTSK 
      LVLEEYFSG EPIHEQFYS FDSLPQPIV AGVEDGPLS 
      PPESIPSPG VITNGQSED SGKVVRKLR KPHTPIEEI 
      VKGKASPNE QLTKASLVY TIEFLAHLA MSPLCLTVP 
      NDWERISKE YPFLIRNNV ETFDAKQYL STRTGEPTC 
      VLSADEVDS SDV
    • >TC014442 RB
      MAVGGPLTG SSSSINSSA SDDSALPSS ARASRALLH 
      SLSTNDANI GEYKYSVVT NGHTIKITG DNLDLVRTS 
      KLVLEEYFS GEPIHEQFY SFDSLPQPI VAGVEDGPL 
      SPPESIPSP GVITNGQSE DSGKVVRKL RKPHTPIEE 
      IVKGKASPN EQLTKASLV YTIEFLAHL AMSPLCLTV 
      PNDWERISK EYPFLIRNN VETFDAKQY LSTRTGEPT 
      CVLSADEVD SSDV
    • >TC014442 RC
      MSLGISSRL KHPKKHELM KSHSQAQIL ANKLRQDGV 
      LSTVENVLQ AVDQVAALL ASTAVDPNI SYKDSIINL 
      CQHLKVYGA YLEILYKEQ LDHAFKIFR DTAQDISRG 
      DILSRLHLL ELIELRAKG WKGTEVMDQ YYRRKANEY 
      VQIDMSDSA TSLNESMCS MTMSASPPL LGPGEVLKP 
      SGKFSKPTR IPGKKYCKD EVVIRNADS GKGRCLHNR 
      HAFEARVIR DAMKNCVYL
    • >TC014442 RD
      MSLGISSRL KHPKKHELM KSHSQAQIL ANKLRQDGV 
      LSTVENVLQ AVDQVAALL ASTAVDPNI SYKDSIINL 
      CQHLKVYGA YLEILYKEQ LDHAFKIFR DTAQDISRG 
      DILSRLHLL ELIELRAKG WKGTEVMDQ YYRRKANEY 
      VQIDMSDSA TSLNESMCS MTMSASPPL LGPGEVLKP 
      SGKFSKPTR IPGKKYCKD EVVIRNADS GKVMGIKGR 
      RVHMIEELS ETIISFQRG KRLNTMLAF NPGAKERLV 
      QITGANEES ITHAKRLIE DTIRRNASP VREHDMAVG 
      GPLTGSSSS INSSASDDS ALPSSARAS RALLHSLST 
      NDANIGEYK YSVVTNGHT IKITGDNLD LVRTSKLVL 
      EEYFSGEPI HEQFYSFDS LPQPIVAGV EDGPLSPPE 
      SIPSPGVIT NGQSEDSGK VVRKLRKPH TPIEEIVKG 
      KASPNEQLT KASLVYTIE FLAHLAMSP LCLTVPNDW 
      ERISKEYPF LIRNNVETF DAKQYLSTR TGEPTCVLS 
      ADEVDSSDV
    CDS sequence
    • >TC014442 RA
      atgtctctg ggcatctcg tcccgactc aaacacccc 
      aagaagcac gaattgatg aaatcgcac agccaggcc 
      cagattctc gccaataag ttgcgacag gatggggtg 
      ctttcgaca gtggagaac gtgcttcaa gccgtcgat 
      caggtcgca gcgttgctc gcgtccacc gccgtggac 
      cccaacatt tcatacaag gatagcatt atcaatttg 
      tgccaacat ttaaaggtt tacggcgct tatttggaa 
      atactgtac aaggaacag ttggaccac gcatttaag 
      atttttcgg gacactgcc caggacatc agtcgaggc 
      gacatcctt tcgcggctg cacttgctc gaacttatt 
      gaactgagg gcgaaagga tggaagggc acagaagtg 
      atggaccag tattacagg cgaaaagcc aacgaatat 
      gtgcagatc gacatgtcc gactctgcg actagccta 
      aacgaatcg atgtgttct atgaccatg tcggcgtcg 
      ccgcccctt ttgggcccc ggtgaagtg ctgaagccc 
      tcggggaag ttcagcaag ccaactcga atcccgggt 
      aaaaagtat tgcaaggat gaggtggta atcaggaat 
      gcagattcg ggtaaagtc aatcccggt gccaaggag 
      agactggtt cagattacc ggagcgaac gaagaaagc 
      atcacccat gctaaacga ctaatcgaa gacacgatc 
      cgacggaac gcctcccct gttagagag cacgacatg 
      gccgtcggc ggccctcta acaggctcc agttctagt 
      atcaactct tctgcttct gatgatagt gctctgcca 
      tcgtcagcc agagcatca agagcttta ctgcacagt 
      ttgagcaca aacgacgcc aatatcggc gaatacaag 
      tacagcgtg gtcaccaat ggccacact atcaaaatc 
      accggtgat aacctcgat ttagttcga acgagcaaa 
      ttggttttg gaggagtac ttctcaggg gaaccaatc 
      cacgaacag ttttacagt ttcgatagt ctcccacaa 
      ccaatcgtc gcaggtgta gaggacggg ccgctctcc 
      ccacccgaa tccatcccc tctcccggg gtgatcaca 
      aacggacaa agcgaggac tcaggtaaa gttgtgagg 
      aaactgcgc aaaccccat acgcccatc gaagaaata 
      gtcaagggc aaggcgtcg cctaatgaa caattaact 
      aaggcgtcc ttggtgtac acgatcgaa tttttggcc 
      cacttggcc atgtcaccg ttgtgttta acggtaccg 
      aatgattgg gagcgaatc tcaaaggaa tacccgttt 
      ttaatccgc aataatgtg gagactttc gatgccaaa 
      cagtatcta tccacgcgt acaggcgaa cccacttgc 
      gtcctaagc gccgacgaa gtcgattcg tcagatgtt 
      tga
    • >TC014442 RB
      atggccgtc ggcggccct ctaacaggc tccagttct 
      agtatcaac tcttctgct tctgatgat agtgctctg 
      ccatcgtca gccagagca tcaagagct ttactgcac 
      agtttgagc acaaacgac gccaatatc ggcgaatac 
      aagtacagc gtggtcacc aatggccac actatcaaa 
      atcaccggt gataacctc gatttagtt cgaacgagc 
      aaattggtt ttggaggag tacttctca ggggaacca 
      atccacgaa cagttttac agtttcgat agtctccca 
      caaccaatc gtcgcaggt gtagaggac gggccgctc 
      tccccaccc gaatccatc ccctctccc ggggtgatc 
      acaaacgga caaagcgag gactcaggt aaagttgtg 
      aggaaactg cgcaaaccc catacgccc atcgaagaa 
      atagtcaag ggcaaggcg tcgcctaat gaacaatta 
      actaaggcg tccttggtg tacacgatc gaatttttg 
      gcccacttg gccatgtca ccgttgtgt ttaacggta 
      ccgaatgat tgggagcga atctcaaag gaatacccg 
      tttttaatc cgcaataat gtggagact ttcgatgcc 
      aaacagtat ctatccacg cgtacaggc gaacccact 
      tgcgtccta agcgccgac gaagtcgat tcgtcagat 
      gtttga
    • >TC014442 RC
      atgtctctg ggcatctcg tcccgactc aaacacccc 
      aagaagcac gaattgatg aaatcgcac agccaggcc 
      cagattctc gccaataag ttgcgacag gatggggtg 
      ctttcgaca gtggagaac gtgcttcaa gccgtcgat 
      caggtcgca gcgttgctc gcgtccacc gccgtggac 
      cccaacatt tcatacaag gatagcatt atcaatttg 
      tgccaacat ttaaaggtt tacggcgct tatttggaa 
      atactgtac aaggaacag ttggaccac gcatttaag 
      atttttcgg gacactgcc caggacatc agtcgaggc 
      gacatcctt tcgcggctg cacttgctc gaacttatt 
      gaactgagg gcgaaagga tggaagggc acagaagtg 
      atggaccag tattacagg cgaaaagcc aacgaatat 
      gtgcagatc gacatgtcc gactctgcg actagccta 
      aacgaatcg atgtgttct atgaccatg tcggcgtcg 
      ccgcccctt ttgggcccc ggtgaagtg ctgaagccc 
      tcggggaag ttcagcaag ccaactcga atcccgggt 
      aaaaagtat tgcaaggat gaggtggta atcaggaat 
      gcagattcg ggtaaaggt aggtgtctg cataaccgg 
      cacgcgttt gaggcgaga gtcattcga gatgcaatg 
      aaaaactgt gtttattta tag
    • >TC014442 RD
      atgtctctg ggcatctcg tcccgactc aaacacccc 
      aagaagcac gaattgatg aaatcgcac agccaggcc 
      cagattctc gccaataag ttgcgacag gatggggtg 
      ctttcgaca gtggagaac gtgcttcaa gccgtcgat 
      caggtcgca gcgttgctc gcgtccacc gccgtggac 
      cccaacatt tcatacaag gatagcatt atcaatttg 
      tgccaacat ttaaaggtt tacggcgct tatttggaa 
      atactgtac aaggaacag ttggaccac gcatttaag 
      atttttcgg gacactgcc caggacatc agtcgaggc 
      gacatcctt tcgcggctg cacttgctc gaacttatt 
      gaactgagg gcgaaagga tggaagggc acagaagtg 
      atggaccag tattacagg cgaaaagcc aacgaatat 
      gtgcagatc gacatgtcc gactctgcg actagccta 
      aacgaatcg atgtgttct atgaccatg tcggcgtcg 
      ccgcccctt ttgggcccc ggtgaagtg ctgaagccc 
      tcggggaag ttcagcaag ccaactcga atcccgggt 
      aaaaagtat tgcaaggat gaggtggta atcaggaat 
      gcagattcg ggtaaagta atgggtatc aaaggaagg 
      cgtgttcat atgattgaa gagcttagt gaaacgatc 
      atttcgttt cagcgaggt aaacgctta aacacaatg 
      ctcgctttc aatcccggt gccaaggag agactggtt 
      cagattacc ggagcgaac gaagaaagc atcacccat 
      gctaaacga ctaatcgaa gacacgatc cgacggaac 
      gcctcccct gttagagag cacgacatg gccgtcggc 
      ggccctcta acaggctcc agttctagt atcaactct 
      tctgcttct gatgatagt gctctgcca tcgtcagcc 
      agagcatca agagcttta ctgcacagt ttgagcaca 
      aacgacgcc aatatcggc gaatacaag tacagcgtg 
      gtcaccaat ggccacact atcaaaatc accggtgat 
      aacctcgat ttagttcga acgagcaaa ttggttttg 
      gaggagtac ttctcaggg gaaccaatc cacgaacag 
      ttttacagt ttcgatagt ctcccacaa ccaatcgtc 
      gcaggtgta gaggacggg ccgctctcc ccacccgaa 
      tccatcccc tctcccggg gtgatcaca aacggacaa 
      agcgaggac tcaggtaaa gttgtgagg aaactgcgc 
      aaaccccat acgcccatc gaagaaata gtcaagggc 
      aaggcgtcg cctaatgaa caattaact aaggcgtcc 
      ttggtgtac acgatcgaa tttttggcc cacttggcc 
      atgtcaccg ttgtgttta acggtaccg aatgattgg 
      gagcgaatc tcaaaggaa tacccgttt ttaatccgc 
      aataatgtg gagactttc gatgccaaa cagtatcta 
      tccacgcgt acaggcgaa cccacttgc gtcctaagc 
      gccgacgaa gtcgattcg tcagatgtt tga
    mRNA sequence
    • >TC014442 RA
      tcgatgctt gaacgatga atcgttcca tttatttat 
      aaaacgtcc gcaaggttc gaatttggc gccggcgag 
      ggcgcacgc gcgtgcaaa tttcgcatt cgcattgcg 
      tgcacggcc agctgtgcc agttgcgtc tgattttca 
      ataaataag taggaaaat aattacaag gtcaaaatt 
      tcccattca acgaaccga aaatgtctc tgggcatct 
      cgtcccgac tcaaacacc ccaagaagc acgaattga 
      tgaaatcgc acagccagg cccagattc tcgccaata 
      agttgcgac aggatgggg tgctttcga cagtggaga 
      acgtgcttc aagccgtcg atcaggtcg cagcgttgc 
      tcgcgtcca ccgccgtgg accccaaca tttcataca 
      aggatagca ttatcaatt tgtgccaac atttaaagg 
      tttacggcg cttatttgg aaatactgt acaaggaac 
      agttggacc acgcattta agatttttc gggacactg 
      cccaggaca tcagtcgag gcgacatcc tttcgcggc 
      tgcacttgc tcgaactta ttgaactga gggcgaaag 
      gatggaagg gcacagaag tgatggacc agtattaca 
      ggcgaaaag ccaacgaat atgtgcaga tcgacatgt 
      ccgactctg cgactagcc taaacgaat cgatgtgtt 
      ctatgacca tgtcggcgt cgccgcccc ttttgggcc 
      ccggtgaag tgctgaagc cctcgggga agttcagca 
      agccaactc gaatcccgg gtaaaaagt attgcaagg 
      atgaggtgg taatcagga atgcagatt cgggtaaag 
      tcaatcccg gtgccaagg agagactgg ttcagatta 
      ccggagcga acgaagaaa gcatcaccc atgctaaac 
      gactaatcg aagacacga tccgacgga acgcctccc 
      ctgttagag agcacgaca tggccgtcg gcggccctc 
      taacaggct ccagttcta gtatcaact cttctgctt 
      ctgatgata gtgctctgc catcgtcag ccagagcat 
      caagagctt tactgcaca gtttgagca caaacgacg 
      ccaatatcg gcgaataca agtacagcg tggtcacca 
      atggccaca ctatcaaaa tcaccggtg ataacctcg 
      atttagttc gaacgagca aattggttt tggaggagt 
      acttctcag gggaaccaa tccacgaac agttttaca 
      gtttcgata gtctcccac aaccaatcg tcgcaggtg 
      tagaggacg ggccgctct ccccacccg aatccatcc 
      cctctcccg gggtgatca caaacggac aaagcgagg 
      actcaggta aagttgtga ggaaactgc gcaaacccc 
      atacgccca tcgaagaaa tagtcaagg gcaaggcgt 
      cgcctaatg aacaattaa ctaaggcgt ccttggtgt 
      acacgatcg aatttttgg cccacttgg ccatgtcac 
      cgttgtgtt taacggtac cgaatgatt gggagcgaa 
      tctcaaagg aatacccgt ttttaatcc gcaataatg 
      tggagactt tcgatgcca aacagtatc tatccacgc 
      gtacaggcg aacccactt gcgtcctaa gcgccgacg 
      aagtcgatt cgtcagatg tttgacaca aatatcgaa 
      ctacctttt aagagaagc aattttttt ttcgtacga 
      aattttttc ttcgggctc aatatgttt attaacgtt 
      aggatagca aaaaatata aaaaaacgt tttatactc 
      tatggatag ttgcctatt tttcgtata cagtttgta 
      acattttta aaaaggttt gatgtgttg tgttttttg 
      acaagagtt taaaattgt tggtttagt aaaaaatct 
      attaactta agataaaaa caaaaaata cgagactac 
      ttattttta gttacttga ctgactgta ctaatctct 
      tagtggatc attcatttt ctcacttat tatttatat 
      gtgatcatt ataatcata taaggcagt ttctatgta 
      ttggattaa tatgtagtt ctggcatag gttaatgga 
      actagtgat atctgttga tggatcttc acggtattg 
      atattatta aacgaatga cgtgtacag agcacgact 
      tgcgatgta gtcttcttt tgttgttat tattaagca 
      tttgttttt cgtgattag tttttattt tttcgtaac 
      caatctggt catgtcgac tgttacctt aaccaaaag 
      tgttgtttt tcttttgta tacagttta tttattgcg 
      atcagatat ttaatgtct attatttcg cttcaagtt 
      taataaagt ttctgaatt aac
    • >TC014442 RB
      tgtgagtcg tttgtgttc gtttttcgg taaagtgat 
      tcgttttca gtcaatccc ggtgccaag gagagactg 
      gttcagatt accggagcg aacgaagaa agcatcacc 
      catgctaaa cgactaatc gaagacacg atccgacgg 
      aacgcctcc cctgttaga gagcacgac atggccgtc 
      ggcggccct ctaacaggc tccagttct agtatcaac 
      tcttctgct tctgatgat agtgctctg ccatcgtca 
      gccagagca tcaagagct ttactgcac agtttgagc 
      acaaacgac gccaatatc ggcgaatac aagtacagc 
      gtggtcacc aatggccac actatcaaa atcaccggt 
      gataacctc gatttagtt cgaacgagc aaattggtt 
      ttggaggag tacttctca ggggaacca atccacgaa 
      cagttttac agtttcgat agtctccca caaccaatc 
      gtcgcaggt gtagaggac gggccgctc tccccaccc 
      gaatccatc ccctctccc ggggtgatc acaaacgga 
      caaagcgag gactcaggt aaagttgtg aggaaactg 
      cgcaaaccc catacgccc atcgaagaa atagtcaag 
      ggcaaggcg tcgcctaat gaacaatta actaaggcg 
      tccttggtg tacacgatc gaatttttg gcccacttg 
      gccatgtca ccgttgtgt ttaacggta ccgaatgat 
      tgggagcga atctcaaag gaatacccg tttttaatc 
      cgcaataat gtggagact ttcgatgcc aaacagtat 
      ctatccacg cgtacaggc gaacccact tgcgtccta 
      agcgccgac gaagtcgat tcgtcagat gtttgacac 
      aaatatcga actaccttt taagagaag caatttttt 
      tttcgtacg aaatttttt cttcgggct caatatgtt 
      tattaacgt taggatagc aaaaaatat aaaaaaacg 
      ttttatact ctatggata gttgcctat ttttcgtat 
      acagtttgt aacattttt aaaaaggtt tgatgtgtt 
      gtgtttttt gacaagagt ttaaaattg ttggtttag 
      taaaaaatc tattaactt aagataaaa acaaaaaat 
      acgagacta cttattttt agttacttg actgactgt 
      actaatctc ttagtggat cattcattt tctcactta 
      ttatttata tgtgatcat tataatcat ataaggcag 
      tttctatgt attggatta atatgtagt tctggcata 
      ggttaatgg aactagtga tatctgttg atggatctt 
      cacggtatt gatattatt aaacgaatg acgtgtaca 
      gagcacgac ttgcgatgt agtcttctt ttgttgtta 
      ttattaagc atttgtttt tcgtgatta gtttttatt 
      ttttcgtaa ccaatctgg tcatgtcga ctgttacct 
      taaccaaaa gtgttgttt ttcttttgt atacagttt 
      atttattgc gatcagata tttaatgtc tattatttc 
      gcttcaagt ttaataaag tttctgaat taac
    • >TC014442 RC
      tcgatgctt gaacgatga atcgttcca tttatttat 
      aaaacgtcc gcaaggttc gaatttggc gccggcgag 
      ggcgcacgc gcgtgcaaa tttcgcatt cgcattgcg 
      tgcacggcc agctgtgcc agttgcgtc tgattttca 
      ataaataag taggaaaat aattacaag gtcaaaatt 
      tcccattca acgaaccga aaatgtctc tgggcatct 
      cgtcccgac tcaaacacc ccaagaagc acgaattga 
      tgaaatcgc acagccagg cccagattc tcgccaata 
      agttgcgac aggatgggg tgctttcga cagtggaga 
      acgtgcttc aagccgtcg atcaggtcg cagcgttgc 
      tcgcgtcca ccgccgtgg accccaaca tttcataca 
      aggatagca ttatcaatt tgtgccaac atttaaagg 
      tttacggcg cttatttgg aaatactgt acaaggaac 
      agttggacc acgcattta agatttttc gggacactg 
      cccaggaca tcagtcgag gcgacatcc tttcgcggc 
      tgcacttgc tcgaactta ttgaactga gggcgaaag 
      gatggaagg gcacagaag tgatggacc agtattaca 
      ggcgaaaag ccaacgaat atgtgcaga tcgacatgt 
      ccgactctg cgactagcc taaacgaat cgatgtgtt 
      ctatgacca tgtcggcgt cgccgcccc ttttgggcc 
      ccggtgaag tgctgaagc cctcgggga agttcagca 
      agccaactc gaatcccgg gtaaaaagt attgcaagg 
      atgaggtgg taatcagga atgcagatt cgggtaaag 
      gtaggtgtc tgcataacc ggcacgcgt ttgaggcga 
      gagtcattc gagatgcaa tgaaaaact gtgtttatt 
      tatagtaat gggtatcaa aggaaggcg tgttcatat 
      gattgaaga gcttagtga aacgatcat ttcgtttca 
      gcgaggtaa acgcttaaa cacaatgct cgcttgtct 
      ttcttaacg agtacttgg cactaaatc tgtgttttt 
      gttttaatt atgattttt aacgactaa ttatcacct 
      agtcgtgca ggtcgatag cgcttcacc attcatttt 
      ttattagcc gtaatattc taaacaagg gcatgatcg 
      caaataata ccatcattg tgtg
    • >TC014442 RD
      tcgatgctt gaacgatga atcgttcca tttatttat 
      aaaacgtcc gcaaggttc gaatttggc gccggcgag 
      ggcgcacgc gcgtgcaaa tttcgcatt cgcattgcg 
      tgcacggcc agctgtgcc agttgcgtc tgattttca 
      ataaataag taggaaaat aattacaag gtcaaaatt 
      tcccattca acgaaccga aaatgtctc tgggcatct 
      cgtcccgac tcaaacacc ccaagaagc acgaattga 
      tgaaatcgc acagccagg cccagattc tcgccaata 
      agttgcgac aggatgggg tgctttcga cagtggaga 
      acgtgcttc aagccgtcg atcaggtcg cagcgttgc 
      tcgcgtcca ccgccgtgg accccaaca tttcataca 
      aggatagca ttatcaatt tgtgccaac atttaaagg 
      tttacggcg cttatttgg aaatactgt acaaggaac 
      agttggacc acgcattta agatttttc gggacactg 
      cccaggaca tcagtcgag gcgacatcc tttcgcggc 
      tgcacttgc tcgaactta ttgaactga gggcgaaag 
      gatggaagg gcacagaag tgatggacc agtattaca 
      ggcgaaaag ccaacgaat atgtgcaga tcgacatgt 
      ccgactctg cgactagcc taaacgaat cgatgtgtt 
      ctatgacca tgtcggcgt cgccgcccc ttttgggcc 
      ccggtgaag tgctgaagc cctcgggga agttcagca 
      agccaactc gaatcccgg gtaaaaagt attgcaagg 
      atgaggtgg taatcagga atgcagatt cgggtaaag 
      taatgggta tcaaaggaa ggcgtgttc atatgattg 
      aagagctta gtgaaacga tcatttcgt ttcagcgag 
      gtaaacgct taaacacaa tgctcgctt tcaatcccg 
      gtgccaagg agagactgg ttcagatta ccggagcga 
      acgaagaaa gcatcaccc atgctaaac gactaatcg 
      aagacacga tccgacgga acgcctccc ctgttagag 
      agcacgaca tggccgtcg gcggccctc taacaggct 
      ccagttcta gtatcaact cttctgctt ctgatgata 
      gtgctctgc catcgtcag ccagagcat caagagctt 
      tactgcaca gtttgagca caaacgacg ccaatatcg 
      gcgaataca agtacagcg tggtcacca atggccaca 
      ctatcaaaa tcaccggtg ataacctcg atttagttc 
      gaacgagca aattggttt tggaggagt acttctcag 
      gggaaccaa tccacgaac agttttaca gtttcgata 
      gtctcccac aaccaatcg tcgcaggtg tagaggacg 
      ggccgctct ccccacccg aatccatcc cctctcccg 
      gggtgatca caaacggac aaagcgagg actcaggta 
      aagttgtga ggaaactgc gcaaacccc atacgccca 
      tcgaagaaa tagtcaagg gcaaggcgt cgcctaatg 
      aacaattaa ctaaggcgt ccttggtgt acacgatcg 
      aatttttgg cccacttgg ccatgtcac cgttgtgtt 
      taacggtac cgaatgatt gggagcgaa tctcaaagg 
      aatacccgt ttttaatcc gcaataatg tggagactt 
      tcgatgcca aacagtatc tatccacgc gtacaggcg 
      aacccactt gcgtcctaa gcgccgacg aagtcgatt 
      cgtcagatg tttgacaca aatatcgaa ctacctttt 
      aagagaagc aattttttt ttcgtacga aattttttc 
      ttcgggctc aatatgttt attaacgtt aggatagca 
      aaaaatata aaaaaacgt tttatactc tatggatag 
      ttgcctatt tttcgtata cagtttgta acattttta 
      aaaaggttt gatgtgttg tgttttttg acaagagtt 
      taaaattgt tggtttagt aaaaaatct attaactta 
      agataaaaa caaaaaata cgagactac ttattttta 
      gttacttga ctgactgta ctaatctct tagtggatc 
      attcatttt ctcacttat tatttatat gtgatcatt 
      ataatcata taaggcagt ttctatgta ttggattaa 
      tatgtagtt ctggcatag gttaatgga actagtgat 
      atctgttga tggatcttc acggtattg atattatta 
      aacgaatga cgtgtacag agcacgact tgcgatgta 
      gtcttcttt tgttgttat tattaagca tttgttttt 
      cgtgattag tttttattt tttcgtaac caatctggt 
      catgtcgac tgttacctt aaccaaaag tgttgtttt 
      tcttttgta tacagttta tttattgcg atcagatat 
      ttaatgtct attatttcg cttcaagtt taataaagt 
      ttctgaatt aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY